DK1618183T3 - Ny bacillus-cellulase 029cel - Google Patents
Ny bacillus-cellulase 029cel Download PDFInfo
- Publication number
- DK1618183T3 DK1618183T3 DK04750928.6T DK04750928T DK1618183T3 DK 1618183 T3 DK1618183 T3 DK 1618183T3 DK 04750928 T DK04750928 T DK 04750928T DK 1618183 T3 DK1618183 T3 DK 1618183T3
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- DK
- Denmark
- Prior art keywords
- cellulase
- acid sequence
- amino acid
- sequence
- polypeptide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
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Claims (31)
1. Isoleret polynucleotid valgt fra gruppen bestående af: (a) en nucleinsyresekvens med mindst 85% sekvensidentitet til SEQ ID NO: 1 eller komplementet heraf. (b) en nucleinsyresekvens, som koder for eller er komplementær til en sekvens, som koder for et 029cel-polypeptid med mindst 85% sekvensidentitet til aminosyresekvensen SEQ ID NO: 3, (c) en nucleinsyresekvens, som koder for eller er komplementær til en sekvens, som koder for et 029cel-polypeptid med mindst 90% sekvensidentitet til aminosyresekvensen SEQ ID NO: 3, (d) en nucleinsyresekvens, som koder for eller er komplementær til en sekvens, som koder for et 029cel-polypeptid med mindst 95% sekvensidentitet til aminosyresekvensen SEQ ID NO: 3, (e) en nucleinsyresekvens, som koder for eller er komplementær til en sekvens, som koder for et 029cel-polypeptid med aminosyresekvensen SEQ ID NO: 3, hvor nævnte isolerede polynucleotid koder for et polypeptid med biologisk aktivitet af en cellulase, og hvor identiteten bestemmes ved CLUSTAL-W-program i MacVector version 6,5 betjent med default parametre indbefattet en ’’open gap penalty” på 10,0, en ’’excented gap penalty” på 0,1 og en BLOSUM 30 similari-tetsmatrix.
2. Isoleret polynucleotid valgt fra gruppen bestående af: (a) en nucleinsyresekvens SEQ ID NO: 1 eller komplementet hertil, (b) en nucleinsyresekvens, som hybridiserer under højstringente betingelser til sekvensen SEQ ID NO: 1 eller komplementet hertil, (c) en nucleinsyresekvens SEQ ID NO: 2 eller komplementet hertil, og (d) en nucleinsyresekvens, som hybridiserer ved højstringente betingelser til sekvensen SEQ ID NO: 2 eller komplementet heraf, 46 hvori nævnte isolerede polynucleotid koder for et polypeptid med den biologiske aktivitet af en cellulase, og hvor hybridisering udføres ved 42°C i 50% formamid, 6 X SCC, 5X Denhardts opløsning, 0,5% SDS og 100 pg/ml denatureret bærer-DNA efterfulgt afvask to gange i 2X SSPE og 0,5% SDS ved stuetemperatur og to yderligere gange i 0,1 SSPE og 0,5% SDS ved 42°C.
3. Isoleret polynucleotid ifølge krav 1, hvor nucleotidet er valgt fra gruppen mRNA, DNA, cDNA, genomisk DNA og en antisense analog heraf.
4. Isoleret polynucleotid ifølge krav 3, hvor nævnte polynucleotid er et RNA-molekyle.
5. Isoleret polynucleotid ifølge krav 1, som koder for et enzym med cellulase-aktivitet, hvor enzymet er isoleret fra en trichodermakilde.
6. Isoleret polynucleotid ifølge krav 5, hvor enzymet er isoleret fra trichoder-ma reesei.
7. Ekspressionskonstruktion omfattende en polynucleotidsekvens, som koder for en aminosyresekvens, som har cellulaseaktivitet og (i) har mindst 85% sekvensidentitet med aminosyresekvensen SEQ ID NO: 3, eller (ii) er komplementært til en nucleotidsekvens med mindst 85% sekvensidentitet til en nucleotidsekvens, som koder for aminosyresekvensen SEQ ID NO: 3, hvor identiteten bestemmes ved CLUSTAL-W program i MacVector version 6,5 betjent med default parametre indbefattet en ’’open gap penalty” på 10,0, en ’’extended gap penalty” på 0,1 og en BLOSUM 30 similaritetsmatrix.
8. Ekspressionsvektor omfattende polynucleotidet ifølge krav 1. 47
9. Ekspressionsvektor omfattende et isoleret polynucleotid ifølge krav 1 operativt forbundet til kontrolsekvenser genkendt af en værtscelle transformeret med vektoren.
10. Ekspressionsvektor ifølge krav 9 omfattende en regulatorisk polynucleo-tidsekvens indbefatter en promotorsekvens stammende fra et glucoseisomera-segen fra actinoplanes, en signalsekvens stammende fra et Streptomyces cellu-lasegen og en polynucleotidsekvens, som koder for en 029cel cellulase.
11. Vektor omfattende ekspressionskonstruktionen i krav 7,
12. Værtscelle transformeret med vektoren i krav 8.
13. Værtscelle ifølge krav 12, som er en prokaryotisk celle.
14. Værtscelle ifølge krav 12, som er en eukaryotisk celle.
15. I al væsentlighed oprenset 029cel-polypeptid med den biologiske aktivitet af en cellulase omfattende en sekvens valgt fra gruppen bestående af: (a) en aminosyresekvens med mindst 85% sekvensidentitet til aminosyre-sekvensen SEQ ID NO: 3, (b) en aminosyresekvens med mindst 90% sekvensidentitet til aminosyre-sekvensen SEQ ID NO: 3, (c) en aminosyresekvens med mindst 95% sekvensidentitet til aminosyre-sekvensen SEQ ID NO: 3, (d) en aminosyresekvens SEQ ID NO: 3, hvor identiteten er bestemt med CLUSTAL-W programmet i MacVector version 6,5, betjent med default-værdier indbefattet ’’open gap penalty” på 10,0, ’’extended gap penalty” på 0,1 og en BLOSUM 30 similaritetsmatrix. 48
16. I al væsentlighed oprenset cellulasepolypeptid ifølge krav 15 eller et derivat, som er opnåeligt fra en Bacillus.
17. Fremgangsmåde til fremstilling af en cellulase omfattende trinnene: (a) dyrkning af værtscellen ifølge krav 12 i et passende dyrkningsmedie under egnede betingelser for at producere cellulasen, (b) opnåelse af nævnte producerede cellulase.
18. Fremgangsmåde ifølge krav 17, hvor værtscellen er en filamentøs svampeeller gærcelle.
19. Fremgangsmåde ifølge krav 17, hvor værtscellen er en bakterie.
20. Fremgangsmåde ifølge krav 19, hvor bakterien er en Streptomyces.
21. Oprenset enzym med cellulaseaktivitet fremstillet ved fremgangsmåden ifølge krav 17.
22. Antisense-oligonucleotid komplementært til en messenger RNA, som koder for et polypeptid med sekvensen SEQ ID NO: 3, hvori ved eksponering til en cellulaseproducerende værtscelle, nævnte oligonucleotid mindsker eller inhibe-rer produktionen af cellulase fra nævnte værtscelle.
23. Antisense-oligonucleotid ifølge krav 22, hvor værtscellen er en filamentøs svamp.
24. Detergentsammensætning, hvor nævnte sammensætning omfatter et overfladeaktivt stof og et polypeptid valgt fra gruppen bestående af: (a) en aminosyresekvens med mindst 85% sekvensidentitet til aminosyre-sekvensen SEQ ID NO: 3, 49 (b) en aminosyresekvens med mindst 90% sekvensidentitet til aminosyre-sekvensen SEQ ID NO: 3, (c) en aminosyresekvens med mindst 95% sekvensidentitet til aminosyre-sekvensen SEQ ID NO: 3, (d) en aminosyresekvens SEQ ID NO: 3, hvori identiteten bestemmes med CLUSTAL-W program i MacVector version 6,5 betjent med defaultværdier indbefattet en ’’open gap penalty” på 10,0, en ’’extended gap penalty” på 0,1 og en BLOSUM 30 similaritetsmatrix.
25. Detergent ifølge krav 24, hvor nævnte detergent er et vasketøjsdetergent.
26. Detergent ifølge krav 24, hvor nævnte detergent er et opvaskedetergent.
27. Foderadditiv omfattende en cellulase ifølge krav 15.
28. Fremgangsmåde til behandling af træpulp omfattende at bringe nævnte træpulp i kontakt med en cellulase ifølge krav 15.
29. Fremgangsmåde til omdannelse af biomasse til sukre omfattende at bringe nævnte biomasse i kontakt med en cellulase ifølge krav 15.
30. Fremgangsmåde ifølge krav 29, som yderligere omfatter opnåelsen af majssirup med højtfructoseindhold.
31. Fremgangsmåde til at fremstillet ethanol, hvilken fremgangsmåde omfatter trinnene: (a) at bringe en biomassesammensætning i kontakt med en enzymatisk sammensætning omfattende 029cel ifølge krav 15 til at give en sukkeropløsning, (b) tilsætning til sukkeropløsningen afen fermentativ mikroorganisme, og 50 (c) dyrkning af den fermentative mikroorganisme ved betingelser tilstrækkelige til at producere ethanol.
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| US46683103P | 2003-04-29 | 2003-04-29 | |
| PCT/US2004/013258 WO2004097001A2 (en) | 2003-04-29 | 2004-04-28 | Novel bacillus 029cel cellulase |
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| DK1618183T3 true DK1618183T3 (da) | 2015-02-23 |
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| CA (1) | CA2523328C (da) |
| DK (1) | DK1618183T3 (da) |
| ES (1) | ES2530518T3 (da) |
| WO (1) | WO2004097001A2 (da) |
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| SE535469C2 (sv) | 2010-11-29 | 2012-08-21 | Rational Enzyme Mining Rem Ab | Rationell enzymvaskning |
| WO2013181647A2 (en) | 2012-06-01 | 2013-12-05 | Danisco Us Inc. | Compositions and methods of producing isoprene and/or industrrial bio-products using anaerobic microorganisms |
| US9850512B2 (en) | 2013-03-15 | 2017-12-26 | The Research Foundation For The State University Of New York | Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield |
| CA2915980A1 (en) | 2013-06-21 | 2014-12-24 | Danisco Us Inc. | Compositions and methods for clostridial transformation |
| BR112016013684A2 (pt) | 2013-12-16 | 2017-08-08 | Du Pont | Solução aquosa ou hidrocoloide, método para aumentar a viscosidade de uma composição e método para o tratamento de um material |
| KR102410391B1 (ko) | 2013-12-18 | 2022-06-16 | 뉴트리션 앤드 바이오사이언시스 유에스에이 4, 인크. | 양이온성 폴리 알파-1,3-글루칸 에테르 |
| WO2015123323A1 (en) | 2014-02-14 | 2015-08-20 | E. I. Du Pont De Nemours And Company | Poly-alpha-1,3-1,6-glucans for viscosity modification |
| CA2937830A1 (en) | 2014-03-11 | 2015-09-17 | E. I. Du Pont De Nemours And Company | Oxidized poly alpha-1,3-glucan as detergent builder |
| US9951363B2 (en) | 2014-03-14 | 2018-04-24 | The Research Foundation for the State University of New York College of Environmental Science and Forestry | Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects |
| US9714403B2 (en) | 2014-06-19 | 2017-07-25 | E I Du Pont De Nemours And Company | Compositions containing one or more poly alpha-1,3-glucan ether compounds |
| WO2015195777A1 (en) | 2014-06-19 | 2015-12-23 | E. I. Du Pont De Nemours And Company | Compositions containing one or more poly alpha-1,3-glucan ether compounds |
| WO2016106013A1 (en) | 2014-12-23 | 2016-06-30 | E. I. Du Pont De Nemours And Company | Enzymatically produced cellulose |
| JP2018511684A (ja) | 2015-04-03 | 2018-04-26 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ | ゲル化デキストランエーテル |
| EP3374488B1 (en) | 2015-11-13 | 2020-10-14 | DuPont Industrial Biosciences USA, LLC | Glucan fiber compositions for use in laundry care and fabric care |
| EP3374400B1 (en) | 2015-11-13 | 2022-04-13 | Nutrition & Biosciences USA 4, Inc. | Glucan fiber compositions for use in laundry care and fabric care |
| JP2019504932A (ja) | 2015-11-13 | 2019-02-21 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ | 洗濯ケアおよび織物ケアにおいて使用するためのグルカン繊維組成物 |
| WO2017091533A1 (en) | 2015-11-26 | 2017-06-01 | E. I. Du Pont De Nemours And Company | Polypeptides capable of producing glucans having alpha-1,2 branches and use of the same |
| WO2017152169A1 (en) | 2016-03-04 | 2017-09-08 | Danisco Us Inc. | Engineered ribosomal promoters for protein production in microorganisms |
| WO2019089898A1 (en) | 2017-11-02 | 2019-05-09 | Danisco Us Inc | Freezing point depressed solid matrix compositions for melt granulation of enzymes |
| EP3707168B1 (en) | 2017-11-10 | 2022-03-30 | Nutrition & Biosciences USA 4, Inc. | Unique morphological polysaccharide |
| CN109321503A (zh) * | 2018-11-05 | 2019-02-12 | 南京农业大学 | 一种促进木薯渣基质发酵进程的放线菌的分离方法 |
| WO2024200535A1 (en) | 2023-03-27 | 2024-10-03 | Dsm Ip Assets B.V. | Endoprotease composition |
| EP4437856A1 (en) | 2023-03-31 | 2024-10-02 | DSM IP Assets B.V. | Novel enzyme composition and novel beer brewing process |
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| GB1368599A (en) | 1970-09-29 | 1974-10-02 | Unilever Ltd | Softening compositions |
| US4411994A (en) | 1978-06-08 | 1983-10-25 | The President And Fellows Of Harvard College | Protein synthesis |
| US4338397A (en) | 1980-04-11 | 1982-07-06 | President And Fellows Of Harvard College | Mature protein synthesis |
| GB2094826B (en) | 1981-03-05 | 1985-06-12 | Kao Corp | Cellulase enzyme detergent composition |
| GB2095275B (en) | 1981-03-05 | 1985-08-07 | Kao Corp | Enzyme detergent composition |
| US4513086A (en) | 1981-10-15 | 1985-04-23 | Eli Lilly And Company | Cloning vectors for use in streptomyces and related organisms |
| US4513085A (en) | 1982-10-07 | 1985-04-23 | Eli Lilly And Company | Functionally independent cloning vectors for use in streptomyces |
| US5264366A (en) | 1984-05-29 | 1993-11-23 | Genencor, Inc. | Protease deficient bacillus |
| US4745056A (en) | 1984-10-23 | 1988-05-17 | Biotechnica International, Inc. | Streptomyces secretion vector |
| US5364770A (en) | 1985-08-29 | 1994-11-15 | Genencor International Inc. | Heterologous polypeptides expressed in aspergillus |
| DK163591C (da) | 1985-10-08 | 1992-08-24 | Novo Nordisk As | Fremgangsmaade til behandling af et tekstilstof med en cellulase |
| US5514590A (en) | 1985-11-06 | 1996-05-07 | Cangene Corporation | Expression system comprising DNA encoding the signal peptide of protease B from Streptomyces griseus |
| JP2530181B2 (ja) * | 1986-10-31 | 1996-09-04 | 花王株式会社 | アルカリセルラ―ゼ遺伝子を含むｄｎａ断片並びに該ｄｎａ断片を組み込んだ組換えプラスミド及び組換え微生物 |
| AU1299988A (en) | 1987-03-09 | 1988-10-10 | Cetus Corporation | Expression of heterologous genes in streptomyces species |
| FI87372C (fi) | 1989-03-30 | 1992-12-28 | Genencor Int Europ | Foerfarande foer framstaellning av fluffmassa med foerbaettrad rivbarhet |
| JPH03251174A (ja) * | 1990-02-28 | 1991-11-08 | Oji Paper Co Ltd | セルラーゼａｅ―１、セルラーゼａｅ―１遺伝子を含むｄｎａ断片及びセルラーゼａｅ―１の製造方法 |
| US5254283A (en) | 1991-01-17 | 1993-10-19 | Genencor International, Inc. | Isophthalic polymer coated particles |
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| US5622866A (en) | 1994-06-23 | 1997-04-22 | Merck & Co., Inc. | Expression cassettes useful in construction of integrative and replicative expression vectors for Streptomyces |
| US6255115B1 (en) | 1997-04-07 | 2001-07-03 | Unilever Patent Holdings Bv | Agrobacterium mediated transformation of moulds, in particular those belonging to the genus Aspergillus |
| US6287839B1 (en) * | 1997-11-19 | 2001-09-11 | Genencor International, Inc. | Cellulase producing actinomycetes, cellulase produced therefrom and method of producing same |
| DK1038008T3 (da) * | 1997-12-16 | 2007-03-12 | Genencor Int | Fremgangsmåde til fremstilling af EGIII-lignende enzymer |
| EP2305820A1 (en) * | 2002-06-14 | 2011-04-06 | Verenium Corporation | Xylanases, nucleic acids encoding them and methods for making and using them |
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- 2004-04-28 WO PCT/US2004/013258 patent/WO2004097001A2/en not_active Ceased
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| Publication number | Publication date |
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| WO2004097001A2 (en) | 2004-11-11 |
| JP4629664B2 (ja) | 2011-02-09 |
| EP1618183A2 (en) | 2006-01-25 |
| EP1618183A4 (en) | 2010-04-07 |
| CN101410520B (zh) | 2012-05-30 |
| US20100048417A1 (en) | 2010-02-25 |
| CA2523328C (en) | 2012-07-03 |
| US20070172916A1 (en) | 2007-07-26 |
| EP1618183B1 (en) | 2014-11-19 |
| ES2530518T3 (es) | 2015-03-03 |
| CA2523328A1 (en) | 2004-11-11 |
| US8158397B2 (en) | 2012-04-17 |
| US7604974B2 (en) | 2009-10-20 |
| JP2007525181A (ja) | 2007-09-06 |
| CN101410520A (zh) | 2009-04-15 |
| WO2004097001A3 (en) | 2009-05-14 |
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