DE4338119A1 - Spezifische Gensonden und Verfahren zum quantitativen Nachweis von methicillinresistenten Staphylococcen - Google Patents

Spezifische Gensonden und Verfahren zum quantitativen Nachweis von methicillinresistenten Staphylococcen

Info

Publication number: DE4338119A1
Authority: DE; Germany
Prior art keywords: methicillin; dna; amplification; gene; resistant
Prior art date: 1993-11-08
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Withdrawn

Application number

DE4338119A

Other languages

German (de)

English (en)

Inventor

Wolfgang Dr Springer

Rainer Dr Endermann

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Bayer AG

Original Assignee

Bayer AG

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1993-11-08

Filing date

1993-11-08

Publication date

1995-05-11

1993-11-08 Application filed by Bayer AG filed Critical Bayer AG

1993-11-08 Priority to DE4338119A priority Critical patent/DE4338119A1/de

1994-10-28 Priority to PCT/EP1994/003553 priority patent/WO1995013395A1/fr

1995-05-11 Publication of DE4338119A1 publication Critical patent/DE4338119A1/de

Status Withdrawn legal-status Critical Current

Links

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229960000274 lysozyme Drugs 0.000 description 1
239000004325 lysozyme Substances 0.000 description 1
235000010335 lysozyme Nutrition 0.000 description 1
239000003120 macrolide antibiotic agent Substances 0.000 description 1
229940041033 macrolides Drugs 0.000 description 1
238000002844 melting Methods 0.000 description 1
230000008018 melting Effects 0.000 description 1
238000002156 mixing Methods 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
JPXMTWWFLBLUCD-UHFFFAOYSA-N nitro blue tetrazolium(2+) Chemical compound COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 JPXMTWWFLBLUCD-UHFFFAOYSA-N 0.000 description 1
229920001220 nitrocellulos Polymers 0.000 description 1
239000003960 organic solvent Substances 0.000 description 1
HHXMXAQDOUCLDN-RXMQYKEDSA-N penem Chemical compound S1C=CN2C(=O)C[C@H]21 HHXMXAQDOUCLDN-RXMQYKEDSA-N 0.000 description 1
150000002960 penicillins Chemical class 0.000 description 1
JTJMJGYZQZDUJJ-UHFFFAOYSA-N phencyclidine Chemical class C1CCCCN1C1(C=2C=CC=CC=2)CCCCC1 JTJMJGYZQZDUJJ-UHFFFAOYSA-N 0.000 description 1
230000004260 plant-type cell wall biogenesis Effects 0.000 description 1
238000003752 polymerase chain reaction Methods 0.000 description 1
239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
239000003910 polypeptide antibiotic agent Substances 0.000 description 1
229920000136 polysorbate Polymers 0.000 description 1
229910052700 potassium Inorganic materials 0.000 description 1
239000011591 potassium Substances 0.000 description 1
238000011002 quantification Methods 0.000 description 1
238000011158 quantitative evaluation Methods 0.000 description 1
150000007660 quinolones Chemical class 0.000 description 1
238000000163 radioactive labelling Methods 0.000 description 1
239000011535 reaction buffer Substances 0.000 description 1
238000011160 research Methods 0.000 description 1
108091008146 restriction endonucleases Proteins 0.000 description 1
JQXXHWHPUNPDRT-WLSIYKJHSA-N rifampicin Chemical compound O([C@](C1=O)(C)O/C=C/[C@@H]([C@H]([C@@H](OC(C)=O)[C@H](C)[C@H](O)[C@H](C)[C@@H](O)[C@@H](C)\C=C\C=C(C)/C(=O)NC=2C(O)=C3C([O-])=C4C)C)OC)C4=C1C3=C(O)C=2\C=N\N1CC[NH+](C)CC1 JQXXHWHPUNPDRT-WLSIYKJHSA-N 0.000 description 1
229960001225 rifampicin Drugs 0.000 description 1
239000013049 sediment Substances 0.000 description 1
238000013207 serial dilution Methods 0.000 description 1
239000007790 solid phase Substances 0.000 description 1
239000005720 sucrose Substances 0.000 description 1
229960001608 teicoplanin Drugs 0.000 description 1
238000010998 test method Methods 0.000 description 1
238000002560 therapeutic procedure Methods 0.000 description 1
230000009466 transformation Effects 0.000 description 1
238000013519 translation Methods 0.000 description 1
MYPYJXKWCTUITO-LYRMYLQWSA-N vancomycin Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C2C=C3C=C1OC1=CC=C(C=C1Cl)[C@@H](O)[C@H](C(N[C@@H](CC(N)=O)C(=O)N[C@H]3C(=O)N[C@H]1C(=O)N[C@H](C(N[C@@H](C3=CC(O)=CC(O)=C3C=3C(O)=CC=C1C=3)C(O)=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)O2)=O)NC(=O)[C@@H](CC(C)C)NC)[C@H]1C[C@](C)(N)[C@H](O)[C@H](C)O1 MYPYJXKWCTUITO-LYRMYLQWSA-N 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
150000003952 β-lactams Chemical class 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

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Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Analytical Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Immunology (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

DE4338119A 1993-11-08 1993-11-08 Spezifische Gensonden und Verfahren zum quantitativen Nachweis von methicillinresistenten Staphylococcen Withdrawn DE4338119A1 (de)

Priority Applications (2)

Application Number	Priority Date	Filing Date	Title
DE4338119A DE4338119A1 (de)	1993-11-08	1993-11-08	Spezifische Gensonden und Verfahren zum quantitativen Nachweis von methicillinresistenten Staphylococcen
PCT/EP1994/003553 WO1995013395A1 (fr)	1993-11-08	1994-10-28	Analyseurs specifiques de genes et procedes de determination quantitative de staphylocoques resistant a la methicilline

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DE4338119A DE4338119A1 (de)	1993-11-08	1993-11-08	Spezifische Gensonden und Verfahren zum quantitativen Nachweis von methicillinresistenten Staphylococcen

Publications (1)

Publication Number	Publication Date
DE4338119A1 true DE4338119A1 (de)	1995-05-11

Family

ID=6502091

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DE4338119A Withdrawn DE4338119A1 (de)	1993-11-08	1993-11-08	Spezifische Gensonden und Verfahren zum quantitativen Nachweis von methicillinresistenten Staphylococcen

Country Status (2)

Country	Link
DE (1)	DE4338119A1 (fr)
WO (1)	WO1995013395A1 (fr)

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EP0688873A3 (fr) *	1994-06-23	1999-02-24	Bayer Ag	Test DNA rapide pour la détection de staphylococcus aureus résistant à la chinolone dans du matériel clinique
WO1999001572A3 (fr) *	1997-07-03	1999-03-25	Id Biomedical Corp	Procedes permettant de detecter rapidement les staphyloccoques resistant a la methicilline
EP0887424A4 (fr) *	1996-02-23	2003-05-02	Kainos Lab Inc	Procede de diagnostic

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US7297517B2 (en)	2000-05-29	2007-11-20	Tosoh Corporation	Oligonucleotides and method for detection of meca gene of methicillin-resistant Staphylococcus aureus
US7666588B2 (en)	2001-03-02	2010-02-23	Ibis Biosciences, Inc.	Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
US20040121313A1 (en)	2002-12-06	2004-06-24	Ecker David J.	Methods for rapid detection and identification of bioagents in organs for transplantation
US20030027135A1 (en)	2001-03-02	2003-02-06	Ecker David J.	Method for rapid detection and identification of bioagents
US7226739B2 (en)	2001-03-02	2007-06-05	Isis Pharmaceuticals, Inc	Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations
CA2348042A1 (fr)	2001-06-04	2002-12-04	Ann Huletsky	Sequences permettant de detecter et d'identifier des staphylococcus aureus resistant a la meticilline
US7217510B2 (en)	2001-06-26	2007-05-15	Isis Pharmaceuticals, Inc.	Methods for providing bacterial bioagent characterizing information
US8073627B2 (en)	2001-06-26	2011-12-06	Ibis Biosciences, Inc.	System for indentification of pathogens
JP2006516193A (ja)	2002-12-06	2006-06-29	アイシス・ファーマシューティカルス・インコーポレーテッド	ヒトおよび動物における病原体の迅速な同定方法
US8057993B2 (en)	2003-04-26	2011-11-15	Ibis Biosciences, Inc.	Methods for identification of coronaviruses
US7964343B2 (en)	2003-05-13	2011-06-21	Ibis Biosciences, Inc.	Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US8158354B2 (en)	2003-05-13	2012-04-17	Ibis Biosciences, Inc.	Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US8097416B2 (en)	2003-09-11	2012-01-17	Ibis Biosciences, Inc.	Methods for identification of sepsis-causing bacteria
US8394945B2 (en)	2003-09-11	2013-03-12	Ibis Biosciences, Inc.	Compositions for use in identification of bacteria
US8546082B2 (en)	2003-09-11	2013-10-01	Ibis Biosciences, Inc.	Methods for identification of sepsis-causing bacteria
US7666592B2 (en)	2004-02-18	2010-02-23	Ibis Biosciences, Inc.	Methods for concurrent identification and quantification of an unknown bioagent
ES2641832T3 (es)	2004-05-24	2017-11-14	Ibis Biosciences, Inc.	Espectrometría de masas con filtración de iones selectiva por establecimiento de umbrales digitales
US20050266411A1 (en)	2004-05-25	2005-12-01	Hofstadler Steven A	Methods for rapid forensic analysis of mitochondrial DNA
US7811753B2 (en)	2004-07-14	2010-10-12	Ibis Biosciences, Inc.	Methods for repairing degraded DNA
WO2006135400A2 (fr)	2004-08-24	2006-12-21	Isis Pharmaceuticals, Inc.	Procedes pour l'identification rapide d'organismes recombinants
US8084207B2 (en)	2005-03-03	2011-12-27	Ibis Bioscience, Inc.	Compositions for use in identification of papillomavirus
US20060205040A1 (en)	2005-03-03	2006-09-14	Rangarajan Sampath	Compositions for use in identification of adventitious viruses
US20060210998A1 (en) *	2005-03-18	2006-09-21	Christiane Kettlitz	Determination of antibiotic resistance in staphylococcus aureus
WO2007014045A2 (fr)	2005-07-21	2007-02-01	Isis Pharmaceuticals, Inc.	Procede pour l'identification et la quantification rapide de variants d'acide nucleique
US7838221B2 (en)	2005-10-11	2010-11-23	Geneohm Sciences, Inc.	Sequences for detection and identification of methicillin-resistant Staphylococcus aureus (MRSA)
US11834720B2 (en)	2005-10-11	2023-12-05	Geneohm Sciences, Inc.	Sequences for detection and identification of methicillin-resistant Staphylococcus aureus (MRSA) of MREJ types xi to xx
EP2064332B1 (fr)	2006-09-14	2012-07-18	Ibis Biosciences, Inc.	Procédé d'amplification ciblée de génome entier pour l'identification d'agents pathogènes
DK2118311T3 (da)	2006-12-19	2013-07-15	Geneohm Sciences Inc	Påvisning af staphylococcus aureus og identificering af methicillinresistent staphylococcus aureus
US8871471B2 (en)	2007-02-23	2014-10-28	Ibis Biosciences, Inc.	Methods for rapid forensic DNA analysis
US9598724B2 (en)	2007-06-01	2017-03-21	Ibis Biosciences, Inc.	Methods and compositions for multiple displacement amplification of nucleic acids
WO2010033599A2 (fr)	2008-09-16	2010-03-25	Ibis Biosciences, Inc.	Cartouches de mélange, postes de mélange et kits, systèmes et procédés associés
US8148163B2 (en)	2008-09-16	2012-04-03	Ibis Biosciences, Inc.	Sample processing units, systems, and related methods
EP2344893B1 (fr)	2008-09-16	2014-10-15	Ibis Biosciences, Inc.	Systèmes et procédés de manipulation de microplaques
US8158936B2 (en)	2009-02-12	2012-04-17	Ibis Biosciences, Inc.	Ionization probe assemblies
WO2011008972A1 (fr)	2009-07-17	2011-01-20	Ibis Biosciences, Inc.	Systèmes pour l'identification d'un bioagent
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WO1992005281A1 (fr) *	1990-09-14	1992-04-02	Chugai Seiyaku Kabushiki Kaisha	Procede de detection de staphylococcus aureus resistant au mesitylene-cephem
JPH04169200A (ja) *	1990-10-31	1992-06-17	Shionogi & Co Ltd	Ｐｂｐ２’遺伝子の検出方法およびメチシリン耐性判定法
CA2063316A1 (fr) *	1991-03-19	1992-09-20	Larry C. Blaszczak	Sequence d'adn et d'acides amines de la proteine 2a fixant la penicilline de la souche 27r de staphylococcus aureus et derives utilises pour leur purification et dosage pour
JPH0549477A (ja) *	1991-08-05	1993-03-02	Wakunaga Pharmaceut Co Ltd	スタフイロコツカス属細菌類の検出
CA2075423A1 (fr) *	1991-08-13	1993-02-14	Paul Luther Skatrud	Methode de detection rapide des staphylocoques resistant a la methicilline

1993
- 1993-11-08 DE DE4338119A patent/DE4338119A1/de not_active Withdrawn
1994
- 1994-10-28 WO PCT/EP1994/003553 patent/WO1995013395A1/fr not_active Ceased

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP0688873A3 (fr) *	1994-06-23	1999-02-24	Bayer Ag	Test DNA rapide pour la détection de staphylococcus aureus résistant à la chinolone dans du matériel clinique
US6015666A (en) *	1994-06-23	2000-01-18	Bayer Aktiengesellschaft	Rapid DNA test for detecting quinolone-resistant Staphylococcus aureus pathogens in clinical material
EP0887424A4 (fr) *	1996-02-23	2003-05-02	Kainos Lab Inc	Procede de diagnostic
WO1999001572A3 (fr) *	1997-07-03	1999-03-25	Id Biomedical Corp	Procedes permettant de detecter rapidement les staphyloccoques resistant a la methicilline
US6503709B1 (en)	1997-07-03	2003-01-07	Id Biomedical Corporation	Methods for rapidly detecting methicillin resistant staphylococci

Also Published As

Publication number	Publication date
WO1995013395A1 (fr)	1995-05-18

Publication	Publication Date	Title
DE4338119A1 (de)	1995-05-11	Spezifische Gensonden und Verfahren zum quantitativen Nachweis von methicillinresistenten Staphylococcen
US6015666A (en)	2000-01-18	Rapid DNA test for detecting quinolone-resistant Staphylococcus aureus pathogens in clinical material
JP4176146B2 (ja)	2008-11-05	微生物検査室における日常的診断用の臨床検体からの通常の細菌病原体および抗生物質耐性遺伝子を迅速に検出および同定するための特異的および普遍的プローブおよび増幅プライマー
US5726021A (en)	1998-03-10	Rapid and sensitive detection of antibiotic-resistant mycobacteria using oligonucleotide probes specific for ribosomal RNA precursors
EP1053352B1 (fr)	2002-09-18	Procedes de detection d'un mauvais appariement
JP2798499B2 (ja)	1998-09-17	感染症診断用プローブ
US5106730A (en)	1992-04-21	Lactam-containing compositions and methods useful for the hybridization of nucleic acids
JP2002509436A (ja)	2002-03-26	メチシリン耐性ブドウ球菌を迅速に検出するための方法
EP2844762B1 (fr)	2017-12-20	Test de sensibilité aux antibiotiques utilisant des sondes pour de l'arn pré-ribosomique
JPH11503921A (ja)	1999-04-06	種の同定のための普遍的標的
WO1991008305A1 (fr)	1991-06-13	Fragments d'adn et sondes et amorces d'adn a base de ceux-ci
EP0595167B1 (fr)	1998-01-07	Sonde génétique spécifique et méthode de diagnostic de Candia albicans
JP2558420B2 (ja)	1996-11-27	感染症診断用プローブ
US5968739A (en)	1999-10-19	Nucleic acid primers and probes for detecting Legionella pneumophila
WO1991002088A1 (fr)	1991-02-21	Compositions renfermant des lactames et procedes utiles pour l'hybridation d'acides nucleiques
Satokari et al.	2005	Multiplexed quantification of bacterial 16S rRNA by solution hybridization with oligonucleotide probes and affinity capture
EP0484385A1 (fr)	1992-05-13	Quantification de bacteries au moyen d'un dosage d'hybridation d'acide nucleique
JPH08297A (ja)	1996-01-09	バクテリアを検出するためのオリゴヌクレオチドプライマーおよびプローブ
JP2004201636A (ja)	2004-07-22	病原真菌遺伝子の検出方法及び真菌の菌種同定用オリゴヌクレオチド
EP1390541A2 (fr)	2004-02-25	Procede de detection de bacteries gram positif
JPH0588A (ja)	1993-01-08	新規核酸断片およびこれらを用いたマイコプラズマの検出法
JP2004201637A (ja)	2004-07-22	病原真菌遺伝子の検出及びそれに用いるオリゴヌクレオチド
Britschgi et al.	1995	Detection of rifampin-resistant bacteria using DNA probes for precursor rRNA
JP3652387B2 (ja)	2005-05-25	ペニシリン耐性肺炎球菌遺伝子断片及びその利用法
AU705198C (en)	2000-01-06	Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories

Legal Events

Date	Code	Title	Description
1997-11-06	8139	Disposal/non-payment of the annual fee