[go: up one dir, main page]

DK2872629T3 - Tm-forstærkede blokeringsoligonukleotider og lokkemidler til forbedret target-berigesle og reduceret off-target-udvælgelse - Google Patents

Tm-forstærkede blokeringsoligonukleotider og lokkemidler til forbedret target-berigesle og reduceret off-target-udvælgelse Download PDF

Info

Publication number
DK2872629T3
DK2872629T3 DK13737778T DK13737778T DK2872629T3 DK 2872629 T3 DK2872629 T3 DK 2872629T3 DK 13737778 T DK13737778 T DK 13737778T DK 13737778 T DK13737778 T DK 13737778T DK 2872629 T3 DK2872629 T3 DK 2872629T3
Authority
DK
Denmark
Prior art keywords
target
lurgets
oigonucleotides
enhanced
reduced
Prior art date
Application number
DK13737778T
Other languages
English (en)
Inventor
Mark Aaron Behlke
John Robert Havens
Scott Daniel Rose
Zachary Zwirko
Doron Lipson
Frank Soo Juhn
Mirna Jarosz
Original Assignee
Integrated Dna Tech Inc
Found Medicine Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Integrated Dna Tech Inc, Found Medicine Inc filed Critical Integrated Dna Tech Inc
Application granted granted Critical
Publication of DK2872629T3 publication Critical patent/DK2872629T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
DK13737778T 2012-07-03 2013-07-03 Tm-forstærkede blokeringsoligonukleotider og lokkemidler til forbedret target-berigesle og reduceret off-target-udvælgelse DK2872629T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261667919P 2012-07-03 2012-07-03
US201261745435P 2012-12-21 2012-12-21
PCT/US2013/049402 WO2014008447A1 (en) 2012-07-03 2013-07-03 Tm-enhanced blocking oligonucleotides and baits for improved target enrichment and reduced off-target selection

Publications (1)

Publication Number Publication Date
DK2872629T3 true DK2872629T3 (da) 2019-12-09

Family

ID=48794242

Family Applications (1)

Application Number Title Priority Date Filing Date
DK13737778T DK2872629T3 (da) 2012-07-03 2013-07-03 Tm-forstærkede blokeringsoligonukleotider og lokkemidler til forbedret target-berigesle og reduceret off-target-udvælgelse

Country Status (9)

Country Link
US (4) US10266889B2 (da)
EP (1) EP2872629B1 (da)
JP (4) JP6433893B2 (da)
AU (1) AU2013286635B2 (da)
CA (1) CA2877740A1 (da)
DK (1) DK2872629T3 (da)
ES (1) ES2769796T3 (da)
SG (2) SG11201408807YA (da)
WO (1) WO2014008447A1 (da)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6054303B2 (ja) 2010-12-30 2016-12-27 ファウンデーション メディシン インコーポレイテッドFoundation Medicine, Inc. 腫瘍試料の多重遺伝子分析の最適化
US9074204B2 (en) * 2011-05-20 2015-07-07 Fluidigm Corporation Nucleic acid encoding reactions
CA2867293C (en) * 2012-03-13 2020-09-01 Abhijit Ajit PATEL Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
AU2013286635B2 (en) * 2012-07-03 2018-11-08 Foundation Medicine, Inc. Tm-enhanced blocking oligonucleotides and baits for improved target enrichment and reduced off-target selection
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US9932576B2 (en) 2012-12-10 2018-04-03 Resolution Bioscience, Inc. Methods for targeted genomic analysis
EP4610368A3 (en) 2013-08-05 2025-11-05 Twist Bioscience Corporation De novo synthesized gene libraries
WO2015105993A1 (en) * 2014-01-09 2015-07-16 AgBiome, Inc. High throughput discovery of new genes from complex mixtures of environmental microbes
US9587268B2 (en) * 2014-01-29 2017-03-07 Agilent Technologies Inc. Fast hybridization for next generation sequencing target enrichment
US9670485B2 (en) * 2014-02-15 2017-06-06 The Board Of Trustees Of The Leland Stanford Junior University Partitioning of DNA sequencing libraries into host and microbial components
EP3158066B1 (en) 2014-06-23 2021-05-12 The General Hospital Corporation Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq)
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
US10648103B2 (en) 2014-10-10 2020-05-12 Invitae Corporation Universal blocking oligo system and improved hybridization capture methods for multiplexed capture reactions
EP4112738B1 (en) * 2014-12-05 2024-07-24 Foundation Medicine, Inc. Multigene analysis of tumor samples
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
EP3235010A4 (en) 2014-12-18 2018-08-29 Agilome, Inc. Chemically-sensitive field effect transistor
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
US10329605B2 (en) 2015-04-20 2019-06-25 Neogenomics Laboratories, Inc. Method to increase sensitivity of detection of low-occurrence mutations
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
MX2017015210A (es) * 2015-05-27 2018-04-13 Quest Diagnostics Invest Inc Composiciones y metodos para el cribado de tumores solidos.
KR101858344B1 (ko) * 2015-06-01 2018-05-16 연세대학교 산학협력단 바코드 서열을 포함하는 어댑터를 이용한 차세대 염기서열 분석 방법
WO2017044609A1 (en) 2015-09-08 2017-03-16 Cold Spring Harbor Laboratory Genetic copy number determination using high throughput multiplex sequencing of smashed nucleotides
JP6982362B2 (ja) 2015-09-18 2021-12-17 ツイスト バイオサイエンス コーポレーション オリゴ核酸変異体ライブラリーとその合成
KR20250053972A (ko) 2015-09-22 2025-04-22 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
US10577643B2 (en) * 2015-10-07 2020-03-03 Illumina, Inc. Off-target capture reduction in sequencing techniques
AU2016353133B2 (en) 2015-11-11 2022-12-08 Resolution Bioscience, Inc. High efficiency construction of dna libraries
WO2017095958A1 (en) 2015-12-01 2017-06-08 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
WO2017106777A1 (en) 2015-12-16 2017-06-22 Fluidigm Corporation High-level multiplex amplification
DK3436607T5 (da) * 2016-03-28 2024-09-16 Ncan Genomics Inc Indfangning af koblede dupleksmål
US11235972B2 (en) * 2016-04-11 2022-02-01 President And Fellows Of Harvard College Sequence design for efficient assembly of nucleic acid structures
CA3020814A1 (en) * 2016-04-15 2017-10-19 University Health Network Hybrid-capture sequencing for determining immune cell clonality
US10811539B2 (en) 2016-05-16 2020-10-20 Nanomedical Diagnostics, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
CN109996876A (zh) 2016-08-22 2019-07-09 特韦斯特生物科学公司 从头合成的核酸文库
CN117286217A (zh) 2016-08-25 2023-12-26 分析生物科学有限公司 用于检测dna样品中基因组拷贝变化的方法
EP4353830A3 (en) 2016-09-15 2024-07-03 ArcherDX, LLC Methods of nucleic acid sample preparation for analysis of cell-free dna
US10704082B2 (en) 2016-09-15 2020-07-07 ArcherDX, Inc. Methods of nucleic acid sample preparation
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
EP4198140B1 (en) * 2016-11-02 2024-09-04 ArcherDX, LLC Methods of nucleic acid sample preparation for immune repertoire sequencing
JP7169975B2 (ja) 2016-12-16 2022-11-11 ツイスト バイオサイエンス コーポレーション 免疫シナプスの変異体ライブラリーおよびその合成
EP3559255A1 (en) 2016-12-23 2019-10-30 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
EP3586255B1 (en) 2017-02-22 2025-01-15 Twist Bioscience Corporation Nucleic acid based data storage
EP3595674A4 (en) 2017-03-15 2020-12-16 Twist Bioscience Corporation BANKS OF VARIANTS OF IMMUNOLOGICAL SYNAPSE AND THEIR SYNTHESIS
WO2018183918A1 (en) * 2017-03-30 2018-10-04 Grail, Inc. Enhanced ligation in sequencing library preparation
US11118222B2 (en) 2017-03-31 2021-09-14 Grail, Inc. Higher target capture efficiency using probe extension
US12473587B2 (en) * 2017-04-06 2025-11-18 Complete Omics Inc. Nucleic acid capture method
US11053537B2 (en) * 2017-05-25 2021-07-06 Integrated Dna Technologies, Inc. Reverse complement adapters for the mitigation of UMI hopping
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
AU2018284227B2 (en) 2017-06-12 2024-05-02 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
JP2020536504A (ja) 2017-09-11 2020-12-17 ツイスト バイオサイエンス コーポレーション Gpcr結合タンパク質およびその合成
EP4026915B1 (en) * 2017-09-28 2023-10-25 Grail, LLC Enrichment of short nucleic acid fragments in sequencing library preparation
WO2019075197A1 (en) 2017-10-11 2019-04-18 The General Hospital Corporation METHODS OF DETECTION OF INDIVIDUAL SITE-SPECIFIC PARASITE GENOMIC DEAMINATION BY BASE EDITING TECHNOLOGIES
AU2018351163A1 (en) * 2017-10-18 2020-05-21 Day Zero Diagnostics, Inc. Selective enrichment of a population of DNA in a mixed DNA sample through targeted suppression of DNA amplification
US10894242B2 (en) 2017-10-20 2021-01-19 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
CN120485344A (zh) 2018-01-04 2025-08-15 特韦斯特生物科学公司 基于dna的数字信息存储
CN112313241B (zh) 2018-04-17 2025-04-15 总医院公司 核酸结合、修饰、和切割试剂的底物偏好和位点的灵敏体外试验
JP2021526366A (ja) * 2018-05-18 2021-10-07 ツイスト バイオサイエンス コーポレーション 核酸ハイブリダイゼーションのためのポリヌクレオチド、試薬、および方法
SG11202011696TA (en) 2018-06-11 2020-12-30 Foundation Medicine Inc Compositions and methods for evaluating genomic alterations
CN108949941A (zh) * 2018-06-25 2018-12-07 北京莲和医学检验所有限公司 低频突变检测方法、试剂盒和装置
WO2020033394A1 (en) * 2018-08-06 2020-02-13 Northwestern University Enhanced capture of target nucleic acids
JP7431802B2 (ja) 2018-08-15 2024-02-15 イルミナ インコーポレイテッド ライブラリー富化を改善するための組成物および方法
WO2020139871A1 (en) 2018-12-26 2020-07-02 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
CN110699426B (zh) * 2019-01-02 2022-01-28 上海臻迪基因科技有限公司 基因目标区域富集方法及试剂盒
CN113728100A (zh) * 2019-02-25 2021-11-30 特韦斯特生物科学公司 用于下一代测序的组合物和方法
WO2020176680A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
SG11202109322TA (en) 2019-02-26 2021-09-29 Twist Bioscience Corp Variant nucleic acid libraries for glp1 receptor
CA3144644A1 (en) 2019-06-21 2020-12-24 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
CN110409001B (zh) * 2019-07-25 2022-11-15 北京贝瑞和康生物技术有限公司 一种构建捕获文库的方法和试剂盒
CN110527715A (zh) * 2019-09-16 2019-12-03 中国科学院遗传与发育生物学研究所农业资源研究中心 一种功能基因组克隆子库的测序方法
CA3155630A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for single domain antibodies
AU2020356471A1 (en) 2019-09-23 2022-04-21 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system
US12134797B2 (en) * 2020-03-26 2024-11-05 Integrated Dna Technologies, Inc. Hybridization capture methods and compositions
MX2023005748A (es) * 2020-11-19 2023-05-26 Regeneron Pharma Genotipado mediante secuenciacion.
US12378596B2 (en) * 2020-12-03 2025-08-05 Roche Sequencing Solutions, Inc. Whole transcriptome analysis in single cells
CN117730155A (zh) * 2021-05-24 2024-03-19 豪夫迈·罗氏有限公司 用于核酸杂交的增强子寡核苷酸
CN118202047A (zh) * 2021-07-06 2024-06-14 斯威齐治疗公司 在小干扰rna链中放置锁核酸的方法
WO2023034969A1 (en) 2021-09-03 2023-03-09 Idbydna Inc. Hybridization probes containing fluorinated carbon chains and related methods

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6110676A (en) * 1996-12-04 2000-08-29 Boston Probes, Inc. Methods for suppressing the binding of detectable probes to non-target sequences in hybridization assays
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
US6936443B2 (en) * 2000-04-03 2005-08-30 Cytyc Corporation Detection and typing of human papillomavirus using PNA probes
JP3706942B2 (ja) * 2001-08-07 2005-10-19 学校法人慶應義塾 物質と蛋白質との間の相互作用の検出方法、物質と相互作用する蛋白質のスクリーニング方法、及び、物質とその物質と相互作用する蛋白質との複合体の形成方法
FR2842534B1 (fr) 2002-07-19 2006-01-20 Inst Nat Sante Rech Med Amplification multiplex quantitative a l'echelle d'un genome, et applications a la detection de remaniements genomiques
US20040022764A1 (en) * 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
US20050181394A1 (en) * 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
EP1660674B1 (en) * 2003-09-10 2010-03-17 Althea Technologies, Inc. Expression profiling using microarrays
US7407753B2 (en) * 2004-05-24 2008-08-05 Yissum Research Development Company Of The Hebrewuniversityofjerusalem Methods, kits and pharmaceutical compositions for diagnosing, delaying onset of, preventing and/or treating osteoporosis
US7867703B2 (en) 2004-08-26 2011-01-11 Agilent Technologies, Inc. Element defined sequence complexity reduction
US20070111960A1 (en) * 2005-03-04 2007-05-17 Advandx, Inc. High affinity probes for analysis of human papillomavirus expression
EP1896617B1 (en) * 2005-05-31 2013-01-02 Life Technologies Corporation Multiplex amplification of short nucleic acids
US20090068643A1 (en) * 2005-11-23 2009-03-12 Integrated Dna Technologies, Inc. Dual Function Primers for Amplifying DNA and Methods of Use
US20100009355A1 (en) * 2006-03-14 2010-01-14 Kolodney Michael S Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides
WO2008143972A2 (en) * 2007-05-18 2008-11-27 Advandx, Inc. Detection of methicillin-resistant staphylococcus aureus
EP2245198A1 (en) 2008-02-04 2010-11-03 Massachusetts Institute of Technology Selection of nucleic acids by solution hybridization to oligonucleotide baits
US20110287424A1 (en) 2009-03-27 2011-11-24 Life Technologies Corporation Methylation-specific competitive allele-specific taqman polymerase chain reaction (cast-pcr)
ES2555389T3 (es) * 2009-03-30 2015-12-30 Illumina, Inc. Análisis de expresión génica en células individuales
EP2270203A1 (en) 2009-06-29 2011-01-05 AIT Austrian Institute of Technology GmbH Oligonucleotide hybridization method
ES2645754T3 (es) 2009-07-30 2017-12-07 F. Hoffmann-La Roche Ag Conjunto de sondas de oligonucleótidos así como métodos y usos relacionados con el mismo
US20110091939A1 (en) * 2009-10-20 2011-04-21 Longze Cui Methods and Compositions for Removing Specific Target Nucleic Acids
US20110129832A1 (en) * 2009-10-27 2011-06-02 Swift Biosciences, Inc. Polynucleotide Primers and Probes
WO2011057119A1 (en) * 2009-11-06 2011-05-12 Enzymatics, Inc. Composition and method for synthesizing a deoxyribonucleotide chain using a double stranded nucleic acid complex with a thermostable polymerase
US20110117559A1 (en) * 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
US8278049B2 (en) * 2010-04-26 2012-10-02 Ann & Robert H. Lurie Children's Hospital of Chicago Selective enrichment of CpG islands
CN101921874B (zh) * 2010-06-30 2013-09-11 深圳华大基因科技有限公司 基于Solexa测序法的检测人类乳头瘤病毒的方法
WO2012018697A1 (en) 2010-08-02 2012-02-09 Integrated Dna Technologies, Inc. Methods for predicting stability and melting temperatures of nucleic acid duplexes
CN101967476B (zh) * 2010-09-21 2012-11-14 深圳华大基因科技有限公司 一种基于接头连接的DNA PCR-Free标签文库构建方法
CN103328654B (zh) * 2010-10-27 2017-07-11 哈佛学院院长等 立足点引物双链体的组合物及其使用方法
WO2012061600A1 (en) 2010-11-05 2012-05-10 The Broad Institute, Inc. Hybrid selection using genome-wide baits for selective genome enrichment in mixed samples
JP6054303B2 (ja) 2010-12-30 2016-12-27 ファウンデーション メディシン インコーポレイテッドFoundation Medicine, Inc. 腫瘍試料の多重遺伝子分析の最適化
US10196681B2 (en) * 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
AU2013286635B2 (en) 2012-07-03 2018-11-08 Foundation Medicine, Inc. Tm-enhanced blocking oligonucleotides and baits for improved target enrichment and reduced off-target selection

Also Published As

Publication number Publication date
JP7459010B2 (ja) 2024-04-01
US11566283B2 (en) 2023-01-31
US20230357841A1 (en) 2023-11-09
ES2769796T3 (es) 2020-06-29
JP2019037248A (ja) 2019-03-14
JP6433893B2 (ja) 2018-12-05
AU2013286635B2 (en) 2018-11-08
US10266889B2 (en) 2019-04-23
CA2877740A1 (en) 2014-01-09
US11566282B2 (en) 2023-01-31
JP2021104031A (ja) 2021-07-26
EP2872629A1 (en) 2015-05-20
AU2013286635A1 (en) 2015-01-22
JP2015521853A (ja) 2015-08-03
JP2021104032A (ja) 2021-07-26
EP2872629B1 (en) 2019-09-04
SG11201408807YA (en) 2015-01-29
US20140031240A1 (en) 2014-01-30
SG10201610861XA (en) 2017-02-27
JP7358413B2 (ja) 2023-10-10
US20170096706A1 (en) 2017-04-06
US20170114404A1 (en) 2017-04-27
WO2014008447A1 (en) 2014-01-09

Similar Documents

Publication Publication Date Title
DK2872629T3 (da) Tm-forstærkede blokeringsoligonukleotider og lokkemidler til forbedret target-berigesle og reduceret off-target-udvælgelse
DK2854883T3 (da) Biomatrix-hydrogeler og fremgangsmåder til anvendelse deraf
DK3468197T3 (da) Fremgangsmåde til interforudsigelse og indretning dertil
DK2925782T3 (da) Anti-cd47-antistoffer og fremgangsmåder til anvendelse deraf
DK2852680T3 (da) Fremgangsmåder og processer til ikke-invasiv evaluering af genetiske variationer
DK3196022T3 (da) Trykningssystem og fremgangsmåde
DK3421051T3 (da) Fremgangsmåder til glycokonjugering og sammensætninger
DK2573542T3 (da) Automatiseret farvningssystem og reaktionskammer
DK2855736T3 (da) Elektrolysator og energisystem
DK2906684T3 (da) T-celle-modificerende forbindelser og anvendelser deraf
DK2920167T3 (da) Hydrazinyl-indol-forbindelser og konjugater
DK2850202T3 (da) Fremgangsmåder og grupper
DK3045187T3 (da) Injektor og fremgangsmåde til samling
DK3597644T3 (da) Kationiske aminlipider og anvendelser deraf
DK2857401T5 (da) HIDTIL UKENDT ß-LACTAMASEINHIBITOR OG FREMGANGSMÅDE TIL AT FREMSTILLE SAMME
DK2812443T3 (da) Cd47-antistoffer og fremgangsmåder til anvendelse deraf
DK2692026T3 (da) Forspændingselement til fastholdelse af kontinuitet og tilknyttet fremgangsmåde
DK2699397T3 (da) Apparater, system og fremgangsmåder til at danne pressede artikler og pressede artikler dannet derved
DK2890780T3 (da) Fremgangsmåder og sammensætninger til behandling af en genetisk tilstand
SG11201406717RA (en) Nucleic acid amplification
DK2758349T3 (da) Vanadiumbaserede frittematerialer og fremgangsmåder til fremstilling heraf
DK3381461T3 (da) Peptider og fremgangsmåder til anvendelse deraf
DK2908661T3 (da) Mikroalgemelgranulater og fremgangsmåde til fremstilling deraf
DK2917181T3 (da) Heteroarylderivater og anvendelser deraf
DK2858196T3 (da) System, fremgangsmåde og program til energibesparelse