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DK1058738T3 - Fremgangsmåde til screening, baseret på fluorescens-polarisering - Google Patents

Fremgangsmåde til screening, baseret på fluorescens-polarisering

Info

Publication number
DK1058738T3
DK1058738T3 DK99906092T DK99906092T DK1058738T3 DK 1058738 T3 DK1058738 T3 DK 1058738T3 DK 99906092 T DK99906092 T DK 99906092T DK 99906092 T DK99906092 T DK 99906092T DK 1058738 T3 DK1058738 T3 DK 1058738T3
Authority
DK
Denmark
Prior art keywords
fluorescence polarization
screening based
transferase
screening
determination
Prior art date
Application number
DK99906092T
Other languages
English (en)
Inventor
Lars Kongsbak
Steffen Ernst
Kristian Skovgaard Joergensen
Jesper Valbjoern
Christel Thea Joergensen
Tommy Lykke Husum
Soeren Moeller
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK1058738T3 publication Critical patent/DK1058738T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1086Preparation or screening of expression libraries, e.g. reporter assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/40Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6445Measuring fluorescence polarisation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/91Transferases (2.)
    • G01N2333/91045Acyltransferases (2.3)
    • G01N2333/91074Aminoacyltransferases (general) (2.3.2)
    • G01N2333/9108Aminoacyltransferases (general) (2.3.2) with definite EC number (2.3.2.-)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/914Hydrolases (3)
    • G01N2333/916Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G01N2333/918Carboxylic ester hydrolases (3.1.1)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/914Hydrolases (3)
    • G01N2333/924Hydrolases (3) acting on glycosyl compounds (3.2)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Materials By The Use Of Optical Means Adapted For Particular Applications (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
DK99906092T 1998-03-06 1999-03-05 Fremgangsmåde til screening, baseret på fluorescens-polarisering DK1058738T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK30898 1998-03-06
PCT/DK1999/000112 WO1999045143A2 (en) 1998-03-06 1999-03-05 A fluorescence polarization screening method

Publications (1)

Publication Number Publication Date
DK1058738T3 true DK1058738T3 (da) 2002-03-11

Family

ID=8092089

Family Applications (1)

Application Number Title Priority Date Filing Date
DK99906092T DK1058738T3 (da) 1998-03-06 1999-03-05 Fremgangsmåde til screening, baseret på fluorescens-polarisering

Country Status (11)

Country Link
EP (2) EP1058738B1 (da)
JP (1) JP2002505114A (da)
CN (2) CN1560609A (da)
AT (1) ATE208828T1 (da)
AU (1) AU2612299A (da)
CA (1) CA2322488A1 (da)
DE (1) DE69900465T2 (da)
DK (1) DK1058738T3 (da)
ES (1) ES2168848T3 (da)
PT (1) PT1058738E (da)
WO (1) WO1999045143A2 (da)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69824895T2 (de) * 1998-04-15 2005-06-30 Hidaka, Hiroyoshi, Nagoya Verfahren zur bestimmung des gens eines zielproteins mit hilfe eines medikamentes
CA2385323A1 (en) * 1999-10-01 2001-04-12 Novozymes A/S Cellulose films for screening
US6426189B1 (en) 1999-10-01 2002-07-30 Novozymes A/S Cellulose films for screening
AU7646100A (en) * 1999-11-05 2001-05-14 Novozymes A/S A high throughput screening (hts) method
US6723537B2 (en) 2001-05-18 2004-04-20 Rigel Pharmaceuticals, Incorporated Directed evolution of protein in mammalian cells
ES2337699T3 (es) * 2004-03-18 2010-04-28 Transtech Pharma, Inc. Ensayo por polarizacion de fluorescencia.
FR2897164B1 (fr) 2006-02-09 2008-03-14 Commissariat Energie Atomique Realisation de cavites pouvant etre remplies par un materiau fluidique dans un compose microtechnologique optique
FR2929715B1 (fr) 2008-04-04 2010-09-10 Commissariat Energie Atomique Procede de realisation de cavites microniques ou submicroniques
FR2933205B1 (fr) 2008-06-27 2010-08-13 Commissariat Energie Atomique Procede ameliore de realisation d'enceintes remplies de liquide et fermees par une membrane
FR2938834B1 (fr) * 2008-11-27 2011-03-04 Commissariat Energie Atomique Procede de realisation d'une matrice de retention et comprenant un liquide fonctionnel
FR2963112B1 (fr) 2010-07-21 2013-02-15 Commissariat Energie Atomique Microstructure a parois a propriete optique determinee et procede de realisation de microstructures
WO2014067933A1 (en) 2012-10-31 2014-05-08 C-Lecta Gmbh Bioactive carrier preparation for enhanced safety in care products and food
KR101494881B1 (ko) 2013-05-06 2015-02-24 한국과학기술연구원 설퍼 옥시도리덕타아제 결합 형광 화합물을 이용한 바실러스 서브틸리스 검출 방법
CN105866316B (zh) * 2016-06-24 2018-03-30 曲阜师范大学 一种同时检测食品中氨基酸和生物胺的分析方法
CN110954517A (zh) * 2019-12-11 2020-04-03 西北大学 还原性糖的一步荧光衍生方法及其应用
CN113514445B (zh) * 2021-08-05 2023-12-26 荆州市第一人民医院(荆州市肿瘤医院、长江大学附属第一医院) 一种基于荧光偏振的去泛素化酶活性检测方法
CN114166807B (zh) * 2021-11-22 2023-12-12 中国农业大学 用于检测多粘菌素的荧光偏振免疫分析方法
WO2025168976A1 (en) * 2024-02-10 2025-08-14 Biuro Techniczno-Prawne Adam Turczyński Method for detecting pectinolytic pathogens of crops and ornamental plants, microbial medium for use in this method and test kit implementing this method
WO2025168975A1 (en) * 2024-02-10 2025-08-14 Biuro Techniczno-Prawne Adam Turczyński Method of manufacturing a probe for determining the activity of pectinolytic enzymes and method of determining the activity of pectinolytic enzymes using said probe

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0194472B1 (en) * 1985-02-25 1992-07-22 Abbott Laboratories Fluorescence polarization assay for macromolecular hydrolases.
MX9206979A (es) * 1991-12-04 1993-07-01 Novo Nordisk As Metodo de clonacion de proteinas en levaduras
JPH0723800A (ja) * 1993-07-08 1995-01-27 Tanabe Seiyaku Co Ltd 核酸の検出方法
GB9519393D0 (en) * 1995-09-22 1995-11-22 Univ Edinburgh Novel compounds and assay method

Also Published As

Publication number Publication date
EP1058738A2 (en) 2000-12-13
DE69900465D1 (de) 2001-12-20
EP1156122A3 (en) 2005-02-09
CA2322488A1 (en) 1999-09-10
DE69900465T2 (de) 2002-09-05
EP1058738B1 (en) 2001-11-14
JP2002505114A (ja) 2002-02-19
ATE208828T1 (de) 2001-11-15
WO1999045143A3 (en) 1999-10-21
ES2168848T3 (es) 2002-06-16
AU2612299A (en) 1999-09-20
WO1999045143A2 (en) 1999-09-10
CN1560609A (zh) 2005-01-05
CN1297487A (zh) 2001-05-30
PT1058738E (pt) 2002-05-31
EP1156122A2 (en) 2001-11-21
CN1167810C (zh) 2004-09-22

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