[go: up one dir, main page]

DE69932944D1 - Kolonne und mit dieser durchgeführtes chromatographisches verfahren - Google Patents

Kolonne und mit dieser durchgeführtes chromatographisches verfahren

Info

Publication number
DE69932944D1
DE69932944D1 DE69932944T DE69932944T DE69932944D1 DE 69932944 D1 DE69932944 D1 DE 69932944D1 DE 69932944 T DE69932944 T DE 69932944T DE 69932944 T DE69932944 T DE 69932944T DE 69932944 D1 DE69932944 D1 DE 69932944D1
Authority
DE
Germany
Prior art keywords
ion exchange
zwitterionic
colonne
carried out
chromatographic method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69932944T
Other languages
English (en)
Other versions
DE69932944T2 (de
Inventor
Knut Irgum
Camilla Viklund
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Patent GmbH
Original Assignee
Sequant AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sequant AB filed Critical Sequant AB
Publication of DE69932944D1 publication Critical patent/DE69932944D1/de
Application granted granted Critical
Publication of DE69932944T2 publication Critical patent/DE69932944T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/26—Cation exchangers for chromatographic processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/364—Amphoteric or zwitterionic ion-exchanger
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
    • B01J20/28078—Pore diameter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/289—Phases chemically bonded to a substrate, e.g. to silica or to polymers bonded via a spacer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/3092—Packing of a container, e.g. packing a cartridge or column
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/321—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
    • B01J20/3219—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond involving a particular spacer or linking group, e.g. for attaching an active group
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3251—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising at least two different types of heteroatoms selected from nitrogen, oxygen or sulphur
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3255—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising a cyclic structure containing at least one of the heteroatoms nitrogen, oxygen or sulfur, e.g. heterocyclic or heteroaromatic structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/3278—Polymers being grafted on the carrier
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3285—Coating or impregnation layers comprising different type of functional groups or interactions, e.g. different ligands in various parts of the sorbent, mixed mode, dual zone, bimodal, multimodal, ionic or hydrophobic, cationic or anionic, hydrophilic or hydrophobic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3291—Characterised by the shape of the carrier, the coating or the obtained coated product
    • B01J20/3293—Coatings on a core, the core being particle or fiber shaped, e.g. encapsulated particles, coated fibers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J43/00—Amphoteric ion-exchange, i.e. using ion-exchangers having cationic and anionic groups; Use of material as amphoteric ion-exchangers; Treatment of material for improving their amphoteric ion-exchange properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/58—Use in a single column
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/80—Aspects related to sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J2220/82—Shaped bodies, e.g. monoliths, plugs, tubes, continuous beds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/52—Physical parameters
    • G01N2030/524—Physical parameters structural properties
    • G01N2030/528—Monolithic sorbent material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
DE69932944T 1998-11-09 1999-11-09 Chromatographisches verfahren und für das verfahren geeignetes säulenmaterial Expired - Lifetime DE69932944T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE9803838A SE9803838D0 (sv) 1998-11-09 1998-11-09 A chromatography method and a column material useful in said method
SE9803838 1998-11-09
PCT/SE1999/002032 WO2000027496A1 (en) 1998-11-09 1999-11-09 A chromatography method and a column material useful in said method

Publications (2)

Publication Number Publication Date
DE69932944D1 true DE69932944D1 (de) 2006-10-05
DE69932944T2 DE69932944T2 (de) 2007-05-10

Family

ID=20413235

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69932944T Expired - Lifetime DE69932944T2 (de) 1998-11-09 1999-11-09 Chromatographisches verfahren und für das verfahren geeignetes säulenmaterial

Country Status (9)

Country Link
US (1) US6884345B1 (de)
EP (1) EP1137466B1 (de)
JP (1) JP4440474B2 (de)
AT (1) ATE337058T1 (de)
AU (1) AU759683B2 (de)
CA (1) CA2349948C (de)
DE (1) DE69932944T2 (de)
SE (1) SE9803838D0 (de)
WO (1) WO2000027496A1 (de)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10013990C2 (de) * 2000-03-22 2003-12-04 Invitek Biotechnik & Biodesign Polyfunktionelles Trägermaterial für die komplexe Nukleinsäureanalytik
US7238426B2 (en) * 2003-08-13 2007-07-03 Sequant Ab Column packing material
US7250388B2 (en) * 2003-11-07 2007-07-31 Hewlett-Packard Development Company, L.P. Medium for chromatographic separations
JP3922648B2 (ja) * 2004-05-24 2007-05-30 株式会社資生堂 アフィニティー粒子及びアフィニティー分離方法
CA2578202C (en) * 2004-08-30 2013-12-17 Toray Industries, Inc. Fractionation apparatus
US20100300971A1 (en) * 2005-05-18 2010-12-02 Sequant Ab Zwitterionic stationary phase as well as method for using and producing said phase
SE529259C2 (sv) * 2005-08-31 2007-06-12 Ge Healthcare Bio Sciences Ab Tillverkning av kromarografimatriser, en kromarografimatris, en vätskekromatografikolonn, prcess för att isolera målföreningar och användning av en kromarografimatris för vätskekromatografi
SE0502485L (sv) * 2005-11-09 2007-05-10 Peter Viberg Partiklar
EP1962097A1 (de) * 2007-02-23 2008-08-27 SeQuant AB Massenspektrometrischer quantitativer Nachweis von Methylmalonsäure und Bernsteinsäure unter Verwendung von HILIC auf einer zwitterionischen stationären Phase
JP2009069050A (ja) 2007-09-14 2009-04-02 Nec Corp 化合物のカラム担体への固定化方法
US9051349B2 (en) 2007-11-21 2015-06-09 Alba Therapeutics Corporation Larazotide acetate compositions
EP2062909A1 (de) * 2007-11-21 2009-05-27 SOLVAY (Société Anonyme) Herstellung von Peptiden und Reinigungsverfahren
JP5689791B2 (ja) * 2008-05-13 2015-03-25 ウニベルジテート ウィーン エナンチオ選択的両性イオン性イオン交換材料
US20090294362A1 (en) * 2008-05-30 2009-12-03 Persson Jonas Par Stationary phase for hydrophilic interaction chromatography
WO2010027955A2 (en) 2008-09-02 2010-03-11 Natrix Separations Inc. Chromatography membranes, devices containing them, and methods of use thereof
DE102009006007B4 (de) * 2009-01-23 2015-03-12 Axagarius Gmbh & Co. Kg Verwendung von zwitterionischen Adsorbentien für die Flüssigchromatographie, Chromatographiesäule mit solchen Adsorbentien sowie deren Verwendung
US10159911B2 (en) 2009-08-04 2018-12-25 Waters Technologies Corporation High purity chromatographic materials comprising an ionizable modifier
EP2462188A4 (de) * 2009-08-04 2016-11-23 Waters Technologies Corp Hochreine chromatographiematerialien mit einem ionisierbaren modifikator
EP2499192B1 (de) * 2009-11-13 2020-07-15 Merck Millipore Ltd. Membranen für hydrophobe interaktionschromatographie
EP2521612B1 (de) * 2010-01-08 2017-05-17 University Of Tasmania Verwendung von porösen polymermonolithen
EP2571487B1 (de) 2010-05-21 2023-01-04 Siemens Healthcare Diagnostics Inc. Zwitterionische reagenzien
JP5812464B2 (ja) * 2010-07-01 2015-11-11 積水メディカル株式会社 標的核酸の分離検出方法、標的核酸分離検出用イオン交換クロマトグラフィー用充填剤、及び標的核酸分離検出用イオン交換クロマトグラフィー用カラム
KR101997543B1 (ko) * 2010-07-30 2019-07-09 이엠디 밀리포어 코포레이션 크로마토그래피 매질 및 방법
CA2814942C (en) 2010-10-27 2014-12-16 Toray Industries, Inc. Carrier for blood component adsorption and blood component adsorption column
JP5926484B2 (ja) 2010-11-18 2016-05-25 株式会社セルシード グリコサミノグリカンの新規な分析方法
AU2011343813B2 (en) 2010-12-15 2015-05-21 Takeda Pharmaceutical Company Limited Eluate collection using conductivity gradient
CN102614847B (zh) 2011-01-28 2013-12-18 浙江华谱新创科技有限公司 一种两性离子亲水色谱固定相及其制备方法
JP5734320B2 (ja) * 2011-02-10 2015-06-17 積水メディカル株式会社 イオン交換クロマトグラフィー用充填剤及び核酸鎖の分離検出方法
US20140056858A1 (en) 2011-02-28 2014-02-27 National University Corporation Hokkaido University Cell sheet having hyaluronic acid production ability and method for preparing the same
JP5807229B2 (ja) * 2011-04-12 2015-11-10 学校法人中部大学 マイコトキシンのスクリーニング法
CA2836460C (en) 2011-05-17 2021-09-21 Natrix Separations Inc. Methods of using a fluid treatment device
CA2836812C (en) 2011-07-12 2018-07-10 University Of Tasmania Use of porous polymer materials for storage of biological samples
CN103120932B (zh) * 2013-03-01 2014-12-17 华东理工大学 一种阳离子多糖涂覆型亲水色谱固定相的制备方法及应用
US10494538B2 (en) * 2013-05-30 2019-12-03 The University Of Akron Switchable antimicrobial and antifouling carboxybetaine-based hydrogels and elastomers with enhanced mechanical properties
CN105377873B (zh) 2013-07-12 2021-03-30 豪夫迈·罗氏有限公司 阐明离子交换层析输入优化
US9340562B1 (en) * 2014-10-29 2016-05-17 Thermo Electron Manufacturing Limited Chromatographic material and method for preparation thereof
JP6729041B2 (ja) * 2016-06-17 2020-07-22 日立化成株式会社 分離材及びカラム
JP7089540B2 (ja) * 2017-06-22 2022-06-22 アーチャー－ダニエルズ－ミッドランド カンパニー ジカルボン酸含有混合物の分離及び精製方法
CN107597079B (zh) * 2017-10-17 2020-06-16 暨南大学 一类基于两性离子交联剂的有机聚合物整体材料及制备和应用
CN109806916B (zh) * 2019-03-15 2021-12-21 中科森辉微球技术（苏州）有限公司 高性能阴离子交换介质及其制备方法
CN114618454A (zh) * 2020-12-11 2022-06-14 中国科学院大连化学物理研究所 一种极性包埋双链反相色谱固定相及其制备和应用
CN114190313B (zh) * 2021-11-25 2023-04-11 舒城县汇德水产科技有限公司 一种环保型高密度红螯螯虾的繁殖育苗方法
WO2025179026A1 (en) * 2024-02-21 2025-08-28 Life Technologies Corporation Multivalent cation-binding ligands and compositions comprising the same for ionic chelate affinity separations

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4577013A (en) * 1983-12-22 1986-03-18 Ciba-Geigy Corporation Ionically modified cellulose material, its preparation and its use
US6039876A (en) * 1992-07-24 2000-03-21 Yang; Yan-Bo Hydrophilic polystrene divinylbenzene based matrixes for chromatography
JP3351572B2 (ja) * 1992-10-05 2002-11-25 井上 聰一 イオンクロマトグラフィーによる分析体の分離・分析方法、イオンクロマトグラフィー用両荷電具備固定相及び多機能液体クロマトグラフィーによる分析体の分離・分析方法
AU7353298A (en) 1997-04-25 1998-11-24 Diamyd Therapeutics Ab Use of a zwitterionic detergent/surfactant for purifying glutamic acid decarboxylase (gad) and a composition containing gad
DE19721601A1 (de) * 1997-05-23 1998-11-26 Hoechst Ag Polybetain-stabilisierte, Palladium-haltige Nanopartikel, ein Verfahren zu ihrer Herstellung sowie daraus hergestellte Katalysatoren zur Gewinnung von Vinylacetat
DE19754304A1 (de) * 1997-12-08 1999-06-10 Hoechst Ag Polybetain-stabilisierte Platin-Nanopartikel, Verfahren zu ihrer Herstellung und Verwendung für Elektrokatalysatoren in Brennstoffzellen
US6238565B1 (en) * 1998-09-16 2001-05-29 Varian, Inc. Monolithic matrix for separating bio-organic molecules

Also Published As

Publication number Publication date
ATE337058T1 (de) 2006-09-15
EP1137466B1 (de) 2006-08-23
CA2349948A1 (en) 2000-05-18
AU1591900A (en) 2000-05-29
JP4440474B2 (ja) 2010-03-24
CA2349948C (en) 2008-10-14
SE9803838D0 (sv) 1998-11-09
JP2002529714A (ja) 2002-09-10
AU759683B2 (en) 2003-04-17
WO2000027496A1 (en) 2000-05-18
EP1137466A1 (de) 2001-10-04
US6884345B1 (en) 2005-04-26
DE69932944T2 (de) 2007-05-10

Similar Documents

Publication Publication Date Title
DE69932944D1 (de) Kolonne und mit dieser durchgeführtes chromatographisches verfahren
DK1015586T3 (da) TIE-receptortyrosinkinaseligandhomologer
ATE384742T1 (de) Verfahren zur reinigung von preproinsulin
ATE470150T1 (de) Methode zum schnellen identifizieren von kleinen organischen liganden
DE60140474D1 (de) Sammlung von proteinen mit sich wiederholenden sequenzen (repeat proteins), die repetitive sequenzmodule enthalten
WO2002085519A3 (en) High capacity methods for separation, purification, concentration, immobilization and synthesis of compounds and applications based thereupon
ATE310090T1 (de) Gereinigte coprinus laccasen und nukleinsäuren welche dafür kodieren
ATE425989T1 (de) Proteinreinigungsverfahren
EP1961826A3 (de) Verfahren zur direkten Sequenzierung von Nukleinsäuren
ES2196011T3 (es) Reactivos multifuncionales restringidos para modificacion de superficie.
WO2000044928A3 (en) Materials and methods for the purification of polyelectrolytes
DE69433690D1 (de) Chimere proteine, welche borrelia polypeptide beinhalten, verwendungen dafür
ATE393217T1 (de) Verfahren zur reinigung von faktor-ix
ATE307649T1 (de) Kontinuierliches verfahren zur trennung von stoffen nach molekülgrösse
DK1230392T3 (da) Sikkerhedssystem
EP0335654A3 (de) Riechstoff bindendes Protein aus Ratten
EP1347831A4 (de) Auf stimuli ansprechendes material zur affinitätschromatographie und trenn- und reinigungsverfahren dafür
DE69718054D1 (de) Verfahren zur aufreinigung von dns
ATE428728T1 (de) Verfahren zur reinigung von erythropoietin
ATE186781T1 (de) Verfahren zur herstellung von testelementen
DE68925588D1 (de) Verfahren zur aktivierung von polymeren trägern und damit hergestellte zusammensetzungen zur verwendung in der affinitätschromatographie
DK0501309T3 (da) Fyldmaterialer, der kombinerer protein med en bærer via et afstandstykke.
WO2005014779A3 (en) Cyclodextrin affinity purification
DE69811677D1 (de) Verfahren zur präparation von enantiomer-angereicherten, n-derivatisierten laktamen.
DK1208213T3 (da) Fremgangsmåde til fremstilling af proteiner i værtsceller under anvendelse af chaperoniner

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: MERCK PATENT GMBH, 64293 DARMSTADT, DE