CZ37205U1 - Disinfectant - Google Patents

Description

Disinfectant

Field of technology

This technical solution refers to a disinfectant for surfaces and areas (form - concentrate), containing a quaternary ammonium compound (KAS), with a broad-spectrum effect against microbes, viruses, as well as fungi and fungi (bactericidal, virucidal and fungicidal), and then acting as an insecticide (against insects) and at the same time acting as a detergent (cleaning agent) according to the official opinion of the European Agency ECHA (page 2).

Current state of the art

Didecyldimethylammonium chloride is a quaternary ammonium compound with the chemical formula (CioH ₂ i) ₂ (CH ₃ ) ₂ NCl, used as an antiseptic, disinfectant, fungicide, emulsifier, catalyst and antistatic agent (detergent), see page 2 - ECHA.

Resistance to various bacteria, viruses and fungi is currently a very big problem in the field of disinfectants. Because it is possible to generally talk about newly emerging very strong (resistant) strains of bacteria, at the same time also about a relatively significant amount of resistant viruses and very resistant fungi (fungi release their spores into the air and they get into the respiratory tract).

One of the most widely used surfaces and surfaces disinfection is alcohol disinfection, or chlorine-based disinfection - a disadvantage, e.g. the smell of chlorine. At the same time, a major disadvantage of chlorine is its inability to effectively penetrate the biofilm (microorganisms forming the so-called microbial biofilm and thus are more resistant to toxic substances). Chlorine contained in disinfectants can also cause itching of the skin, burning in the eyes.

As far as alcohol disinfectants are concerned, the disadvantage is particularly strong flammability (explosiveness), drying of the skin and allergic manifestations (for hand disinfection), based on the frequent use of this disinfectant, as well as the aggressiveness of alcohol on various materials - surfaces.

Currently, disinfectants based on quaternary ammonium compounds (KAS) are gradually being promoted, due to their antibacterial, virucidal and fungicidal effects - DDAC shows a wide spectrum of effectiveness against gram-positive and gram-negative bacteria as well as fungicidal activity (phytopathogenic - fungi, bacteria and algae).

DDAC is a cationic surfactant containing 50% of the active quaternary ammonium compound Didecyl-dimethylammonium chloride, which is used as a biocide, fungicide, algicide and also as an insecticide (or acaracid), see article 1, page 2 - ECHA.

As already mentioned, the surface-active substance shows a wide spectrum of effectiveness against a number of gram-positive and gram-negative bacteria, as well as enveloped bacteria, as well as viruses - such as hepatitis B and HIV.

Didecyldimethylammonium chloride is also an insecticide for use also in biocidal products (insecticides, acaricides and preparations to control other arthropods), see article 2 - ECHA page 2.

Quaternary ammonium compounds are widely used in this area, e.g. as disinfectants with very good effectiveness against resistant strains of viruses, bacteria and at the same time also fungi, and also as - molluscocides and acaricides.

Didecyldimethylammonium chloride (DDAC) is a quaternary ammonium compound used

- 1 CZ 37205 U1 as an antiseptic disinfectant that causes disruption of intermolecular interactions and dissociation of lipid bilayers.

Bacteriostatic activity (prevents growth) or bactericidal activity (kills microorganisms), depending on the concentration of DDAC and simultaneously on the growth phase of the microbial population.

It is a broad-spectrum biocidal agent (see ECHA article p. 2) against bacteria, viruses and fungi and can be used as a disinfectant also for cleaning laundry, recommended for use in hospitals, hotels and industries. It is also used in gynecology, surgery, ophthalmology, paediatrics, OT and for the sterilization of surgical instruments, endoscopes and, last but not least, as surface disinfection.

Quaternary ammonium compounds are widely used, e.g. as surfactants or antistatic agents, etc. So they are used everywhere where protection against microbial infection is required and where virucidal, fungicidal (or algicidal) protection is required. Use e.g. in healthcare, social services, animal production where disinfection of surfaces and surfaces is commonly used, then for example in the food industry - in the production and preparation of any food and beverages (including storage areas) and of course in epidemic situations, both on surfaces and surface, as well as in the form of fogging - spray (so-called spatial disinfection).

As for the quaternary ammonium compound contained in this disinfectant, i.e. DDAC, Didecyldimethylammonium chloride (it is odorless), in the given concentration (table p. 5) it has a fundamental effect against microorganisms and bacteria, including enveloped viruses, and then, of course, bactericidal effects , fungicidal. This means that, for example, achieving a virucidal effect is very effective (according to EN standards), including virucidal and fungicidal properties.

DDAC is a versatile and widely used biocide. It also has - insecticidal and algicidal effects. It is also used in horticulture to decontaminate tables and tools in greenhouses. DDAC is a product approved by the European Union as a bactericide, fungicide, herbicide, insecticide and algicide.

Article 1

ECHA - EUROPEAN CHEMICAL AGENCY

Didecyldimethylammonium chloride

Source: httpsů/echa.europa.cu/cs/substance-information/-/substancejnfoZ100 ₂ 027 _J 751 (Citation - We are the European Chemicals Agency, an EU agency. We implement EU legislation on chemicals to protect your health and environment. Our work also contributes to the proper functioning of the EU internal market and supports the innovation and competitiveness of the European chemical industry.)

This substance (DDAC) is registered under REACH and is manufactured or imported into the European Economic Area in quantities of > 100 to < 1,000 tonnes per year. This substance is used by consumers, professional workers (extended use), in formulation or repackaging, in industrial plants and in manufacturing.

Biocidal use:

This substance is approved for use as a biocide in the EEA and/or Switzerland for: human hygiene, disinfection, veterinary hygiene, food and animal feed, wood preservation. This substance is being reviewed for use as a biocide in the EEA and/or Switzerland for: product preservation, building materials preservation, liquid systems preservation, slime control.

Consumer use:

This substance is used in the following products: biocides (eg disinfectants, pest control products), washing and cleaning products, plant protection products and cosmetics and personal care products.

- 2 CZ 37205 U1

Further release of this substance into the environment is likely to occur from: internal use as excipient and external use as excipient.

Battery life:

ECHA has no publicly registered data on the routes by which this substance is most likely to be released into the environment. ECHA has no publicly registered data indicating whether or into which objects the substance may have been processed.

Widely used by professional workers:

This substance is used in the following products: biocides (eg disinfectants, pest control products), washing and cleaning products, plant protection products and water treatment chemicals. This substance is used in the following areas: mining and quarrying. This substance is used to make: chemicals.Other releases of this substance into the environment are likely to occur in: indoor use (e.g. washing liquids/detergents in washing machines, car care products, paints and coatings or adhesives, fragrances and air fresheners ) and outdoor use.

Formulation or Repackaging:

This substance is used in the following products: biocides (eg disinfectants, pest control products), detergents and cleaners, water treatment chemicals, polishes and waxes, pH regulators and water treatment products, air care products, chemicals and paper dyes and cosmetics and personal care products.

Release of this substance into the environment may occur during industrial use: formulation of mixtures.

Use in industrial areas:

This substance is used in the following products: biocides (eg, disinfectants, pest control products), detergents and cleaners, plant protection products, water treatment chemicals, and oil and gas exploration or production products.

Article 2

Didecyldimethylammonium chloride chemical compound / From Wikipedia, the free encyclopedia Source: https://www.wikiwand.com/cs/Didecyldimethylammoniumchlorid

Didecyldimethylammonium chloride is a quaternary ammonium compound with the chemical formula (CioH ₂ i) ₂ (CH ₃ ) ₂ NCl, used as an antiseptic, disinfectant, fungicide, emulsifier, catalyst and antistatic agent (detergent).

DDAC is a universal and widely used biocide. It has fungicidal, bactericidal and algicidal effects. For example, it is used in horticulture to decontaminate tables and tools in greenhouses. DDAC is a product approved by the European Union for plant protection - however, it can be used (as a bactericide, fungicide, herbicide and algicide) only in an indoor environment on ornamental plants.

DDAC is also used in wood preservatives and generally in anti-algae products. The use of DDAC in biocidal products of type 16 (molluscicides) and type 18 (insecticides, acaricides and preparations to control other arthropods) has been authorized in the EU since January 2008.

However, by the decision of the European Commission, DDAC was not included in the list of permitted substances for the use of type 13 (preservatives for liquids used in metalworking), because no one showed interest in taking the role of a participant in the DDAC review program for the given type of product within the specified period. DDAC is also used in many household and healthcare cleaning and disinfecting products. In the field of cosmetics, it can be used in hair conditioners because it is well absorbed and has antistatic effects.

- 3 CZ 37205 UI

The essence of the technical solution

The subject of this technical solution is the Surface premium disinfectant (concentrate solution), which can be used against a range of bacteria, viruses, fungi and molds and is intended for the disinfection of surfaces and areas, including the form of fogging, which is important for preventing the spread of pathogenic (disease-causing) microorganisms . This concentrate is diluted with water 1:100 when used.

Therefore, it is suitable in places where protection against pathogenic (disease-producing) microorganisms - molds - fungi, viruses and bacteria is necessary, e.g. in food production, animal production, storage of fruit and vegetables, etc. Then in hospitals, social services, etc. Surface preparation premium is intended for professional use.

Didecyldimethylammonium chloride is an antiseptic disinfectant that is used in this biocidal application, where it causes disruption of intermolecular interactions and dissociation of lipid bilayers, thereby increasing the virucidal and bactericidal effectiveness of the preparation.

As for bacteria - the cationic polymer attracts the negatively charged cell membrane of the microorganism, which is disrupted in contact with the polymer, in the case of a virus, it destroys the viral capsule.

Polymer disinfection (based on KAS - quaternary ammonium compounds) is not only effective, it can remove fungi, bacteria and viruses from the premises, but at the same time it protects the treated surfaces from the re-emergence of fungi.

For this purpose, this disinfectant composition was developed - a concentrate 1:100 (disinfectant, solution), the essence of which is that it contains the quaternary ammonium compound didecyldimethylammonium chloride - 5.8 g/per 100 g of demineralized water (see example 3).

Furthermore, the preparation includes propan-2-ol (isopropyl alcohol) with the chemical formula C3H8O. Isopropyl alcohol is an isomer of propyl alcohol with antibacterial properties, at the same time propan-2-ol acts as a detergent, i.e. a cleaner-degreaser (it has excellent cleaning and degreasing capabilities and removes moisture) - 2.79 g/per 100 g of demineralized water (example 3) .

According to the implementation of the technical solution (example 3), the disinfectant composition further includes one auxiliary substance selected from the group containing essential oils - lemon grass (lat. Cymbopogon citratus) - 0.08 g/per 100 g of demineralized water (example 3).

This substance has an antibacterial, antiviral and antifungal effect. Lemon grass has a sweeter and stronger lemon scent - it contains antioxidants, flavonoids and phenolic compounds such as luteolin, glycosides, quercetin, kaempferol, elimicin, catechol, it also contains chlorogenic acid and caffeic acid.

Lemongrass essential oil has antimicrobial and anti-bacterial properties that help fight infections caused by various pathogens such as Helicobacter pylori and Escherichia coli.

The balance up to 100% of the weight of this disinfectant consists of - demineralized water.

The disinfectant product according to the technical solution (see example 3 - selected optimal composition) showed a high disinfection efficiency according to the performed tests (Efficiency Tests). The advantage of this

-4CZ 37205 U1 of the technical solution is high - virucidal, microbial and fungicidal efficiency.

Another advantage of this solution is also - detergent (cleaning effect) effect. This means it can be used as a very effective disinfectant cleaner. At the same time, this preparation does not smell (e.g. of chlorine - chlorine-based disinfection) and is not aggressive to materials - alcohol.

The slightly alkaline (alkaline) setting of Surface premium protects surfaces. This disinfection does not contain chlorine and other irritating substances (e.g. alcohols), is suitable and gentle on all types of materials, with the fact that it does not damage surfaces (such as alcohol disinfectants), is non-corrosive, and the permanent color of the treated materials is ensured.

According to official regulations, cleaning and disinfecting agents must be rinsed off without residue or rinsed with water of drinking water quality from surfaces that come into contact with food.

According to article 1, on page 2 - thanks to DDAC (Didecyldimethylammonium chloride), this preparation also has the ability to control animal pests according to types of preparations - ECHA: types of preparations (ECHA - EUROPEAN CHEMICAL AGENCY)

Formulation or Repackaging:

This substance is used in the following products: biocides (eg disinfectants, pest control products), detergents and cleaners, water treatment chemicals, polishes and waxes, pH regulators and water treatment products, air care products, chemicals and paper dyes and cosmetics and personal care products.

Examples of implementing a technical solution

For this disinfection, we focused mainly on fulfilling the functionality according to the conditions and standards for disinfectants according to the Technical Standards (the Standards are detailed on page 5, including system effects):

- EN 17272:2020

- EN 14476+A2:2020

- EN 17387:2022

Description of Technical Standards and Testing Methods - detailed in this chapter below. Effectiveness testing was carried out by an accredited workplace of the Ministry of Health - Health Institute in Ostrava (department of clinical laboratories - testing of biocidal substances) - Partizánské nám. 2633/7, 702 00 Moravská Ostrava and Přívoz.

The optimal time of action after careful application is within 30 minutes for this mixture according to individual EN standards and based on the results of efficiency tests. The product is intended for professional use. It is a so-called concentrate, which is subsequently diluted with water in a ratio of 1:100 and is then ready for manual application to surfaces and areas for the purpose of disinfection, or also in the form of spatial disinfection - by fogging using foggers (mist generators) and spraying, so-called mist disinfection, or so-called automated disinfection.

Example 1

Preparation with subsequent composition of the disinfectant product for surfaces and surfaces (concentrate): All listed substances (substances/raw materials) are in liquid form and are soluble in cold water. The raw materials listed below were mixed at temperatures ranging from 19 to 22°C.

The raw materials listed in the table were dissolved at room temperature in demineralized water to form a disinfectant solution.

The disinfectant composition (concentrate) suitable for surface disinfection is prepared by dissolving the complexing agent - 5.1 g of the quaternary ammonium compound didecyldimethylammonium chloride and propan-2-ol - 2.48 g, in demineralized water, see the table of examples on page 5.

Subsequently, essential oil (lemongrass) - 0.04 g, again in liquid form, is added to the solution

- 5 CZ 37205 U1 in demineralized water, see the table of examples on page 5. The result of this process is a clear, homogeneous solution that has a very faint hint of perfume, (no alcohol smell) and the pH of the resulting solution is around pH values 7.8.

Example 2

Preparation with subsequent composition of the disinfectant product for surfaces and surfaces (concentrate): All listed substances (substances/raw materials) are in liquid form and are soluble in cold water. The raw materials listed below were mixed at temperatures ranging from 19 to 22°C.

The raw materials listed in the table were dissolved at room temperature in demineralized water to form a disinfectant solution.

The disinfectant composition (concentrate) suitable for surface disinfection is prepared by dissolving the complexing agent - 5.3 g of the quaternary ammonium compound didecyldimethylammonium chloride and propan-2-ol - 2.64 g, in demineralized water, see the table of examples on page 5.

Subsequently, essential oil (lemongrass) - 0.06 g is added to the solution, again in liquid form in demineralized water, see the table of examples on page 5. The result of this process is a clear, homogeneous solution that has a very faint hint of perfume, (without smell of alcohol) with the fact that the pH of the resulting solution is around pH 7.8.

Example 3

Preparation with subsequent composition of the disinfectant product for surfaces and surfaces (concentrate): All listed substances (substances/raw materials) are in liquid form and are soluble in cold water. The raw materials listed below were mixed at temperatures ranging from 19 to 22°C.

The raw materials listed in the table were dissolved at room temperature in demineralized water to form a disinfectant solution.

The disinfectant composition (concentrate) suitable for surface disinfection is prepared by dissolving the complexing agent - 5.8 g of the quaternary ammonium compound didecyldimethylammonium chloride and propan-2-ol - 2.79 g, in demineralized water, see the table of examples on page 5.

Subsequently, Essential Oil (Lemongrass) - 0.08 g is added to the solution, again in liquid form in demineralized water, see the table of examples on page 5. The result of this process is a clear, homogeneous solution that has a faint hint of perfume, (odorless after alcohol) with the fact that the pH of the resulting solution is around pH 7.8.

Example 4

Preparation with subsequent composition of the disinfectant product for surfaces and surfaces (concentrate): All listed substances (substances/raw materials) are in liquid form and are soluble in cold water. The raw materials listed below were mixed at temperatures ranging from 19 to 22°C.

The raw materials listed in the table were dissolved at room temperature in demineralized water to form a disinfectant solution.

Disinfectant composition (concentrate) suitable for surface disinfection is prepared by dissolving the complexing agent - 4.9 g of the quaternary ammonium compound didecyldimethylammonium chloride and propan-2-ol - 2.34 g, in demineralized water - up to 100 g.

Subsequently, essential oil (lemongrass) - 0.03 g, again in liquid form, is added to the solution. The result of this process is a clear, homogeneous solution that has a faint hint of perfume (no alcohol smell), with the pH of the resulting solution hovering around pH 7.8.

- 6 CZ 37205 U1

Based on the example table below, the optimal composition from column 3 was selected.

raw material (g/100g concentrate) 1 2 3 4 didecyldimethylammonium chloride (DDAC) 5.1 5.3 5.8 4.9 propan-2-ol (isopropyl) 2.48 2.64 2.79 2.34 essential oil (lemongrass) 0.04 0.06 0.08 0.03 water (demineralized) up to 100 g up to 100 g up to 100 g up to 100 g

In particular, the composition of the compositions according to the example in column no. 2 and 3 showed, with a duration of exposure of 30 min., in the given concentrations (see the table above), a very strong disinfection efficiency against the tested microbes from the group of gram-negative and gram-positive bacteria - Staphylococcus aureus (also golden staphylococcus, is gram-positive bacteria belonging to the staphylococci genus), Pseudomonas aeruginosa (belongs to gram-negative, aerobic, motile, non-fermenting bacteria), Escherichia coli (originally called Bacterium coli, is a gram-negative, facultatively anaerobic, non-spore-forming rod-shaped bacterium) and also good fungicidal effectiveness - microscopic filamentous fungus and mold.

The basic (selected) composition of the compounds from the table - column 3, had a significant effect on the efficiency spectrum, as the cationic polymer attracts the negatively charged cell membrane of the microorganism, which is disrupted in contact with the didecyldimethylammonium chloride (DDAC) polymer, in the case of a virus, it destroys the viral capsule.

Selected example - column 3:

Preparation with subsequent composition of the disinfectant product for surfaces and areas (concentrate): All the listed substances (raw materials) are in liquid form and are soluble in cold water. The above raw materials were mixed at temperatures ranging from 19 to 22 °C.

The optimal time of action after careful application is no later than 30 minutes for this mixture. The product is intended for professional use.

It is a so-called concentrate, which is then diluted with water in a ratio of 1:100 and then prepared for manual application to surfaces and areas for the purpose of disinfecting surfaces and surfaces, possibly also in the form of spatial disinfection - by fogging with foggers (mist generators). by spraying, so-called mist disinfection, or for spraying with automatic systems.

When testing disinfection effectiveness, we focused on testing methods - bactericidal, virucidal and fungicidal effectiveness for the areas defined below according to individual Technical Standards (EN).

Methods of testing the product for - virucidal, bactericidal and fungicidal effectiveness (by the Institute of Health in Ostrava):

1. according to the Technical Standard - EN 14476+A2:2020

Chemical disinfectants and antiseptics - Quantitative test using a suspension to determine the virucidal effect in the health sector. Testing method and requirements (Phase 2 / Level 1). Tested to a stricter healthcare standard.

2. according to the Technical Standard - EN 17272:2020 (three methods were chosen to determine - fungicidal, virucidal, bactericidal effectiveness)

Chemical disinfectants and antiseptics - Methods of disinfecting airborne spaces by automated process.

Determination of bactericidal, mycobactericidal, sporicidal, fungicidal, yeasticidal,

- 7 CZ 37205 U1 virucidal and phagocytic activities.

The test methods described are designed to determine the disinfection activity of processes used in 1) the medical field,

2) veterinary areas, 3) food, industrial, domestic and institutional areas using automated chemical distribution processes by air diffusion without the manual use of an operator.

3. according to the Technical Standard - EN 17387:2022

Chemical disinfectants and antiseptics - Quantitative test to evaluate the bactericidal and anti-yeast and/or fungicidal activity of chemical disinfectants used in the health sector on non-porous surfaces without mechanical action. Testing method and requirements (phase 2 / grade 2).

Tested samples are considered 100% and were diluted with sterile distilled water to the desired concentrations before use.

Industrial applicability

This preparation belongs to disinfectants (concentrates) to be diluted 1:100 with water, intended for surfaces and surfaces, including fogging and also for automatic disinfection of rooms and closed spaces.

It is a disinfectant for complex disinfection of rooms and surfaces by application to surfaces and surfaces, including fogging. In the case of fogging, the disinfectant is sprayed in a cold application and blown into the air as an aerosol. This process disinfects all accessible surfaces and the air itself, thus achieving complete 3D disinfection.

This product is used wherever protection against fungicides (molds, fungi) is required, further against microbial infection and where virucidal protection is required. This means that it is highly effective against bacteria, viruses, fungi and spores. It is also used for automatic disinfection of rooms and closed spaces - e.g. in animal production, etc.

Furthermore, this agent is used through the fogging method, when a fog is created that penetrates even hard-to-reach places under pressure. In this way, the sprayed disinfectant solution settles on the treated surfaces, which it disinfects just like the air in the given space.

The diluted solution is used for quick disinfection of surfaces, objects, surfaces and hard-to-reach places by fogging (fogging). This application achieves a high quality of disinfection with a low consumption of disinfectant solution.

The preparation is also suitable for treating walls, ceilings, floors and other parts of spaces intended for storage, and thus ensures the possibility of long-term storage, for example - in warehouses of fruit, vegetables, potatoes, etc. It is also possible to disinfect - crates, crates, tools, tools and other aids. In addition to being used against mold on walls, it is very suitable for effective disinfection of, for example, showers and wellness facilities.

Since this spatial disinfection is highly effective against bacteria, viruses, fungi and spores, this disinfection can be used for spaces and surfaces - veterinary hygiene, hospitals, social services, and then, for example, in laboratories and the food industry and other fields, including epidemic situations .

• eliminates a wide range of microorganisms • has bactericidal effects (up to 30 min), virucidal effects (up to 30 min) and fungicidal effects (up to 30 min) • the agent is characterized by a strong and long-lasting antibacterial, antiviral and antifungal effect without negative effects on any treated materials (glass,

- 8 CZ 37205 U1 wood, metals, plastic, textiles, rubber, leather and leatherette, stone) i.e. that it does not damage materials, is non-corrosive • slightly alkaline (alkaline) setting of the product - Surface premium protects surfaces • does not contain chlorine, alcohol, aldehydes or phenols • does not stain and is odorless

Claims (3)

1. Disinfectant for surfaces and surfaces characterized by the fact that it contains the quaternary ammonium compound didecyldimethylammonium chloride DDAC in combination with propan-2-ol, i.e. 5 with isopropyl alcohol, and demineralized water. 2. Disinfectant according to claim 1, characterized in that it contains at least one substance selected from the group containing essential oil - Lemongrass oil - lemongrass. 3. The disinfectant according to claim 1 and 2, characterized in that it is in the form of a solution.