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DE60032184D1 - Verfahren zur verbesserung der gentransfer-effizienz in pflanzenzellen - Google Patents

Verfahren zur verbesserung der gentransfer-effizienz in pflanzenzellen

Info

Publication number
DE60032184D1
DE60032184D1 DE60032184T DE60032184T DE60032184D1 DE 60032184 D1 DE60032184 D1 DE 60032184D1 DE 60032184 T DE60032184 T DE 60032184T DE 60032184 T DE60032184 T DE 60032184T DE 60032184 D1 DE60032184 D1 DE 60032184D1
Authority
DE
Germany
Prior art keywords
gentransfer
improving
efficiency
plant cells
plant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60032184T
Other languages
English (en)
Other versions
DE60032184T2 (de
Inventor
Yuji Ishida
Yukoh Hiei
Keisuke Kasaoka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japan Tobacco Inc
Original Assignee
Japan Tobacco Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Tobacco Inc filed Critical Japan Tobacco Inc
Application granted granted Critical
Publication of DE60032184D1 publication Critical patent/DE60032184D1/de
Publication of DE60032184T2 publication Critical patent/DE60032184T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
DE60032184T 2000-08-03 2000-08-03 Verfahren zur verbesserung der gentransfer-effizienz in pflanzenzellen Expired - Lifetime DE60032184T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2000/005213 WO2002012520A1 (fr) 1999-06-04 2000-08-03 Procede permettant d'ameliorer l'efficacite du transfert de genes dans des cellules vegetales

Publications (2)

Publication Number Publication Date
DE60032184D1 true DE60032184D1 (de) 2007-01-11
DE60032184T2 DE60032184T2 (de) 2007-10-31

Family

ID=11736322

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60032184T Expired - Lifetime DE60032184T2 (de) 2000-08-03 2000-08-03 Verfahren zur verbesserung der gentransfer-effizienz in pflanzenzellen

Country Status (11)

Country Link
US (2) US7960611B2 (de)
EP (1) EP1306440B1 (de)
KR (1) KR100807434B1 (de)
CN (1) CN1268752C (de)
AU (1) AU785287B2 (de)
CA (1) CA2386125C (de)
DE (1) DE60032184T2 (de)
DK (1) DK1306440T3 (de)
ES (1) ES2277592T3 (de)
PT (1) PT1306440E (de)
WO (1) WO2002012520A1 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2539299C (en) 2003-08-13 2011-11-15 Japan Tobacco Inc. Method of introducing gene into plant material
CN1836040B (zh) 2003-08-13 2010-12-08 日本烟草产业株式会社 通过添加铜离子来使植物转化效率提高的方法
WO2007069301A1 (ja) 2005-12-13 2007-06-21 Japan Tobacco Inc. 粉体を用いて形質転換効率を向上させる方法
CN101668418B (zh) 2007-02-28 2013-03-20 日本烟草产业株式会社 包括用含3,6-二氯-2-甲氧基苯甲酸的共存培养基培养植物组织的共存步骤的提高植物转化效率的方法
JPWO2008105509A1 (ja) 2007-02-28 2010-06-03 日本たばこ産業株式会社 選抜工程を経ないアグロバクテリウム菌による形質転換植物の作成方法
JP2011120478A (ja) 2008-03-31 2011-06-23 Japan Tobacco Inc アグロバクテリウム菌による形質転換植物の作成方法
AU2010278126B2 (en) 2009-07-29 2015-01-22 Kaneka Corporation A method of gene introduction into triticum plant using agrobacterium, and a method of producing transformed triticum plant
JP2013212051A (ja) 2010-07-29 2013-10-17 Japan Tobacco Inc アグロバクテリウム菌を用いた、オオムギ属植物へ遺伝子導入を行う方法およびオオムギ属植物の形質転換植物の作成方法
CN102321667B (zh) * 2011-10-11 2013-03-06 西南大学 农杆菌介导大分子量t-dna转化的毒性辅助载体及其制备方法和应用
CN103278502B (zh) * 2013-04-23 2015-12-23 北京大北农科技集团股份有限公司 启动子活性的检测方法
DE102013020605A1 (de) 2013-12-15 2015-06-18 Kws Saat Ag Selektionsmarker-freies rhizobiaceae-vermitteltes verfahren zur herstellung einer transgenen pflanze der gattung triticum
KR102217467B1 (ko) 2020-11-05 2021-02-19 (주)지플러스생명과학 식물기반 바이오약품 제조를 위한 식물형질전환이 이루어진 기주식물의 재배방법
KR102285637B1 (ko) 2020-11-05 2021-08-04 (주)지플러스생명과학 식물기반 바이오의약품 제조를 위한 식물 형질 전환 방법

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0290799B9 (de) 1983-01-13 2004-09-01 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Transgene dicotyledone Pflanzenzellen und Pflanzen
NL8300699A (nl) * 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; werkwijze voor het produceren van agrobacterium tumefaciens bacterien; stabiele cointegraat plasmiden; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
NL8300698A (nl) * 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
NL8401048A (nl) 1984-04-03 1985-11-01 Rijksuniversiteit Leiden En Pr Werkwijze voor het inbouwen van vreemd dna in het genoom van eenzaadlobbige planten.
DE3926390A1 (de) * 1989-08-10 1991-02-14 Bayer Ag Verwendung von lysozym genen in pflanzen zur resistenzerhoehung
AU643563B2 (en) * 1990-11-01 1993-11-18 Sapporo Breweries Limited Method for preparing transformed plant
JPH04330234A (ja) 1991-03-20 1992-11-18 Japan Tobacco Inc キュウリモザイクウィルス抵抗性トマト及びその作出方法
DE69334225D1 (de) * 1992-07-07 2008-07-31 Japan Tobacco Inc Verfahren zur transformation einer monokotyledon pflanze
US5712112A (en) * 1992-11-04 1998-01-27 National Science Council Of R.O.C. Gene expression system comprising the promoter region of the alpha-amylase genes
CA2148499C (en) 1993-09-03 2006-07-11 Hideaki Saito Method for transforming monocotyledons using scutella of immature embryos
US5731179A (en) * 1993-12-08 1998-03-24 Japan Tobacco Inc. Method for introducing two T-DNAS into plants and vectors therefor
EP0737748A1 (de) * 1995-03-17 1996-10-16 Hoechst NOR-AM AgrEvo Inc. Engiebige Herstellung von transgenen fertilen homozygoten Pflanzen aus frische Mikrospore
JPH10117776A (ja) * 1996-10-22 1998-05-12 Japan Tobacco Inc インディカイネの形質転換方法
JP3312867B2 (ja) * 1996-11-08 2002-08-12 北興化学工業株式会社 植物の形質転換方法および形質転換植物の作出方法
US5981840A (en) * 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
CA2265570A1 (en) 1997-07-23 1999-02-04 Japan Tobacco Inc. Method for selecting transformed cells
US6362393B1 (en) * 1998-09-09 2002-03-26 Northwest Plant Breeding Company Methods for generating doubled haploid plants
JP2002533090A (ja) * 1998-12-23 2002-10-08 ザ、サミュアル、ラバツ、ノゥブル、ファウンデイシャン、インク 植物形質転換法
JP2000342255A (ja) 1999-06-04 2000-12-12 Japan Tobacco Inc 植物細胞への遺伝子導入の効率を向上させる方法
JP3141084B2 (ja) 1999-07-21 2001-03-05 農林水産省農業生物資源研究所長 単子葉植物の超迅速形質転換法
DE69939944D1 (de) 1999-09-30 2009-01-02 Japan Tobacco Inc Vektoren zum transformieren von pflanzen

Also Published As

Publication number Publication date
US20110030100A1 (en) 2011-02-03
US9840714B2 (en) 2017-12-12
CN1268752C (zh) 2006-08-09
AU785287B2 (en) 2006-12-21
KR20020092915A (ko) 2002-12-12
US20110209251A1 (en) 2011-08-25
ES2277592T3 (es) 2007-07-16
US7960611B2 (en) 2011-06-14
EP1306440B1 (de) 2006-11-29
PT1306440E (pt) 2007-02-28
EP1306440A1 (de) 2003-05-02
CN1377420A (zh) 2002-10-30
AU6317900A (en) 2002-02-18
DE60032184T2 (de) 2007-10-31
CA2386125C (en) 2010-07-13
CA2386125A1 (en) 2002-02-14
KR100807434B1 (ko) 2008-02-25
DK1306440T3 (da) 2007-04-02
WO2002012520A1 (fr) 2002-02-14
EP1306440A4 (de) 2004-07-14

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