DE60139663D1 - Verfahren zur detektion von polynukleotidkinase und deren verwendung als markierung - Google Patents
Verfahren zur detektion von polynukleotidkinase und deren verwendung als markierungInfo
- Publication number
- DE60139663D1 DE60139663D1 DE60139663T DE60139663T DE60139663D1 DE 60139663 D1 DE60139663 D1 DE 60139663D1 DE 60139663 T DE60139663 T DE 60139663T DE 60139663 T DE60139663 T DE 60139663T DE 60139663 D1 DE60139663 D1 DE 60139663D1
- Authority
- DE
- Germany
- Prior art keywords
- polynucleotide kinase
- oligonucleotide
- methods
- phosphorylation
- oligonucleotides
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title abstract 5
- 108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 title abstract 4
- 102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 title abstract 4
- 238000001514 detection method Methods 0.000 title 1
- 239000003550 marker Substances 0.000 title 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 6
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 2
- 230000026731 phosphorylation Effects 0.000 abstract 2
- 238000006366 phosphorylation reaction Methods 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- 239000012491 analyte Substances 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 239000010452 phosphate Substances 0.000 abstract 1
- 238000011896 sensitive detection Methods 0.000 abstract 1
- 230000009870 specific binding Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US19162100P | 2000-03-23 | 2000-03-23 | |
| PCT/US2001/008621 WO2001071037A1 (en) | 2000-03-23 | 2001-03-23 | Methods of detecting polynucleotide kinase and its use as a label |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE60139663D1 true DE60139663D1 (de) | 2009-10-08 |
Family
ID=22706206
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60139663T Expired - Fee Related DE60139663D1 (de) | 2000-03-23 | 2001-03-23 | Verfahren zur detektion von polynukleotidkinase und deren verwendung als markierung |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US7129045B2 (de) |
| EP (1) | EP1180169B1 (de) |
| JP (1) | JP2003527866A (de) |
| AT (1) | ATE440964T1 (de) |
| AU (1) | AU785111C (de) |
| CA (1) | CA2374393A1 (de) |
| DE (1) | DE60139663D1 (de) |
| WO (1) | WO2001071037A1 (de) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005529606A (ja) * | 2002-06-12 | 2005-10-06 | データスコープ・インベストメント・コーポレイション | ポリマー標識分子 |
| KR101019293B1 (ko) * | 2005-11-04 | 2011-03-07 | 어플라이드 머티어리얼스, 인코포레이티드 | 플라즈마-강화 원자층 증착 장치 및 방법 |
| JP2007275006A (ja) * | 2006-04-10 | 2007-10-25 | Hitachi High-Technologies Corp | 核酸検出用プローブ作製法 |
| US20120269728A1 (en) * | 2006-08-02 | 2012-10-25 | Antara Biosciences Inc. | Methods and compositions for detecting one or more target agents using tracking components |
| JP5500985B2 (ja) * | 2007-03-28 | 2014-05-21 | 悦朗 伊藤 | タンパク質および核酸の超高感度測定法 |
| US10208358B2 (en) * | 2014-04-14 | 2019-02-19 | Indiana University Reasearch and Technology Corporation | Generalizable assay for virus capsid assembly |
| GB201612011D0 (en) * | 2016-07-11 | 2016-08-24 | Glaxosmithkline Ip Dev Ltd | Novel processes for the production of oligonucleotides |
| CN112710710B (zh) * | 2020-12-08 | 2022-02-18 | 云南民族大学 | 基于磁性纳米材料和生物信号放大技术测定t4多聚核苷酸激酶活性的方法 |
| CN114018890B (zh) * | 2021-11-11 | 2024-05-03 | 天津师范大学 | 一种检测高盐高蛋白质生物样品中多聚核苷酸激酶的方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4883750A (en) * | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
| US5512439A (en) * | 1988-11-21 | 1996-04-30 | Dynal As | Oligonucleotide-linked magnetic particles and uses thereof |
| US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5599921A (en) * | 1991-05-08 | 1997-02-04 | Stratagene | Oligonucleotide families useful for producing primers |
| US5559921A (en) * | 1994-06-24 | 1996-09-24 | Sumitomo Electric Industries, Ltd. | Single mode optical fiber |
| US5853974A (en) * | 1995-06-07 | 1998-12-29 | Chiron Corporation | Enhancement of alkaline phosphatase with SDS in chemiluminescent substrates |
| US5888731A (en) * | 1995-08-30 | 1999-03-30 | Visible Genetics Inc. | Method for identification of mutations using ligation of multiple oligonucleotide probes |
| US5998175A (en) * | 1998-07-24 | 1999-12-07 | Lumigen, Inc. | Methods of synthesizing and amplifying polynucleotides by ligation of multiple oligomers |
-
2001
- 2001-03-23 US US09/979,215 patent/US7129045B2/en not_active Expired - Fee Related
- 2001-03-23 WO PCT/US2001/008621 patent/WO2001071037A1/en not_active Ceased
- 2001-03-23 AT AT01920491T patent/ATE440964T1/de not_active IP Right Cessation
- 2001-03-23 EP EP01920491A patent/EP1180169B1/de not_active Expired - Lifetime
- 2001-03-23 AU AU47538/01A patent/AU785111C/en not_active Ceased
- 2001-03-23 DE DE60139663T patent/DE60139663D1/de not_active Expired - Fee Related
- 2001-03-23 CA CA002374393A patent/CA2374393A1/en not_active Abandoned
- 2001-03-23 JP JP2001569416A patent/JP2003527866A/ja active Pending
-
2006
- 2006-08-11 US US11/502,781 patent/US20070003968A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1180169A4 (de) | 2005-01-12 |
| US20040253585A1 (en) | 2004-12-16 |
| CA2374393A1 (en) | 2001-09-27 |
| AU785111C (en) | 2007-05-03 |
| EP1180169B1 (de) | 2009-08-26 |
| JP2003527866A (ja) | 2003-09-24 |
| US7129045B2 (en) | 2006-10-31 |
| AU785111B2 (en) | 2006-09-21 |
| US20070003968A1 (en) | 2007-01-04 |
| WO2001071037A1 (en) | 2001-09-27 |
| ATE440964T1 (de) | 2009-09-15 |
| EP1180169A1 (de) | 2002-02-20 |
| AU4753801A (en) | 2001-10-03 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |