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DK2668268T3 - Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem - Google Patents

Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem Download PDF

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Publication number
DK2668268T3
DK2668268T3 DK12702939.5T DK12702939T DK2668268T3 DK 2668268 T3 DK2668268 T3 DK 2668268T3 DK 12702939 T DK12702939 T DK 12702939T DK 2668268 T3 DK2668268 T3 DK 2668268T3
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Denmark
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polypeptide
seq
vol
cellulosic material
polynucleotide
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DK12702939.5T
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English (en)
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Marc D Morant
Paul Harris
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Novozymes Inc
Novozymes As
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
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    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445Beta-glucosidase (3.2.1.21)
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    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/02Monosaccharides
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    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/14Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/14Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
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    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
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    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
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    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01176Cellulose 1,4-beta-cellobiosidase (reducing end)(3.2.1.176)
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    • C12P2201/00Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (15)

1. Isoleret polypeptid med cellobiohydrolaseaktivitet, som er valgt fra gruppen bestående af: (a) et polypeptid, der udviser mindst 90% sekvensidentitet med aminosyrerne 19 til 455 ifølge SEQ ID NO: 2; (b) et polypeptid, der kodes for af et polynukleotid, som udviser mindst 90% sekvensidentitet med nukleotiderne 55 til 603, 668 til 1235, 1311 til 1507 ifølge SEQ ID NO: 1 eller cDNA-sekvensen deraf; og (c) et fragment af polypeptidet ifølge (a) eller (b), der har cellobiohydrolaseaktivitet.
2. Polypeptid ifølge krav 1, som endvidere omfatter et cellulosebindende domæne.
3. Sammensætning, som omfatter polypeptidet ifølge krav 1 eller 2.
4. Isoleret polynukleotid, som koder for polypeptidet ifølge krav 1 eller 2.
5. Nukleinsyrekonstruktion eller ekspressionsvektor, som omfatter polynukleotidet ifølge krav 4, hvilket polynukleotid er operativt forbundet til en eller flere kontrolsekvenser, der styrer fremstillingen af polypeptidet i en ekspressionsvært.
6. Rekombinant værtscelle, som omfatter polynukleotidet ifølge krav 4, hvilket polynukleotid er operativt forbundet til en eller flere kontrolsekvenser, der styrer fremstillingen af polypeptidet.
7. Fremgangsmåde til fremstilling af et polypeptid med cellobiohydrolaseaktivitet, hvilken fremgangsmåde omfatter: (a) dyrkning af den rekombinante værtscelle ifølge krav 6 under betingelser, der er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
8. Fremgangsmåde til nedbrydning eller omdannelse af et celluloseholdigt materiale, hvilken fremgangsmåde omfatter: behandling af det celluloseholdige materiale med en enzymsammensætning i nærværelse af polypeptidet med cellobiohydrolaseaktivitet ifølge krav 1 eller 2.
9. Fremgangsmåde ifølge krav 8, hvor det celluloseholdige materiale forbehandles.
10. Fremgangsmåde ifølge krav 8 eller 9, som endvidere omfatter indvinding af det nedbrudte celluloseholdige materiale.
11. Fremgangsmåde ifølge et hvilket som helst af kravene 8-10, hvor enzymsammensætningen omfatter et eller flere enzymer valgt fra gruppen bestående af en endoglucanase, en yderligere cellobiohydrolase og en beta-glucosidase.
12. Fremgangsmåde til fremstilling af et fermenteringsprodukt, hvilken fremgangsmåde omfatter: (a) forsukring af et celluloseholdigt materiale med en enzymsammensætning i nærværelse af et polypeptid med cellobiohydrolaseaktivitet ifølge krav 1 eller 2; (b) fermentering af det forsukrede celluloseholdige materiale med en eller flere fermenterende mikroorganismer for at fremstille fermenteringsproduktet; og (c) indvinding af fermenteringsproduktet fra fermenteringen.
13. Fremgangsmåde ifølge krav 12, hvor det celluloseholdige materiale forbehandles.
14. Fremgangsmåde ifølge krav 13, hvor fermenteringsproduktet er en alkohol, en organisk syre, en keton, en aminosyre eller en gas.
15. Fremgangsmåde til fermentering af et celluloseholdigt materiale, hvilken fremgangsmåde omfatter: fermentering af det celluloseholdige materiale med en eller flere fermenterende mikroorganismer, hvor det celluloseholdige materiale er forsukret med en enzymsammensætning i nærværelse af et polypeptid med cellobiohydrolaseaktivitet ifølge krav 1 eller 2.
DK12702939.5T 2011-01-26 2012-01-26 Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem DK2668268T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP11152252 2011-01-26
US201161483116P 2011-05-06 2011-05-06
US201161546602P 2011-10-13 2011-10-13
PCT/US2012/022671 WO2012103300A2 (en) 2011-01-26 2012-01-26 Polypeptides having cellobiohydrolase activity and polynucleotides encoding same

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DK2668268T3 true DK2668268T3 (da) 2017-09-11

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DK17167880.8T DK3235903T3 (da) 2011-01-26 2012-01-26 Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem
DK12702729.0T DK2668267T3 (da) 2011-01-26 2012-01-26 Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem
DK12700867.0T DK2668265T3 (da) 2011-01-26 2012-01-26 Hidtil ukendte glycosidhydrolaser fra termofile svampe
DK12702939.5T DK2668268T3 (da) 2011-01-26 2012-01-26 Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem
DK12702942.9T DK2668269T3 (da) 2011-01-26 2012-01-26 Polypeptider med endoglucanaseaktivitet og polynukleotider, som koder for dem

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DK17167880.8T DK3235903T3 (da) 2011-01-26 2012-01-26 Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem
DK12702729.0T DK2668267T3 (da) 2011-01-26 2012-01-26 Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem
DK12700867.0T DK2668265T3 (da) 2011-01-26 2012-01-26 Hidtil ukendte glycosidhydrolaser fra termofile svampe

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DK12702942.9T DK2668269T3 (da) 2011-01-26 2012-01-26 Polypeptider med endoglucanaseaktivitet og polynukleotider, som koder for dem

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US (9) US9353363B2 (da)
EP (5) EP2668269B1 (da)
CN (4) CN103562384B (da)
BR (2) BR112013019038B1 (da)
CA (1) CA2834023A1 (da)
DK (5) DK3235903T3 (da)
MX (1) MX337985B (da)
WO (2) WO2012101206A2 (da)

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