DE60020146D1 - Two-hybrid-Screeningverfahren unter Verwendung des bakteriellen Transcriptionsaktivators sigma-54 - Google Patents
Two-hybrid-Screeningverfahren unter Verwendung des bakteriellen Transcriptionsaktivators sigma-54Info
- Publication number
- DE60020146D1 DE60020146D1 DE60020146T DE60020146T DE60020146D1 DE 60020146 D1 DE60020146 D1 DE 60020146D1 DE 60020146 T DE60020146 T DE 60020146T DE 60020146 T DE60020146 T DE 60020146T DE 60020146 D1 DE60020146 D1 DE 60020146D1
- Authority
- DE
- Germany
- Prior art keywords
- sigma
- screening method
- transcriptional activator
- hybrid screening
- bacterial transcriptional
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000001580 bacterial effect Effects 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 108091006106 transcriptional activators Proteins 0.000 title 1
- 238000010396 two-hybrid screening Methods 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 101100273253 Rhizopus niveus RNAP gene Proteins 0.000 abstract 1
- 108091006088 activator proteins Proteins 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000012248 genetic selection Methods 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Circuits Of Receivers In General (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Vehicle Body Suspensions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9921155 | 1999-09-08 | ||
| GBGB9921155.9A GB9921155D0 (en) | 1999-09-08 | 1999-09-08 | Selection system |
| PCT/GB2000/003450 WO2001018244A2 (en) | 1999-09-08 | 2000-09-08 | Selection system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60020146D1 true DE60020146D1 (de) | 2005-06-16 |
| DE60020146T2 DE60020146T2 (de) | 2005-10-06 |
Family
ID=10860529
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60020146T Expired - Fee Related DE60020146T2 (de) | 1999-09-08 | 2000-09-08 | Two-hybrid-Screeningverfahren unter Verwendung des bakteriellen Transcriptionsaktivators sigma-54 |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP1224324B1 (de) |
| AT (1) | ATE295429T1 (de) |
| AU (1) | AU7025100A (de) |
| DE (1) | DE60020146T2 (de) |
| GB (1) | GB9921155D0 (de) |
| WO (1) | WO2001018244A2 (de) |
Families Citing this family (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK1019496T3 (da) | 1997-07-07 | 2005-01-10 | Medical Res Council | In vitro-sorteringsmetode |
| GB0127564D0 (en) | 2001-11-16 | 2002-01-09 | Medical Res Council | Emulsion compositions |
| EP1594950A4 (de) | 2003-01-29 | 2007-07-25 | 454 Corp | Verfahren zur herstellung von einzelstrang-dna-bibliotheken |
| US7575865B2 (en) | 2003-01-29 | 2009-08-18 | 454 Life Sciences Corporation | Methods of amplifying and sequencing nucleic acids |
| GB0307403D0 (en) | 2003-03-31 | 2003-05-07 | Medical Res Council | Selection by compartmentalised screening |
| US20060078893A1 (en) | 2004-10-12 | 2006-04-13 | Medical Research Council | Compartmentalised combinatorial chemistry by microfluidic control |
| GB0307428D0 (en) | 2003-03-31 | 2003-05-07 | Medical Res Council | Compartmentalised combinatorial chemistry |
| CA2531105C (en) | 2003-07-05 | 2015-03-17 | The Johns Hopkins University | Method and compositions for detection and enumeration of genetic variations |
| WO2005073410A2 (en) | 2004-01-28 | 2005-08-11 | 454 Corporation | Nucleic acid amplification with continuous flow emulsion |
| US20050221339A1 (en) | 2004-03-31 | 2005-10-06 | Medical Research Council Harvard University | Compartmentalised screening by microfluidic control |
| US7968287B2 (en) | 2004-10-08 | 2011-06-28 | Medical Research Council Harvard University | In vitro evolution in microfluidic systems |
| JP4564926B2 (ja) | 2005-01-25 | 2010-10-20 | エワ ユニバーシティ−インダストリー コラボレーション ファウンデーション | ヒスタミン分泌能を有する欠失型ＩｇＥ依存的ヒスタミン放出因子、ＨＲＦ結合ペプチドおよびその利用方法 |
| EP1984738A2 (de) | 2006-01-11 | 2008-10-29 | Raindance Technologies, Inc. | Mikrofluidische vorrichtungen und verfahren zur verwendung bei der bildung und kontrolle von nanoreaktoren |
| US9562837B2 (en) | 2006-05-11 | 2017-02-07 | Raindance Technologies, Inc. | Systems for handling microfludic droplets |
| WO2007133710A2 (en) | 2006-05-11 | 2007-11-22 | Raindance Technologies, Inc. | Microfluidic devices and methods of use thereof |
| EP2077912B1 (de) | 2006-08-07 | 2019-03-27 | The President and Fellows of Harvard College | Fluorkohlenstoffemulsionsstabilisierende tenside |
| US8772046B2 (en) | 2007-02-06 | 2014-07-08 | Brandeis University | Manipulation of fluids and reactions in microfluidic systems |
| WO2008130623A1 (en) | 2007-04-19 | 2008-10-30 | Brandeis University | Manipulation of fluids, fluid components and reactions in microfluidic systems |
| WO2010009365A1 (en) | 2008-07-18 | 2010-01-21 | Raindance Technologies, Inc. | Droplet libraries |
| US12038438B2 (en) | 2008-07-18 | 2024-07-16 | Bio-Rad Laboratories, Inc. | Enzyme quantification |
| US8528589B2 (en) | 2009-03-23 | 2013-09-10 | Raindance Technologies, Inc. | Manipulation of microfluidic droplets |
| EP2486409A1 (de) | 2009-10-09 | 2012-08-15 | Universite De Strasbourg | Markiertes nanomaterial auf siliziumbasis mit verbesserten eigenschaften und seine verwendung |
| WO2011079176A2 (en) | 2009-12-23 | 2011-06-30 | Raindance Technologies, Inc. | Microfluidic systems and methods for reducing the exchange of molecules between droplets |
| EP4484577A3 (de) | 2010-02-12 | 2025-03-26 | Bio-Rad Laboratories, Inc. | Digitale analytanalyse |
| US9366632B2 (en) | 2010-02-12 | 2016-06-14 | Raindance Technologies, Inc. | Digital analyte analysis |
| US9399797B2 (en) | 2010-02-12 | 2016-07-26 | Raindance Technologies, Inc. | Digital analyte analysis |
| US10351905B2 (en) | 2010-02-12 | 2019-07-16 | Bio-Rad Laboratories, Inc. | Digital analyte analysis |
| WO2012045012A2 (en) | 2010-09-30 | 2012-04-05 | Raindance Technologies, Inc. | Sandwich assays in droplets |
| WO2012109600A2 (en) | 2011-02-11 | 2012-08-16 | Raindance Technologies, Inc. | Methods for forming mixed droplets |
| US9150852B2 (en) | 2011-02-18 | 2015-10-06 | Raindance Technologies, Inc. | Compositions and methods for molecular labeling |
| EP3709018A1 (de) | 2011-06-02 | 2020-09-16 | Bio-Rad Laboratories, Inc. | Mikrofluidische vorrichtung zum nachweis von komponenten einer chemischen reaktion |
| US8841071B2 (en) | 2011-06-02 | 2014-09-23 | Raindance Technologies, Inc. | Sample multiplexing |
| US8658430B2 (en) | 2011-07-20 | 2014-02-25 | Raindance Technologies, Inc. | Manipulating droplet size |
| US11901041B2 (en) | 2013-10-04 | 2024-02-13 | Bio-Rad Laboratories, Inc. | Digital analysis of nucleic acid modification |
| US9944977B2 (en) | 2013-12-12 | 2018-04-17 | Raindance Technologies, Inc. | Distinguishing rare variations in a nucleic acid sequence from a sample |
| WO2015103367A1 (en) | 2013-12-31 | 2015-07-09 | Raindance Technologies, Inc. | System and method for detection of rna species |
| US10647981B1 (en) | 2015-09-08 | 2020-05-12 | Bio-Rad Laboratories, Inc. | Nucleic acid library generation methods and compositions |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PT963376E (pt) * | 1996-12-11 | 2005-06-30 | Bristol Myers Squibb Co | Sistema procariotico duplamente hibrido |
| US6333154B1 (en) * | 1997-12-04 | 2001-12-25 | Institut Pasteur | Bacterial multi-hybrid system and applications thereof |
-
1999
- 1999-09-08 GB GBGB9921155.9A patent/GB9921155D0/en not_active Ceased
-
2000
- 2000-09-08 WO PCT/GB2000/003450 patent/WO2001018244A2/en not_active Ceased
- 2000-09-08 DE DE60020146T patent/DE60020146T2/de not_active Expired - Fee Related
- 2000-09-08 AU AU70251/00A patent/AU7025100A/en not_active Abandoned
- 2000-09-08 EP EP00958835A patent/EP1224324B1/de not_active Expired - Lifetime
- 2000-09-08 AT AT00958835T patent/ATE295429T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| EP1224324A2 (de) | 2002-07-24 |
| WO2001018244A3 (en) | 2001-11-15 |
| DE60020146T2 (de) | 2005-10-06 |
| AU7025100A (en) | 2001-04-10 |
| GB9921155D0 (en) | 1999-11-10 |
| ATE295429T1 (de) | 2005-05-15 |
| WO2001018244A2 (en) | 2001-03-15 |
| EP1224324B1 (de) | 2005-05-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |