DK3783102T5 - Amylasemutant, der har høj specifik aktivitet og termisk stabilitet, gen af mutanten og anvendelser deraf - Google Patents
Amylasemutant, der har høj specifik aktivitet og termisk stabilitet, gen af mutanten og anvendelser deraf Download PDFInfo
- Publication number
- DK3783102T5 DK3783102T5 DK19770537.9T DK19770537T DK3783102T5 DK 3783102 T5 DK3783102 T5 DK 3783102T5 DK 19770537 T DK19770537 T DK 19770537T DK 3783102 T5 DK3783102 T5 DK 3783102T5
- Authority
- DK
- Denmark
- Prior art keywords
- mutant
- gene
- thermal stability
- specific activity
- high specific
- Prior art date
Links
- 239000004382 Amylase Substances 0.000 title 1
- 102000013142 Amylases Human genes 0.000 title 1
- 108010065511 Amylases Proteins 0.000 title 1
- 235000019418 amylase Nutrition 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01001—Alpha-amylase (3.2.1.1)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810235559 | 2018-03-21 | ||
| PCT/CN2019/079000 WO2019179485A1 (zh) | 2018-03-21 | 2019-03-21 | 具有高比活及热稳定性的淀粉酶突变体及其基因和应用 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DK3783102T3 DK3783102T3 (da) | 2023-12-18 |
| DK3783102T5 true DK3783102T5 (da) | 2024-08-26 |
Family
ID=64196899
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK19770537.9T DK3783102T5 (da) | 2018-03-21 | 2019-03-21 | Amylasemutant, der har høj specifik aktivitet og termisk stabilitet, gen af mutanten og anvendelser deraf |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US11377647B2 (da) |
| EP (1) | EP3783102B1 (da) |
| CN (1) | CN108841809A (da) |
| DK (1) | DK3783102T5 (da) |
| WO (1) | WO2019179485A1 (da) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108841809A (zh) * | 2018-03-21 | 2018-11-20 | 中国农业科学院饲料研究所 | 具有高比活及热稳定性的淀粉酶突变体及其基因和应用 |
| CN109810961B (zh) * | 2019-03-20 | 2019-10-18 | 中粮集团有限公司 | 用于高浓度淀粉液化的a-淀粉酶突变体及其编码基因和它们的应用 |
| CN109897842B (zh) * | 2019-03-25 | 2020-12-11 | 中国农业科学院北京畜牧兽医研究所 | 淀粉酶突变体zdamya及其编码基因和应用 |
| PE20251535A1 (es) | 2022-05-14 | 2025-06-05 | Novozymes As | Composiciones y metodos para prevenir, tratar, suprimir y/o eliminar infecciones e infestaciones fitopatogenas |
| CN118207187B (zh) * | 2024-05-22 | 2024-08-30 | 潍坊康地恩生物科技有限公司 | 高比活淀粉酶突变体 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6887986B1 (en) * | 1998-11-16 | 2005-05-03 | Novozymes A/S | α-amylase variants |
| WO2014007921A1 (en) * | 2012-06-08 | 2014-01-09 | Danisco Us Inc. | Variant alpha amylases with enhanced activity on starch polymers |
| US20160017305A1 (en) * | 2013-03-11 | 2016-01-21 | Danisco Us Inc. | Alpha-amylase combinatorial variants |
| WO2014183920A1 (en) * | 2013-05-17 | 2014-11-20 | Novozymes A/S | Polypeptides having alpha amylase activity |
| CN104263708B (zh) * | 2013-09-17 | 2017-02-15 | 江南大学 | 一种热稳定性提高的淀粉酶突变体及其制备方法和应用 |
| CN108841809A (zh) * | 2018-03-21 | 2018-11-20 | 中国农业科学院饲料研究所 | 具有高比活及热稳定性的淀粉酶突变体及其基因和应用 |
-
2018
- 2018-07-23 CN CN201810812153.4A patent/CN108841809A/zh active Pending
-
2019
- 2019-03-21 DK DK19770537.9T patent/DK3783102T5/da active
- 2019-03-21 US US16/982,628 patent/US11377647B2/en active Active
- 2019-03-21 WO PCT/CN2019/079000 patent/WO2019179485A1/zh not_active Ceased
- 2019-03-21 EP EP19770537.9A patent/EP3783102B1/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20210024908A1 (en) | 2021-01-28 |
| EP3783102B1 (en) | 2023-09-13 |
| CN108841809A (zh) | 2018-11-20 |
| EP3783102A1 (en) | 2021-02-24 |
| DK3783102T3 (da) | 2023-12-18 |
| WO2019179485A1 (zh) | 2019-09-26 |
| US11377647B2 (en) | 2022-07-05 |
| EP3783102A4 (en) | 2022-02-23 |
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