DK3274093T3 - Mikrofluidisk sorteringsindretning - Google Patents
Mikrofluidisk sorteringsindretning Download PDFInfo
- Publication number
- DK3274093T3 DK3274093T3 DK16713384.2T DK16713384T DK3274093T3 DK 3274093 T3 DK3274093 T3 DK 3274093T3 DK 16713384 T DK16713384 T DK 16713384T DK 3274093 T3 DK3274093 T3 DK 3274093T3
- Authority
- DK
- Denmark
- Prior art keywords
- sorting
- samples
- braille
- sorting device
- valves
- Prior art date
Links
- 238000000034 method Methods 0.000 claims description 17
- 239000012530 fluid Substances 0.000 claims description 16
- 238000004458 analytical method Methods 0.000 claims description 13
- 238000003384 imaging method Methods 0.000 claims description 7
- 230000001419 dependent effect Effects 0.000 claims description 3
- 229920005570 flexible polymer Polymers 0.000 claims description 3
- 229920000642 polymer Polymers 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims 6
- 210000004027 cell Anatomy 0.000 description 43
- 210000002257 embryonic structure Anatomy 0.000 description 7
- 238000000926 separation method Methods 0.000 description 6
- 238000004163 cytometry Methods 0.000 description 5
- 238000012216 screening Methods 0.000 description 5
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 4
- 238000004113 cell culture Methods 0.000 description 4
- 239000004205 dimethyl polysiloxane Substances 0.000 description 4
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 4
- 210000004408 hybridoma Anatomy 0.000 description 4
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 4
- 239000002356 single layer Substances 0.000 description 4
- 239000003086 colorant Substances 0.000 description 3
- 238000002032 lab-on-a-chip Methods 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 3
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 101100180402 Caenorhabditis elegans jun-1 gene Proteins 0.000 description 2
- 240000002834 Paulownia tomentosa Species 0.000 description 2
- 235000010678 Paulownia tomentosa Nutrition 0.000 description 2
- 238000001574 biopsy Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000010226 confocal imaging Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 238000001506 fluorescence spectroscopy Methods 0.000 description 1
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000003760 hair shine Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- -1 polydimethylsiloxane Polymers 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1425—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0652—Sorting or classification of particles or molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0633—Valves, specific forms thereof with moving parts
- B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Clinical Laboratory Science (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Fluid Mechanics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Claims (14)
1. Sorteringsindretning (10) omfattende: en kanal (40), som er indrettet til at tillade passage af en flerhed af prøver i en væske til en første side af et sorteringssted (50); en flerhed af Braille-ventiler (95), som er forbundet med en flerhed af forbindelsesdele til sorteringsstedet (50) og anbragt nedstrøms i en afstand til sorteringsstedet (50); en analyseindretning (80) til analyse af nogle af flerheden af prøverne og indrettet til at styre nogle af flerheden af Braille-ventiler (95) afhængigt af egenskaberne af de analyserede af flerheden af prøverne; kendetegnet ved, at analyseindretningen (80) er monteret ved sorteringsstedet (50).
2. Sorteringsindretning ifølge krav 1, hvor Braille-ventilerne (95) er anbragt nedstrøms for sorteringsstedet (50) i en afstand på mindst 3000 pm.
3. Sorteringsindretning (10) ifølge krav 1 eller 2, hvor sorteringsstedet (50) og flerheden af Braille-ventiler (95) er anbragt på to forskellige mikrofluidiske chips, som er forbundet ved hjælp af rør (96).
4. Sorteringsindretning (10) ifølge et hvilket som helst af de foregående krav, hvor væsken injiceres af eksterne pumper (35).
5. Sorteringsindretning (10) ifølge et hvilket som helst af de foregående krav, der yderligere omfatter en mikrotiterplade (300) med brønde (310), som er væskeforbundet til forbindelser, der kan styres af mindst én af Braille-ventilerne (95).
6. Sorteringsindretning (10) ifølge et hvilket som helst af de foregående krav, hvor i det mindste dele af sorteringsindretningen (10) er fremstillet af en komprimerbar eller ekspanderbar polymer.
7. Sorteringsindretning (10) ifølge et hvilket som helst af de foregående krav, hvor analyseindretningen (80) er ét af et kamera, et mikroskop eller et fotomultiplikatorrør.
8. Sorteringsindretning (10) ifølge et hvilket som helst af de foregående krav, hvor kanalen er fremstillet afen fleksibel polymer.
9. Fremgangsmåde til sortering af en flerhed af prøver i en væske i en kanal (40), hvilken fremgangsmåde omfatter: passage (420) af væsken med flerheden af prøver langs med kanalen (40); analyse (450) ved et sorteringssted (50) af nogle af flerheden af prøver for at bestemme mindst én egenskab af nogle af prøverne; åbning af én af en flerhed af Braille-ventiler (95), der er anbragt nedstrøms i en afstand til sorteringsstedet (50), idet den åbne af flerheden af Braille-ventiler (95) er afhængig af den mindst ene egenskab; og at tillade (460) den analyserede prøve at komme ind i én af en flerhed af opsamlingskanaler (110), der er forbundet med den åbne Braille-ventil (95).
10. Fremgangsmåde ifølge krav 9, der yderligere omfatter afbrydelse (440) af væskestrømmen under analysen af prøven.
11. Fremgangsmåde ifølge krav 9 eller 10, der yderligere omfatter injektion af væsken ind i kanalen (40).
12. Fremgangsmåde ifølge krav 11, der yderligere omfatter lukning af hele flerheden af Braille-ventiler (95), mens der stadig injiceres væske ind i kanalen.
13. Fremgangsmåde ifølge et hvilket som helst af kravene 9 til 12, der yderligere omfatter passage af en flerhed af de analyserede prøver med lignende egenskaber langs med den samme af flerheden af opsamlingskanaler (110).
14. Fremgangsmåde ifølge et af kravene 9 til 13, hvor analysen (450) af nogle af prøverne omfatter billeddannelse (430) af prøven og analyse (450) af billederne af prøven.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP15160580.5A EP3072595A1 (en) | 2015-03-24 | 2015-03-24 | Microfluidic sorting device |
| PCT/EP2016/056637 WO2016151107A1 (en) | 2015-03-24 | 2016-03-24 | Microfluidic sorting device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3274093T3 true DK3274093T3 (da) | 2019-04-23 |
Family
ID=52780858
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK16713384.2T DK3274093T3 (da) | 2015-03-24 | 2016-03-24 | Mikrofluidisk sorteringsindretning |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US10569272B2 (da) |
| EP (2) | EP3072595A1 (da) |
| DK (1) | DK3274093T3 (da) |
| ES (1) | ES2718751T3 (da) |
| PT (1) | PT3274093T (da) |
| WO (1) | WO2016151107A1 (da) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CN117143960A (zh) | 2017-05-18 | 2023-12-01 | 10X基因组学有限公司 | 用于分选液滴和珠的方法和系统 |
| US10544413B2 (en) | 2017-05-18 | 2020-01-28 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| US20190064173A1 (en) | 2017-08-22 | 2019-02-28 | 10X Genomics, Inc. | Methods of producing droplets including a particle and an analyte |
| WO2019083852A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING |
| US12180440B2 (en) | 2018-06-01 | 2024-12-31 | Sony Corporation | Microchip and sample sorting kit |
| WO2020176882A1 (en) | 2019-02-28 | 2020-09-03 | 10X Genomics, Inc. | Devices, systems, and methods for increasing droplet formation efficiency |
| AU2020237460A1 (en) * | 2019-03-11 | 2021-10-07 | Isolation Bio Inc. | Method of selecting microorganism isolates on a high-density growth platform |
| CN109837204B (zh) * | 2019-04-12 | 2023-06-16 | 南京林业大学 | 一种集成细胞分选聚焦的微流控芯片检测系统及方法 |
| US12186751B2 (en) | 2019-06-28 | 2025-01-07 | 10X Genomics, Inc. | Devices and systems incorporating acoustic ordering and methods of use thereof |
| US12059679B2 (en) | 2019-11-19 | 2024-08-13 | 10X Genomics, Inc. | Methods and devices for sorting droplets and particles |
| US20210237064A1 (en) * | 2020-01-31 | 2021-08-05 | Astrin Biosciences, Inc. | Biological Fluid Filtration System |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030175980A1 (en) * | 2002-03-14 | 2003-09-18 | Hayenga Jon W. | Ribbon flow cytometry and cell sorting |
| EP2261650A3 (en) * | 2004-09-15 | 2011-07-06 | IntegenX Inc. | Microfluidic devices |
| US20070243627A1 (en) * | 2004-09-30 | 2007-10-18 | Shuichi Takayama | Computerized control method and system for microfluidics and computer program product for use therein |
| EP2029781A4 (en) * | 2006-05-26 | 2010-06-30 | Althea Technologies Inc | BIOCHEMICAL ANALYSIS OF PARTITIONED CELLS |
| CA2659046A1 (en) * | 2006-08-02 | 2008-02-07 | The Regents Of The University Of Michigan | Multiplexed microfluidic valve activator |
| US7807454B2 (en) * | 2006-10-18 | 2010-10-05 | The Regents Of The University Of California | Microfluidic magnetophoretic device and methods for using the same |
| WO2014138203A2 (en) * | 2013-03-05 | 2014-09-12 | Board Of Regents, The University Of Texas System | Microfluidic devices for the rapid and automated processing of sample populations |
| KR102449743B1 (ko) * | 2014-08-14 | 2022-09-29 | 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 | 마이크로유체 디바이스들의 출력을 분석하기 위한 장치 및 방법 |
-
2015
- 2015-03-24 EP EP15160580.5A patent/EP3072595A1/en not_active Withdrawn
-
2016
- 2016-03-24 WO PCT/EP2016/056637 patent/WO2016151107A1/en not_active Ceased
- 2016-03-24 DK DK16713384.2T patent/DK3274093T3/da active
- 2016-03-24 US US15/561,404 patent/US10569272B2/en active Active
- 2016-03-24 PT PT16713384T patent/PT3274093T/pt unknown
- 2016-03-24 ES ES16713384T patent/ES2718751T3/es active Active
- 2016-03-24 EP EP16713384.2A patent/EP3274093B1/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| ES2718751T3 (es) | 2019-07-04 |
| PT3274093T (pt) | 2019-04-08 |
| US20180104691A1 (en) | 2018-04-19 |
| EP3072595A1 (en) | 2016-09-28 |
| WO2016151107A1 (en) | 2016-09-29 |
| US10569272B2 (en) | 2020-02-25 |
| EP3274093B1 (en) | 2019-01-02 |
| EP3274093A1 (en) | 2018-01-31 |
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