[go: up one dir, main page]

DK1356037T3 - Bibliotek med en samling af celler - Google Patents

Bibliotek med en samling af celler

Info

Publication number
DK1356037T3
DK1356037T3 DK02709979.5T DK02709979T DK1356037T3 DK 1356037 T3 DK1356037 T3 DK 1356037T3 DK 02709979 T DK02709979 T DK 02709979T DK 1356037 T3 DK1356037 T3 DK 1356037T3
Authority
DK
Denmark
Prior art keywords
novel
cells
libraries
host cells
library
Prior art date
Application number
DK02709979.5T
Other languages
English (en)
Inventor
Neil Goldsmith
Alexandra M P Sorensen
Soren V S Nielsen
Original Assignee
Evolva Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva Ltd filed Critical Evolva Ltd
Application granted granted Critical
Publication of DK1356037T3 publication Critical patent/DK1356037T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Aerials With Secondary Devices (AREA)
  • Eye Examination Apparatus (AREA)
  • Holo Graphy (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DK02709979.5T 2001-01-25 2002-01-25 Bibliotek med en samling af celler DK1356037T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA200100128 2001-01-25
DKPA200100679 2001-05-01
US30086301P 2001-06-27 2001-06-27
PCT/DK2002/000056 WO2002059297A2 (en) 2001-01-25 2002-01-25 A library of a collection of cells

Publications (1)

Publication Number Publication Date
DK1356037T3 true DK1356037T3 (da) 2011-06-27

Family

ID=43646216

Family Applications (1)

Application Number Title Priority Date Filing Date
DK02709979.5T DK1356037T3 (da) 2001-01-25 2002-01-25 Bibliotek med en samling af celler

Country Status (9)

Country Link
US (2) US7838287B2 (da)
EP (1) EP1356037B1 (da)
JP (2) JP2004525624A (da)
AT (1) ATE501251T1 (da)
AU (1) AU2002227883A1 (da)
CA (1) CA2474172C (da)
DE (1) DE60239386D1 (da)
DK (1) DK1356037T3 (da)
WO (1) WO2002059297A2 (da)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
DE60239386D1 (de) 2001-01-25 2011-04-21 Evolva Ltd Zellbibliothek
JP4472345B2 (ja) * 2002-01-25 2010-06-02 エヴォルヴァ・リミテッド 複数パラメータースクリーニングおよび複数機能性小分子産生細胞への進化方法
USRE47770E1 (en) 2002-07-18 2019-12-17 Merus N.V. Recombinant production of mixtures of antibodies
CA2872136C (en) 2002-07-18 2017-06-20 Merus B.V. Recombinant production of mixtures of antibodies
ATE527366T1 (de) * 2002-08-01 2011-10-15 Evolva Ltd Verfahren zur mischung von vielen heterologen genen
EP1639009B1 (en) 2003-05-30 2013-02-27 Merus B.V. Fab library for the preparation of a mixture of antibodies
US20100069614A1 (en) 2008-06-27 2010-03-18 Merus B.V. Antibody producing non-human mammals
JP5912211B2 (ja) * 2004-01-20 2016-04-27 メルス ビー．ヴィー． 結合タンパク質の混合物
CA2602183C (en) 2005-03-18 2014-05-06 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
WO2008042338A2 (en) 2006-09-28 2008-04-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
EP2370451B1 (en) 2008-12-02 2016-11-16 Wave Life Sciences Japan, Inc. Method for the synthesis of phosphorus atom modified nucleic acids
KR101153866B1 (ko) 2009-05-21 2012-06-27 대한민국 형질전환용 운반체, 이의 제조방법, 이를 이용한 형질전환방법 및 형질전환체
BR112012000828A8 (pt) 2009-07-06 2017-10-10 Ontorii Inc Novas pró-drogas de ácido nucleico e métodos de uso das mesmas
WO2011146833A1 (en) 2010-05-20 2011-11-24 Evolva Inc. Method of producing isoprenoid compounds in yeast
SG186127A1 (en) 2010-06-02 2013-01-30 Evolva Nutrition Inc Recombinant production of steviol glycosides
US10428019B2 (en) 2010-09-24 2019-10-01 Wave Life Sciences Ltd. Chiral auxiliaries
BR112014001244A2 (pt) 2011-07-19 2017-02-21 Wave Life Sciences Pte Ltd métodos para a síntese de ácidos nucléicos funcionalizados
EP3792350A1 (en) 2011-08-08 2021-03-17 Evolva SA Recombinant production of steviol glycosides
KR102269650B1 (ko) 2012-04-20 2021-06-28 메뤼스 엔.페. Ig-유사 분자의 제조방법 및 제조수단
WO2013191950A2 (en) 2012-06-22 2013-12-27 Monsanto Technology Llc Unique modular vector design
JP6246121B2 (ja) 2012-07-13 2017-12-13 株式会社新日本科学 キラル核酸アジュバント
JP6268157B2 (ja) 2012-07-13 2018-01-24 株式会社Ｗａｖｅ Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ Ｊａｐａｎ 不斉補助基
KR20240148947A (ko) 2012-07-13 2024-10-11 웨이브 라이프 사이언시스 리미티드 키랄 제어
WO2014066374A1 (en) * 2012-10-22 2014-05-01 Refactored Materials, Inc. Cellular reprogramming for product optimization
MY190346A (en) 2013-02-06 2022-04-15 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
MY184253A (en) 2013-02-11 2021-03-29 Evolva Sa Efficient production of steviol glycosides in recombinant hosts
JP6556122B2 (ja) 2013-09-17 2019-08-07 ボルト スレッズ インコーポレイテッド 改良シルク繊維を合成するための方法および組成物
US9580758B2 (en) 2013-11-12 2017-02-28 Luc Montagnier System and method for the detection and treatment of infection by a microbial agent associated with HIV infection
EP3095459A4 (en) 2014-01-15 2017-08-23 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having antitumor effect and antitumor agent
EP3095461A4 (en) 2014-01-15 2017-08-23 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having immunity induction activity, and immunity induction activator
WO2015108046A1 (ja) 2014-01-15 2015-07-23 株式会社新日本科学 抗アレルギー作用を有するキラル核酸アジュバンド及び抗アレルギー剤
CN106068325B (zh) 2014-01-16 2021-07-09 波涛生命科学有限公司 手性设计
KR20170041209A (ko) 2014-08-11 2017-04-14 에볼바 에스아 재조합 숙주에서 스테비올 글리코시드의 생성
EP4592304A3 (en) 2014-09-09 2025-11-05 Danstar Ferment Ag Production of steviol glycosides in recombinant hosts
BR112017016338A2 (pt) 2015-01-30 2018-03-27 Evolva Sa produção de glicosídeos de esteviol em hospedeiros recombinantes
US10604743B2 (en) 2015-03-16 2020-03-31 Dsm Ip Assets B.V. UDP-glycosyltransferases
US10837041B2 (en) 2015-08-07 2020-11-17 Evolva Sa Production of steviol glycosides in recombinant hosts
CN109195457A (zh) 2016-04-13 2019-01-11 埃沃尔瓦公司 在重组宿主中产生甜菊醇糖苷
EP3458599A1 (en) 2016-05-16 2019-03-27 Evolva SA Production of steviol glycosides in recombinant hosts
WO2018053204A1 (en) 2016-09-14 2018-03-22 Bolt Threads, Inc. Long uniform recombinant protein fibers
WO2018083338A1 (en) 2016-11-07 2018-05-11 Evolva Sa Production of steviol glycosides in recombinant hosts
JP2025170455A (ja) * 2022-10-05 2025-11-19 株式会社Logomix 細胞ライブラリおよびその製造方法
JP7212982B1 (ja) 2022-10-05 2023-01-26 株式会社Logomix 細胞ライブラリおよびその製造方法
US20240209383A1 (en) * 2022-12-21 2024-06-27 Clara Foods Co. Systems and methods for high yielding recombinant microorganisms and uses thereof

Family Cites Families (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1341302C (en) 1983-02-22 2001-10-09 Rae Lyn Burke Yeast expression systems with vectors having gapdh or pyk promoters and synthesis of foreign protein
ATE116442T1 (de) 1988-04-22 1995-01-15 Massachusetts Inst Technology Verfahren zum formen und zum gebrauch von mikrotröpchen.
DE69031710T2 (de) 1989-07-07 1998-03-05 Unilever N.V., Rotterdam Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopieintegrierung eines expressionsvektors transformierten pilzes
US5316922A (en) * 1992-04-15 1994-05-31 The United States Of America As Represented By Department Of Health And Human Services Method for indentifying and expressing proteins that recognize and adhere to specific probes
US5482845A (en) 1993-09-24 1996-01-09 The Trustees Of Columbia University In The City Of New York Method for construction of normalized cDNA libraries
CA2174354C (en) 1993-10-27 2011-03-29 Paul B. Fisher Compositions of melanoma differentiation associated gene-7 (mda-7) in cancer diagnosis and treatment
US6268140B1 (en) * 1994-02-03 2001-07-31 Neugenesis Combinatorial metabolic libraries
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5837458A (en) * 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6309883B1 (en) * 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
EP0822990A4 (en) * 1995-04-24 2002-07-03 Chromaxome Corp METHODS OF GENERATING AND SCREENING NEW METABOLIC PATHWAYS
US6030779A (en) * 1995-07-18 2000-02-29 Diversa Corporation Screening for novel bioactivities
US6004788A (en) * 1995-07-18 1999-12-21 Diversa Corporation Enzyme kits and libraries
US6057103A (en) * 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
US5958672A (en) * 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US5939250A (en) * 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US20010041333A1 (en) * 1997-06-16 2001-11-15 Short Jay M. High throughput screening for a bioactivity or biomolecule
US20030215798A1 (en) * 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
GB9603803D0 (en) * 1996-02-22 1996-04-24 Zeneca Ltd Production of proteins, plasmids coding therefor and organisms containing such plasmids
US6025155A (en) * 1996-04-10 2000-02-15 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US6242211B1 (en) 1996-04-24 2001-06-05 Terragen Discovery, Inc. Methods for generating and screening novel metabolic pathways
EP0922109A1 (en) 1996-05-21 1999-06-16 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
US5763239A (en) * 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
US6294325B1 (en) * 1996-07-05 2001-09-25 The Mount Sinai School Of Medicine Of The City University Of New York Cloning and expression of thermostable multi genes and proteins and uses thereof
JP2000515011A (ja) 1996-07-09 2000-11-14 アメリカ合衆国 形質転換関連組換えクローニング
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
JP4062366B2 (ja) 1997-01-17 2008-03-19 マキシジェン，インコーポレイテッド 再帰的配列組換えによる全細胞および生物の進化
JP3683591B2 (ja) 1997-02-27 2005-08-17 セロミックス インコーポレイテッド 細胞に基づくスクリーニングシステム
CA2284066A1 (en) 1997-03-18 1998-09-24 Chromaxome Corporation Methods for screening compounds using encapsulated cells
AU734761B2 (en) 1997-05-27 2001-06-21 Hanil Synthetic Fiber Co., Ltd. Process for preparing recombinant proteins using highly efficient expression vector from saccharomyces cerevisiae
GB9727512D0 (en) 1997-12-31 1998-02-25 Adprotech Plc Fuzzy genes and their application in molecular adjuvants
EP1060269A4 (en) 1998-03-04 2004-10-27 Diversa Corp PRODUCTION AND USE OF NORMALIZED DNA LIBRARIES
AU5217599A (en) 1998-07-27 2000-02-21 Genotypes, Inc. Automatic eukaryotic artificial chromosome vector
EP1102864B1 (en) 1998-08-07 2006-05-24 Cellay LLC Gel microdrops in genetic analysis
EP1109889A1 (en) 1998-08-31 2001-06-27 Maxygen, Inc. Transformation, selection, and screening of sequence-shuffled polynucleotides for development and optimization of plant phenotypes
EP1114320A2 (en) 1998-09-18 2001-07-11 Cellomics, Inc. A system for cell-based screening
JP2002537836A (ja) 1999-03-09 2002-11-12 ディベルサ コーポレーション 定方向進化におけるエンドセレクション
AU4039400A (en) 1999-03-26 2000-10-16 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
GB9908814D0 (en) * 1999-04-16 1999-06-09 Celltech Therapeutics Ltd Process
AU7646000A (en) 1999-11-05 2001-05-14 Novozymes A/S Methods to screen microorganisms or gene libraries for products secreted from a cell
WO2001073000A2 (en) * 2000-03-24 2001-10-04 Maxygen, Inc. Methods for modulating cellular and organismal phenotypes
EP1364005B1 (en) * 2001-01-25 2008-09-17 Evolva Ltd. A method for evolving a cell having desired phenotype and evolved cells
US8008459B2 (en) * 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
DE60239386D1 (de) 2001-01-25 2011-04-21 Evolva Ltd Zellbibliothek
CA2474161C (en) 2001-01-25 2012-03-27 Evolva Ltd. Concatemers of differentially expressed multiple genes
WO2002059330A2 (en) 2001-01-25 2002-08-01 Evolva Biotech A/S Artificial chromosomes comprising concatemers of expressible nucleotide sequences
JP4472345B2 (ja) 2002-01-25 2010-06-02 エヴォルヴァ・リミテッド 複数パラメータースクリーニングおよび複数機能性小分子産生細胞への進化方法
ATE386810T1 (de) 2002-06-14 2008-03-15 Dyax Corp Rekombination von nukleinsäurebibliotheksmitgliedern
US20030235823A1 (en) * 2002-06-24 2003-12-25 The University Of Alabama Nucleotide sequences that code for torsin genes, torsin proteins, and methods of using the same to treat protein-aggregation
ATE527366T1 (de) * 2002-08-01 2011-10-15 Evolva Ltd Verfahren zur mischung von vielen heterologen genen

Also Published As

Publication number Publication date
AU2002227883A1 (en) 2002-08-06
WO2002059297A2 (en) 2002-08-01
EP1356037A2 (en) 2003-10-29
JP2010043108A (ja) 2010-02-25
WO2002059297A8 (en) 2004-04-15
CA2474172C (en) 2012-07-10
US20040241672A1 (en) 2004-12-02
JP4965625B2 (ja) 2012-07-04
US20120094870A1 (en) 2012-04-19
US7838287B2 (en) 2010-11-23
ATE501251T1 (de) 2011-03-15
CA2474172A1 (en) 2002-08-01
EP1356037B1 (en) 2011-03-09
JP2004525624A (ja) 2004-08-26
DE60239386D1 (de) 2011-04-21
WO2002059297A3 (en) 2003-04-10

Similar Documents

Publication Publication Date Title
DK1356037T3 (da) Bibliotek med en samling af celler
Bembenek et al. Ligand efficiency and fragment-based drug discovery
Vazquez-Anderson et al. Regulatory RNAs: charming gene management styles for synthetic biology applications
Friedman et al. Epigenomics and the structure of the living genome
Tsai et al. Long intergenic noncoding RNAs: new links in cancer progression
Nasiri et al. Targeting a c-MYC G-quadruplex DNA with a fragment library
Luo et al. Guidance of circular RNAs to proteins’ behavior as binding partners
Gawron et al. The proteome under translational control
Olson The nucleolus
DK1390492T3 (da) Dirigeret evolution af biomolekyler ved anvendelse af mikrostrukturer
DE602005014081D1 (de) Mehrfacher ungenauer mustervergleich
Kent et al. Broad H3K4me3 domains: Maintaining cellular identity and their implication in super‐enhancer hijacking
Bortnick et al. Cellular and chromatin dynamics of antibody‐secreting plasma cells
Narberhaus et al. Regulatory RNAs in prokaryotes: here, there and everywhere
Bangi A drosophila based cancer drug discovery framework
Wucherpfennig et al. Genetic screens to study the immune system in cancer
WO2004094610A3 (en) Wnt as a factor for cardiac myogenesis
Wassermann et al. Comprehensive analysis of single‐and multi‐target activity cliffs formed by currently available bioactive compounds
Bai et al. lncRNA expression reveals the potential regulatory roles in hepatocyte proliferation during rat liver regeneration
AU2003247270A1 (en) Methods of mixing large numbers of heterologous genes
Sayut et al. Engineering and applications of genetic circuits
Bi et al. Bifurcation analysis and potential landscapes of the p53-Mdm2 module regulated by the co-activator programmed cell death 5
IL180531A0 (en) Novel sequence for improving expression of nucleic acid
DE69931759D1 (de) Methode zur erstellung von genexpressionsprofilen
Zhalnina et al. Need for laboratory ecosystems to unravel the structures and functions of soil microbial communities mediated by chemistry. mBio 9: e01175-18