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DK2443251T3 - Forbedring af DNA/RNA-ekstraktionsudbytte og ¿ekstraktionskvalitet fra jord med lav biomasse - Google Patents

Forbedring af DNA/RNA-ekstraktionsudbytte og ¿ekstraktionskvalitet fra jord med lav biomasse

Info

Publication number
DK2443251T3
DK2443251T3 DK10723147.4T DK10723147T DK2443251T3 DK 2443251 T3 DK2443251 T3 DK 2443251T3 DK 10723147 T DK10723147 T DK 10723147T DK 2443251 T3 DK2443251 T3 DK 2443251T3
Authority
DK
Denmark
Prior art keywords
dna
improvement
extraction
quality
low biomass
Prior art date
Application number
DK10723147.4T
Other languages
English (en)
Inventor
Jacob Baelum
Carsten Suhr Jacobsen
Original Assignee
Nationale Geol Undersøgelser For Danmark Og Grønland De
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nationale Geol Undersøgelser For Danmark Og Grønland De filed Critical Nationale Geol Undersøgelser For Danmark Og Grønland De
Application granted granted Critical
Publication of DK2443251T3 publication Critical patent/DK2443251T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00Reactions characterised by treatment of reaction samples
    • C12Q2523/30Characterised by physical treatment

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK10723147.4T 2009-06-15 2010-06-15 Forbedring af DNA/RNA-ekstraktionsudbytte og ¿ekstraktionskvalitet fra jord med lav biomasse DK2443251T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP09162686 2009-06-15
US22264909P 2009-07-02 2009-07-02
EP10156628 2010-03-16
PCT/EP2010/058336 WO2010146026A1 (en) 2009-06-15 2010-06-15 Improvement of low-biomass soil dna/rna extraction yield and quality

Publications (1)

Publication Number Publication Date
DK2443251T3 true DK2443251T3 (da) 2014-11-03

Family

ID=43355905

Family Applications (1)

Application Number Title Priority Date Filing Date
DK10723147.4T DK2443251T3 (da) 2009-06-15 2010-06-15 Forbedring af DNA/RNA-ekstraktionsudbytte og ¿ekstraktionskvalitet fra jord med lav biomasse

Country Status (4)

Country Link
US (1) US20120094353A1 (da)
EP (1) EP2443251B1 (da)
DK (1) DK2443251T3 (da)
WO (1) WO2010146026A1 (da)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014150910A1 (en) * 2013-03-15 2014-09-25 Ibis Biosciences, Inc. Dna sequences to assess contamination in dna sequencing
US10577643B2 (en) * 2015-10-07 2020-03-03 Illumina, Inc. Off-target capture reduction in sequencing techniques
EP3199629A1 (en) * 2016-01-30 2017-08-02 Safeguard Biosystems Holdings Ltd. Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms
US10036054B2 (en) 2016-01-30 2018-07-31 Safeguard Biosystems Holdings Ltd. Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms
CN106119104A (zh) * 2016-07-05 2016-11-16 上海市奉贤区中心医院 核酸杂交分析用高效降温装置及方法
JPWO2018012011A1 (ja) * 2016-07-11 2018-07-19 三菱ケミカル株式会社 口腔内検査方法
SG10201913371SA (en) 2017-01-30 2020-03-30 Safeguard Biosystems Holdings Ltd Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3491938B2 (ja) * 1993-12-13 2004-02-03 キヤノン株式会社 Dnaの回収方法

Also Published As

Publication number Publication date
US20120094353A1 (en) 2012-04-19
EP2443251B1 (en) 2014-08-13
EP2443251A1 (en) 2012-04-25
WO2010146026A1 (en) 2010-12-23

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