DK2443251T3 - Forbedring af DNA/RNA-ekstraktionsudbytte og ¿ekstraktionskvalitet fra jord med lav biomasse - Google Patents
Forbedring af DNA/RNA-ekstraktionsudbytte og ¿ekstraktionskvalitet fra jord med lav biomasseInfo
- Publication number
- DK2443251T3 DK2443251T3 DK10723147.4T DK10723147T DK2443251T3 DK 2443251 T3 DK2443251 T3 DK 2443251T3 DK 10723147 T DK10723147 T DK 10723147T DK 2443251 T3 DK2443251 T3 DK 2443251T3
- Authority
- DK
- Denmark
- Prior art keywords
- dna
- improvement
- extraction
- quality
- low biomass
- Prior art date
Links
- 239000002028 Biomass Substances 0.000 title 1
- 238000007400 DNA extraction Methods 0.000 title 1
- 238000002123 RNA extraction Methods 0.000 title 1
- 238000000605 extraction Methods 0.000 title 1
- 239000002689 soil Substances 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2523/00—Reactions characterised by treatment of reaction samples
- C12Q2523/30—Characterised by physical treatment
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP09162686 | 2009-06-15 | ||
| US22264909P | 2009-07-02 | 2009-07-02 | |
| EP10156628 | 2010-03-16 | ||
| PCT/EP2010/058336 WO2010146026A1 (en) | 2009-06-15 | 2010-06-15 | Improvement of low-biomass soil dna/rna extraction yield and quality |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2443251T3 true DK2443251T3 (da) | 2014-11-03 |
Family
ID=43355905
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK10723147.4T DK2443251T3 (da) | 2009-06-15 | 2010-06-15 | Forbedring af DNA/RNA-ekstraktionsudbytte og ¿ekstraktionskvalitet fra jord med lav biomasse |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20120094353A1 (da) |
| EP (1) | EP2443251B1 (da) |
| DK (1) | DK2443251T3 (da) |
| WO (1) | WO2010146026A1 (da) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014150910A1 (en) * | 2013-03-15 | 2014-09-25 | Ibis Biosciences, Inc. | Dna sequences to assess contamination in dna sequencing |
| US10577643B2 (en) * | 2015-10-07 | 2020-03-03 | Illumina, Inc. | Off-target capture reduction in sequencing techniques |
| EP3199629A1 (en) * | 2016-01-30 | 2017-08-02 | Safeguard Biosystems Holdings Ltd. | Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms |
| US10036054B2 (en) | 2016-01-30 | 2018-07-31 | Safeguard Biosystems Holdings Ltd. | Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms |
| CN106119104A (zh) * | 2016-07-05 | 2016-11-16 | 上海市奉贤区中心医院 | 核酸杂交分析用高效降温装置及方法 |
| JPWO2018012011A1 (ja) * | 2016-07-11 | 2018-07-19 | 三菱ケミカル株式会社 | 口腔内検査方法 |
| SG10201913371SA (en) | 2017-01-30 | 2020-03-30 | Safeguard Biosystems Holdings Ltd | Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3491938B2 (ja) * | 1993-12-13 | 2004-02-03 | キヤノン株式会社 | Dnaの回収方法 |
-
2010
- 2010-06-15 DK DK10723147.4T patent/DK2443251T3/da active
- 2010-06-15 US US13/378,303 patent/US20120094353A1/en not_active Abandoned
- 2010-06-15 EP EP10723147.4A patent/EP2443251B1/en active Active
- 2010-06-15 WO PCT/EP2010/058336 patent/WO2010146026A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| US20120094353A1 (en) | 2012-04-19 |
| EP2443251B1 (en) | 2014-08-13 |
| EP2443251A1 (en) | 2012-04-25 |
| WO2010146026A1 (en) | 2010-12-23 |
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