DK3224356T3 - Metoder til rekombinant udtrykkelse af beta-glucosidase-gen - Google Patents
Metoder til rekombinant udtrykkelse af beta-glucosidase-gen Download PDFInfo
- Publication number
- DK3224356T3 DK3224356T3 DK15862373.6T DK15862373T DK3224356T3 DK 3224356 T3 DK3224356 T3 DK 3224356T3 DK 15862373 T DK15862373 T DK 15862373T DK 3224356 T3 DK3224356 T3 DK 3224356T3
- Authority
- DK
- Denmark
- Prior art keywords
- beta
- methods
- recombinant expression
- glucosidase gene
- glucosidase
- Prior art date
Links
- 108010047754 beta-Glucosidase Proteins 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 238000003259 recombinant expression Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2445—Beta-glucosidase (3.2.1.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01021—Beta-glucosidase (3.2.1.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/23—Aspartic endopeptidases (3.4.23)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/23—Aspartic endopeptidases (3.4.23)
- C12Y304/23023—Mucorpepsin (3.4.23.23)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410699758.9A CN105695439B (zh) | 2014-11-27 | 2014-11-27 | 一种β-葡萄糖苷酶基因的重组表达方法 |
| PCT/CN2015/000826 WO2016082305A1 (en) | 2014-11-27 | 2015-11-27 | Methods for recombinant expression of beta-glucosidase gene |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3224356T3 true DK3224356T3 (da) | 2020-07-13 |
Family
ID=56073431
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK15862373.6T DK3224356T3 (da) | 2014-11-27 | 2015-11-27 | Metoder til rekombinant udtrykkelse af beta-glucosidase-gen |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US9969995B2 (da) |
| EP (1) | EP3224356B9 (da) |
| CN (1) | CN105695439B (da) |
| DK (1) | DK3224356T3 (da) |
| ES (1) | ES2805092T3 (da) |
| WO (1) | WO2016082305A1 (da) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106818885B (zh) * | 2017-02-20 | 2021-05-14 | 华南理工大学 | 一种酸性蛋白酶在制备抑制病原菌制剂中的应用 |
| CN106834336B (zh) * | 2017-02-20 | 2020-12-22 | 华南理工大学 | 一种哈茨木霉酸性蛋白酶p6281的异源表达及纯化方法 |
| CN106978409B (zh) * | 2017-04-26 | 2020-09-11 | 福建福大百特生物科技有限公司 | 一种α-葡萄糖苷酶的高效制备方法 |
| CN107365780A (zh) * | 2017-08-10 | 2017-11-21 | 广东省生物资源应用研究所 | 一种黄胸散白蚁唾液腺β‑糖苷酶基因的克隆及原核表达方法 |
| CN111876434B (zh) * | 2020-07-23 | 2023-05-16 | 江西省科学院微生物研究所 | 密码子优化的β-葡萄糖苷酶基因及其表达载体 |
| CN113403209B (zh) * | 2021-07-30 | 2022-08-26 | 西南大学 | 天冬氨酸蛋白酶基因在改良球孢白僵菌菌株中的应用 |
| CN114276954B (zh) * | 2021-12-07 | 2023-09-26 | 广西大学 | 一株发根农杆菌菌株at13及其应用 |
| CN118006573A (zh) * | 2024-01-18 | 2024-05-10 | 华东理工大学 | 一种还原糖氧化酶及其分泌表达菌株的构建和应用 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6268196B1 (en) * | 1993-12-17 | 2001-07-31 | Genencor International, Inc. | Method and compositions for treating cellulose containing fabrics using truncated cellulase enzyme compositions |
| CA2838451C (en) * | 2004-03-25 | 2017-10-24 | Novozymes, Inc. | Methods for degrading or converting plant cell wall polysaccharides |
| BRPI0714870A2 (pt) | 2006-07-21 | 2013-05-28 | Novozymes Inc | mÉtodo para produzir um polipeptÍdeo secretado tendo atividade biolàgica, proteÍna de fusço isolada, polinucleotÍdeo isolado, construÇço de proteÍna de fusço, vetor de expressço, cÉlula hospedeira féngica, mÉtodos para degradar ou conventer um material celulàsico e para produzir uma substÂncia |
| EP2250257A1 (en) | 2008-03-07 | 2010-11-17 | Danisco US Inc. | Expression of catalase in trichoderma |
| US8900847B2 (en) * | 2009-09-30 | 2014-12-02 | Codexis, Inc. | Polynucleotides encoding recombinant C1 beta-glucosidase for production of sugars from cellulosic biomass |
| JP2011205992A (ja) * | 2010-03-30 | 2011-10-20 | National Institute Of Advanced Industrial Science & Technology | グルコース存在下で活性を増加するβ−グルコシダーゼ |
| CN103339252A (zh) * | 2010-11-18 | 2013-10-02 | 诺维信股份有限公司 | 具有纤维素分解增强活性的嵌合多肽及其编码多核苷酸 |
| SG190321A1 (en) * | 2010-11-24 | 2013-06-28 | Novartis Int Pharm Ltd | Fusion enzymes having n-acetylglucosaminyltransferase activity |
| CN102220369B (zh) | 2011-05-11 | 2012-10-10 | 天津大学 | 里氏木霉β-葡萄糖苷酶基因BGL1的重组载体、重组菌及在重组菌中的表达 |
| DK2791350T3 (da) * | 2011-12-13 | 2019-05-06 | Danisco Us Inc | Enzymcocktails fremstillet af blandede kulturer |
| WO2014145768A2 (en) * | 2013-03-15 | 2014-09-18 | Bp Corporation North America Inc. | Use of non-fungal 5' utrs in filamentous fungi |
-
2014
- 2014-11-27 CN CN201410699758.9A patent/CN105695439B/zh active Active
-
2015
- 2015-11-27 WO PCT/CN2015/000826 patent/WO2016082305A1/en not_active Ceased
- 2015-11-27 EP EP15862373.6A patent/EP3224356B9/en active Active
- 2015-11-27 US US15/529,657 patent/US9969995B2/en active Active
- 2015-11-27 DK DK15862373.6T patent/DK3224356T3/da active
- 2015-11-27 ES ES15862373T patent/ES2805092T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP3224356B1 (en) | 2020-04-29 |
| EP3224356A4 (en) | 2018-05-09 |
| CN105695439B (zh) | 2023-08-01 |
| WO2016082305A1 (en) | 2016-06-02 |
| CN105695439A (zh) | 2016-06-22 |
| EP3224356A1 (en) | 2017-10-04 |
| US20170327807A1 (en) | 2017-11-16 |
| US9969995B2 (en) | 2018-05-15 |
| EP3224356B9 (en) | 2020-11-04 |
| ES2805092T3 (es) | 2021-02-10 |
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