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DK2167667T3 - Brassica-plante, der omfatter mutante fedtacyl-acp-thioesterase-alleler - Google Patents

Brassica-plante, der omfatter mutante fedtacyl-acp-thioesterase-alleler Download PDF

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Publication number
DK2167667T3
DK2167667T3 DK08773917.3T DK08773917T DK2167667T3 DK 2167667 T3 DK2167667 T3 DK 2167667T3 DK 08773917 T DK08773917 T DK 08773917T DK 2167667 T3 DK2167667 T3 DK 2167667T3
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fatb
seq
ser
mutant
val
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DK08773917.3T
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Benjamin Laga
Boer Bart Den
Bart Lambert
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Bayer Cropscience Nv
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    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • A01H1/045—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection using molecular markers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/20—Brassicaceae, e.g. canola, broccoli or rucola
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/172—Haplotypes

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Claims (6)

1. Brassica-plante eller en celle, en del, et frø eller et afkom deraf, der er kendetegnet ved, at den omfatter mindst tre mutante FATB-alleler i dens genom, som danner frøolie, hvor frøolien har mindre end 7 vægt-% mættede fedtsyrer baseret på den totale mængde af fedtsyrer i frøolien, hvor de mutante FATB-alleler er mutante alleler af et gen, der koder for et funktionelt FATB-protein, hvor det funktionelle FATB-qen omfatter et nukleinsyremolekyle, der er udvalgt fra gruppen, der består af: et nukleinsyremolekyle der omfatter mindst 90 % sekvensidentitet med SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9 eller SEQ ID NO: 11; og - et nukleinsyremolekyle, der koder for en aminosyresekvens, der omfatter mindst 90 % sekvensidentitet med SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10 eller SEQ ID NO: 12, hvor de mutante FATB-alleler omfatter et muteret DNA-område, der består af et eller flere indsatte, deleterede eller substituerede nukleotider sammenlignet med et tilsvarende vildtype-DNA-område i det funktionelle FATB-qen, og hvor de mutante FATB-alleler koder for et ikke-funktionelt protein, der ikke har nogen acyl-ACP-thioesteraseaktivitet in vivo, eller ikke koder for et protein.
2. Brassica-plante eller en celle, en del, et frø eller et afkom deraf ifølge krav 1, hvor de mutante FATB-alleler er udvalgt fra gruppen, der består af: - en mutant FATB-allel, der omfatter en deletion af et FATB-qen, der har sekvensen ifølge SEQ ID NO: 13; - en mutant FATB-allel, der omfatter en deletion af et FATB-qen, der har sekvensen ifølge SEQ ID NO: 15; - en mutant FATB-allel, der omfatter en deletion af et FATB-qen, der har sekvensen ifølge SEQ ID NO: 21; et nukleinsyremolekyle ifølge SEQ ID NO: 13, hvor g i position 279 er substitueret med a; et nukleinsyremolekyle ifølge SEQ ID NO: 13, hvor g i position 333 er substitueret med a; et nukleinsyremolekyle ifølge SEQ ID NO: 13, hvor g i position 348 er substitueret med a; et nukleinsyremolekyle ifølge SEQ ID NO: 15, hvor g i position 282 er substitueret med a; et nukleinsyremolekyle ifølge SEQ ID NO: 15, hvor c i position 406 er substitueret med t; et nukleinsyremolekyle ifølge SEQ ID NO: 17, hvor c i position 845 er substitueret med t; et nukleinsyremolekyle ifølge SEQ ID NO: 19, hvor g i position 668 er substitueret med a; et nukleinsyremolekyle ifølge SEQ ID NO: 21, hvor c i position 235 er substitueret med t; et nukleinsyremolekyle ifølge SEQ ID NO: 21, hvor c i position 331 er substitueret med t; et nukleinsyremolekyle ifølge SEQ ID NO: 21, hvor g i position 336 er substitueret med a; et nukleinsyremolekyle ifølge SEQ ID NO: 13, hvor g i position 502 er substitueret med a; et nukleinsyremolekyle ifølge SEQ ID NO: 13, hvor g i position 587 er substitueret med a; et nukleinsyremolekyle ifølge SEQ ID NO: 15, hvor g i position 505 er substitueret med a; et nukleinsyremolekyle ifølge SEQ ID NO: 15, hvor g i position 504 er substitueret med a; et nukleinsyremolekyle ifølge SEQ ID NO: 19, hvor g i position 498 er substitueret med a; et nukleinsyremolekyle ifølge SEQ ID NO: 21, hvor g i position 581 er substitueret med a; og et nukleinsyremolekyle ifølge SEQ ID NO: 23, hvor g i position 508 er substitueret med a.
3. Plante ifølge krav 1 eller 2, hvor frøolien har lig med eller mindre end 3,5 vægt-% mættede fedtsyrer, især palmitinsyre og/eller stearinsyre, baseret på den totale mængde af fedtsyrer i frøolien.
4. Plante ifølge et hvilket som helst af kravene 1 til 3, hvor planten er en Brassica-oliefrøart, fortrinsvis Brassica napus, Brassica juncea eller Brassica rapa.
5. Fremgangsmåde til produktion af frøolie, der omfatter trinene høst af frø fra en Brassica-plante, der omfatter mindst tre mutante FALB-alleler ifølge krav 1 eller 2 i dens genom, og ekstraktion af olien fra frøene.
6. Brassica-plante eller en celle, en del, et frø eller et afkom deraf, - der omfatter en nukleinsyresekvens ifølge SEQ ID NO: 13, hvor g i position 333 er substitueret med a, en nukleinsyresekvens ifølge SEQ ID NO: 17, hvor c i position 845 er substitueret med t, en nukleinsyresekvens ifølge SEQ ID NO: 19, hvor g i position 498 er substitueret med a, og en nukleinsyresekvens ifølge SEQ ID NO: 23, hvor g i position 508 er substitueret med a, hvoraf reference-Brassica-frø er blevet deponeret ved NCIMB Limited den 27. juni 2008 under accessionsnummer NCIMB 41568, - der omfatter en nukleinsyresekvens ifølge SEQ ID NO: 13, hvor g i position 348 er substitueret med a, en nukleinsyresekvens ifølge SEQ ID NO: 15, hvor c i position 406 er substitueret med t, en nukleinsyresekvens ifølge SEQ ID NO: 19, hvor g i position 668 er substitueret med a, og en nukleinsyresekvens ifølge SEQ ID NO: 21, hvor g i position 336 er substitueret med a, hvoraf reference-Brassica-frø er blevet deponeret ved NCIMB Limited den 27. juni 2008 under accessionsnummer NCIMB 41567, eller - der omfatter en nukleinsyresekvens ifølge SEQ ID NO: 15, hvor g i position 282 er substitueret med a, en nukleinsyresekvens ifølge SEQ ID NO: 19, hvor g i position 668 er substitueret med a, og en nukleinsyresekvens ifølge SEQ ID NO: 21, hvor c i position 235 er substitueret med t, hvoraf reference-Brassica-frø er blevet deponeret ved NCIMB Limited den 27. juni 2008 under accessionsnummer NCIMB 41566.
DK08773917.3T 2007-07-09 2008-07-07 Brassica-plante, der omfatter mutante fedtacyl-acp-thioesterase-alleler DK2167667T3 (da)

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EP07075568 2007-07-09
US95894507P 2007-07-10 2007-07-10
PCT/EP2008/005551 WO2009007091A2 (en) 2007-07-09 2008-07-07 Brassica plant comprising mutant fatty acyl-acp thioesterase alleles

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US (2) US8981180B2 (da)
EP (1) EP2167667B1 (da)
JP (1) JP5677839B2 (da)
CN (1) CN101688220B (da)
AR (1) AR067480A1 (da)
AU (1) AU2008274551B2 (da)
CA (2) CA3128584A1 (da)
DK (1) DK2167667T3 (da)
EA (1) EA030862B1 (da)
ES (1) ES2545083T3 (da)
PL (1) PL2167667T3 (da)
SI (1) SI2167667T1 (da)
UA (1) UA99471C2 (da)
WO (1) WO2009007091A2 (da)
ZA (2) ZA200908886B (da)

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EP2167667B1 (en) 2015-05-20
JP2010532667A (ja) 2010-10-14
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WO2009007091A3 (en) 2009-07-23
UA99471C2 (ru) 2012-08-27

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