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DK1747290T3 - Anvendelse af fuldblod i PCR reaktioner - Google Patents

Anvendelse af fuldblod i PCR reaktioner

Info

Publication number
DK1747290T3
DK1747290T3 DK05759554.8T DK05759554T DK1747290T3 DK 1747290 T3 DK1747290 T3 DK 1747290T3 DK 05759554 T DK05759554 T DK 05759554T DK 1747290 T3 DK1747290 T3 DK 1747290T3
Authority
DK
Denmark
Prior art keywords
whole blood
pcr reactions
pcr
reactions
blood
Prior art date
Application number
DK05759554.8T
Other languages
English (en)
Inventor
Wayne M Barnes
Milko B Kermekchiev
Original Assignee
Dna Polymerase Technology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/850,816 external-priority patent/US20050260606A1/en
Application filed by Dna Polymerase Technology Inc filed Critical Dna Polymerase Technology Inc
Application granted granted Critical
Publication of DK1747290T3 publication Critical patent/DK1747290T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DK05759554.8T 2004-05-20 2005-05-20 Anvendelse af fuldblod i PCR reaktioner DK1747290T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10/850,816 US20050260606A1 (en) 2004-05-20 2004-05-20 Use of whole blood in PCR reactions
US11/005,559 US7462475B2 (en) 2004-05-20 2004-12-06 Use of whole blood in PCR reactions
PCT/US2005/017669 WO2005113829A2 (en) 2004-05-20 2005-05-20 Use of whole blood in pcr reactions

Publications (1)

Publication Number Publication Date
DK1747290T3 true DK1747290T3 (da) 2014-08-11

Family

ID=35428948

Family Applications (1)

Application Number Title Priority Date Filing Date
DK05759554.8T DK1747290T3 (da) 2004-05-20 2005-05-20 Anvendelse af fuldblod i PCR reaktioner

Country Status (7)

Country Link
US (3) US7462475B2 (da)
EP (1) EP1747290B1 (da)
JP (1) JP2008506417A (da)
AU (1) AU2005245948B2 (da)
CA (1) CA2567620C (da)
DK (1) DK1747290T3 (da)
WO (1) WO2005113829A2 (da)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7462475B2 (en) 2004-05-20 2008-12-09 Dna Poleymerase Technology, Inc. Use of whole blood in PCR reactions
US8628918B2 (en) * 2005-05-09 2014-01-14 Affymetrix, Inc. Multiplex capture of nucleic acids
US8632970B2 (en) 2005-05-09 2014-01-21 Affymetrix, Inc. Multiplex capture of nucleic acids
EP1880025B1 (en) * 2005-05-12 2011-03-16 Affymetrix, Inc. Multiplex branched-chain dna assays
WO2007001986A2 (en) * 2005-06-20 2007-01-04 Yuling Luo Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
US7927798B2 (en) * 2005-10-05 2011-04-19 Panomics, Inc. Detection of nucleic acids from whole blood
WO2008034110A2 (en) * 2006-09-14 2008-03-20 Dna Polymerase Technology Inc. Use of taq polymerase mutant enzymes for dna amplification in the presence of pcr inhibitors
JP5292722B2 (ja) * 2007-05-09 2013-09-18 凸版印刷株式会社 未精製血液をサンプルとする核酸増幅法
EP2297343A1 (en) * 2008-05-16 2011-03-23 New England Biolabs, Inc. Enzyme reagents for amplification of polynucleotides in the presence of inhibitors
FI20095729A0 (fi) * 2009-06-26 2009-06-26 Finnzymes Oy Menetelmä deoksiribonukleiinihappojen kvantitatiiviseen PCR-monistukseen PCR-inhibiittoreita sisältävästä näytteestä
ES2648564T3 (es) 2010-10-21 2018-01-04 Advanced Cell Diagnostics, Inc. Método ultrasensible para la detección in situ de ácidos nucleicos
US8409807B2 (en) 2010-10-22 2013-04-02 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
BR112013010952B1 (pt) 2010-10-22 2020-08-25 T2 Biosystems, Inc. métodos para detectar a presença de um analito de ácido nucleico e uma espécie de candida em uma amostra líquida, para detectar a presença de um patógeno, um vírus e um ácido nucleico alvo em uma amostra de sangue total, e para amplificação de um ácido nucleico de patógeno alvo em uma amostra de sangue total, bem como sistema para a detecção de um ou mais analitos e cartucho removível dimensionado para facilitar inserção e remoção de um sistema
US8563298B2 (en) 2010-10-22 2013-10-22 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
ES2561885T3 (es) * 2011-04-11 2016-03-01 F. Hoffmann-La Roche Ag ADN polimerasas de actividad mejorada
GB201113430D0 (en) 2011-08-03 2011-09-21 Fermentas Uab DNA polymerases
US11208636B2 (en) 2011-08-10 2021-12-28 Life Technologies Corporation Polymerase compositions, methods of making and using same
LT2742151T (lt) 2011-08-10 2018-02-12 Life Technologies Corporation Polimerazės kompozicija
WO2013083264A1 (en) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity
US9562271B2 (en) 2012-04-20 2017-02-07 T2 Biosystems, Inc. Compositions and methods for detection of Candida species
WO2014062848A1 (en) 2012-10-16 2014-04-24 Dna Polymerase Technology, Inc. Inhibition-resistant polymerases
GB201219137D0 (en) * 2012-10-24 2012-12-05 Ge Healthcare Uk Ltd Direct nucleic acid amplification kit, reagent and method
WO2016118766A2 (en) 2015-01-21 2016-07-28 T2 Biosystems, Inc. Nmr methods and systems for the rapid detection of tick-borne pathogens
US11091745B2 (en) 2015-05-12 2021-08-17 Dna Polymerase Technology, Inc. Mutant polymerases and uses thereof
US11078528B2 (en) 2015-10-12 2021-08-03 Advanced Cell Diagnostics, Inc. In situ detection of nucleotide variants in high noise samples, and compositions and methods related thereto
JP6963238B2 (ja) * 2015-11-27 2021-11-05 国立大学法人九州大学 Ｄｎａポリメラーゼ変異体
CA3011991A1 (en) 2016-01-21 2017-07-27 T2 Biosystems, Inc. Rapid antimicrobial susceptibility testing using high-sensitivity direct detection methods
CA3011901A1 (en) 2016-01-21 2017-07-27 T2 Biosystems, Inc. Nmr methods and systems for the rapid detection of bacteria
US12077810B2 (en) 2016-04-14 2024-09-03 T2 Biosystems, Inc. Methods and systems for amplification in complex samples
US10626444B2 (en) * 2016-10-14 2020-04-21 ACUDX Inc. Methods of assaying nucleic acids of low quantities using a buffer nucleic acid
JP6730525B2 (ja) 2016-11-21 2020-07-29 ナノストリング テクノロジーズ，インコーポレイティド 化学組成物とそれを利用する方法
EP3740591A4 (en) 2018-01-19 2022-02-23 Bio-Rad Laboratories, Inc. Mutant dna polymerases
EP3794146B1 (en) 2018-05-14 2025-12-10 Bruker Spatial Biology, Inc. Method for identifying a predetermined nucleotide sequence
CN109022386B (zh) * 2018-07-12 2020-11-03 深圳市华中生物药械有限公司 重组Taq直扩酶及其制备方法、重组质粒和工程菌
GB2587177A (en) * 2019-01-09 2021-03-24 3Cr Bioscience Ltd Polymerase
US20220090194A1 (en) * 2019-01-09 2022-03-24 3Cr Bioscience Ltd. Method for detecting a nucleic acid sequence
CN110295237B (zh) * 2019-06-27 2023-07-25 河北农业大学 一种改进的鸡隐性白羽基因型的分子鉴定方法
CN115803434A (zh) * 2020-05-29 2023-03-14 基诺泰科有限公司 具有提高的对基因变异的辨别性的dna聚合酶变体
CN114134220A (zh) * 2022-01-30 2022-03-04 济凡生物科技（北京）有限公司 一种可以用于血液检测的pcr反应液及其试剂盒

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5466591A (en) * 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
DE553264T1 (de) * 1990-10-05 1994-04-28 Wayne M Barnes Thermostabile dna polymerase.
EP0590327B1 (en) * 1992-09-11 2003-04-09 F. Hoffmann-La Roche Ag Detection of nucleic acids in blood
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US6818431B1 (en) 1995-10-18 2004-11-16 Shanghai Mendel Dna Center Co. Ltd. DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
WO1999054455A1 (fr) * 1998-04-23 1999-10-28 Takara Shuzo Co., Ltd. Procede de synthese de l'adn
US6214557B1 (en) * 2000-06-06 2001-04-10 Washington University Cold sensitive mutant DNA polymerases
US6428962B1 (en) * 2001-02-12 2002-08-06 Dna Analysis, Inc. Nucleic acid collection barrier method and apparatus
US6896872B2 (en) 2001-06-27 2005-05-24 Svetlana A. Dambinova Rapid multiple panel of biomarkers in laboratory blood tests for TIA/stroke
WO2004013279A2 (en) * 2002-05-14 2004-02-12 Fidelity Systems, Inc. Helix-hairpin-helix motifs to manipulate properties of dna processing enzymes
US7462475B2 (en) 2004-05-20 2008-12-09 Dna Poleymerase Technology, Inc. Use of whole blood in PCR reactions
WO2008034110A2 (en) 2006-09-14 2008-03-20 Dna Polymerase Technology Inc. Use of taq polymerase mutant enzymes for dna amplification in the presence of pcr inhibitors

Also Published As

Publication number Publication date
EP1747290B1 (en) 2014-05-07
US20060084074A1 (en) 2006-04-20
WO2005113829A2 (en) 2005-12-01
US7462475B2 (en) 2008-12-09
US8470563B2 (en) 2013-06-25
AU2005245948B2 (en) 2011-04-07
CA2567620C (en) 2016-09-20
EP1747290A4 (en) 2009-01-14
JP2008506417A (ja) 2008-03-06
WO2005113829A3 (en) 2008-04-03
US20130252309A1 (en) 2013-09-26
AU2005245948A1 (en) 2005-12-01
CA2567620A1 (en) 2005-12-01
EP1747290A2 (en) 2007-01-31
US20090170060A1 (en) 2009-07-02

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