DE69535705D1 - FUNCTIONAL TRACKING AND / OR EXPRESSION VECTORS IN MYCOBACTERIA - Google Patents
FUNCTIONAL TRACKING AND / OR EXPRESSION VECTORS IN MYCOBACTERIAInfo
- Publication number
- DE69535705D1 DE69535705D1 DE69535705T DE69535705T DE69535705D1 DE 69535705 D1 DE69535705 D1 DE 69535705D1 DE 69535705 T DE69535705 T DE 69535705T DE 69535705 T DE69535705 T DE 69535705T DE 69535705 D1 DE69535705 D1 DE 69535705D1
- Authority
- DE
- Germany
- Prior art keywords
- mycobacteria
- vector
- ntseq
- marker
- isolated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000013604 expression vector Substances 0.000 title abstract 2
- 239000013598 vector Substances 0.000 abstract 5
- 239000003550 marker Substances 0.000 abstract 4
- 238000010367 cloning Methods 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 238000012216 screening Methods 0.000 abstract 2
- 230000028327 secretion Effects 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 1
- 241000701533 Escherichia virus T4 Species 0.000 abstract 1
- 241000186359 Mycobacterium Species 0.000 abstract 1
- 108700008625 Reporter Genes Proteins 0.000 abstract 1
- 108091081024 Start codon Proteins 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 238000012512 characterization method Methods 0.000 abstract 1
- 238000001976 enzyme digestion Methods 0.000 abstract 1
- 230000014726 immortalization of host cell Effects 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 108091008146 restriction endonucleases Proteins 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
- 238000011144 upstream manufacturing Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
New recombinant screening, cloning and/or expression vectors (V1 and V2), able to replicate in mycobacteria contain: (i) a replicon functional in mycobacteria; (ii) a selection marker; (iii) a reporter cassette comprising: (a) a multiple cloning site (polylinker, PL); (b) a transcription terminator active in mycobacteria (pref. coliphage T4 terminator) and located upstream of PL, and for vector V1: (c) a nucleotide sequence (ntseq) derived from a gene coding for an expression marker, or protein export and/or secretion marker, the ntseq lacking an initiation codon and regulation sequences; or for vector V2: (c') a reporter gene whose expression can be analysed in the presence of different regulatory sequences. Pref. vector V1 further comprises a ntseq (A) from a mycobacterium, cloned into PL. In a new screening method, ntseq (A), obtained by restriction enzyme digestion or by in vitro amplification, is inserted into the PL of vector V1 and the resulting vector is opt. replicated before being used for transformation of host cells. DNA is isolated from positive transformants (i.e. cells expressing the export/secretion marker) and subcloned. Inserts from the subclones are isolated to allow characterisation of the mycobacteria sequences they contain. Ntseqs isolated by this method are claimed, per se.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9410585A FR2724183B1 (en) | 1994-09-02 | 1994-09-02 | FUNCTIONAL SCREENING AND / OR EXPRESSION VECTORS IN MYCOBACTERIA - USE FOR IDENTIFICATION AND EXPRESSION OF EXPORTED POLYPEPTIDES |
| FR9410585 | 1994-09-02 | ||
| PCT/FR1995/001133 WO1996007745A2 (en) | 1994-09-02 | 1995-08-30 | Mycobacteria functional screening and/or expression vectors |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69535705D1 true DE69535705D1 (en) | 2008-03-27 |
| DE69535705T2 DE69535705T2 (en) | 2009-04-16 |
Family
ID=9466676
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69535705T Expired - Lifetime DE69535705T2 (en) | 1994-09-02 | 1995-08-30 | FUNCTIONAL SURVEYS AND / OR EXPRESSION VECTORS IN MYCOBACTERIA |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US6248581B1 (en) |
| EP (2) | EP1396546A3 (en) |
| JP (1) | JPH10504966A (en) |
| AT (1) | ATE386125T1 (en) |
| CA (1) | CA2197717C (en) |
| DE (1) | DE69535705T2 (en) |
| DK (1) | DK0770138T3 (en) |
| ES (1) | ES2301162T3 (en) |
| FR (1) | FR2724183B1 (en) |
| PT (1) | PT770138E (en) |
| WO (1) | WO1996007745A2 (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZA964896B (en) * | 1995-06-07 | 1997-01-08 | Connaught Lab | Expression of lipoproteins |
| ATE396269T1 (en) * | 1996-07-26 | 2008-06-15 | Pasteur Institut | DESATURASE ANTIGEN FROM MYCOBACTERIUM TUBERCOLOSIS |
| FR2766495B1 (en) * | 1997-07-22 | 2001-07-13 | Pasteur Institut | MYCOBACTERIUM STRAIN IN WHICH THE ERP GENE IS MODIFIED AND THE VACCINE COMPOSITION CONTAINING THE SAME |
| FR2767337B1 (en) * | 1997-08-14 | 2002-07-05 | Pasteur Institut | NUCLEIC SEQUENCES OF POLYPEPTIDES EXPORTED FROM MYCOBACTERI ES, VECTORS COMPRISING THEM AND APPLICATIONS TO DIAGNOSIS AND THE PREVENTION OF TUBERCULOSIS |
| FR2767336B1 (en) * | 1997-08-14 | 2001-05-18 | Pasteur Institut | RECOMBINANT VECTORS, POLYNUCLEOTIDE ENCODING A 12KD POLYPEPTIDE PD428 OF MYCOBACTERIA BELONGING TO THE MYCOBACTERIUM TUBERCULOSIS COMPLEX AND APPLICATIONS TO DIAGNOSIS AND THE PREVENTION OF TUBERCULOSIS |
| AT410217B (en) * | 2000-06-15 | 2003-03-25 | Cistem Biotechnologies Gmbh | VECTOR AND A METHOD FOR THE EXPRESSION AND SELECTION OF RANDOMIZED PEPTIDE SEQUENCES |
| KR101111000B1 (en) * | 2003-11-12 | 2012-02-15 | 인터디지탈 테크날러지 코포레이션 | System for application server autonomous access across different types of access technology networks |
| TWI285861B (en) * | 2004-05-21 | 2007-08-21 | Sanyo Electric Co | Display device |
| EP2163635B1 (en) | 2004-08-02 | 2013-05-22 | BASF Plant Science GmbH | Method for isolation of transcription termination sequences |
| US20060134009A1 (en) * | 2004-12-16 | 2006-06-22 | Daniel Deaver | Low dose corticosteroid powders for inhalation |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4906742A (en) * | 1986-07-31 | 1990-03-06 | Whitehead Institute For Biomedical Research | Encoding antigens of M. Leprae |
-
1994
- 1994-09-02 FR FR9410585A patent/FR2724183B1/en not_active Expired - Fee Related
-
1995
- 1995-08-30 CA CA2197717A patent/CA2197717C/en not_active Expired - Fee Related
- 1995-08-30 EP EP03021544A patent/EP1396546A3/en not_active Withdrawn
- 1995-08-30 JP JP8509250A patent/JPH10504966A/en active Pending
- 1995-08-30 WO PCT/FR1995/001133 patent/WO1996007745A2/en not_active Ceased
- 1995-08-30 PT PT95929147T patent/PT770138E/en unknown
- 1995-08-30 US US08/793,701 patent/US6248581B1/en not_active Expired - Fee Related
- 1995-08-30 DK DK95929147T patent/DK0770138T3/en active
- 1995-08-30 ES ES95929147T patent/ES2301162T3/en not_active Expired - Lifetime
- 1995-08-30 EP EP95929147A patent/EP0770138B1/en not_active Expired - Lifetime
- 1995-08-30 DE DE69535705T patent/DE69535705T2/en not_active Expired - Lifetime
- 1995-08-30 AT AT95929147T patent/ATE386125T1/en not_active IP Right Cessation
-
2000
- 2000-05-26 US US09/579,264 patent/US6565855B1/en not_active Expired - Fee Related
-
2003
- 2003-01-15 US US10/342,232 patent/US7601350B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| DE69535705T2 (en) | 2009-04-16 |
| US6565855B1 (en) | 2003-05-20 |
| DK0770138T3 (en) | 2008-06-09 |
| US6248581B1 (en) | 2001-06-19 |
| WO1996007745A2 (en) | 1996-03-14 |
| FR2724183A1 (en) | 1996-03-08 |
| CA2197717C (en) | 2010-10-26 |
| ATE386125T1 (en) | 2008-03-15 |
| EP1396546A3 (en) | 2004-06-02 |
| JPH10504966A (en) | 1998-05-19 |
| EP0770138A2 (en) | 1997-05-02 |
| EP1396546A2 (en) | 2004-03-10 |
| PT770138E (en) | 2008-04-29 |
| WO1996007745A3 (en) | 1996-07-11 |
| CA2197717A1 (en) | 1996-03-14 |
| US7601350B2 (en) | 2009-10-13 |
| FR2724183B1 (en) | 1997-04-11 |
| ES2301162T3 (en) | 2008-06-16 |
| EP0770138B1 (en) | 2008-02-13 |
| US20060127959A1 (en) | 2006-06-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |