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DK2992114T3 - Berigelse af DNA-sekvensering biblioteker fra prøver indeholdende små mængder af mål-DNA - Google Patents

Berigelse af DNA-sekvensering biblioteker fra prøver indeholdende små mængder af mål-DNA Download PDF

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Publication number
DK2992114T3
DK2992114T3 DK14794007.6T DK14794007T DK2992114T3 DK 2992114 T3 DK2992114 T3 DK 2992114T3 DK 14794007 T DK14794007 T DK 14794007T DK 2992114 T3 DK2992114 T3 DK 2992114T3
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DK
Denmark
Prior art keywords
enrichment
small amounts
samples containing
dna
containing small
Prior art date
Application number
DK14794007.6T
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English (en)
Inventor
Meredith L Carpenter
Jason D Buenrostro
William J Greenleaf
Carlos D Bustamante
Original Assignee
Univ Leland Stanford Junior
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Publication of DK2992114T3 publication Critical patent/DK2992114T3/da

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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583Features relative to the processes being carried out
    • B01J2219/00585Parallel processes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583Features relative to the processes being carried out
    • B01J2219/00596Solid-phase processes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718Type of compounds synthesised
    • B01J2219/0072Organic compounds
    • B01J2219/00722Nucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK14794007.6T 2013-05-04 2014-05-02 Berigelse af DNA-sekvensering biblioteker fra prøver indeholdende små mængder af mål-DNA DK2992114T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361819564P 2013-05-04 2013-05-04
PCT/US2014/036670 WO2014182574A1 (en) 2013-05-04 2014-05-02 Enrichment of dna sequencing libraries from samples containing small amounts of target dna

Publications (1)

Publication Number Publication Date
DK2992114T3 true DK2992114T3 (da) 2019-06-24

Family

ID=51867654

Family Applications (1)

Application Number Title Priority Date Filing Date
DK14794007.6T DK2992114T3 (da) 2013-05-04 2014-05-02 Berigelse af DNA-sekvensering biblioteker fra prøver indeholdende små mængder af mål-DNA

Country Status (5)

Country Link
US (2) US10576446B2 (da)
EP (1) EP2992114B1 (da)
CN (1) CN105358714B (da)
DK (1) DK2992114T3 (da)
WO (1) WO2014182574A1 (da)

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* Cited by examiner, † Cited by third party
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US9670485B2 (en) 2014-02-15 2017-06-06 The Board Of Trustees Of The Leland Stanford Junior University Partitioning of DNA sequencing libraries into host and microbial components
DK3234200T3 (da) 2014-12-20 2021-08-23 Arc Bio Llc Fremgangsmåde til målrettet depletering af nukleinsyrer ved anvendelse af CRISPR/CAS-systemproteiner
CN105332063B (zh) * 2015-08-13 2017-04-12 厦门飞朔生物技术有限公司 一种单管高通量测序文库的构建方法
AU2016308283B2 (en) 2015-08-19 2022-04-21 Arc Bio, Llc Capture of nucleic acids using a nucleic acid-guided nuclease-based system
CN105844116B (zh) * 2016-03-18 2018-02-27 广州市锐博生物科技有限公司 测序数据的处理方法和处理装置
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
US11999948B2 (en) * 2017-05-04 2024-06-04 The Regents Of The University Of California Methods of producing size-selected nucleic acid libraries and compositions and kits for practicing same
CN109402241A (zh) * 2017-08-07 2019-03-01 深圳华大基因研究院 鉴定和分析古dna样本的方法
WO2019217486A1 (en) * 2018-05-08 2019-11-14 Memorial Sloan Kettering Cancer Center Methods and compositions for detecting myeloma
WO2020033394A1 (en) * 2018-08-06 2020-02-13 Northwestern University Enhanced capture of target nucleic acids
CN111455021B (zh) * 2019-01-18 2024-06-04 广州微远医疗器械有限公司 去除宏基因组中宿主dna的方法及试剂盒
WO2020168286A1 (en) * 2019-02-14 2020-08-20 University Of Washington Systems and methods for improved nanopore-based analysis of nucleic acids
US20220119798A1 (en) * 2019-03-05 2022-04-21 Siemens Healthcare Diagnostics Inc. Compositions and methods for nucleic acid capture by chemical bait
CN111518879B (zh) * 2020-04-24 2021-02-12 上海茂槿生物技术有限公司 一种提高多重pcr扩增文库质量的方法
CN113774052A (zh) * 2021-09-30 2021-12-10 首都医科大学附属北京安贞医院 mtDNA的提取和建库试剂
CN114395554B (zh) * 2022-02-18 2023-03-24 中国林业科学研究院木材工业研究所 一种木质文物的古dna捕获方法
CN116926166A (zh) * 2023-08-23 2023-10-24 北京齐碳科技有限公司 一种长片段dna捕获方法、试剂盒及其用途

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US5766891A (en) 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
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Also Published As

Publication number Publication date
CN105358714B (zh) 2020-03-24
US10981137B2 (en) 2021-04-20
EP2992114A1 (en) 2016-03-09
US20150360194A1 (en) 2015-12-17
US20200139335A1 (en) 2020-05-07
CN105358714A (zh) 2016-02-24
US10576446B2 (en) 2020-03-03
WO2014182574A1 (en) 2014-11-13
EP2992114B1 (en) 2019-04-17
EP2992114A4 (en) 2017-01-04

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