DK2668268T3 - Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem - Google Patents
Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem Download PDFInfo
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- DK2668268T3 DK2668268T3 DK12702939.5T DK12702939T DK2668268T3 DK 2668268 T3 DK2668268 T3 DK 2668268T3 DK 12702939 T DK12702939 T DK 12702939T DK 2668268 T3 DK2668268 T3 DK 2668268T3
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Claims (15)
1. Isoleret polypeptid med cellobiohydrolaseaktivitet, som er valgt fra gruppen bestående af: (a) et polypeptid, der udviser mindst 90% sekvensidentitet med aminosyrerne 19 til 455 ifølge SEQ ID NO: 2; (b) et polypeptid, der kodes for af et polynukleotid, som udviser mindst 90% sekvensidentitet med nukleotiderne 55 til 603, 668 til 1235, 1311 til 1507 ifølge SEQ ID NO: 1 eller cDNA-sekvensen deraf; og (c) et fragment af polypeptidet ifølge (a) eller (b), der har cellobiohydrolaseaktivitet.
2. Polypeptid ifølge krav 1, som endvidere omfatter et cellulosebindende domæne.
3. Sammensætning, som omfatter polypeptidet ifølge krav 1 eller 2.
4. Isoleret polynukleotid, som koder for polypeptidet ifølge krav 1 eller 2.
5. Nukleinsyrekonstruktion eller ekspressionsvektor, som omfatter polynukleotidet ifølge krav 4, hvilket polynukleotid er operativt forbundet til en eller flere kontrolsekvenser, der styrer fremstillingen af polypeptidet i en ekspressionsvært.
6. Rekombinant værtscelle, som omfatter polynukleotidet ifølge krav 4, hvilket polynukleotid er operativt forbundet til en eller flere kontrolsekvenser, der styrer fremstillingen af polypeptidet.
7. Fremgangsmåde til fremstilling af et polypeptid med cellobiohydrolaseaktivitet, hvilken fremgangsmåde omfatter: (a) dyrkning af den rekombinante værtscelle ifølge krav 6 under betingelser, der er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
8. Fremgangsmåde til nedbrydning eller omdannelse af et celluloseholdigt materiale, hvilken fremgangsmåde omfatter: behandling af det celluloseholdige materiale med en enzymsammensætning i nærværelse af polypeptidet med cellobiohydrolaseaktivitet ifølge krav 1 eller 2.
9. Fremgangsmåde ifølge krav 8, hvor det celluloseholdige materiale forbehandles.
10. Fremgangsmåde ifølge krav 8 eller 9, som endvidere omfatter indvinding af det nedbrudte celluloseholdige materiale.
11. Fremgangsmåde ifølge et hvilket som helst af kravene 8-10, hvor enzymsammensætningen omfatter et eller flere enzymer valgt fra gruppen bestående af en endoglucanase, en yderligere cellobiohydrolase og en beta-glucosidase.
12. Fremgangsmåde til fremstilling af et fermenteringsprodukt, hvilken fremgangsmåde omfatter: (a) forsukring af et celluloseholdigt materiale med en enzymsammensætning i nærværelse af et polypeptid med cellobiohydrolaseaktivitet ifølge krav 1 eller 2; (b) fermentering af det forsukrede celluloseholdige materiale med en eller flere fermenterende mikroorganismer for at fremstille fermenteringsproduktet; og (c) indvinding af fermenteringsproduktet fra fermenteringen.
13. Fremgangsmåde ifølge krav 12, hvor det celluloseholdige materiale forbehandles.
14. Fremgangsmåde ifølge krav 13, hvor fermenteringsproduktet er en alkohol, en organisk syre, en keton, en aminosyre eller en gas.
15. Fremgangsmåde til fermentering af et celluloseholdigt materiale, hvilken fremgangsmåde omfatter: fermentering af det celluloseholdige materiale med en eller flere fermenterende mikroorganismer, hvor det celluloseholdige materiale er forsukret med en enzymsammensætning i nærværelse af et polypeptid med cellobiohydrolaseaktivitet ifølge krav 1 eller 2.
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| US201161483116P | 2011-05-06 | 2011-05-06 | |
| US201161546602P | 2011-10-13 | 2011-10-13 | |
| PCT/US2012/022671 WO2012103300A2 (en) | 2011-01-26 | 2012-01-26 | Polypeptides having cellobiohydrolase activity and polynucleotides encoding same |
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| DK17167880.8T DK3235903T3 (da) | 2011-01-26 | 2012-01-26 | Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem |
| DK12702729.0T DK2668267T3 (da) | 2011-01-26 | 2012-01-26 | Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem |
| DK12700867.0T DK2668265T3 (da) | 2011-01-26 | 2012-01-26 | Hidtil ukendte glycosidhydrolaser fra termofile svampe |
| DK12702939.5T DK2668268T3 (da) | 2011-01-26 | 2012-01-26 | Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem |
| DK12702942.9T DK2668269T3 (da) | 2011-01-26 | 2012-01-26 | Polypeptider med endoglucanaseaktivitet og polynukleotider, som koder for dem |
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| DK17167880.8T DK3235903T3 (da) | 2011-01-26 | 2012-01-26 | Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem |
| DK12702729.0T DK2668267T3 (da) | 2011-01-26 | 2012-01-26 | Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem |
| DK12700867.0T DK2668265T3 (da) | 2011-01-26 | 2012-01-26 | Hidtil ukendte glycosidhydrolaser fra termofile svampe |
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| Application Number | Title | Priority Date | Filing Date |
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| DK12702942.9T DK2668269T3 (da) | 2011-01-26 | 2012-01-26 | Polypeptider med endoglucanaseaktivitet og polynukleotider, som koder for dem |
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| MX337919B (es) | 2011-01-26 | 2016-03-28 | Novozymes As | Polipeptidos que tienen actividad de celobiohidrolasa y polinucleotidos que codifican los mismos. |
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| BR112013019039A2 (pt) | 2011-01-26 | 2020-07-21 | Novozymes A/S | Célula hospedeira microbiana transgênica, métodos para produzir um polipeptídeo, para produzir um mutante de uma célula originária, para produzir uma proteína, para degradar um material celulósico, para produzir um produto de fermentação, 5 e parafermentar um material celulósico, construção de ácido nucleico ou vetor de expressão, polipeptídeo isolado, e polinucleotídeo isolado |
| WO2012103322A1 (en) * | 2011-01-26 | 2012-08-02 | Novozymes A/S | Polypeptides having endoglucanase activity and polynucleotides encoding same |
| US10036050B2 (en) | 2011-12-20 | 2018-07-31 | Novozymes, Inc. | Cellobiohydrolase variants and polynucleotides encoding same |
| ES2935920T3 (es) * | 2012-03-30 | 2023-03-13 | Novozymes North America Inc | Procesos de elaboración de productos de fermentación |
| EP2841567B1 (en) | 2012-04-27 | 2017-07-26 | Novozymes, Inc. | Gh61 polypeptide variants and polynucleotides encoding same |
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| US20150275194A1 (en) | 2012-10-24 | 2015-10-01 | Novozymes A/S | Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same |
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| US8753860B1 (en) * | 2013-02-12 | 2014-06-17 | Novozymes A/S | Polypeptides having cellobiohydrolase activity and polynucleotides encoding same |
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| US8759040B1 (en) * | 2013-02-12 | 2014-06-24 | Novozymes Inc. | Polypeptides having cellobiohydrolase activity and polynucleotides encoding same |
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| WO2014182990A1 (en) | 2013-05-10 | 2014-11-13 | Novozymes A/S | Polypeptides having xylanase activity and polynucleotides encoding same |
| BR112016004818B1 (pt) | 2013-09-04 | 2022-05-31 | Novozymes A/S | Um processo para degradação de um material celulósico, um processo para produção de um produto de fermentação, um processo de fermentação de um material celulósico e uma composição de enzimas |
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| DK3092312T3 (da) | 2014-01-07 | 2019-03-04 | Novozymes As | Forsukring af mannanholdige celluloseholdige materialer |
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| US10513721B2 (en) | 2015-03-12 | 2019-12-24 | Novozymes A/S | Two-stage process for enzymatic hydrolysis of lignocellulosic biomass |
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| WO2011059740A1 (en) | 2009-10-29 | 2011-05-19 | Novozymes, Inc. | Polypeptides having cellobiohydrolase activity and polynucleotides encoding same |
| BR112012011596B1 (pt) | 2009-11-04 | 2018-10-02 | Dsm Ip Assets Bv | transformante de talaromyces, processos para a produção de um transformante de talaromyces, de um transformante múltiplo de talaromyces e de uma composição polipeptídica compreendendo uma ou mais celulases, e processos para a sacarificação de material lignocelulósico e para a preparação de um produto de fermentação |
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| US10035996B2 (en) * | 2012-10-08 | 2018-07-31 | Novozymes, Inc. | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| CN104870639A (zh) * | 2012-12-14 | 2015-08-26 | 诺维信公司 | 具有纤维素分解增强活性的多肽以及编码它们的多核苷酸 |
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