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DE60029927D1 - Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der in vitro nucleinsäuresynthese und amplifikation - Google Patents

Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der in vitro nucleinsäuresynthese und amplifikation

Info

Publication number
DE60029927D1
DE60029927D1 DE60029927T DE60029927T DE60029927D1 DE 60029927 D1 DE60029927 D1 DE 60029927D1 DE 60029927 T DE60029927 T DE 60029927T DE 60029927 T DE60029927 T DE 60029927T DE 60029927 D1 DE60029927 D1 DE 60029927D1
Authority
DE
Germany
Prior art keywords
thermostatic
nucleic acid
amplification
enzymic
accuracy
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60029927T
Other languages
English (en)
Other versions
DE60029927T2 (de
Inventor
Waltraud Ankenbauer
Frank Laue
Harald Sobek
Michael Greif
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Publication of DE60029927D1 publication Critical patent/DE60029927D1/de
Application granted granted Critical
Publication of DE60029927T2 publication Critical patent/DE60029927T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE60029927T 1999-09-28 2000-09-27 Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der in vitro nucleinsäuresynthese und amplifikation Expired - Lifetime DE60029927T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP99119268 1999-09-28
EP99119268A EP1088891B1 (de) 1999-09-28 1999-09-28 Thermostabiles Enzym welches die Genauigkeit thermostabiler DNA Polymerasen erhöht - zur Verbesserung der Nucleinsäuresynthese und in vitro Amplifikation
PCT/EP2000/009423 WO2001023583A2 (en) 1999-09-28 2000-09-27 Thermostable enzyme promoting the fidelity of thermostable dna polymerases- for improvement of nucleic acid synthesis and amplification in vitro

Publications (2)

Publication Number Publication Date
DE60029927D1 true DE60029927D1 (de) 2006-09-21
DE60029927T2 DE60029927T2 (de) 2007-03-08

Family

ID=8239082

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69923180T Expired - Fee Related DE69923180T2 (de) 1999-09-28 1999-09-28 Thermostabiles Enzym welches die Genauigkeit thermostabiler DNA Polymerasen erhöht - zur Verbesserung der Nucleinsäuresynthese und in vitro Amplifikation
DE60029927T Expired - Lifetime DE60029927T2 (de) 1999-09-28 2000-09-27 Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der in vitro nucleinsäuresynthese und amplifikation

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE69923180T Expired - Fee Related DE69923180T2 (de) 1999-09-28 1999-09-28 Thermostabiles Enzym welches die Genauigkeit thermostabiler DNA Polymerasen erhöht - zur Verbesserung der Nucleinsäuresynthese und in vitro Amplifikation

Country Status (14)

Country Link
US (2) US7030220B1 (de)
EP (2) EP1088891B1 (de)
JP (1) JP3655240B2 (de)
CN (1) CN1185347C (de)
AT (2) ATE286981T1 (de)
AU (1) AU777229B2 (de)
CA (1) CA2351634C (de)
DE (2) DE69923180T2 (de)
ES (1) ES2269189T3 (de)
IL (1) IL143141A0 (de)
NO (1) NO20012561L (de)
NZ (1) NZ511759A (de)
RU (1) RU2260055C2 (de)
WO (1) WO2001023583A2 (de)

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EP1088891B1 (de) 1999-09-28 2005-01-12 Roche Diagnostics GmbH Thermostabiles Enzym welches die Genauigkeit thermostabiler DNA Polymerasen erhöht - zur Verbesserung der Nucleinsäuresynthese und in vitro Amplifikation
EP1275735A1 (de) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung
EP1277841B1 (de) * 2001-07-11 2009-08-26 Roche Diagnostics GmbH Neue Zusammensetzung und Methode zur 'hot start' Nukleinsäureamplifizierung
CA2409775C (en) 2001-12-03 2010-07-13 F. Hoffmann-La Roche Ag Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro
US7932070B2 (en) 2001-12-21 2011-04-26 Agilent Technologies, Inc. High fidelity DNA polymerase compositions and uses therefor
US20030180741A1 (en) * 2001-12-21 2003-09-25 Holly Hogrefe High fidelity DNA polymerase compositions and uses therefor
US9181534B1 (en) 2001-12-21 2015-11-10 Agilent Technologies Inc. High fidelity DNA polymerase compositions and uses thereof
GB0208768D0 (en) 2002-04-17 2002-05-29 Univ Newcastle DNA polymerases
US8283148B2 (en) 2002-10-25 2012-10-09 Agilent Technologies, Inc. DNA polymerase compositions for quantitative PCR and methods thereof
EP1502958A1 (de) * 2003-08-01 2005-02-02 Roche Diagnostics GmbH Neues Format zur Detektion von Heissstart- und Echtzeit-Polymerasekettenreaktion
WO2006061994A1 (ja) * 2004-12-08 2006-06-15 Takeshi Yamamoto 遺伝子配列検査法
US7922177B2 (en) * 2005-05-18 2011-04-12 Diamond Game Enterprises, Inc. Ticket strips that encourage multiple ticket purchasing
EP1960538A2 (de) * 2005-11-18 2008-08-27 Bioline Limited Verfahren zur verbesserung enzymatischer dna-polymerase-reaktionen
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
US8409805B2 (en) 2009-02-13 2013-04-02 Asuragen, Inc. Method of amplification of GC-rich DNA templates
US8679757B2 (en) * 2009-03-24 2014-03-25 Asuragen, Inc. PCR methods for characterizing the 5′ untranslated region of the FMR1 and FMR2 genes
BRPI1006682B1 (pt) * 2009-04-16 2020-04-22 Koninl Philips Electronics Nv método para a quantificação relativa de no mínimo um ácido nucleico alvo em uma amostra, método para a quantificação absoluta de no mínimo um ácido nucleico alvo em uma amostra, meios legíveis por máquina e equipamento para a análise das amostras de ácido nucleico
GB0915796D0 (en) * 2009-09-09 2009-10-07 Fermentas Uab Polymerase compositions and uses
US9238832B2 (en) * 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
EP2582806B1 (de) 2010-06-18 2015-12-16 Roche Diagnostics GmbH Dna-polymerasen mit erhöhter unterscheidung von 3'-diskrepanz
KR101622442B1 (ko) 2010-06-18 2016-05-19 에프. 호프만-라 로슈 아게 ３’―미스매치 구별이 증가된 ｄｎａ 중합효소
EP2582801B1 (de) 2010-06-18 2015-04-22 Roche Diagnostics GmbH Dna polymerasen mit erhöhter unterscheidung von 3' fehlpaarungen
JP5876478B2 (ja) 2010-06-18 2016-03-02 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ
US8741617B2 (en) 2010-06-18 2014-06-03 Roche Molecular Systems, Inc. DNA polymerases with increased 3′-mismatch discrimination
WO2011157436A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
US8759062B2 (en) 2010-06-18 2014-06-24 Roche Molecular Systems, Inc. DNA polymerases with increased 3′- mismatch discrimination
JP5926246B2 (ja) 2010-06-18 2016-05-25 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ
WO2012110061A1 (en) 2011-02-15 2012-08-23 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
CA2831180C (en) 2011-04-11 2017-02-14 Keith Bauer Dna polymerases with improved activity
EP2737061B1 (de) 2011-07-28 2015-09-16 Roche Diagnostics GmbH Dna-polymerasen mit verbesserter aktivität
EP2559774A1 (de) * 2011-08-17 2013-02-20 Roche Diagniostics GmbH Verbessertes Verfahren zur Amplifikation von Target-Nucleinsäuren mithilfe eines Multi-Primer-Ansatzes
CA2858264C (en) 2011-12-08 2018-01-02 F. Hoffmann-La Roche Ag Dna polymerases with improved activity
ES2716277T3 (es) 2011-12-08 2019-06-11 Hoffmann La Roche ADN polimerasas con actividad mejorada
CN103987844B (zh) 2011-12-08 2016-01-20 霍夫曼-拉罗奇有限公司 具有改进活性的dna聚合酶
ES2759023T3 (es) 2012-07-20 2020-05-07 Asuragen Inc Genotipificación completa de FMR1
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
GB2569965A (en) 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
EP3850086A1 (de) 2018-09-13 2021-07-21 F. Hoffmann-La Roche AG Mutante dna-polymerase(n) mit verbesserter strangverschiebbarkeit
WO2022071888A1 (en) * 2020-10-02 2022-04-07 National University Of Singapore A dna assembly mix and method of uses thereof

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WO1990011372A1 (en) * 1989-03-21 1990-10-04 Collaborative Research, Inc. Multiplex dna diagnostic test
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US6410277B1 (en) * 1993-02-19 2002-06-25 Takara Shuzo Co., Ltd. DNA polymersases with enhanced length of primer extension
AU6418294A (en) * 1993-03-30 1994-10-24 United States Biochemical Corporation Use of exonuclease in dna sequencing
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
CA2176193A1 (en) * 1995-05-22 1996-11-23 John Wesley Backus Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers
CN1552869A (zh) * 1995-12-27 2004-12-08 宝酒造株式会社 新型dna聚合酶
AU7683998A (en) * 1997-04-08 1998-10-30 Rockfeller University, The Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
US6238905B1 (en) * 1997-09-12 2001-05-29 University Technology Corporation Thermophilic polymerase III holoenzyme
DE19810879A1 (de) * 1998-03-13 1999-09-16 Roche Diagnostics Gmbh Polymerasenchimären
DE19813317A1 (de) * 1998-03-26 1999-09-30 Roche Diagnostics Gmbh Verbessertes Verfahren zur Primer Extension Präamplifikations-PCR
EP1175501A4 (de) * 1999-05-12 2002-11-27 Invitrogen Corp Zusammensetzungen und verfahren zur erhöhung der empfindlichkeit und spezifität der nukleinsäuresynthese
EP1088891B1 (de) * 1999-09-28 2005-01-12 Roche Diagnostics GmbH Thermostabiles Enzym welches die Genauigkeit thermostabiler DNA Polymerasen erhöht - zur Verbesserung der Nucleinsäuresynthese und in vitro Amplifikation
EP1275735A1 (de) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung

Also Published As

Publication number Publication date
JP3655240B2 (ja) 2005-06-02
IL143141A0 (en) 2002-04-21
CA2351634A1 (en) 2001-04-05
CN1343254A (zh) 2002-04-03
EP1088891A1 (de) 2001-04-04
US7030220B1 (en) 2006-04-18
JP2003510084A (ja) 2003-03-18
CN1185347C (zh) 2005-01-19
CA2351634C (en) 2007-02-20
NO20012561L (no) 2001-07-18
DE69923180T2 (de) 2006-01-12
ES2269189T3 (es) 2007-04-01
US7410782B2 (en) 2008-08-12
AU7907700A (en) 2001-04-30
EP1144653A2 (de) 2001-10-17
NZ511759A (en) 2003-12-19
NO20012561D0 (no) 2001-05-25
RU2260055C2 (ru) 2005-09-10
DE69923180D1 (de) 2005-02-17
AU777229B2 (en) 2004-10-07
US20060078928A1 (en) 2006-04-13
EP1144653B1 (de) 2006-08-09
ATE286981T1 (de) 2005-01-15
EP1088891B1 (de) 2005-01-12
DE60029927T2 (de) 2007-03-08
ATE335825T1 (de) 2006-09-15
WO2001023583A2 (en) 2001-04-05
WO2001023583A3 (en) 2001-10-25

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