DK2518162T3 - Multi-tag sekvensering og økogenomisk analyse - Google Patents
Multi-tag sekvensering og økogenomisk analyse Download PDFInfo
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- DK2518162T3 DK2518162T3 DK12161878.9T DK12161878T DK2518162T3 DK 2518162 T3 DK2518162 T3 DK 2518162T3 DK 12161878 T DK12161878 T DK 12161878T DK 2518162 T3 DK2518162 T3 DK 2518162T3
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Claims (4)
1. Kit, som er egnet til forstærkning og multipleks-sekvensering, omfattende: i det mindste 5 par mærkede (taggede) fremad- og tilbagegående primere med mærke med henblik på forstærkning med særskilt anbringelse, hvorved hver fremad- og tilbagegående primer i respektive par omfatter, i 5’ til 3’ orden: en tag-sekvens, hvorved tag-sekvensen i hvert af primer-parrene er forskelligt fra tag-sekvensen for de andre primer-par; og en priming-sekvens, der er specifik for en target-sekvens, hvorved priming-sekvensen i hvert af primer-parrene er den samme; og hvorved enten: tag-sekvensens længde er 4 til 36 nukleotider, og priming-sekvensen er komplementær til en target-sekvens, lokaliseret 3’ til en 16S rRNA-sekvens; eller tag-sekvensens længde er 6 til 36 nukleotider, og priming-sekvensen er komplementær til en target-sekvens, lokaliseret 3’ til en 16S rRNA sekvens, en 18S rRNA-sekvens, en ITS-sekvens, en mitokon-drial sekvens, en mikrostatellit-sekvens, en metabolisk enzymsekvens, eller en genetisk-sygdom-sekvens.
2. Kit ifølge krav 1, omfattende et sæt af fra 10 til 25, eller fra 20 til 50, eller fra 25 til 75, eller fra 50 til 100, eller fra 50 til 150, eller fra 100 til 200, eller fra 250 til 750 eller fra 100 til 1000 taggede primer-par.
3. Kit ifølge krav 1, hvorved tag-sekvensernes længde er 5, 6, 7 eller 8 nukleotider.
4. Kit ifølge krav 1, hvorved primerne ydermere omfatter en gruppering med henblik på immobilisering.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US85894806P | 2006-11-15 | 2006-11-15 | |
| EP07871488.8A EP2082063B1 (en) | 2006-11-15 | 2007-11-15 | Multitag sequencing and ecogenomics analysis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2518162T3 true DK2518162T3 (da) | 2018-06-18 |
Family
ID=39402482
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK12161878.9T DK2518162T3 (da) | 2006-11-15 | 2007-11-15 | Multi-tag sekvensering og økogenomisk analyse |
Country Status (6)
| Country | Link |
|---|---|
| US (4) | US8603749B2 (da) |
| EP (2) | EP2518162B1 (da) |
| CA (2) | CA2669728C (da) |
| DK (1) | DK2518162T3 (da) |
| ES (2) | ES2679996T3 (da) |
| WO (1) | WO2008061193A2 (da) |
Families Citing this family (149)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE364718T1 (de) | 1997-04-01 | 2007-07-15 | Solexa Ltd | Verfahren zur vervielfältigung von nukleinsäure |
| DE602005009324D1 (de) | 2005-04-06 | 2008-10-09 | Maurice Stroun | Methode zur Krebsdiagnose mittels Nachweis von DNA und RNA im Kreislauf |
| WO2008109176A2 (en) | 2007-03-07 | 2008-09-12 | President And Fellows Of Harvard College | Assays and other reactions involving droplets |
| AR066922A1 (es) * | 2007-06-08 | 2009-09-23 | Monsanto Technology Llc | Metodos de mejoramiento molecular del germoplasma de una planta por secuenciamiento dirigido |
| CN101946010B (zh) | 2007-12-21 | 2014-08-20 | 哈佛大学 | 用于核酸测序的系统和方法 |
| CN102203287B (zh) * | 2008-08-26 | 2017-09-19 | 弗卢迪格姆公司 | 增加样本和/或靶通量的测定方法 |
| US9909170B2 (en) | 2008-09-05 | 2018-03-06 | Washington University | Method for multiplexed nucleic acid patch polymerase chain reaction |
| US11111514B2 (en) | 2008-09-05 | 2021-09-07 | Washington University | Method for multiplexed nucleic acid patch polymerase chain reaction |
| US8586310B2 (en) * | 2008-09-05 | 2013-11-19 | Washington University | Method for multiplexed nucleic acid patch polymerase chain reaction |
| EP3290531B1 (en) | 2008-12-19 | 2019-07-24 | President and Fellows of Harvard College | Particle-assisted nucleic acid sequencing |
| JP5457222B2 (ja) | 2009-02-25 | 2014-04-02 | エフ．ホフマン−ラ ロシュ アーゲー | 小型化ハイスループット核酸分析 |
| SG174617A1 (en) | 2009-04-02 | 2011-10-28 | Fluidigm Corp | Multi-primer amplification method for barcoding of target nucleic acids |
| AU2010242073C1 (en) | 2009-04-30 | 2015-12-24 | Good Start Genetics, Inc. | Methods and compositions for evaluating genetic markers |
| US12129514B2 (en) | 2009-04-30 | 2024-10-29 | Molecular Loop Biosolutions, Llc | Methods and compositions for evaluating genetic markers |
| EP3029141A1 (en) | 2009-08-20 | 2016-06-08 | Population Genetics Technologies Ltd. | Compositions and methods for intramolecular nucleic acid rearrangement |
| US20120276524A1 (en) * | 2009-11-16 | 2012-11-01 | Genomictree, Inc. | Genotyping method |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US9315857B2 (en) | 2009-12-15 | 2016-04-19 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse label-tags |
| EP3115468B1 (en) | 2010-09-21 | 2018-07-25 | Agilent Technologies, Inc. | Increasing confidence of allele calls with molecular counting |
| US9163281B2 (en) * | 2010-12-23 | 2015-10-20 | Good Start Genetics, Inc. | Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction |
| EP2670863B1 (en) | 2011-01-31 | 2018-06-27 | H. Hoffnabb-La Roche Ag | Methods of identifying multiple epitopes in cells |
| US9260753B2 (en) | 2011-03-24 | 2016-02-16 | President And Fellows Of Harvard College | Single cell nucleic acid detection and analysis |
| TWI465572B (zh) * | 2011-05-19 | 2014-12-21 | Univ Chang Gung | Method, composition and system of amplification and detection of target microbial DNA |
| EP2710172B1 (en) | 2011-05-20 | 2017-03-29 | Fluidigm Corporation | Nucleic acid encoding reactions |
| US9228233B2 (en) | 2011-10-17 | 2016-01-05 | Good Start Genetics, Inc. | Analysis methods |
| WO2013130512A2 (en) | 2012-02-27 | 2013-09-06 | The University Of North Carolina At Chapel Hill | Methods and uses for molecular tags |
| GB2513024B (en) | 2012-02-27 | 2016-08-31 | Cellular Res Inc | A clonal amplification method |
| WO2013128281A1 (en) | 2012-02-28 | 2013-09-06 | Population Genetics Technologies Ltd | Method for attaching a counter sequence to a nucleic acid sample |
| US8209130B1 (en) | 2012-04-04 | 2012-06-26 | Good Start Genetics, Inc. | Sequence assembly |
| US10227635B2 (en) | 2012-04-16 | 2019-03-12 | Molecular Loop Biosolutions, Llc | Capture reactions |
| CA2874343C (en) | 2012-05-21 | 2021-11-09 | Fluidigm Corporation | Single-particle analysis of particle populations |
| EP2694669B1 (en) * | 2012-06-28 | 2017-05-17 | Taxon Biosciences, Inc. | Methods for making or creating a synthetic microbial consortium identified by computational analysis of amplicon sequences |
| JP6525872B2 (ja) | 2012-08-08 | 2019-06-05 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 細胞中の複数のエピトープを同定するためのダイナミックレンジを高めること |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
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