DE69924873D1 - Cytochrom p450 monooxygenase und nadph-cytochrom p450-oxidoreduktase gene und proteine mit bezug zum omega-hydroxylase-komplex in candida tropicalis und dazu gehörigen verfahren - Google Patents
Cytochrom p450 monooxygenase und nadph-cytochrom p450-oxidoreduktase gene und proteine mit bezug zum omega-hydroxylase-komplex in candida tropicalis und dazu gehörigen verfahrenInfo
- Publication number
- DE69924873D1 DE69924873D1 DE69924873T DE69924873T DE69924873D1 DE 69924873 D1 DE69924873 D1 DE 69924873D1 DE 69924873 T DE69924873 T DE 69924873T DE 69924873 T DE69924873 T DE 69924873T DE 69924873 D1 DE69924873 D1 DE 69924873D1
- Authority
- DE
- Germany
- Prior art keywords
- methods
- genes
- enzymes
- cytochrom
- monooxygenase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 7
- 238000000034 method Methods 0.000 title abstract 5
- 241000222178 Candida tropicalis Species 0.000 title abstract 2
- 102000005297 Cytochrome P-450 CYP4A Human genes 0.000 title abstract 2
- 108010081498 Cytochrome P-450 CYP4A Proteins 0.000 title abstract 2
- 108010074633 Mixed Function Oxygenases Proteins 0.000 title 1
- 102000008109 Mixed Function Oxygenases Human genes 0.000 title 1
- 108010045510 NADPH-Ferrihemoprotein Reductase Proteins 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000790 Enzymes Proteins 0.000 abstract 3
- 102000004190 Enzymes Human genes 0.000 abstract 3
- 102000002004 Cytochrome P-450 Enzyme System Human genes 0.000 abstract 2
- 108010015742 Cytochrome P-450 Enzyme System Proteins 0.000 abstract 2
- ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 abstract 2
- 102000004316 Oxidoreductases Human genes 0.000 abstract 2
- 108090000854 Oxidoreductases Proteins 0.000 abstract 2
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 abstract 2
- OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
- C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
- C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
- C07K14/40—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Candida
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10309998P | 1998-10-05 | 1998-10-05 | |
| US103099P | 1998-10-05 | ||
| US12355599P | 1999-03-10 | 1999-03-10 | |
| US123555P | 1999-03-10 | ||
| PCT/US1999/020797 WO2000020566A2 (en) | 1998-10-05 | 1999-09-10 | Cytochrome p450 monooxygenase and nadph cytochrome p450 oxidoreductase genes and proteins related to the omega hydroxylase complex of candida tropicalis and methods relating thereto |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69924873D1 true DE69924873D1 (de) | 2005-05-25 |
| DE69924873T2 DE69924873T2 (de) | 2006-03-02 |
Family
ID=26800090
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69941604T Expired - Lifetime DE69941604D1 (de) | 1998-10-05 | 1999-09-10 | Cytochrom P450 Monooxygenase Gen und Protein des Omega-Hydroxylase-Komplexes von Candida Tropicalis und darauf bezogene Verfahren |
| DE69924873T Expired - Lifetime DE69924873T2 (de) | 1998-10-05 | 1999-09-10 | Cytochrom p450 monooxygenase und nadph-cytochrom p450-oxidoreduktase gene und proteine mit bezug zum omega-hydroxylase-komplex in candida tropicalis und dazu gehörigen verfahren |
| DE69941603T Expired - Lifetime DE69941603D1 (de) | 1998-10-05 | 1999-09-10 | Cytochrom P450 Monooxygenase Gen und Protein des omega-Hydroxylase-Komplexes von Candida tropicalis und darauf bezogene Verfahren |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69941604T Expired - Lifetime DE69941604D1 (de) | 1998-10-05 | 1999-09-10 | Cytochrom P450 Monooxygenase Gen und Protein des Omega-Hydroxylase-Komplexes von Candida Tropicalis und darauf bezogene Verfahren |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69941603T Expired - Lifetime DE69941603D1 (de) | 1998-10-05 | 1999-09-10 | Cytochrom P450 Monooxygenase Gen und Protein des omega-Hydroxylase-Komplexes von Candida tropicalis und darauf bezogene Verfahren |
Country Status (7)
| Country | Link |
|---|---|
| EP (12) | EP1148137A3 (de) |
| AT (3) | ATE447028T1 (de) |
| AU (1) | AU5917799A (de) |
| CA (2) | CA2653985A1 (de) |
| DE (3) | DE69941604D1 (de) |
| ES (1) | ES2243075T3 (de) |
| WO (1) | WO2000020566A2 (de) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE60029232T2 (de) | 1999-09-30 | 2007-05-31 | Cognis Ip Management Gmbh | Verbessertes fermentationsverfahren |
| US20020061566A1 (en) | 2000-03-20 | 2002-05-23 | Eirich L. Dudley | Biooxidation capabilities of candida sp |
| US6660505B2 (en) | 2000-06-22 | 2003-12-09 | Cognis Corporation | Isolation of carboxylic acids from fermentation broth |
| US6673613B2 (en) * | 2000-07-26 | 2004-01-06 | Cognis Corporation | Use of CYP52A2A promoter to increase gene expression in yeast |
| AU2002335822A1 (en) * | 2001-10-12 | 2003-04-22 | Cognis Corporation | Production of a highly active, soluble form of the cytochrome p450 reductase (cpr a) from candida tropicalis |
| WO2003100013A2 (en) | 2002-05-23 | 2003-12-04 | Cognis Corporation | NON-REVERTIBLE β-OXIDATION BLOCKED CANDIDA TROPICALIS |
| US6777213B2 (en) | 2002-10-29 | 2004-08-17 | Cognis Corporation | Isolation of carboxylic acids from fermentation broth |
| CN100591754C (zh) * | 2005-10-14 | 2010-02-24 | 中国科学院大连化学物理研究所 | 一种共表达cyp和cpr的酵母系统 |
| DE102007060705A1 (de) | 2007-12-17 | 2009-06-18 | Evonik Degussa Gmbh | ω-Aminocarbonsäuren oder ihre Lactame, herstellende, rekombinante Zellen |
| DE102009046626A1 (de) | 2009-11-11 | 2011-05-12 | Evonik Degussa Gmbh | Candida tropicalis Zellen und deren Verwendung |
| DE102010015807A1 (de) | 2010-04-20 | 2011-10-20 | Evonik Degussa Gmbh | Biokatalytisches Oxidationsverfahren mit alkL-Genprodukt |
| EP2944697A1 (de) | 2014-05-13 | 2015-11-18 | Evonik Degussa GmbH | Verfahren zur Herstellung von Nylon |
| CN108473967B (zh) | 2015-12-17 | 2022-03-29 | 赢创运营有限公司 | 基因修饰的产乙酸细胞 |
| JP2019523271A (ja) | 2016-07-27 | 2019-08-22 | エボニック デグサ ゲーエムベーハーＥｖｏｎｉｋ Ｄｅｇｕｓｓａ ＧｍｂＨ | Ｎ−アセチルホモセリン |
| US20200010862A1 (en) * | 2018-07-06 | 2020-01-09 | Cathay Biotech Inc. | Long-chain dibasic acid with low content of fatty acid impurity and a method of producing the same |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1365689A (en) | 1970-12-26 | 1974-09-04 | Hasegawa T Co Ltd | Process for the production of carboxylic acids |
| DE2140133C2 (de) | 1971-08-10 | 1984-08-09 | Ajinomoto Co., Inc., Tokio | Verfahren zur mikrobiologischen Herstellung von α,ω-n-Alkandicarbonsäuren |
| JPS5543759B2 (de) | 1972-06-28 | 1980-11-07 | ||
| US3975234A (en) | 1975-07-03 | 1976-08-17 | Phillips Petroleum Company | Microbial production of dicarboxylic acids |
| JPS5946598B2 (ja) | 1977-12-14 | 1984-11-13 | 株式会社バイオリサ−チセンタ− | 微生物による長鎖脂肪酸類の製造法 |
| JPS5539778A (en) | 1978-09-14 | 1980-03-19 | Baiorisaac Center:Kk | Microbial preparation of carboxylic acid composed mainly of omega,omega-1-dihydroxy acid |
| JPS5592691A (en) | 1978-12-28 | 1980-07-14 | Baiorisaac Center:Kk | Purification of dicarboxylic acid produced by fermentation |
| US4339536A (en) | 1979-06-08 | 1982-07-13 | Nippon Mining Co., Ltd. | Process for the preparation of long-chain dicarboxylic acids by fermentation |
| JPS5765194A (en) | 1980-10-09 | 1982-04-20 | Daicel Chem Ind Ltd | Microbial preparation of unsaturated dicarboxylic acid |
| US5254466A (en) | 1989-11-06 | 1993-10-19 | Henkel Research Corporation | Site-specific modification of the candida tropicals genome |
| WO1991014781A1 (en) * | 1990-03-19 | 1991-10-03 | Henkel Research Corporation | METHOD FOR INCREASING THE OMEGA-HYDROXYLASE ACTIVITY IN $i(CANDIDA TROPICALIS) |
| US6331420B1 (en) | 1998-05-01 | 2001-12-18 | C. Ron Wilson | Cytochrome P450 monooxygenase and NADPH cytochrome P450 oxidoreductase genes and proteins related to the omega hydroxylase complex of Candida tropicalis and methods relating thereto |
| US6503734B1 (en) | 2000-07-26 | 2003-01-07 | Cognis Corporation | Cytochrome b5 gene and protein of Candida tropicalis and methods relating thereto |
| US7388084B2 (en) * | 2002-08-16 | 2008-06-17 | Cognis Corporation | Promoter motifs in Candida tropicalis |
| DE102007042592A1 (de) | 2007-09-07 | 2009-03-26 | Siemens Medical Instruments Pte. Ltd. | Übertragungseinrichtung für eine Hörvorrichtung mit Folienleiterschirmung und eigengeschirmte Spule |
-
1999
- 1999-09-10 ES ES99946862T patent/ES2243075T3/es not_active Expired - Lifetime
- 1999-09-10 AT AT01116195T patent/ATE447028T1/de not_active IP Right Cessation
- 1999-09-10 EP EP01116363A patent/EP1148137A3/de not_active Withdrawn
- 1999-09-10 AT AT99946862T patent/ATE293694T1/de active
- 1999-09-10 AT AT01116255T patent/ATE447029T1/de not_active IP Right Cessation
- 1999-09-10 EP EP01116195A patent/EP1148131B1/de not_active Expired - Lifetime
- 1999-09-10 EP EP01116253A patent/EP1162268A3/de not_active Withdrawn
- 1999-09-10 DE DE69941604T patent/DE69941604D1/de not_active Expired - Lifetime
- 1999-09-10 EP EP01116362A patent/EP1148136B1/de not_active Expired - Lifetime
- 1999-09-10 EP EP01116518A patent/EP1148143A3/de not_active Withdrawn
- 1999-09-10 EP EP01116519A patent/EP1148138A3/de not_active Withdrawn
- 1999-09-10 CA CA002653985A patent/CA2653985A1/en not_active Abandoned
- 1999-09-10 EP EP01116201A patent/EP1148132A3/de not_active Withdrawn
- 1999-09-10 WO PCT/US1999/020797 patent/WO2000020566A2/en not_active Ceased
- 1999-09-10 CA CA2349868A patent/CA2349868C/en not_active Expired - Fee Related
- 1999-09-10 EP EP99946862A patent/EP1135506B1/de not_active Expired - Lifetime
- 1999-09-10 EP EP01116254A patent/EP1148133A3/de not_active Withdrawn
- 1999-09-10 DE DE69924873T patent/DE69924873T2/de not_active Expired - Lifetime
- 1999-09-10 EP EP01116361A patent/EP1148135A3/de not_active Withdrawn
- 1999-09-10 EP EP01116194A patent/EP1148130A3/de not_active Ceased
- 1999-09-10 DE DE69941603T patent/DE69941603D1/de not_active Expired - Lifetime
- 1999-09-10 AU AU59177/99A patent/AU5917799A/en not_active Abandoned
- 1999-09-10 EP EP01116255A patent/EP1148134B1/de not_active Expired - Lifetime
Also Published As
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |