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DK1781772T3 - Metabolisk modificering af xylose-fermenterende eukaryote celler - Google Patents

Metabolisk modificering af xylose-fermenterende eukaryote celler Download PDF

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Publication number
DK1781772T3
DK1781772T3 DK05763114.5T DK05763114T DK1781772T3 DK 1781772 T3 DK1781772 T3 DK 1781772T3 DK 05763114 T DK05763114 T DK 05763114T DK 1781772 T3 DK1781772 T3 DK 1781772T3
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DK05763114.5T
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Aaron Adriaan Winkler
Sipko Maarten Kuyper
Laat Wilhelmus Theodorus Antonius Maria De
Dijken Johannes Pieter Van
Jacobus Thomas Pronk
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Dsm Ip Assets Bv
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (21)

1. Gær eller trådformet svampeværtscelle, der er transformeret med en nukleinsyrekonstruktion, der omfatter en nukleotidsekvens, der koder for en xyloseisomerase, hvilken xyloseisomerase omfatter en aminosyresekvens, der har mindst 95 % sekvensidentitet med aminosyresekvensen ifølge SEQ ID NO: 2, hvor nukleinsyrekonstruktionen ved transformation af værtscellen bibringer værtscellen evnen til direkte isomerisering af xylose til xylulose, hvor værtscellen omfatter en genmodifikation, der øger strømningen via pentosephosphat-reaktionsvejen, og hvor genmodifikationen omfatter overekspression af mindst ét gen i den ikke-oxidative del af pentosephosphat-reaktionsvejen sammenlignet med en værtscelle, der er genetisk identisk bortset fra genmodifikationen.
2. Værtscelle ifølge krav 1, hvor nukleotidsekvensen koder for en xyloseisomerase, der kan opnås fra en bakterie fra klassen Bacteroides.
3. Værtscelle ifølge krav 1 eller 2, hvor genet i den ikke-oxidative del af pentosephosphat-reaktionsvejen er udvalgt fra gruppen, der består af generne, der koder for ribulose-5-phosphatisomerase, ribulose-5-phosphatepimerase, transketolase og transaldolase.
4. Værtscelle ifølge krav 3, hvor genmodifikationen omfatter overekspression af mindst generne, der koder for en transketolase og en transaldolase.
5. Værtscelle ifølge et hvilket som helst af kravene 1-4, hvor værtscellen yderligere omfatter en genmodifikation, der øger den specifikke xylulosekinaseaktivitet.
6. Værtscelle ifølge krav 5, hvor genmodifikationen omfatter overekspression af et gen, der koder for en xylulosekinase.
7. Værtscelle ifølge et hvilket som helst af kravene 1-6, hvor genet, der overudtrykkes, er endogent for værtscellen.
8. Værtscelle ifølge et hvilket som helst af kravene 1-7, hvor værtscellen omfatter en genmodifikation, der reducerer uspecifik aldosereductaseaktivitet i værtscellen.
9. Værtscelle ifølge krav 8, hvor genmodifikationen reducerer ekspressionen af eller inaktiverer et gen, der koder for en uspecifik aldosereductase.
10. Værtscelle ifølge krav 9, hvor genet er inaktiveret ved deletion af mindst en del af genet eller ved destruktion af genet.
11. Værtscelle ifølge krav 8 eller 9, hvor ekspressionen af hvert gen i værtscellen, der koder for en uspecifik aldosereductase, er reduceret eller inaktiveret.
12. Værtscelle ifølge et hvilket som helst af ovennævnte krav, hvor værtscellen er en gær, fortrinsvis en gær, der tilhører en af slægterne: Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces og Yarrowia.
13. Værtscelle ifølge krav 12, hvor gæren tilhører en af arterne: S. cerevisiae, S. bulderi, S. barnetti, S. exiguus, S. uvarum, S. diastaticus, K. lactis, K. marxianus og K. fragilis.
14. Værtscelle ifølge et hvilket som helst af kravene 1-11, hvor værtscellen er en trådformet svamp, fortrinsvis en trådformet svamp, der tilhører en af slægterne: Aspergillus, Trichoderma, Humicola, Acremonium, Fusarium og Penicillium.
15. Værtscelle ifølge et hvilket som helst af ovennævnte krav, hvor værtscellen udtrykker et eller flere enzymer, der bibringer værtscellen evnen til at danne mindst ét fermenteringsprodukt udvalgt fra gruppen, der består af ethanol, mælkesyre, 3-hydroxypropionsyre, acrylsyre, eddikesyre, ravsyre, citronsyre, aminosyrer, 1,3-propandiol, ethylen, glycerol, β-lactam-antibiotika og cephalosporiner.
16. Fremgangsmåde til fremstillng af ethanol, hvilken fremgangsmåde omfatter trinene: (a) fermentering af et medium, der indeholder en kilde af xylose, med en modificeret værtscelle ifølge et hvilket som helst af kravene 1-14, hvor værtscellen fermenterer xylose til ethanol, og eventuelt (b) indvinding af ethanol.
17. Fremgangsmåde ifølge krav 16, hvor mediet også indeholder en kilde af glucose.
18. Fremgangsmåde ifølge krav 16 eller 17, hvor den volumetriske produktivitet af ethanol er mindst 0,5 g ethanol pr. liter pr. time.
19. Fremgangsmåde ifølge et hvilket som helst af kravene 16-18, hvor ethanoludbyttet er mindst 50 %.
20. Fremgangsmåde til fremstilling af et fermenteringsprodukt udvalgt fra gruppen, der består af mælkesyre, 3- hydroxypropionsyre, acrylsyre, eddikesyre, ravsyre, citronsyre, aminosyrer, 1,3-propandiol, ethylen, glycerol, β-lactam-antibiotika og cephalosporiner, hvilken fremgangsmåde omfatter trinene: (a) fermentering at et medium, der indeholder en kilde af xylose, med en modificeret værtscelle ifølge krav 15, hvor værtscellen fermenterer xylose til fermenteringsproduktet, og eventuelt (b) indvinding af fermenteringproduktet.
21. Fremgangsmåde ifølge krav 20, hvor mediet også indeholder en kilde af glucose.
DK05763114.5T 2004-07-16 2005-07-15 Metabolisk modificering af xylose-fermenterende eukaryote celler DK1781772T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US58838104P 2004-07-16 2004-07-16
EP04077073 2004-07-16
US58983304P 2004-07-22 2004-07-22
PCT/NL2005/000516 WO2006009434A1 (en) 2004-07-16 2005-07-15 Metabolic engineering of xylose fermenting eukaryotic cells

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US (3) US8034591B2 (da)
EP (1) EP1781772B1 (da)
JP (2) JP5796925B2 (da)
CN (2) CN101052706B (da)
BR (1) BRPI0513437A (da)
CA (2) CA2573483C (da)
DK (1) DK1781772T3 (da)
ES (1) ES2607891T3 (da)
PL (1) PL1781772T3 (da)
WO (1) WO2006009434A1 (da)

Families Citing this family (123)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR0306740A (pt) 2002-01-23 2004-12-28 Royal Nedalco B V Célula hospedeira transformada com um construto de ácido nucléico, molécula de ácido nucleico isolada, e, processos para a produção de etanol, e de um produto de fermentação
US8034591B2 (en) * 2004-07-16 2011-10-11 Techische Universiteit Delft Xylose-fermenting yeast engineered to increase flux of the pentose phosphate pathway
EP1873248A1 (en) * 2006-06-26 2008-01-02 DSMIP Assets B.V. Biological process using a glucose-6-phosphate isomerase
EP1873246A1 (en) * 2006-06-26 2008-01-02 DSMIP Assets B.V. Biological process using a transaldolase
PL2069476T3 (pl) * 2006-10-02 2017-02-28 Dsm Ip Assets B.V. Metaboliczne opracowywanie komórek drożdży fermentujących arabinozę
US20100086965A1 (en) 2006-10-02 2010-04-08 Van Maris Antonius Jeroen Adriaan Metabolic engineering of arabinose-fermenting yeast cells
WO2008144012A2 (en) * 2007-05-14 2008-11-27 The Regents Of The University Of California Xylose- and arabinose- metabolizing enzyme -encoding nucleotide sequences with refined translational kinetics and methods of making same
WO2009011591A2 (en) * 2007-07-19 2009-01-22 Royal Nedalco B.V. Novel arabinose-fermenting eukaryotic cells
EP2062967A1 (en) 2007-11-21 2009-05-27 Technical University of Denmark Genetically engineered aspergillus
JP5625199B2 (ja) * 2008-03-07 2014-11-19 ディーエスエム アイピー アセッツ ビー．ブイ． ペントース糖発酵細胞
WO2009109634A1 (en) * 2008-03-07 2009-09-11 Dsm Ip Assets B.V. A pentose sugar fermenting cell
WO2009109631A1 (en) * 2008-03-07 2009-09-11 Dsm Ip Assets B.V. A pentose sugar fermenting cell
WO2009109630A1 (en) * 2008-03-07 2009-09-11 Dsm Ip Assets B.V. A pentose sugar fermenting cell
GB0812318D0 (en) 2008-07-04 2008-08-13 Terranol As Microorganism
WO2010014817A2 (en) * 2008-07-30 2010-02-04 Novozymes A/S Producing fermentation products
CN108315272A (zh) * 2008-11-21 2018-07-24 拉勒曼德匈牙利流动性管理有限责任公司 用于使用纤维素进行同时糖化和发酵的表达纤维素酶的酵母
GB0822937D0 (en) 2008-12-16 2009-01-21 Terranol As Microorganism
CN102307987B (zh) * 2008-12-24 2016-11-16 帝斯曼知识产权资产管理有限公司 木糖异构酶基因及其在戊糖发酵中的用途
CA2761877A1 (en) * 2009-02-23 2010-08-26 Kirin Holdings Kabushiki Kaisha Method for production of substance in candida utilis using xylose as carbon source
EP2401358B1 (en) 2009-02-25 2019-01-02 Indiana University Research&technology Corporation Materials and methods for identifying and using yeast strains that metabolize pentose sugars in the presence of d-glucose
WO2010147642A1 (en) 2009-06-15 2010-12-23 Massachusetts Institute Of Technology Production of triacylglycerides, fatty acids, and their derivatives
BRPI1011934A8 (pt) * 2009-07-09 2017-10-03 Verdezyne Inc Composição
UA108853C2 (uk) 2009-07-10 2015-06-25 Спосіб ферментації галактози
EP2277989A1 (en) 2009-07-24 2011-01-26 Technische Universiteit Delft Fermentative glycerol-free ethanol production
WO2011065539A1 (ja) * 2009-11-30 2011-06-03 国立大学法人神戸大学 バイオマスからのエタノールの生産方法
US8772012B2 (en) 2009-12-22 2014-07-08 Kabushiki Kaisha Toyota Chuo Kenkyusho Xylose isomerase and use thereof
DK2561064T3 (da) 2010-04-21 2018-01-15 Dsm Ip Assets Bv Celle, som er egnet til fermentering af en sammensætning af blandede sukre
BR112012028290B1 (pt) 2010-05-05 2021-02-02 Lallemand Hungary Liquidity Management Llc. levedura recombinante, processo para converter biomassa em etanol e meio de fermentação compreendendo dita levedura
WO2011149353A1 (en) 2010-05-27 2011-12-01 C5 Yeast Company B.V. Yeast strains engineered to produce ethanol from acetic acid and glycerol
EP2576762A4 (en) 2010-06-03 2013-12-04 Mascoma Corp YEAST FOR THE EXPRESSION OF SACCHAROLYTIC ENZYMES FOR CONSOLIDATED BIOVERIC PROCESSING USING STARCH AND CELLULOSE
US8679822B2 (en) * 2010-06-29 2014-03-25 E I Du Pont De Nemours And Company Xylose utilization in recombinant zymomonas
US10138504B2 (en) 2010-08-06 2018-11-27 Lallemand Hungary Liquidity Management Llc Production of malonyl-CoA derived products via anaerobic pathways
CN102399804A (zh) * 2010-09-15 2012-04-04 中国农业科学院作物科学研究所 木糖异构酶基因的功能及其应用
KR20130100169A (ko) 2010-10-13 2013-09-09 디에스엠 아이피 어셋츠 비.브이. 펜토스 및 글루코스 발효 효모 세포
EP2663645B1 (en) 2010-11-18 2014-12-17 DSM IP Assets B.V. Yeast strains engineered to produce ethanol from glycerol
JP2012120491A (ja) * 2010-12-09 2012-06-28 Toyota Motor Corp キシロースを含む培地における発酵培養方法
CA2822654A1 (en) 2010-12-22 2012-06-28 Mascoma Corporation Genetically modified clostridium thermocellum engineered to ferment xylose
CN102174549B (zh) 2011-02-22 2012-10-10 山东大学 一种编码木糖异构酶的核酸分子及其编码的木糖异构酶
WO2012125027A1 (en) 2011-03-14 2012-09-20 Dsm Ip Assets B.V. Yeast strains that ferment uronic acids
BR122019017739B1 (pt) 2011-04-05 2021-06-22 Lallemand Hungary Liquidity Management Llc Micro-organismo recombinante compreendendo uma deleção de enzimas nativas que atuam para produzir glicerol e/ou regular a síntese de glicerol e vias metabólicas sintéticas para converter uma fonte de carboidrato a etanol
AR086471A1 (es) 2011-04-22 2013-12-18 Dsm Ip Assets Bv Celula de levadura capaz de convertir azucares que incluyen arabinosa y xilosa
EP2546336A1 (en) 2011-07-11 2013-01-16 DSM IP Assets B.V. Yeast strains that consume uronic acids and produce fermentation products such as ethanol
ES2648865T3 (es) 2011-11-30 2018-01-08 Dsm Ip Assets B.V. Cepas de levadura manipuladas para producir etanol a partir de ácido acético y glicerol
EA201400836A8 (ru) 2012-01-23 2016-03-31 ДСМ АйПи АССЕТС Б.В. Получение дитерпена
ES2698500T3 (es) * 2012-03-06 2019-02-05 Lygos Inc Células huésped recombinantes para la producción de malonato
JP6249391B2 (ja) * 2012-06-15 2017-12-20 国立研究開発法人農業・食品産業技術総合研究機構 キシロースを高温で発酵する方法
WO2014033018A1 (en) 2012-08-28 2014-03-06 Dsm Ip Assets B.V. Yeast strains engineered to produce ethanol from acetate
WO2014033019A1 (en) 2012-08-28 2014-03-06 Dsm Ip Assets B.V. Yeast strains engineered to produce ethanol from acetate
US9745560B2 (en) 2012-08-29 2017-08-29 Lallemand Hungary Liquidity Management Llc Expression of enzymes in yeast for lignocellulose derived oligomer CBP
WO2014060377A1 (en) 2012-10-16 2014-04-24 Dsm Ip Assets B.V. Cells with improved pentose conversion
US10604773B2 (en) 2012-10-16 2020-03-31 Dsm Ip Assets B.V. Cells with improved pentose conversion
US20150252319A1 (en) 2012-11-07 2015-09-10 Dsm Ip Assets B.V. pH CONTROLLED YEAST PROPAGATION
BR112015010134A2 (pt) 2012-11-09 2017-08-22 Lallemand Hungary Liquidity Man Llc Processo para consumo de acetato durante a fermentação etanólica de matérias-primas celulósicas
WO2014160402A1 (en) 2013-03-14 2014-10-02 Mascoma Corporation Co-conversion of carbohydrates to fermentation products in a single fermentation step
WO2014142647A1 (en) 2013-03-14 2014-09-18 Wageningen Universiteit Fungals strains with improved citric acid and itaconic acid production
DE102013205986A1 (de) 2013-04-04 2014-10-23 Wacker Chemie Ag Mikroorganismenstamm und Verfahren zur fermentativen Herstellung von C4-Verbindungen aus C5-Zuckern
MX367033B (es) 2013-05-31 2019-08-02 Dsm Ip Assets Bv Microorganismos para la produccion de diterpeno.
CA2914900A1 (en) 2013-07-15 2015-01-22 Dsm Ip Assets B.V. Recombinant microorganism and process for the production of steviol glycosides
DK3027632T3 (da) 2013-07-31 2019-06-17 Dsm Ip Assets Bv Indvinding af steviolglycosider fra et fermenteringsmedium
US11753656B2 (en) 2013-08-15 2023-09-12 Lallemand Hungary Liquidity Management Llc Methods for the improvement of product yield and production in a microorganism through glycerol recycling
AR097480A1 (es) 2013-08-29 2016-03-16 Dsm Ip Assets Bv Células de levadura convertidoras de glicerol y ácido acético con una conversión de ácido acético mejorada
WO2015066642A1 (en) * 2013-11-04 2015-05-07 The Board Of Trustees Of The Leland Stanford Junior University Benzylisoquinoline alkaloid (bia) precursor producing microbes, and methods of making and using the same
US20180030482A1 (en) 2014-04-18 2018-02-01 Moralco B.V. Use of acetaldehyde in the fermentative production of ethanol
BR102014014407A2 (pt) 2014-06-12 2016-04-19 Biocelere Agroindustrial Ltda cassete de expressão para transformar célula eucariótica, micro-organismo geneticamente modificado com eficiente consumo de xilose, processo para produção de biocombustíveis e bioquímicos e biocombustível e/ou bioquímico assim produzido
WO2016012429A1 (en) 2014-07-24 2016-01-28 Dsm Ip Assets B.V. Yeast cell with improved pentose transport
BR102014027233A2 (pt) * 2014-10-30 2016-05-24 Biocelere Agroindustrial Ltda cassete de expressão, micro-organismo geneticamente modificado para expressão de xilose isomerase, processo para produção de biocombustíveis e/ou bioquímicos e biocombustível e/ou bioquímicos produzidos
EP3250698B1 (en) 2015-01-28 2019-11-27 DSM IP Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
CN107429265A (zh) 2015-01-28 2017-12-01 帝斯曼知识产权资产管理有限公司 酶促水解木质纤维素材料和发酵糖的方法
WO2016120298A1 (en) 2015-01-28 2016-08-04 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
US10604743B2 (en) 2015-03-16 2020-03-31 Dsm Ip Assets B.V. UDP-glycosyltransferases
EP3274448B1 (en) 2015-03-23 2021-08-11 DSM IP Assets B.V. Udp-glycosyltransferases from solanum lycopersicum
US11344051B2 (en) 2015-04-03 2022-05-31 Dsm Ip Assets B.V. Steviol glycosides
WO2016169892A1 (en) 2015-04-20 2016-10-27 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2016169893A1 (en) 2015-04-20 2016-10-27 Dsm Ip Assets B.V. Whole fermentation broth
CA2980649A1 (en) 2015-04-21 2016-10-27 Dsm Ip Assets B.V. Geranylgeranyl pyrophosphate synthase
WO2016207144A1 (en) 2015-06-22 2016-12-29 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
EP3320104B1 (en) 2015-07-10 2021-11-10 DSM IP Assets B.V. Method for preparing a steviol glycoside composition
HK1254383A1 (zh) 2015-07-13 2019-07-19 MARA Renewables Corporation 增强木糖的微藻代谢
WO2017024150A1 (en) 2015-08-05 2017-02-09 Cargill, Incorporated Xylose isomerase-modified yeast strains and methods for bioproduct production
CN107922913B (zh) 2015-08-13 2022-04-05 帝斯曼知识产权资产管理有限公司 甜菊醇糖苷转运
CN106554924B (zh) * 2015-09-24 2019-12-06 中粮营养健康研究院有限公司 生产乙醇的重组酿酒酵母菌株、其构建方法以及利用该菌株生产乙醇的方法
EP4043558A3 (en) 2015-10-05 2022-11-16 DSM IP Assets B.V. Kaurenoic acid hydroxylases
MX2018005692A (es) * 2015-11-06 2019-03-14 Lallemand Hungary Liquidity Man Llc Limitación de trehalosa producida por levaduras en fermentación.
CN105838744A (zh) * 2016-04-26 2016-08-10 上海交通大学 制备2-表-5-表有效醇酮的方法
US11142783B2 (en) 2016-06-09 2021-10-12 Dsm Ip Assets B.V. Seed train for large scale enzyme production
US10913938B2 (en) 2016-07-29 2021-02-09 Dsm Ip Assets B.V. Polypeptides having cellulolytic enhancing activity and uses thereof
CA3032656A1 (en) 2016-08-09 2018-02-15 Dsm Ip Assets B.V. Crystallization of steviol glycosides
WO2018029274A1 (en) 2016-08-09 2018-02-15 Dsm Ip Assets B.V. Crystallization of steviol glycosides
CA3040585A1 (en) 2016-10-27 2018-05-03 Dsm Ip Assets B.V. Geranylgeranyl pyrophosphate synthases
US12188070B2 (en) 2016-11-04 2025-01-07 New Energy Blue Llc Method for preparing fermentable sugars from lignocellulosic biomass
CA3043435A1 (en) 2016-11-24 2018-05-31 Dsm Ip Assets B.V. Enzyme compositions with improved hydrolysis performance
MY198644A (en) 2016-11-24 2023-09-13 Dsm Ip Assets Bv Enzyme composition
WO2018104238A1 (en) 2016-12-08 2018-06-14 Dsm Ip Assets B.V. Kaurenoic acid hydroxylases
US11692187B2 (en) 2016-12-21 2023-07-04 Vib Vzw Xylose isomerases that confer efficient xylose fermentation capability to yeast
CN110177879A (zh) * 2016-12-22 2019-08-27 拉勒曼德匈牙利流动性管理有限责任公司 重组酵母宿主细胞的亚硫酸盐耐受性
WO2018114995A1 (en) 2016-12-22 2018-06-28 Dsm Ip Assets B.V. Fermentation process for producing steviol glycosides
WO2018185071A1 (en) 2017-04-03 2018-10-11 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
EP3645714B1 (en) 2017-06-27 2022-08-31 DSM IP Assets B.V. Udp-glycosyltransferases
CA3073387A1 (en) * 2017-08-29 2019-03-07 Danisco Us Inc Modified yeast comprising glucose-specific, atp-mediated transporters
PL3695001T3 (pl) 2017-10-09 2024-05-27 Versalis S.P.A. Sposób hydrolizy enzymatycznej materiału lignocelulozowego i fermentacji cukrów
WO2019086369A1 (en) 2017-10-30 2019-05-09 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2019086370A1 (en) 2017-10-30 2019-05-09 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2019185680A1 (en) 2018-03-28 2019-10-03 Dsm Ip Assets B.V. Enzyme composition
WO2019185681A1 (en) 2018-03-28 2019-10-03 Dsm Ip Assets B.V. Enzyme composition
US11608364B2 (en) 2018-04-30 2023-03-21 Dsm Ip Assets B.V. Steviol glycoside transport
WO2019219804A1 (en) 2018-05-17 2019-11-21 Dsm Ip Assets B.V. Process for producing a polypeptide
EP3797159A1 (en) * 2018-05-22 2021-03-31 DSM IP Assets B.V. Modified sterol acyltransferases
PL3802843T3 (pl) 2018-05-30 2023-06-05 Versalis S.P.A. Sposób wytwarzania cukrów z materiałów węglowodanowych
WO2020058248A1 (en) 2018-09-18 2020-03-26 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
WO2020058249A1 (en) 2018-09-18 2020-03-26 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
WO2020058253A1 (en) 2018-09-18 2020-03-26 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
BR112021007723A2 (pt) * 2018-10-23 2021-08-10 MARA Renewables Corporation métodos de produção de micro-organismos com consumo aumentado de xilose, de crescimento de micro-organismos isolados e para reduzir a produção de xilitol em culturas, e, população de micro-organismos isolados
CN109593662B (zh) * 2018-12-11 2021-12-03 大连理工大学 一种乙酸和木糖共利用的马克斯克鲁维酵母菌株及筛选方法
CN109504695B (zh) * 2019-01-02 2022-08-09 中国农业科学院作物科学研究所 多中心单鞭毛菌木糖异构酶基因及其应用
US12157906B2 (en) 2019-03-12 2024-12-03 Versalis S.P.A. Process for producing a fermentation broth
US20220340943A1 (en) 2019-09-10 2022-10-27 Dsm Ip Assets B.V. Enzyme composition
US20220380813A1 (en) 2019-11-08 2022-12-01 Dsm Ip Assets B.V. Process for producing ethanol
CA3198626A1 (en) 2020-10-22 2022-04-28 Dsm Ip Assets B.V. Microorganisms for diterpene production
BR112023020370A2 (pt) 2021-04-06 2023-11-21 Dsm Ip Assets Bv Composição de enzimas
US20240218410A1 (en) 2021-04-06 2024-07-04 Dsm Ip Assets B.V. Enzyme composition
CA3214435A1 (en) 2021-04-06 2022-10-13 Dsm Ip Assets B.V. Enzyme composition
EP4320259A1 (en) 2021-04-08 2024-02-14 Versalis S.p.A. Process for the preparation of a sugar product and a fermentation product
CN115851850B (zh) * 2021-09-23 2025-11-28 中国科学院天津工业生物技术研究所 利用二氧化碳制备淀粉的方法、重组微生物和构建重组微生物的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10117771A (ja) * 1996-10-23 1998-05-12 Natl Food Res Inst 冷凍生地耐性実用パン酵母
US6566107B1 (en) * 2000-11-01 2003-05-20 Midwest Research Institute Recombinant Zymomonas mobilis with improved xylose utilization
BR0306740A (pt) * 2002-01-23 2004-12-28 Royal Nedalco B V Célula hospedeira transformada com um construto de ácido nucléico, molécula de ácido nucleico isolada, e, processos para a produção de etanol, e de um produto de fermentação
ZA200509697B (en) * 2003-05-02 2007-03-28 Cargill Dow Llc Genetically modified yeast species and fermentation processes using genetically modified yeast
US8034591B2 (en) * 2004-07-16 2011-10-11 Techische Universiteit Delft Xylose-fermenting yeast engineered to increase flux of the pentose phosphate pathway

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