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DE69725901D1 - Methode zur bestimmung von proteolytischen enzyme unter benutzung von fluoreszenzgelöschten substraten - Google Patents

Methode zur bestimmung von proteolytischen enzyme unter benutzung von fluoreszenzgelöschten substraten

Info

Publication number
DE69725901D1
DE69725901D1 DE69725901T DE69725901T DE69725901D1 DE 69725901 D1 DE69725901 D1 DE 69725901D1 DE 69725901 T DE69725901 T DE 69725901T DE 69725901 T DE69725901 T DE 69725901T DE 69725901 D1 DE69725901 D1 DE 69725901D1
Authority
DE
Germany
Prior art keywords
extinguished
fluorescence
substrates
proteolytic enzymes
determining proteolytic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69725901T
Other languages
English (en)
Other versions
DE69725901T2 (de
Inventor
Koppele Maria Te
Bob Beekman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO
Original Assignee
Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO filed Critical Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO
Application granted granted Critical
Publication of DE69725901D1 publication Critical patent/DE69725901D1/de
Publication of DE69725901T2 publication Critical patent/DE69725901T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4725—Proteoglycans, e.g. aggreccan
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96486—Metalloendopeptidases (3.4.24)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DE69725901T 1996-01-04 1997-01-02 Methode zur bestimmung von proteolytischen enzymen unter benutzung von fluoreszenzgelöschten substraten Expired - Fee Related DE69725901T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP96200017 1996-01-04
EP96200017 1996-01-04
PCT/NL1997/000002 WO1997025437A1 (en) 1996-01-04 1997-01-02 Method for assaying proteolytic enzymes using fluorescence-quenched substrates

Publications (2)

Publication Number Publication Date
DE69725901D1 true DE69725901D1 (de) 2003-12-04
DE69725901T2 DE69725901T2 (de) 2004-09-09

Family

ID=8223570

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69725901T Expired - Fee Related DE69725901T2 (de) 1996-01-04 1997-01-02 Methode zur bestimmung von proteolytischen enzymen unter benutzung von fluoreszenzgelöschten substraten

Country Status (5)

Country Link
US (1) US6127139A (de)
EP (1) EP1019528B1 (de)
JP (1) JP2000503533A (de)
DE (1) DE69725901T2 (de)
WO (1) WO1997025437A1 (de)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
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ATE203545T1 (de) * 1996-04-24 2001-08-15 Medivir Uk Ltd Substrate und inhibitoren von proteolytischen enzymen
WO1997043438A1 (en) * 1996-05-14 1997-11-20 University Of Manitoba Solid-phase activity assay for biologically active substance
AU8513198A (en) 1997-07-25 1999-02-16 Du Pont Pharmaceuticals Company Aggrecan degrading metallo proteases
US6908770B1 (en) 1998-07-16 2005-06-21 Board Of Regents, The University Of Texas System Fluid based analysis of multiple analytes by a sensor array
WO2000005256A1 (en) * 1998-07-24 2000-02-03 Du Pont Pharmaceuticals Company Assays and peptide substrate for determining aggrecan degrading metallo protease activity
DE19903576C2 (de) * 1999-01-29 2001-02-22 Bodenseewerk Perkin Elmer Co Quantitative Bestimmung von Analyten in einem heterogenen System
US6602702B1 (en) 1999-07-16 2003-08-05 The University Of Texas System Detection system based on an analyte reactive particle
US7022517B1 (en) 1999-07-16 2006-04-04 Board Of Regents, The University Of Texas System Method and apparatus for the delivery of samples to a chemical sensor array
AU2001233200A1 (en) * 2000-01-31 2001-08-07 Board Of Regents, The University Of Texas System Portable sensor array system
US6600057B2 (en) 2000-12-29 2003-07-29 Kimberly-Clark Worldwide, Inc. Matrix metalloproteinase inhibitors
US7041787B2 (en) 2000-12-29 2006-05-09 Kimberly-Clark Worldwide, Inc. Design and use of advanced zinc chelating peptides to regulate matrix metalloproteinases
US20020160363A1 (en) * 2001-01-31 2002-10-31 Mcdevitt John T. Magnetic-based placement and retention of sensor elements in a sensor array
US20030003436A1 (en) * 2001-02-05 2003-01-02 Willson C. Grant Use of mesoscale self-assembly and recognition to effect delivery of sensing reagent for arrayed sensors
US6979530B2 (en) * 2001-05-21 2005-12-27 Applera Corporation Peptide conjugates and fluorescence detection methods for intracellular caspase assay
US6906036B2 (en) 2001-08-16 2005-06-14 Kimberly-Clark Worldwide, Inc. Anti-aging and wound healing compounds
US7071164B2 (en) * 2001-08-16 2006-07-04 Kimberly-Clark Worldwide, Inc. Anti-cancer and wound healing compounds
US7186693B2 (en) 2001-08-16 2007-03-06 Kimberly - Clark Worldwide, Inc. Metalloproteinase inhibitors for wound healing
US7094754B2 (en) 2001-08-16 2006-08-22 Kimberly-Clark Worldwide, Inc. Anti-aging and wound healing compounds
US20030119073A1 (en) * 2001-12-21 2003-06-26 Stephen Quirk Sensors and methods of detection for proteinase enzymes
WO2003090605A2 (en) 2002-04-26 2003-11-06 Board Of Regents, The University Of Texas System Method and system for the detection of cardiac risk factors
KR20050034642A (ko) * 2002-05-10 2005-04-14 파마시아 코퍼레이션 펩티드 화합물 및 프로테아제 기질로서의 그의 용도
WO2003102544A2 (en) * 2002-05-31 2003-12-11 Cognosci, Inc. Assays for measuring matrix metalloproteinase activities
GB2390157B (en) * 2002-06-25 2005-09-28 Johnson & Johnson Medical Ltd Wound fluid collecting and indicating device
EP1546367A4 (de) * 2002-07-24 2006-08-16 Univ Texas Einfang und nachweis von mikroben mit membranverfahren
US7148194B2 (en) 2002-12-30 2006-12-12 Kimberly-Clark Worldwide, Inc. Method to increase fibronectin
PL215163B1 (pl) * 2003-01-31 2013-10-31 Ethicon Inc Sposób wykrywania drobnoustroju w próbce, biosensor do wykrywania drobnoustroju w próbce i zestaw do wykrywania zakazenia rany
WO2004097371A2 (en) * 2003-04-25 2004-11-11 Board Of Regents, The University Of Texas System System and method for the detection of analytes
US7651850B2 (en) * 2003-05-16 2010-01-26 Board Of Regents, The University Of Texas System Image and part recognition technology
US9317922B2 (en) 2003-05-16 2016-04-19 Board Of Regents The University Of Texas System Image and part recognition technology
US7189700B2 (en) * 2003-06-20 2007-03-13 Kimberly-Clark Worldwide, Inc. Anti-chrondrosarcoma compounds
EP1644515A2 (de) * 2003-06-23 2006-04-12 NLCPharma, Inc. Enzymatischer diagnostischer test für sars und andere viruserkrankungen
AU2004300168A1 (en) 2003-12-11 2005-06-30 Board Of Regents, The University Of Texas System Method and system for the analysis of saliva using a sensor array
US8101431B2 (en) 2004-02-27 2012-01-24 Board Of Regents, The University Of Texas System Integration of fluids and reagents into self-contained cartridges containing sensor elements and reagent delivery systems
US20060257854A1 (en) * 2004-02-27 2006-11-16 Mcdevitt John T Membrane assay system including preloaded particles
US20060257941A1 (en) * 2004-02-27 2006-11-16 Mcdevitt John T Integration of fluids and reagents into self-contained cartridges containing particle and membrane sensor elements
US20060257991A1 (en) * 2004-02-27 2006-11-16 Mcdevitt John T Integration of fluids and reagents into self-contained cartridges containing particle-based sensor elements and membrane-based sensor elements
US8105849B2 (en) 2004-02-27 2012-01-31 Board Of Regents, The University Of Texas System Integration of fluids and reagents into self-contained cartridges containing sensor elements
US7781226B2 (en) * 2004-02-27 2010-08-24 The Board Of Regents Of The University Of Texas System Particle on membrane assay system
AU2006309284B2 (en) 2005-05-31 2012-08-02 Board Of Regents, The University Of Texas System Methods and compositions related to determination and use of white blood cell counts
KR101633473B1 (ko) * 2014-05-12 2016-06-27 부산대학교 산학협력단 단백질분해효소 검출센서
WO2016135882A1 (ja) 2015-02-25 2016-09-01 オリンパス株式会社 十二指腸液試料の膵液由来成分検出用試料としての適性評価方法
GB201504778D0 (en) 2015-03-20 2015-05-06 Univ Edinburgh Optical probes for matrix metalloproteinases
CN111896504A (zh) * 2020-06-17 2020-11-06 山东省医学科学院基础医学研究所 一种新型冠状病毒Mpro蛋白酶靶点药物筛选试剂盒及其应用
CN113045672B (zh) * 2021-02-09 2022-02-01 甘肃省科学院传感技术研究所 一种用于检测基质金属蛋白酶-2的磁性荧光复合探针及其制备方法和应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0428000A1 (de) * 1989-11-03 1991-05-22 Abbott Laboratories Fluorogene Substrate zum Nachweis von proteolytischer Enzymaktivität
FR2658519B1 (fr) * 1990-02-19 1995-07-07 Serbio Substrats peptidiques pour identification du facteur xa.
DK93990D0 (da) * 1990-04-17 1990-04-17 Carlsberg As Fluorogene peptider og deres anvendelse ved bestemmelse af enzymatisk aktivitet
US5235039A (en) * 1991-06-10 1993-08-10 Eli Lilly And Company Substrates for hiv protease
US5427954A (en) * 1992-04-29 1995-06-27 Shriner's Hospitals For Crippled Children Compositions and methods for detection and treatment of human osteoarthritis
GB9310978D0 (en) * 1993-05-27 1993-07-14 Zeneca Ltd Compounds

Also Published As

Publication number Publication date
WO1997025437A1 (en) 1997-07-17
JP2000503533A (ja) 2000-03-28
EP1019528A1 (de) 2000-07-19
DE69725901T2 (de) 2004-09-09
US6127139A (en) 2000-10-03
EP1019528B1 (de) 2003-10-29

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Legal Events

Date Code Title Description
8339 Ceased/non-payment of the annual fee