DK2638398T3 - Hidtil ukendt markør til påvisning af blærecancer og/eller inflammatoriske tilstande i blæren. - Google Patents
Hidtil ukendt markør til påvisning af blærecancer og/eller inflammatoriske tilstande i blæren. Download PDFInfo
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- DK2638398T3 DK2638398T3 DK11846559.0T DK11846559T DK2638398T3 DK 2638398 T3 DK2638398 T3 DK 2638398T3 DK 11846559 T DK11846559 T DK 11846559T DK 2638398 T3 DK2638398 T3 DK 2638398T3
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Claims (10)
1. Fremgangsmåde til påvisning af blærecancer omfattende: (i) tilvejebringelse af en urinprøve; (ii) påvisning af niveauerne af det humane neutrofilmarkørgen interleukin 8 receptor B (IL8Rb) i tilknytning til blærecancermarkør (BTM)-gener i prøven, hvor BTM-generne omfatter celledelingscyklus 2, GI til S og G2 til M (CDC2), midkine (neuritvækstfrem-mende faktor 2) (MDK), Homeo Box A13 (HOXA13) og insulin-lignende vækstfaktorbindende protein 5 (IGFBP5); og (iii) hvori tilstedeværelse af cancer fastlægges ved sammenligning af niveauerne af IL8Rb og BTM-generne med niveauerne hos normale patienter og/eller patienter med blærecancer og/eller patienter med en inflammatorisk blæretilstand og fastlæggelse af, om patienten har blærecancer, hvori en øgning i niveauerne af BTM-generne uden en øgning i niveauet af IL8Rb indikerer, at patienten har blærecancer, og hvori en øgning i niveauet af IL8Rb indikerer, at patienten haren inflammatorisk blæretilstand.
2. Fremgangsmåde ifølge krav 1, hvori: (a) sandsynligheden for tilstedeværelse af cancer bestemmes ved fastlæggelse af en diagnostisk tærskelværdi baseret på de kendte ekspressionsniveauer af IL8Rb og BTM-generne med niveauerne hos normale patienter og/eller patienter med blærecancer og/eller patienter med en inflammatorisk blæretilstand, eventuelt hvori tærskelværdien fastlægges ved anvendelse af en statistisk metode, og eventuelt hvori den statistiske metode er en hvilken som helst af lineær diskriminantanalyse (LDA), logistisk regression (LogReg), Support Vector Machine (SVM), K-nearest 5 neighbors (KN5N) og Partition Tree Classifier (TREE); og/eller (b) BTM-generne inkluderer mindst et yderligere markørgen udvalgt fra figur 6 eller figur 7.
3. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvori: (a) trinnet med påvisning udføres ved påvisning af niveauerne af mRNA; eller (b) trinnet med påvisning udføres ved påvisning af niveauerne af cDNA, eventuelt ved anvendelse af et oligonukleotid, der er komplementært til mindst en andel af cDNA'et, eller ved anvendelse af qRT-PCR-fremgangsmåde ved anvendelse af en forward-primer og en revers primer.
4. Anvendelse af en anordning til påvisning af en blærecancer i en urinprøve, hvilken anordning omfatter: et substrat med et IL8Rb-gen-capture-reagens og et capture-reagens for blære-tumormarkør (BTM)-gener derpå, hvori BTM-generne omfatter celledelingscyklus 2, GI til S og G2 til M (CDC2), midkine (neuritvækstfremmende faktor 2) (MDK), Homeo Box A13 (HOXA13) og insulin-lignende vækstfaktor-bindende protein 5 (IGFBP5); og en detektor i tilknytning til substratet, hvilken detektor er i stand til at påvise ek spressionen i tilknytning til capture-reagenset, hvori en øgning i niveauerne af BTM-generne uden en øgning i niveauet af IL8Rb indikerer, at patienten har blærecancer, og hvori en øgning i niveauet af IL8Rb indikerer, at patienten haren inflammatorisk blæretilstand.
5. Anvendelse ifølge krav 4, hvori BTM-generne inkluderer mindst en yderligere markør udvalgt fra figur 6 eller figur 7.
6. Anvendelse ifølge et hvilket som helst af kravene 4 til 5, hvori: (a) capture-reagenset er et oligonukleotid; eller (b) capture-reagenset er et antistof, der er specifikt for enten et oligonukleotid, et protein eller et peptid.
7. Anvendelse af et kit til påvisning af blærecancer i en urinprøve, hvilket kit omfatter: et substrat med et IL8Rb-gen-capture-reagens og et capture-reagens for blære-tumormarkør (BTM)-gener derpå, hvori BTM omfatter celledelingscyklus 2, GI til S og G2 til M (CDC2), midkine (neuritvækstfremmende faktor 2) (MDK), Homeo Box A13 (HOXA13) og insulin-lignende vækstfaktor-bindende protein 5 (IGFBP5); et middel til visualisering af et kompleks af capture-midlet og en markør; reagenser og en brugsanvisning, hvori en øgning i niveauerne af BTM-generne uden en øgning i niveauet af IL8Rb indikerer, at patienten har blærecancer, og hvori en øgning i niveauet af IL8Rb indikerer, at patienten haren inflammatorisk blæretilstand.
8. Anvendelse ifølge krav 7, hvori: (a) capture-reagenset er et oligonukleotid; eller (b) capture-reagenset er et antistof, der er selektivt for et oligonukleotid, et protein eller et peptid.
9. Fremgangsmåde til screening for blærecancer omfattende trinnene med: tilvejebringelse af en testurinprøve fra et testsubjekt; måling af tilstedeværelse af IL8Rb-genet og blæretumormarkør (BTM)-generne i testurinprøven, hvori BTM-generne omfatter celledelingscyklus 2, GI til S og G2 til M (CDC2), midkine (neuritvækstfremmende faktor 2) (MDK), Homeo Box A13 (HOXA13) og insulin-lignende vækstfaktor-bindende protein 5 (IGFBP5); og sammenligning af mængden af markører, der er til stede i testurinprøven med en værdi opnået fra en kontrolurinprøve fra et subjekt, der ikke har blærecancer, og/eller med en kontrolprøve med et subjekt, der har en inflammatorisk tilstand i blæren, og fastlæggelse af, om der er blærecancer til stede, hvori en øgning i niveauerne af BTM-generne uden en øgning i niveauet af IL8Rb indikerer tilstedeværelse af blærecancer, og hvori en øgning i niveauet af IL8Rb indikerer tilstedeværelse af en inflammatorisk blæretilstand.
10. Fremgangsmåde ifølge krav 9, hvori: (a) den påviste markør er et oligonukleotid, eventuelt DNA eller RNA; og/eller (c) trinnet med måling anvender et ELISA-assay.
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| PCT/NZ2011/000238 WO2012078053A1 (en) | 2010-11-12 | 2011-11-11 | Novel marker for detection of bladder cancer and/or inflammatory conditions of the bladder |
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| WO2015073870A1 (en) * | 2013-11-15 | 2015-05-21 | North Carolina State University | Chromosomal assessment to diagnose urogenital malignancy in dogs |
| PT3071973T (pt) * | 2013-11-21 | 2021-01-13 | Pacific Edge Ltd | Triagem de pacientes com hematúria assintomática usando biomarcadores genotípicos e fenotípicos |
| WO2015160916A1 (en) | 2014-04-15 | 2015-10-22 | North Carolina State University | Chromosomal assessment to differentiate histiocytic malignancy from lymphoma and hemangiosarcoma in dogs |
| WO2016014941A1 (en) | 2014-07-24 | 2016-01-28 | North Carolina State University | Method to diagnose malignant melanoma in the domestic dog |
| FR3044613B1 (fr) * | 2015-12-08 | 2018-11-30 | Faurecia Interieur Industrie | Equipement interieur de vehicule equipe d'un ecran, procede de gestion correspondant |
| WO2017210115A1 (en) | 2016-05-31 | 2017-12-07 | North Carolina State University | Methods of mast cell tumor prognosis and uses thereof |
| CN106520949B (zh) * | 2016-11-14 | 2019-05-14 | 北京开景基因技术有限公司 | 生物标记用于制备腺性膀胱炎诊断试剂的用途 |
| CN106709267A (zh) * | 2017-01-25 | 2017-05-24 | 武汉贝纳科技服务有限公司 | 数据获取方法及装置 |
| EP3450981A1 (en) * | 2017-08-28 | 2019-03-06 | Trizell GmbH | Btnl8 as a marker for tregs |
| CN108866194B (zh) * | 2018-08-16 | 2021-03-26 | 杭州可帮基因科技有限公司 | 一组用于膀胱癌检测的基因及其应用 |
| CN109055559A (zh) * | 2018-09-28 | 2018-12-21 | 爱尔生基因医学科技有限公司 | 一种检测膀胱癌的特异性引物和试剂盒及其检测方法 |
| CA3275342A1 (en) * | 2022-12-02 | 2024-06-06 | Novelna Inc. | Pan-cancer and tissue-specific cancer-causing proteins and their methods of use |
| CN116024345B (zh) * | 2023-01-12 | 2023-12-01 | 上海仁度生物科技股份有限公司 | 膀胱癌的实时荧光核酸恒温扩增检测试剂盒及其专用引物和探针 |
| CN118443941A (zh) * | 2024-04-29 | 2024-08-06 | 山东大学齐鲁医院 | 中性粒细胞作为诊疗膀胱癌的靶标的应用 |
| CN119979716B (zh) * | 2025-02-19 | 2025-10-17 | 西北农林科技大学 | 转录因子hoxa13在作为禽类腹脂率检测标志物中的应用 |
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| WO2000052204A2 (en) * | 1999-02-22 | 2000-09-08 | Orntoft Torben F | Gene expression in bladder tumors |
| WO2001020333A1 (en) * | 1999-09-10 | 2001-03-22 | Meiji Milk Prod Co Ltd | Early cancer tumor marker |
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| US20040076955A1 (en) * | 2001-07-03 | 2004-04-22 | Eos Biotechnology, Inc. | Methods of diagnosis of bladder cancer, compositions and methods of screening for modulators of bladder cancer |
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| US7256707B2 (en) | 2004-06-18 | 2007-08-14 | Los Alamos National Security, Llc | RF transmission line and drill/pipe string switching technology for down-hole telemetry |
| TWI585411B (zh) * | 2004-07-23 | 2017-06-01 | 太平洋愛吉生技股份有限公司 | 檢測膀胱癌之尿液標記物 |
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| CN100366637C (zh) * | 2005-11-17 | 2008-02-06 | 中国人民解放军第四军医大学 | 人白细胞介素8拮抗蛋白及其制备方法 |
| NZ545243A (en) * | 2006-02-10 | 2009-07-31 | Pacific Edge Biotechnology Ltd | Urine gene expression ratios for detection of cancer |
| CN101101290A (zh) * | 2006-11-30 | 2008-01-09 | 山东省医药生物技术研究中心 | 肿瘤血清标志物及其应用 |
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| CN101768214B (zh) * | 2008-10-06 | 2013-03-13 | 复旦大学 | 一种人肿瘤标志物-Tim17多肽及其用途 |
| WO2010059742A1 (en) | 2008-11-18 | 2010-05-27 | Collabrx, Inc. | Individualized cancer treatment |
| US20100166739A1 (en) * | 2008-12-30 | 2010-07-01 | Lipella Paharmaceuticals Inc. | Methods and Compositions for Diagnosing Urological Disorders |
| PT3071973T (pt) * | 2013-11-21 | 2021-01-13 | Pacific Edge Ltd | Triagem de pacientes com hematúria assintomática usando biomarcadores genotípicos e fenotípicos |
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| CN103314298B (zh) | 2016-05-25 |
| WO2012078053A1 (en) | 2012-06-14 |
| NO2638398T3 (da) | 2018-06-23 |
| SG10201509322YA (en) | 2015-12-30 |
| HK1189660A1 (zh) | 2014-06-13 |
| AU2017202630A1 (en) | 2017-05-18 |
| AU2011339073A1 (en) | 2013-06-20 |
| JP5985493B2 (ja) | 2016-09-06 |
| EP2638398A1 (en) | 2013-09-18 |
| KR20140040070A (ko) | 2014-04-02 |
| SG190247A1 (en) | 2013-07-31 |
| PT2638398T (pt) | 2018-06-01 |
| US11208698B2 (en) | 2021-12-28 |
| EP2638398A4 (en) | 2014-03-26 |
| BR112013011875A2 (pt) | 2021-04-13 |
| ES2667029T3 (es) | 2018-05-09 |
| TWI618797B (zh) | 2018-03-21 |
| WO2012078053A8 (en) | 2013-05-30 |
| CA2817701A1 (en) | 2012-06-14 |
| JP2013543974A (ja) | 2013-12-09 |
| KR101974135B1 (ko) | 2019-04-30 |
| CA2817701C (en) | 2021-10-19 |
| US9982305B2 (en) | 2018-05-29 |
| EP2638398B1 (en) | 2018-01-24 |
| TW201305350A (zh) | 2013-02-01 |
| US20170002420A1 (en) | 2017-01-05 |
| US20180298452A1 (en) | 2018-10-18 |
| NZ589251A (en) | 2014-07-25 |
| BR112013011875B1 (pt) | 2022-06-14 |
| CN103314298A (zh) | 2013-09-18 |
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