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DK1495119T3 - Process and automaton for extracting nucleic acids from a complex mixture - Google Patents

Process and automaton for extracting nucleic acids from a complex mixture

Info

Publication number
DK1495119T3
DK1495119T3 DK03745840T DK03745840T DK1495119T3 DK 1495119 T3 DK1495119 T3 DK 1495119T3 DK 03745840 T DK03745840 T DK 03745840T DK 03745840 T DK03745840 T DK 03745840T DK 1495119 T3 DK1495119 T3 DK 1495119T3
Authority
DK
Denmark
Prior art keywords
nucleic acids
complex mixture
automaton
extracting nucleic
microorganisms
Prior art date
Application number
DK03745840T
Other languages
Danish (da)
Inventor
Gabriel Festoc
Sylvie Hallier-Soulier
Gaelle Leborgne
Nadine Batisse-Debitte
Xavier Badiche
Original Assignee
Genesystems
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genesystems filed Critical Genesystems
Application granted granted Critical
Publication of DK1495119T3 publication Critical patent/DK1495119T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification

Landscapes

  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to a method for extracting nucleic acids of microorganisms contained in a complex mixture, particularly a food mixture, comprising at least the following steps: the mixture (10) is clarified; the microorganisms are retained on a filtering cartridge (12); said microorganisms are lysed (14); the nucleic acids are recovered (16), concentrated, and purified (18). The invention also relates to a filtering cartridge and an apparatus used for carrying out the inventive extraction method. Also disclosed is a device for extracting, detecting, and if necessary quantifying the nucleic acids of microorganisms contained in a complex mixture.
DK03745840T 2002-04-09 2003-04-09 Process and automaton for extracting nucleic acids from a complex mixture DK1495119T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0204424A FR2838066B1 (en) 2002-04-09 2002-04-09 METHOD AND AUTOMATE FOR EXTRACTING NUCLEIC ACIDS FROM A COMPLEX MIXTURE
PCT/FR2003/001123 WO2003085109A2 (en) 2002-04-09 2003-04-09 Method and apparatus for extracting nucleic acids from a complex mixture

Publications (1)

Publication Number Publication Date
DK1495119T3 true DK1495119T3 (en) 2007-05-21

Family

ID=28052243

Family Applications (1)

Application Number Title Priority Date Filing Date
DK03745840T DK1495119T3 (en) 2002-04-09 2003-04-09 Process and automaton for extracting nucleic acids from a complex mixture

Country Status (10)

Country Link
EP (1) EP1495119B1 (en)
JP (1) JP2005526509A (en)
AT (1) ATE351903T1 (en)
AU (1) AU2003246804A1 (en)
DE (1) DE60311244T2 (en)
DK (1) DK1495119T3 (en)
ES (1) ES2279960T3 (en)
FR (1) FR2838066B1 (en)
PT (1) PT1495119E (en)
WO (1) WO2003085109A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2829500B1 (en) 2001-09-13 2003-12-12 Hemosystem PROCESS FOR THE CONCENTRATION AND DETECTION OF PATHOGENIC SPROUTS FROM BLOOD PRODUCTS AND / OR DERIVATIVES THEREOF AND DEVICE FOR CARRYING OUT SAID METHOD
US20050250138A1 (en) 2004-05-04 2005-11-10 Millipore Corporation Process for the enumeration and identification of microorganisms
JP4195039B2 (en) * 2006-02-16 2008-12-10 善之 辻本 Sample pretreatment method and reagent
FR2901281B1 (en) * 2006-05-19 2008-08-15 Hemosystem Sa PROCESS FOR EXTRACTING DESOXYRIBONUCLEIC ACIDS (DNA) FROM MICROORGANISMS WHICH MAY BE PRESENT IN A BLOOD SAMPLE
JP2008005781A (en) * 2006-06-29 2008-01-17 Olympus Corp Specimen-treating method
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
WO2010111265A1 (en) 2009-03-24 2010-09-30 University Of Chicago Slip chip device and methods
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
KR102458821B1 (en) * 2015-12-29 2022-10-25 국민대학교산학협력단 A method for rapidly detecting microorganism using pcr product

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0245945B1 (en) * 1986-04-11 1993-11-10 Applied Biosystems, Inc. Automated nucleic acid extractor
US5187083A (en) * 1990-11-13 1993-02-16 Specialty Laboratories, Inc. Rapid purification of DNA
FR2678950B1 (en) * 1991-07-09 1993-11-05 Bertin Et Cie CARTRIDGE, DEVICE AND METHOD FOR EXTRACTING NUCLEIC ACIDS SUCH AS DNA FROM A SAMPLE OF BLOOD OR TISSUE CELLS.
WO1994021780A1 (en) * 1993-03-18 1994-09-29 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture Apparatus for the quantitative determination of particulate analytes
FR2712511B1 (en) * 1993-11-16 1996-02-09 Bertin & Cie Cartridge for the preparation of purified nucleic acids.
AU1752799A (en) * 1997-08-27 1999-04-05 Anda Biologicals S.A. Rapid filter diagnostic device and method for the direct detection of micro-organisms in body fluids
EP1119576B1 (en) * 1998-10-09 2003-06-11 Whatman Bioscience Limited Isolation method for nucleic acid and apparatus
US6958392B2 (en) * 1998-10-09 2005-10-25 Whatman, Inc. Methods for the isolation of nucleic acids and for quantitative DNA extraction and detection for leukocyte evaluation in blood products

Also Published As

Publication number Publication date
JP2005526509A (en) 2005-09-08
WO2003085109A3 (en) 2004-04-01
FR2838066B1 (en) 2004-06-25
ES2279960T3 (en) 2007-09-01
AU2003246804A1 (en) 2003-10-20
ATE351903T1 (en) 2007-02-15
EP1495119A2 (en) 2005-01-12
PT1495119E (en) 2007-04-30
WO2003085109A2 (en) 2003-10-16
DE60311244D1 (en) 2007-03-08
DE60311244T2 (en) 2007-11-15
FR2838066A1 (en) 2003-10-10
EP1495119B1 (en) 2007-01-17

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