DK2279253T3 - Konstruktion og tilførsel af terapeutiske sammensætninger af frisk isolerede celler - Google Patents
Konstruktion og tilførsel af terapeutiske sammensætninger af frisk isolerede celler Download PDFInfo
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Claims (4)
1. Ex-vivo-fremgangsmåde til transient at modificere ustimulerede hvilende perifere blodmononukleare celler (PBMC'er) til at eksprimere en kimerisk receptor på deres overflade, hvilken fremgangsmåde omfatter følgende trin: (a) isolering af ustimulerede hvilende PBMC'erne fra perifert blod, som tidligere er opsamlet; og (b) elektroladning af PBMC'erne med mRNA, som koder for en kimerisk receptor, hvor de elektroladede PBMC'er transient eksprimerer den kimeriske receptor på deres overflade, og hvor elektroladningen er strømnings-elektroporation.
2. Fremgangsmåde ifølge krav 1, hvor PBMC'erne er perifere blodlymfocyter (PBL'er), naturlige killer-(NK)-celler, eller T-celler.
3. Fremgangsmåde ifølge krav 2, hvor trinnet med isolering af NK-cellerne omfatter (i) separation af CD56+-celler fra blod eller trinnet med (ii) depletering af CD3+-celler fra blod.
4. Fremgangsmåde ifølge krav 1, hvor den kimeriske receptor er en anti-CD19 kimerisk receptor.
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| US4365308P | 2008-04-09 | 2008-04-09 | |
| PCT/US2009/040040 WO2009126789A2 (en) | 2008-04-09 | 2009-04-09 | Engineering and delivery of therapeutic compositions of freshly isolated cells |
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| DK2279253T3 true DK2279253T3 (da) | 2017-02-13 |
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| WO2008097926A2 (en) * | 2007-02-02 | 2008-08-14 | Yale University | Transient transfection with rna |
| US10155038B2 (en) | 2007-02-02 | 2018-12-18 | Yale University | Cells prepared by transient transfection and methods of use thereof |
| US9249423B2 (en) | 2007-02-02 | 2016-02-02 | Yale University | Method of de-differentiating and re-differentiating somatic cells using RNA |
| DK2279253T3 (da) * | 2008-04-09 | 2017-02-13 | Maxcyte Inc | Konstruktion og tilførsel af terapeutiske sammensætninger af frisk isolerede celler |
| PH12013501201A1 (en) * | 2010-12-09 | 2013-07-29 | Univ Pennsylvania | Use of chimeric antigen receptor-modified t cells to treat cancer |
| JP5989016B2 (ja) * | 2011-06-24 | 2016-09-07 | 国立大学法人九州大学 | Ｎｋ細胞の増幅方法 |
| JP5572863B2 (ja) * | 2011-06-24 | 2014-08-20 | 国立大学法人九州大学 | Ｎｋ細胞の増幅方法 |
| EA201490636A1 (ru) | 2011-09-16 | 2014-08-29 | Дзе Трастиз Оф Дзе Юниверсити Оф Пенсильвания | Сконструированные с помощью рнк t-клетки для лечения злокачественных новообразований |
| US9272002B2 (en) | 2011-10-28 | 2016-03-01 | The Trustees Of The University Of Pennsylvania | Fully human, anti-mesothelin specific chimeric immune receptor for redirected mesothelin-expressing cell targeting |
| CA2868121C (en) | 2012-03-23 | 2021-06-01 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Anti-mesothelin chimeric antigen receptors |
| ES2949648T3 (es) | 2012-12-20 | 2023-10-02 | Purdue Research Foundation | Células T que expresan un receptor antigénico quimérico como terapia contra el cáncer |
| US10144770B2 (en) | 2013-10-17 | 2018-12-04 | National University Of Singapore | Chimeric receptors and uses thereof in immune therapy |
| KR102463529B1 (ko) | 2014-04-10 | 2022-11-07 | 시애틀 칠드런즈 호스피탈 디/비/에이 시애틀 칠드런즈 리서치 인스티튜트 | 약물 관련 트랜스진 발현 |
| WO2016095934A2 (en) * | 2014-12-14 | 2016-06-23 | El Abd Hisham Mohamed Magdy | A novel genetic device to engineer cell behavior |
| WO2016123578A1 (en) | 2015-01-30 | 2016-08-04 | The Regents Of The University Of California | Protein delivery in primary hematopoietic cells |
| WO2017004252A1 (en) | 2015-06-30 | 2017-01-05 | The Arizona Board Of Regents On Behalf Of The University Of Arizona | Redirected cells with mhc chimeric receptors and methods of use in immunotherapy |
| KR20240132100A (ko) | 2015-07-28 | 2024-09-02 | 더 트러스티스 오브 더 유니버시티 오브 펜실베니아 | 키메릭 항원 수용체를 발현하는 변형된 단핵세포/대식세포 및 그의 용도 |
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