DK2062051T3 - Fremgangsmåder og anordninger til detektering af strukturændringer i et molekyle ved måling af elektrokemisk impedans - Google Patents
Fremgangsmåder og anordninger til detektering af strukturændringer i et molekyle ved måling af elektrokemisk impedans Download PDFInfo
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- 238000002331 protein detection Methods 0.000 description 1
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- 210000003296 saliva Anatomy 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
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- 230000011664 signaling Effects 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00653—Making arrays on substantially continuous surfaces the compounds being bound to electrodes embedded in or on the solid supports
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00659—Two-dimensional arrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/0068—Means for controlling the apparatus of the process
- B01J2219/00702—Processes involving means for analysing and characterising the products
- B01J2219/00704—Processes involving means for analysing and characterising the products integrated with the reactor apparatus
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00725—Peptides
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Claims (13)
1. Fremgangsmåde til detektering af en enheds binding til et molekyle, hvilket molekyle er bundet til en overflade, hvilken overflade er elektrisk ledende, hvor fremgangsmåden omfatter monitorering af fasen med elektrokemisk impedans på overfladen som en frekvensfunktion, og detektering af ændring som en frekvensfunktion i den monitorerede fase i den elektrokemiske impedans på overfladen som en frekvensfunktion, der indikerer bindingen af enheden til molekylet, hvilken ændring afhænger af koncentrationen af enheden.
2. Fremgangsmåde ifølge krav 1, hvor fasen er monitoreret som en frekvensfunktion mellem 0,1 Hz og 500 kHz.
3. Fremgangsmåde ifølge krav 1 eller 2, hvor overfladen omfatter en elektrode.
4. Fremgangsmåde ifølge krav 3, hvor elektroden omfatter metal.
5. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor molekylet omfatter et polypeptid.
6. Fremgangsmåde ifølge krav 5, hvor fremgangsmåden endvidere omfatter: (a) tilvejebringelse af en række, der omfatter mindst to elektroder, hvor elektroderne har en eller flere af: (i) en diameter på 100 pm eller mindre, og (ii) en afstand på 100 pm eller mindre; og (b) binding af polypeptidet til mindst én elektrode fra rækken, hvorved overfladen dannes.
7. Fremgangsmåde ifølge krav 6, hvor rækken omfatter mindst 10 individuelt adresserbare elektroder.
8. Fremgangsmåde ifølge krav 6 eller 7, hvor elektroderne har en eller flere af: (i) en diameter på 20 pm eller mindre, og (ii) en afstand på 15 pm eller mindre.
9. Fremgangsmåde ifølge et hvilket som helst af kravene 6 til 8, hvor elektroderne har både (i) og (ii).
10. Fremgangsmåde ifølge et hvilket som helst af kravene 5 til 9, hvor polypeptidet omfatter et scaffoldprotein.
11. Fremgangsmåde ifølge et hvilket som helst af kravene 5 til 10, hvor polypeptidet omfatter en peptidaptamer.
12. Fremgangsmåde ifølge et hvilket som helst af kravene 5 til 11, hvor polypeptidet er bundet til elektroden ved hjælp af en thiolbinding.
13. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 4, hvor molekylet omfatter DNA eller en anden nukleinsyre.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0618253A GB0618253D0 (en) | 2006-09-16 | 2006-09-16 | Methods and devices for sensing molecular interactions |
| GB0620808A GB0620808D0 (en) | 2006-10-20 | 2006-10-20 | Methods and devices for sensing molecular interactions |
| PCT/GB2007/003465 WO2008032066A1 (en) | 2006-09-16 | 2007-09-13 | Methods and devices for detecting structural changes in a molecule measuring electrochemical impedance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2062051T3 true DK2062051T3 (da) | 2016-05-09 |
Family
ID=38820275
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK07804258.7T DK2062051T3 (da) | 2006-09-16 | 2007-09-13 | Fremgangsmåder og anordninger til detektering af strukturændringer i et molekyle ved måling af elektrokemisk impedans |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US20100234234A1 (da) |
| EP (1) | EP2062051B1 (da) |
| DK (1) | DK2062051T3 (da) |
| ES (1) | ES2562666T3 (da) |
| WO (1) | WO2008032066A1 (da) |
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| CA2851913A1 (en) | 2011-10-18 | 2013-04-25 | Twistnostics Llc | Detection units and methods for detecting a target analyte |
| GB201207583D0 (en) | 2012-05-01 | 2012-06-13 | Isis Innovation | Electrochemical detection method and related aspects |
| EP3058372B1 (en) | 2014-02-06 | 2023-07-05 | Scanogen Inc. | Detection units and methods for detecting a target analyte |
| WO2016061590A1 (en) | 2014-10-17 | 2016-04-21 | Wardell Mark R | Method for detecting proteases and active infection in biological fluids and tissues |
| WO2019226841A1 (en) | 2018-05-25 | 2019-11-28 | Scanogen Inc. | Detection units and methods for detecting a target analyte |
| WO2023186363A1 (en) * | 2022-03-30 | 2023-10-05 | Bayer Aktiengesellschaft | Biosensors for the identification of nematode species |
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| US5958791A (en) * | 1996-09-27 | 1999-09-28 | Innovative Biotechnologies, Inc. | Interdigitated electrode arrays for liposome-enhanced immunoassay and test device |
| US20020090649A1 (en) * | 1999-12-15 | 2002-07-11 | Tony Chan | High density column and row addressable electrode arrays |
| US6824669B1 (en) * | 2000-02-17 | 2004-11-30 | Motorola, Inc. | Protein and peptide sensors using electrical detection methods |
| AU2001252973A1 (en) * | 2000-04-17 | 2001-10-30 | Purdue Research Foundation | Biosensor and related method |
| GB0223666D0 (en) * | 2002-10-10 | 2002-11-20 | Univ Cambridge Tech | Arrays of molecules and their production |
| US20050059105A1 (en) * | 2003-07-25 | 2005-03-17 | Board Of Trustees Of Michigan State University | Impedimetric biosensor and its use for rapid detection of bacterial pathogens in solution |
| US7718579B2 (en) * | 2004-09-13 | 2010-05-18 | Combimatrix Corporation | Electrochemical deblocking using a hydrazine derivative |
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2007
- 2007-09-13 EP EP07804258.7A patent/EP2062051B1/en not_active Not-in-force
- 2007-09-13 WO PCT/GB2007/003465 patent/WO2008032066A1/en not_active Ceased
- 2007-09-13 ES ES07804258.7T patent/ES2562666T3/es active Active
- 2007-09-13 US US12/440,832 patent/US20100234234A1/en not_active Abandoned
- 2007-09-13 DK DK07804258.7T patent/DK2062051T3/da active
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2012
- 2012-09-28 US US13/630,391 patent/US9494583B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| ES2562666T3 (es) | 2016-03-07 |
| US20130040848A1 (en) | 2013-02-14 |
| EP2062051A1 (en) | 2009-05-27 |
| EP2062051B1 (en) | 2016-02-10 |
| US9494583B2 (en) | 2016-11-15 |
| WO2008032066A1 (en) | 2008-03-20 |
| US20100234234A1 (en) | 2010-09-16 |
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