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DK1095056T3 - Höjtryksrefoldning af proteinaggregater og inklusionslegemer - Google Patents

Höjtryksrefoldning af proteinaggregater og inklusionslegemer

Info

Publication number
DK1095056T3
DK1095056T3 DK99933769T DK99933769T DK1095056T3 DK 1095056 T3 DK1095056 T3 DK 1095056T3 DK 99933769 T DK99933769 T DK 99933769T DK 99933769 T DK99933769 T DK 99933769T DK 1095056 T3 DK1095056 T3 DK 1095056T3
Authority
DK
Denmark
Prior art keywords
kbar
refolding
solution
inclusion bodies
aggregates
Prior art date
Application number
DK99933769T
Other languages
English (en)
Inventor
John F Carpenter
Theodore W Randolph
John Richard St
Original Assignee
Barofold Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22232166&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK1095056(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Barofold Inc filed Critical Barofold Inc
Application granted granted Critical
Publication of DK1095056T3 publication Critical patent/DK1095056T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Endocrinology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
DK99933769T 1998-07-09 1999-07-09 Höjtryksrefoldning af proteinaggregater og inklusionslegemer DK1095056T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9220898P 1998-07-09 1998-07-09
PCT/US1999/015419 WO2000002901A1 (en) 1998-07-09 1999-07-09 High pressure refolding of protein aggregates and inclusion bodies

Publications (1)

Publication Number Publication Date
DK1095056T3 true DK1095056T3 (da) 2007-04-23

Family

ID=22232166

Family Applications (1)

Application Number Title Priority Date Filing Date
DK99933769T DK1095056T3 (da) 1998-07-09 1999-07-09 Höjtryksrefoldning af proteinaggregater og inklusionslegemer

Country Status (7)

Country Link
US (5) US6489450B2 (da)
EP (1) EP1095056B1 (da)
AT (1) ATE349460T1 (da)
AU (1) AU4975699A (da)
DE (1) DE69934585T2 (da)
DK (1) DK1095056T3 (da)
WO (1) WO2000002901A1 (da)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4975699A (en) * 1998-07-09 2000-02-01 University Technology Corporation High pressure refolding of protein aggregates and inclusion bodies
EP1204855A1 (en) * 1999-07-16 2002-05-15 Human Genome Sciences, Inc. Real-time, in situ biomanufacturing process monitoring and control in response to ir spectroscopy
US7615617B2 (en) * 1999-10-25 2009-11-10 University Of Delaware Use of hydrostatic pressure to inhibit and reverse protein aggregation and facilitate protein refolding
ATE390436T1 (de) * 2000-10-31 2008-04-15 Barofold Inc Verbesserter zerfall und rückfaltung von proteinen mittels hohen drucks
US8815557B2 (en) 2005-07-08 2014-08-26 University Of Chicago Compositions and methods for refolding of denatured proteins
US20070141341A1 (en) * 2005-12-15 2007-06-21 Dewald Aaron Coating containing denatured aloe vera, for covering either chlorine-generating compositions or other hazardous chemicals and method for making
GB0605684D0 (en) * 2006-03-21 2006-05-03 Sicor Biotech Uab Method For Purifying Granulocyte-Colony Stimulating Factor
WO2008033556A2 (en) * 2006-09-15 2008-03-20 Barofold, Inc. High pressure treatment of proteins for reduced immunogenicity
WO2008033555A2 (en) * 2006-09-15 2008-03-20 Barofold, Inc. High-pressure refolding of difficult-to-fold proteins
US7829681B2 (en) * 2007-04-05 2010-11-09 Barofold Inc. High-pressure inclusion body solubilization and protease clipping of recombinant fusion proteins
US20080249286A1 (en) * 2007-04-05 2008-10-09 Seefeldt Matthew B High-pressure refolding of proteins in the presence of binding partners
EP2155351B1 (en) * 2007-06-04 2021-02-17 Pressure Biosciences, Inc. Pressure-enhanced extraction and partitioning of molecules
WO2009035617A1 (en) * 2007-09-10 2009-03-19 Barofold, Inc. High-pressure refolding of monoclonal antibody aggregates
EP2207890A4 (en) * 2007-10-05 2010-12-15 Barofold Inc HIGH PRESSURE PROCESSING OF AGGREGATED INTERFERONS
US20090286301A1 (en) * 2008-05-07 2009-11-19 Pressure Biosciences Inc. Extraction of biomolecular complexes assisted by alternating hydrostatic pressure
US20100112660A1 (en) * 2008-05-30 2010-05-06 Barofold, Inc. Method for Derivatization of Proteins Using Hydrostatic Pressure
ES2749977T3 (es) 2010-07-19 2020-03-24 Pressure Biosciences Inc Composiciones de proteínas terapéuticas que tienen inmunogenia reducida y/o eficacia mejorada
WO2012024523A2 (en) 2010-08-18 2012-02-23 Ting Edmund Y Flow-through high hydrostatic pressure microfluidic sample preparation device and related methods therefor
EP2632594A1 (en) 2010-10-28 2013-09-04 Edmund Y. Ting System and method for microplate pressurization
EP2785196B1 (en) 2011-12-01 2020-08-05 Pressure BioSciences, Inc. Methods and systems for protein refolding
HUP1200172A2 (en) 2012-03-19 2013-10-28 Richter Gedeon Nyrt Methods for refolding g-csf from inclusion bodies
HUP1200171A1 (hu) 2012-03-19 2013-09-30 Richter Gedeon Nyrt Módszerek polipeptidek elõállítására
NZ706288A (en) 2012-08-30 2016-06-24 Merial Inc Expression of chimeric ksac protein and method of producing soluble proteins by high pressure
ZA201501696B (en) 2012-08-30 2016-01-27 Merial Ltd Hyperbaric device and methods for producing inactivated vaccines and for refolding/solubilizing recombinant proteins
WO2021050009A2 (en) * 2019-09-13 2021-03-18 National Science And Technology Development Agency Composition of renaturation buffer solution for dimeric proteins and method of renaturation dimeric proteins using the composition thereof
FI4263568T3 (fi) 2020-12-18 2025-09-25 Richter Gedeon Nyrt Menetelmät uudelleen laskostuneen fc-peptidifuusioproteiinin puhdistamiseksi
CN113683676A (zh) * 2021-08-27 2021-11-23 恒敬合创生物医药(上海)有限公司 一种环保的可逆的蛋白变性工艺

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4234620A (en) 1978-12-26 1980-11-18 A. E. Staley Manufacturing Company Water-soluble vegetable protein aggregates
US4652630A (en) 1985-02-22 1987-03-24 Monsanto Company Method of somatotropin naturation
US4659568A (en) 1985-02-27 1987-04-21 American Cyanamid Company Process for solubilization, purification and characterization of protein from insoluble protein aggregates or complexes and compositions of matter therefrom
US4766224A (en) * 1985-08-19 1988-08-23 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
US4677196A (en) 1985-09-06 1987-06-30 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
US5453363A (en) 1985-10-23 1995-09-26 Boehringer Mannheim Gmbh Process for the activation of t-PA or Ing after genetic expression in prokaryotes
US4929700A (en) * 1987-04-16 1990-05-29 Cetus Corporation Production of purified, biologically active, bacterially produced recombinant human CSF-1
US5162507A (en) 1987-05-11 1992-11-10 Cetus Corporation Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
US4985544A (en) 1987-08-04 1991-01-15 Kyowa Hakko Kogyo Co., Ltd. Process for renaturing fish growth hormone
US4923967A (en) 1988-09-26 1990-05-08 Eli Lilly And Company Purification and refolding of recombinant proteins
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
US5064943A (en) 1988-12-16 1991-11-12 American Cyanamid Company Method for solubilization and naturation of somatotropin
GB8927008D0 (en) * 1989-11-29 1990-01-17 Ciba Geigy Novel process for the production of unfused protein in e.coli
US5109117A (en) 1990-06-26 1992-04-28 Monsanto Company Method of somatotropin naturation using urea and a soluble organic alcohol
US5023323A (en) 1990-09-12 1991-06-11 Monsanto Company Method of somatotropin naturation
US5288931A (en) 1991-12-06 1994-02-22 Genentech, Inc. Method for refolding insoluble, misfolded insulin-like growth factor-I into an active conformation
US5288462A (en) 1992-05-18 1994-02-22 Stephen D. Carter Sterilization apparatus and method
WO1994000406A1 (en) * 1992-06-29 1994-01-06 United Technologies Corporation Beneficial use of energy-containing wastes
US5605691A (en) 1992-09-17 1997-02-25 Ophidian Pharmaceuticals, Inc. Immunologically active proteins from inclusion bodies
US5714371A (en) 1995-05-12 1998-02-03 Schering Corporation Method for refolding insoluble aggregates of hepatitis C virus protease
US5728804A (en) 1995-06-02 1998-03-17 Research Corporation Technologies, Inc. Use of cyclodextrins for protein renaturation
US5800811A (en) * 1995-06-06 1998-09-01 Hall; Frederick L. Artificial skin prepared from coclagen matrix containing transforming growth factor-β having a collagen binding site
DE69707140T2 (de) 1996-07-02 2002-06-20 Bbi Bioseq, Inc. Druck-ermittelte bindung von biomolekularen komplexen
AU4975699A (en) 1998-07-09 2000-02-01 University Technology Corporation High pressure refolding of protein aggregates and inclusion bodies
US7615617B2 (en) * 1999-10-25 2009-11-10 University Of Delaware Use of hydrostatic pressure to inhibit and reverse protein aggregation and facilitate protein refolding
ATE390436T1 (de) 2000-10-31 2008-04-15 Barofold Inc Verbesserter zerfall und rückfaltung von proteinen mittels hohen drucks

Also Published As

Publication number Publication date
US20060188970A1 (en) 2006-08-24
US20020137157A1 (en) 2002-09-26
AU4975699A (en) 2000-02-01
US20110046357A1 (en) 2011-02-24
WO2000002901A1 (en) 2000-01-20
US8710197B2 (en) 2014-04-29
US7064192B2 (en) 2006-06-20
DE69934585T2 (de) 2007-10-25
EP1095056A1 (en) 2001-05-02
US20100255536A1 (en) 2010-10-07
EP1095056B1 (en) 2006-12-27
DE69934585D1 (de) 2007-02-08
US7767795B2 (en) 2010-08-03
US20030083475A1 (en) 2003-05-01
US6489450B2 (en) 2002-12-03
ATE349460T1 (de) 2007-01-15

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