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DE502007005530D1 - Microorganism strain for the production of recombinant proteins - Google Patents

Microorganism strain for the production of recombinant proteins

Info

Publication number
DE502007005530D1
DE502007005530D1 DE502007005530T DE502007005530T DE502007005530D1 DE 502007005530 D1 DE502007005530 D1 DE 502007005530D1 DE 502007005530 T DE502007005530 T DE 502007005530T DE 502007005530 T DE502007005530 T DE 502007005530T DE 502007005530 D1 DE502007005530 D1 DE 502007005530D1
Authority
DE
Germany
Prior art keywords
host
microbial strain
recombinant protein
medium
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE502007005530T
Other languages
German (de)
Inventor
Susanne Leonhartsberger
Guenter Wich
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wacker Chemie AG
Original Assignee
Wacker Chemie AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wacker Chemie AG filed Critical Wacker Chemie AG
Publication of DE502007005530D1 publication Critical patent/DE502007005530D1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

New microbial strain comprises a gene coding for a recombinant protein that is secreted during fermentation and a mutated gene that codes for a host protein and has a mutation such that expression of the host protein is reduced. Independent claims are also included for: (1) producing a microbial strain as above by transforming a host cells with the gene for the recombinant protein, culturing the cell in a fermentation medium so that the recombinant protein and host proteins are secreted into the medium, characterizing the host proteins, and reducing or preventing expression of a gene coding for one of the host proteins; (2) producing a recombinant protein by culturing a microbial strain as above in a fermentation medium and then separating the medium from the cells of the microbial strain.
DE502007005530T 2006-02-02 2007-01-29 Microorganism strain for the production of recombinant proteins Active DE502007005530D1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102006004871A DE102006004871A1 (en) 2006-02-02 2006-02-02 Microbial strain useful for producing recombinant proteins comprises a gene coding for a recombinant protein and a gene that codes for a host protein and is mutated to reduce expression of the host protein

Publications (1)

Publication Number Publication Date
DE502007005530D1 true DE502007005530D1 (en) 2010-12-16

Family

ID=38282098

Family Applications (2)

Application Number Title Priority Date Filing Date
DE102006004871A Ceased DE102006004871A1 (en) 2006-02-02 2006-02-02 Microbial strain useful for producing recombinant proteins comprises a gene coding for a recombinant protein and a gene that codes for a host protein and is mutated to reduce expression of the host protein
DE502007005530T Active DE502007005530D1 (en) 2006-02-02 2007-01-29 Microorganism strain for the production of recombinant proteins

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE102006004871A Ceased DE102006004871A1 (en) 2006-02-02 2006-02-02 Microbial strain useful for producing recombinant proteins comprises a gene coding for a recombinant protein and a gene that codes for a host protein and is mutated to reduce expression of the host protein

Country Status (6)

Country Link
US (1) US7749756B2 (en)
EP (3) EP2418276B1 (en)
JP (1) JP4751347B2 (en)
AT (1) ATE486935T1 (en)
DE (2) DE102006004871A1 (en)
DK (2) DK2418276T3 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8060609B2 (en) 2008-01-04 2011-11-15 Sling Media Inc. Systems and methods for determining attributes of media items accessed via a personal media broadcaster
US8327407B2 (en) 2009-10-27 2012-12-04 Sling Media, Inc. Determination of receiving live versus time-shifted media content at a communication device
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine
WO2025252302A1 (en) 2024-06-05 2025-12-11 Wacker Chemie Ag Plasmids with an increased replication rate

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3813107A1 (en) * 1988-04-19 1989-11-02 Consortium Elektrochem Ind SECRETOR MUTANT OF ESCHERICHIA COLI
DE4009268A1 (en) 1990-03-22 1991-09-26 Consortium Elektrochem Ind SECRETION OF HIRUDIN DERIVATIVES
US5264365A (en) * 1990-11-09 1993-11-23 Board Of Regents, The University Of Texas System Protease-deficient bacterial strains for production of proteolytically sensitive polypeptides
CA2116774C (en) * 1991-09-19 2003-11-11 Paul J. Carter Expression in e. coli antibody fragments having at least a cysteine present as a free thiol. use for the production of bifunctional f(ab') 2 antibodies
SE9203753D0 (en) 1992-12-11 1992-12-11 Kabi Pharmacia Ab EXPRESSION SYSTEM FOR PRODUCING APOLIPOPROTEIN AI-M
JP3090853B2 (en) 1994-08-19 2000-09-25 新日本製鐵株式会社 Manufacturing method of semiconductor device with bump
US5789199A (en) * 1994-11-03 1998-08-04 Genentech, Inc. Process for bacterial production of polypeptides
US5830692A (en) * 1995-03-24 1998-11-03 Consortium Fur Elektrochemische Industrie Gmbh Expression system which can be regulated
GB9828711D0 (en) * 1998-12-24 1999-02-17 Genencor Int Production of proteins in gram-positive microorganisms
EP1572888A2 (en) * 2001-09-28 2005-09-14 Selectx Pharmaceuticals, Inc. Methods for generating, selecting, and identifying compounds which bind a target molecule
US6989265B2 (en) * 2002-01-23 2006-01-24 Wisconsin Alumni Research Foundation Bacteria with reduced genome
EP2341070A3 (en) * 2002-03-29 2011-10-19 Genencor International, Inc. Ehanced protein expression in bacillus
US7195757B2 (en) * 2002-04-15 2007-03-27 Washington University Modulation of immune responses to foreign antigens expressed by recombinant attenuated bacterial vectors
GB0224082D0 (en) 2002-10-16 2002-11-27 Celltech R&D Ltd Biological products
KR100570422B1 (en) * 2003-10-16 2006-04-11 한미약품 주식회사 Expression vectors for secreting and producing antibody fragments using E. coli secretion sequences and methods for mass production of antibody fragments using the same
US20050106703A1 (en) * 2003-11-05 2005-05-19 Kyowa Hakko Kogyo Co., Ltd. Microorganisms producing dipeptides and process for producing dipeptides using the microorganisms
KR100656529B1 (en) * 2004-04-08 2006-12-13 한국원자력연구소 Mass production method of recombinant E. coli and polyhydroxybutyrate using E. coli
JP2007536274A (en) * 2004-05-07 2007-12-13 ザ、リージェンツ、オブ、ザ、ユニバーシティ、オブ、カリフォルニア Endothelial cell apoptosis induced by fibrinogen γ chain C-terminal fragment
DK1903105T3 (en) 2006-09-22 2010-06-28 Wacker Chemie Ag Process for Fermentative Protein Production

Also Published As

Publication number Publication date
EP1897939A2 (en) 2008-03-12
EP2418276B1 (en) 2013-11-20
EP2418276A1 (en) 2012-02-15
EP2298912A3 (en) 2011-09-28
US20080064062A1 (en) 2008-03-13
DK2418276T3 (en) 2014-02-24
DE102006004871A1 (en) 2007-08-09
EP1897939B1 (en) 2010-11-03
ATE486935T1 (en) 2010-11-15
EP2298912A2 (en) 2011-03-23
JP2007202559A (en) 2007-08-16
JP4751347B2 (en) 2011-08-17
US7749756B2 (en) 2010-07-06
DK1897939T3 (en) 2011-01-31
EP1897939A3 (en) 2008-03-19

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