DK2257623T3 - plasmid - Google Patents
plasmidInfo
- Publication number
- DK2257623T3 DK2257623T3 DK09724492.5T DK09724492T DK2257623T3 DK 2257623 T3 DK2257623 T3 DK 2257623T3 DK 09724492 T DK09724492 T DK 09724492T DK 2257623 T3 DK2257623 T3 DK 2257623T3
- Authority
- DK
- Denmark
- Prior art keywords
- plasmid vector
- plasmid
- asn
- glu
- thr
- Prior art date
Links
- 239000013612 plasmid Substances 0.000 title 1
- 239000013600 plasmid vector Substances 0.000 abstract 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 abstract 1
- 101710188003 Replication and maintenance protein Proteins 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 125000000539 amino acid group Chemical group 0.000 abstract 1
- 238000011031 large-scale manufacturing process Methods 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
Abstract
To provide a plasmid vector having high productivity which can be used for large scale production of an industrially valuable and useful protein, and a transformant using the plasmid vector. A plasmid vector having DNA which encodes a plasmid replication protein in which one or more amino acid residues that are selected from (a) position 48, (b) position 262, (c) position 149 and (d) position 198 in the amino acid sequence represented by SEQ ID NO: 2 are substituted with (a) Ala, Gly, Thr, Arg, Glu, Asn or Gln, (b) Gly, Ser, Thr, Cys or Val, (c) Asn, and (d) Glu, respectively.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2008078080 | 2008-03-25 | ||
| PCT/JP2009/056430 WO2009119876A1 (en) | 2008-03-25 | 2009-03-24 | Plasmid vector |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2257623T3 true DK2257623T3 (en) | 2012-02-06 |
Family
ID=40852157
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK09724492.5T DK2257623T3 (en) | 2008-03-25 | 2009-03-24 | plasmid |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US8389685B2 (en) |
| EP (1) | EP2257623B1 (en) |
| JP (1) | JP5361484B2 (en) |
| CN (1) | CN101978056B (en) |
| AT (1) | ATE540113T1 (en) |
| DK (1) | DK2257623T3 (en) |
| WO (1) | WO2009119876A1 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101762101B1 (en) * | 2009-07-31 | 2017-08-04 | 가부시키가이샤 한도오따이 에네루기 켄큐쇼 | Semiconductor device and method for manufacturing the same |
| JP5735775B2 (en) * | 2009-10-21 | 2015-06-17 | 花王株式会社 | Modified promoter |
| JP5438469B2 (en) | 2009-11-05 | 2014-03-12 | ルネサスエレクトロニクス株式会社 | Load drive device |
| JP7218090B2 (en) * | 2018-01-12 | 2023-02-06 | 花王株式会社 | Protein production method |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01273591A (en) | 1987-08-19 | 1989-11-01 | Agency Of Ind Science & Technol | Human growth hormonal secretory plasmid, transformant using the same plasmid and method for secreting protein |
| JPH06327480A (en) | 1993-05-20 | 1994-11-29 | Mitsubishi Petrochem Co Ltd | DNA fragment containing a gene involved in the function of autonomous replication of plasmid |
| JP3492935B2 (en) | 1999-04-02 | 2004-02-03 | 花王株式会社 | Plasmid vector |
| US6703492B1 (en) * | 1999-11-09 | 2004-03-09 | Smithkline Beecham Corporation | Staphylococcus epidermidis nucleic acids and proteins |
| JP4733298B2 (en) | 2001-07-05 | 2011-07-27 | 三菱レイヨン株式会社 | DNA fragment containing a gene having a function relating to autonomous growth of a plasmid |
| JP4417608B2 (en) * | 2002-04-25 | 2010-02-17 | 花王株式会社 | Mutant alkaline cellulase |
| DE602004031354D1 (en) * | 2003-11-07 | 2011-03-24 | Kao Corp | RECOMBINANT MICROORGANISM |
| JP2006345860A (en) * | 2005-05-20 | 2006-12-28 | Shinshu Univ | Recombinant Bacillus bacteria |
-
2009
- 2009-03-24 WO PCT/JP2009/056430 patent/WO2009119876A1/en not_active Ceased
- 2009-03-24 EP EP09724492A patent/EP2257623B1/en not_active Not-in-force
- 2009-03-24 AT AT09724492T patent/ATE540113T1/en active
- 2009-03-24 US US12/934,395 patent/US8389685B2/en active Active
- 2009-03-24 JP JP2009071513A patent/JP5361484B2/en active Active
- 2009-03-24 DK DK09724492.5T patent/DK2257623T3/en active
- 2009-03-24 CN CN200980109834.3A patent/CN101978056B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2257623A1 (en) | 2010-12-08 |
| CN101978056A (en) | 2011-02-16 |
| US8389685B2 (en) | 2013-03-05 |
| JP5361484B2 (en) | 2013-12-04 |
| JP2009254361A (en) | 2009-11-05 |
| CN101978056B (en) | 2013-05-22 |
| ATE540113T1 (en) | 2012-01-15 |
| WO2009119876A1 (en) | 2009-10-01 |
| EP2257623B1 (en) | 2012-01-04 |
| US20110021751A1 (en) | 2011-01-27 |
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