CZ2016820A3 - A device for facilitating the handling of solvents, mixtures and samples on carriers, a kit for handling them, and the use of the device and/or the kit - Google Patents

Abstract

Devices for facilitating the handling of solvents, compositions, and samples on carriers include a holder, a cap, an insert, and at least one device, wherein: the holder comprises a substantially cylindrical-shaped sample portion having a recess for receiving the insert, wherein the lower portion of the recess is provided with at least one aperture and wherein the outer shell of the sample holder portion is threaded in at least one portion thereof and wherein in this threaded portion the outer shape of the holder is substantially a roll; the insert consists of an outer portion and an inner portion, the outer portion of the insert being provided with an opening for the inner portion of the insert connecting the opposing supports of the outer portion of the insert, the inner portion of the insert being provided with at least one opening connecting its opposing supports, and wherein the outer shape of the outer portion of the insert is sized and the shape of the recess of the sample holder portion and the outer shape of the inner portion of the insert is adapted to the opening in the outer portion of the insert at least in a portion of the inner portion of the insert and at least in the portion of the inner portion of the insert is greater than the narrowest point of the opening in the outer portion of the insert; the cap comprises a substantially cylindrical-shaped recess, wherein the inner shell of the lid recess is provided with a thread compatible with the thread on the outer shell of the sample portion of the holder in at least one portion thereof, and wherein the lid portion opposite the lid portion includes a recess at least one opening. preferably circular; wherein a portion of the lid around the aperture or openings in the lid in the region facing the lid recess is at least in some portion greater than a plan view of the outer portion of the insert and / or the devices located in the device; the device is adapted to fit into the sample portion of the holder in shape and size, and is selected from the group consisting of filters, washers, gaskets, separation portions, sample carriers, or combinations thereof.

Description

Technical field

The present invention relates to a device for facilitating the handling of solvents, mixtures containing liquids and sample operations on carriers, sets for handling solvents and mixtures containing liquids and sample operations on carriers and their use. It makes it possible to work in virtually any container with a substantially hollow cylindrical neck and allows various operations with substances such as filtration, particle sedimentation, sample incubation or sample washing along with various aids to be introduced into the device. Its primary use is directed to the area where centrifugal and vacuum forces are used for processing.

BACKGROUND OF THE INVENTION

There are a number of devices for working with solvents and mixtures. Most often it is a device allowing filtration, sedimentation or washing of samples. Examples of such devices are tubes fitted with filters inside to allow filtered mixtures to be obtained, or relatively complex systems for cytocentrifugation. These are used to capture cells on the mats, most commonly the glass panes. Other devices allow incubation of samples such as histological sections with dye solutions as well as washing of the samples in individual baths. In general, a number of different instrumentation solutions have been developed, generally allowing only selected applications, eg only filtering or attaching particles, eg cells, to the surface.

SUMMARY OF THE INVENTION

The present invention relates to a device for working with solvents, mixtures containing liquids (hereinafter mixtures) and sample operations on carriers which can advantageously be used in conjunction with containers with a substantially hollow cylindrical neck. Aids can be loaded into the device that, when placed in the device, allow various handling of solvents, mixtures, and sample operations on carriers. Examples of manipulations are eg filtration, sedimentation or washing of samples with mixtures and washing of samples on carriers. In these operations, predominantly centrifugal or gravitational forces or vacuum suction forces are used, although the device allows the use of other types of suction, for example by pipette. Examples of processed samples are, for example, cell culture media or solutions containing solid particles. The samples may also be particles or other materials adhered to the surfaces of the carriers that are embedded in the apparatus, and subsequently the apparatus is used, for example, to wash them. Thus, one device supplemented with aids, which may also be a solid, integral part of the device, may be used for various handling of solvents, mixtures and / or samples on carriers according to the respective device. • •

operate in containers with a substantially hollow cylindrical neck, preferably in centrifuge tubes, and also collect the solvents and compositions processed by the devices. The device comprises a cap, liner, holder and at least one device. The lid and the bracket are threaded and comprise a bore or bores in the vertical axis. The thread serves to connect both parts and apply pressure to the insert located inside the holder and to the at least one device also located inside the holder. This fixes the insert and the device (s). Some devices may be an integral part of the liner, such as filters and seals. Preferably, the devices are used separately. The devices in combination with the device allow individual operations with fabrics. Aids include filters and seals such as washers or separators or sample carriers. For easier handling, the device can be equipped with a stand.

It is an object of the present invention to provide a device for facilitating the handling of solvents, compositions and samples on carriers comprising a holder, an insert, a lid and at least one aid, wherein:

the holder comprises a substantially cylindrical sample portion provided with a recess for receiving the insert, the lower portion of the recess having at least one opening adapted to drain the solvent-treated device and the mixture from the sample portion of the holder, the outer shell of the sample portion of the holder being at least the threaded portion, and in the threaded portion the outer shape of the holder is substantially a cylinder;

- the liner consists of an outer portion and an inner portion, the outer portion of the liner having an opening for the inner portion of the liner connecting opposing bases of the outer portion of the liner, the inner portion of the liner having at least one opening connecting its opposing bases; the shape of the recess of the sample holder part and the outer shape of the inner liner is at least in part of the inner liner adapted to the opening in the outer liner and at the same time is at least in the inner liner larger than the narrowest point of the opening in the outer liner container of solvents and mixtures. The liner may further comprise aids, such as a filter and / or a seal, which are permanently attached to the liner.

- the lid comprises a substantially cylindrical recess, the inner lid of the lid recess having at least one portion thereof having a thread compatible with the thread on the outer shell of the sample portion, and wherein the lid portion opposite the lid portion containing the recess is provided with at least one hole; preferably circular; and wherein the portion of the lid around the aperture or apertures in the lid in the region facing the recess of the lid at least some of it extends beyond the plan view of the outer portion of the liner and / or the devices disposed in the device, and wherein at least one of the apertures allows the sample portion to be filled with solvents and / or mixtures;

the device is shaped and sized to be inserted into a sample portion of the holder, and is selected from the group consisting of filters, washers, seals, separators, sample carriers, or combinations thereof.

The holder or its sample part provides the bottom for the insert and any other aids placed into the device and together with the lid fixes them in the necessary positions, the holder always at least partially protrudes from the lid towards the lid when screwed into the lid. the container on which the device is placed to allow for easy screwing in and out.

The compositions may include, but are not limited to, various solvents or solutions containing, for example, protein components, whole cells or residues thereof, or nucleic acids, or nanoparticles, or mixtures thereof. The pads also include sample carriers. Specimen carriers are understood to be, for example, glass, metal, paper or plastic parts, most often in the form of plates of different thickness, which carry samples on their surface or are intended to receive samples that are firmly bound to the surface of the carrier during use. Samples adhered to the supports may be, for example, cells, histological sections, or various particles such as nanoparticles.

The thread on the holder and the lid allows the lid to be connected to the holder.

A preferred holder arrangement comprises a drainage orifice located at the center of the bottom of the sample portion of the holder. The drain hole can be used to connect to the pump by other elements, such as a hose. Advantageously, the bottom may be roughened or provided with drainage channels to rapidly drain solvents and / or mixtures.

For ease of handling and tightening, preferably at least a portion of the surface of the holder portion is provided with serrations or otherwise roughened. For ease of handling, it is advantageous to use an extension in the form of a ring that can be located anywhere below the thread end of the holder portion. The ring may be serrated or otherwise roughened.

The liner serves as a reservoir of solvents and / or mixtures that are used during sample processing, and at the same time, especially in an embodiment such as having a filter and / or seal, allows some operations with the solvents and / or mixtures to be treated. The insert is pressed onto the other inner part of the device (aids) by means of a threaded connection between the lid and the holder. The outer shape of the insert depends on the shape of the recess in the sample portion of the holder. The insert must fit into the recesses in the sample part of the holder. In order to prevent the insert from twisting when the lid is tightened, it is preferred that the plan view of at least some of the insert portion abuts the recess wall of the sample holder portion and prevents it from rotating. From this point of view, the preferred plan view of these parts corresponds to a polygon, a circle, optionally an ellipse or a circular segment. If the insert is essentially a cylinder in all parts, it is preferable to provide the insert on the casing with grooves or projections that prevent it from twisting in the sample portion of the holder and fit into complementary elements in the sample portion of the holder. It is irrelevant whether these elements will be placed on the shell of the inner part of the insert or the outer part of the insert, or both.

The shape of the hole or holes of the inner part of the insert connecting its bases is not critical, however their plan and possible distribution in the base, which is closer to the bottom of the specimen part of the holder defines »

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For example, the extent and position at which the particles will be sedimented on the carrier will be applied. The shape of the opening in the outer part of the liner may be different, preferably it is essentially a cylinder, an ellipsoidal cylinder, a prism or truncated cone or truncated pyramid. The shape may also be a combination of said shapes. It is also possible to combine different prisms, truncated cones or truncated pyramids or cylinders and cylinders with an ellipse-shaped base of different diameters.

The inner portion of the insert is made in an external shape that allows at least a portion of its insertion into an opening in the outer portion of the insert and at the same time prevents it from completely passing through the opening of the outer portion of the insert. Such a shape is essentially a truncated cone or truncated pyramid or a combination thereof with prisms and cylinders or cylinders with an ellipse-shaped base. A preferred shape is a combination of different prisms or cylinders of different diameters, or a combination of cylinders and prisms, or a combination of cylinders, truncated pyramids, truncated cones and prisms with shapes having a circular section or ellipse in plan view.

A particularly preferred example of the shape of the inner portion of the liner is a set of two rollers which adjoin their bases and share a common axis of symmetry. In this case, the complementary shape of the opening in the outer portion of the liner is either a cylinder or a set of two cylinders.

In the case of a single cylinder opening, the inner liner is made such that the diameter of the smaller diameter cylinder is smaller than the diameter of the outer diameter of the liner, and the larger diameter cylinder is larger than the diameter of the outer diameter of the liner. This ensures that the inner part of the liner can be partially, but not completely, inserted into an opening in the outer part of the liner (a larger diameter cylinder prevents complete insertion).

In the case of a two-cylinder aperture, the diameter of the cylinder having a smaller diameter of the liner is smaller than the diameter of the cylinder having a smaller diameter forming an opening in the outside of the liner, and the cylinder having a larger diameter the larger diameter forming the opening in the outer portion of the liner and at the same time being larger than the diameter of the smaller diameter forming hole in the outer portion of the liner. A seal may be used to seal the space between the inner and outer parts of the liner. The seal may be a fixed part of the inner or outer part of the liner, preferably separated from these parts. The use of seals is advantageous, for example, when using vacuum pumps for work with solvents and mixtures.

Preferably, the region of the inner liner that is shaped and sized to pass through the opening of the outer liner is longer than the height of the outer liner and preferably has a smaller diameter than the opening in the lid. This region then preferably passes through an opening in the lid and forms the neck of the insert. The neck of the liner may be provided with a thread for the cap, e.g. to prevent solvent evaporation. Preferably, the inner portion of the insert is provided at its edge closer to the lid opening with a recess or roughening that facilitates handling of the insert. In some cases, it is advantageous to provide the inner part of the insert on its base closer to the bottom of the sample portion with roughening. This is especially the case when the equipment is used for washing samples. It is advantageous to use other aids, in particular seals or separating parts, which are interposed between the outer part of the insert and the lid. This method allows one insert to be used for various devices and their assemblies. The seals or filters may be a fixed part of the inner and outer part of the liner.

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The lid serves to close the device, allows the device to be filled with solvents and / or mixtures, optionally allows the removal of solvents and / or mixtures, also prevents the device from falling into the container and together with the holder and liner serves to fix devices such as seals and filters in desired positions . In preferred embodiments, the cap fixes the device to the neck of the container even against lateral displacement relative to the neck of the container and prevents evaporation of solvents and / or mixtures from the container. The thread on the cap may be either over the entire inner surface of the recess or in only a portion thereof. The opening in the lid portion opposite the lid portion containing the recess serves to apply solvents or / or mixtures, optionally to remove them. The area around the opening or holes forms its ceiling, which serves to fix the inner parts by applying pressure against the bottom of the holder part.

Preferably, the opening is in the form of a cylinder whose diameter is less than the diameter of the threaded cylindrical recess and the center of its base coincides with the center of the threaded cylindrical recess base in the vertical direction. Further, the inseparable part of the lid may be a seal which is disposed around the opening (s) on the lid ceiling and faces towards the recess and / or a separation piece situated similar to the seal.

they may be non-detachably connected or made of one piece of material. The cap may be used to connect the pump via other elements, such as a substantially hollow cylindrical necked receptacle or hose, or via a thread and adapter provided with a compatible thread.

In some embodiments, the liner is made releasably from the outer and inner parts, in others it is made non-releasably, for example, from a single piece of material. An insert is referred to as a set consisting of a separable inner and outer part of the insert and an insert made of a single piece of material, possibly obtained, for example, by gluing the inner and outer parts of the insert.

In a preferred embodiment of the present invention, the holder further comprises a collecting portion which is substantially in the form of a hollow cylinder or two interposed hollow cylinders, wherein the hollow cylinder or the inner of the two interposed hollow cylinders has the same or greater inner diameter than the hole diameter in the sample base. Preferably, the diameter is the same as the diameter of the opening in the base of the sample portion of the holder, the collection portion of the holder being connected to the lower base of the sample portion of the holder. The orifice (cavity of the inner hollow cylinder) is adapted to drain the solvent and / or mixture from the sample portion of the holder and may serve for connection to the pump.

The collecting part is particularly advantageous when the device is of smaller dimensions, because of the increased surface area it makes the device easier to handle. Increasing the total area also improves user comfort by increasing the area, which is well protected from contact with the solvents and / or mixtures used.

In the case where the collecting part is formed by two substantially hollow cylinders, one preferably fits the outlet opening of the bottom of the sample portion of the holder and the other has an outer diameter similar to the outer diameter of the sample portion in the bottom region. Preferably, the sample portion and the holder collection portion are attached non-detachably, more preferably they are made from a single piece of material. The thread provided with the sample portion can also continue on the collection portion of the holder. Preferably, the ring, which in some embodiments is part of the sample portion, is placed on the collection portion. The manifold may be used to connect the pump through other elements, such as a hose or via a thread and adapter provided with a compatible thread.

In some embodiments of the device of the present invention, the inner and outer portions of the liner are non-detachably connected to each other, for example, formed from a single piece of material or glued together. In these embodiments, the insert may also include glue and / or filters that are glued. In some embodiments, the filter having a rigidly attached filter includes aperture (s) in its plan view, in other embodiments it is free of aperture (s). The aperture or apertures are preferably at locations where the aperture or apertures are in the inner portion of the insert. The gasket, which in some embodiments is a fixed part of the liner and, after assembly of the device, seals the portion between the lid and the liner, comprises a plan view to allow filling of the liner.

In other embodiments, the single liner is formed in the form of a cylinder with an aperture or apertures that connect its bases. Preferably, this embodiment has protrusions and / or grooves on the outer shell that are complementary to the protrusions and / or grooves in the sample portion and prevent it from twisting when the lid is tightened.

In another embodiment of the device according to the present invention, the insert is comprised of an inner and an outer portion of the insert, these being detachably connected as two modularly interlocking pieces.

Such an embodiment is, for example, an arrangement in which the inner part of the liner is substantially in the form of two cylinders adjacent to its bases, which share an axis of symmetry and through which a common opening passes. The outer portion of the insert is substantially in the form of a hollow cylinder. The inner liner is made of two coaxial cylinders such that the outer diameter of the smaller diameter cylinder is smaller than the diameter of the opening in the outer liner and the diameter of the larger diameter cylinder is substantially the same as the diameter of the outer liner. The cylinder with a smaller diameter of the inner part of the liner preferably has a smaller diameter than the opening in the lid and forms a throat that extends through the opening in the lid. The outer part of the insert fixates the insert in the recess of the sample portion of the holder. The neck of the insert may be provided with a thread for the cap, eg to prevent evaporation of solvents and / or mixtures. Preferably, the inner portion of the insert is provided at its upper edge with a recess or roughening that facilitates handling of the insert. The inner part of the insert may, in some embodiments, preferably be provided with protrusions, for example in the form of a protruding hollow cylinder, on a base facing the bottom of the sample portion.

In a preferred embodiment, the device according to the invention comprises a lid in the form of substantially two hollow cylinders of the same or different heights, the two hollow cylinders having one common base comprising an opening, preferably a circular opening, preferably located in the middle of the common base. and wherein the inner shell of the inner hollow cylinder is provided in at least one portion thereof with a thread compatible with the thread on the outer shell of the sample portion of the holder. The base is preferably a hollow cylinder or a plurality of hollow cylinders of different heights with a common axis of symmetry and a common center of the base that is farther from the threaded lid portion.

In a preferred embodiment, the outer surface of the cap and / or the outer surface of the holder is provided with roughening, for example grooves or projections. This roughening makes it easier to connect and disconnect the thread between the holder and the cap.

In a preferred embodiment, the recess of the sample holder portion has a shape selected from the group comprising a cylinder, a prism, preferably a perpendicular prism, an elliptical cylinder, a shape derived from a cylinder whose plan view is a circular section. Typically, the recess is in the form of a substantially perpendicular prism, in particular a cuboid, cube or hexagonal prism, or a cylinder, or may have a substantially elliptical shape in plan view. A preferred embodiment is also a shape in which the recess is shaped in plan view as part of a circle which is at least on one side bounded by its cutting, i.e., circular, segment.

In a preferred embodiment, the inner wall of the specimen portion of the holder is at least partially provided with at least one groove and / or one protrusion, with a substantially polygonal, semicircle or rounded polygon shape having a plan view parallel to the thread axis.

In a preferred embodiment, at least one groove in the sample portion extends through the entire wall of the sample portion of the holder. This groove begins at the upper edge of the sample portion and may extend to the bottom of the sample portion, preferably terminating above the bottom level of the sample portion of the holder. The groove facilitates insertion and removal of specimens and possibly aids into the holder. If it is necessary to prevent the inner portions from rotating and the recess of the sample portion of the holder is cylindrical, this groove can also be used to prevent the specimen and / or inner portions from rotating when tightening the lid, which could result in damage. More preferably, a plurality of anti-torsion system may be used, for example a combination of multiple grooves in which the projections on the insert or other aids inserted into the device fit. The grooves may or may not extend through the entire wall thickness. In general, it is preferable to use shallow grooves in order not to significantly weaken the wall of the sample portion of the holder. It is sufficient to use two grooves on opposite sides of the recess to secure the insert and / or the inner parts (aids) against rotation. Alternatively, recesses of the sample holder portion may be provided with protrusions that engage the groove or grooves in the liner and / or some internal portions. A combination of grooves and projections is also possible. It is also possible to combine the protrusions and / or grooves with, for example, shapes which at least partially prevent the rotation of the self. This is particularly the case where the shape of the recess in the shape of one side of the cut cylinder is chosen to prevent rotation.

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In a preferred embodiment of the device, the outer diameter of the inner liner portion at least in the region of its neck is smaller than the diameter of the lid opening, the height of the inner liner extending through the outer liner opening being greater than the height of the outer liner opening.

The devices have a shape compatible with the internal shape of the recess of the sample portion of the holder. The function of the devices in the device of the present invention is as follows:

types of pads - sample carriers that carry samples or serve to capture them. The pads are most often in the form of basically plates of different heights and different plan shapes. The washers may contain recesses of any shape. In general, it is preferred to use washers with a plan shape that corresponds to the plan view of the bottom of the recess in the sample portion of the holder or the plan view of the bottom of the insert. In a preferred embodiment they are made in the form of low cylinders with a diameter which allows easy insertion into the sample portion of the cylindrical holder. The recesses in the washers are preferably made in the form of substantially cylinders or substantially truncated cones or substantially cones. In some cases, filters are used as pads, which can then perform two functions, the pad function and the filter function. In some preferred embodiments, the washers are roughened or provided with drain channels on the underside. These treatments ensure that solvents and / or mixtures are drained into the containers.

The filters are primarily used for filtering the compositions and, if used, define the points where solvents and / or compositions are removed that enter the sample portion of the holder and then are directed to the drain or drain holes. They can also be used as substrates for trapping particles from mixtures. The filters in the form of wafers can have different ground plan shapes and heights. In general, it is preferable to use filters with plan shapes that correspond to the plan shape of the recess in the sample portion or the portion directly urging the filters. In a preferred embodiment, they are made in the shape of a circle or annulus. The filters may be an integral part of the insert. At the same time, they can be combined into larger units, including units obtained by interleaving with separation parts. These combinations may be joined permanently. In the case of such combinations, seals may be provided on one or both sides in a detachable or non-detachable manner.

Separation parts are used to define distances between individual parts. E.g. define the distance between the pad and the filter. Preferably, their plan shape corresponds to the plan shape of the recess in the sample portion or the portion that directly urges the separation members. They always have a vertical opening or openings that extend through the entire separating part. In a preferred embodiment, they are made in plan shape of an annulus. The separating parts are preferably roughened on one or both contact surfaces for some purposes. The separating part may be an integral part of the lid, serving to define the space between the lid and other parts. It is then preferably situated around the opening or openings in the lid.

The gasket serves to seal the spaces between two parts of the device. In particular, the sealing of the space between the liner and the lid cap or between the outer portion of the liner and the lid ceiling or between the washer and the separating member or between the outer and inner portions of the liner or between the pad and the liner or between

the pad and the inner part of the insert or between the sample carrier and the insert, or between the sample carrier and the inner part of the insert or between the sample carrier and the separating member or between the filter and the sample carrier. Its plan shape preferably corresponds to the plan shape of the parts between which the gasket is inserted. The seal may be an integral part of the insert or lid. If it is an integral part of the lid, then the seal is preferably situated around the opening or holes in the lid.

In a most preferred embodiment, the apparatus useful for retaining particles on the surface of the carrier and / or incubating the samples on the carriers in the various solvents and / or compositions of the present invention comprises a holder in the sample portion of the sample carrier or the non-recessed substrate. the support is further provided with a sample carrier, preferably glass, with or without a sample over which the filter is placed, pressed against the filter holder positioned above the filter insert and optionally a gasket positioned above it, the neck of the inner part of the insert being threaded through a hole in the lid screwed with bracket using thread. Optionally, in order to prevent drying of samples on the carriers, this embodiment comprises a seal situated between the sample carrier and the filter, or optionally a seal and a separating piece between the sample carrier and the filter. Optionally, a seal is inserted between the inner and outer portions of the liner, if detachably connected, to improve vacuum pump performance.

The device according to the invention is made of materials which are sufficiently resistant to the substances (solvents and mixtures) used. Preferably, the lid, holder, inserts and some other aids such as separation parts are made of thermoplastics or metal alloys. Examples of thermoplastics are polyethylene, polyoxymethylene or polytetrafluoroethylene. Advantageously, these materials can also be used to make other aids such as pads. In addition, the washers may advantageously be made of glass or metal and materials which are suitable for making other aids such as filters or seals. Sample carriers can also be made of the same materials as washers. Depending on the application, the substrates may be coated with thin layers of other substances which affect their ability to bind the particles contained in the solution. Examples are poly-D-lysine, 3-triethoxysilylpropylamine or gelatin. The filters are preferably made of cellulose, nitrocellulose, nylon, polyvinylidene difluoride, glass microfibers, polypropylene, polysulfone, cellulose acetate or polytetrafluoroethylene. The gaskets are preferably made of rubbers, preferably silicone rubbers or polytetrafluoroethylene or polyethylene or novoplast or paper. The outer part of the insert is in some embodiments preferably made of rubber. The inserts are in some cases preferably made of other materials such as metals and their alloys.

The present invention also provides a kit for handling solvents and compositions and sample operations on carriers comprising a device for facilitating the handling of solvents and mixtures and sample operations on carriers according to the invention and a container, preferably a container with a hollow cylindrical neck intended for location of the device of the present invention.

Preferably, the lid is shaped such that the outer diameter of the portion containing the threaded recess is at least in a portion thereof facing the neck of the container less than the inside diameter of the container neck (e.g., a 50 ml centrifuge tube). The shape and size of the lid above this portion then prevents the lid from falling into the container. This ensures that at least a portion of the lid extends into the container and at the same time it is possible to fix the lid to the container without using any other fastener. It is also possible to use a lid whose ground plan dimensions are larger than the diameter of the container over the entire vertical axis. The preferred shape of the top of the lid is essentially a cylinder provided with a central hole in the middle and the recess portion is a substantially hollow cylinder having a lower outer diameter than the inner diameter of the container and its vertical axis continuing the vertical axis of the upper portion.

An advantageous variant of the lid design in cases where it is necessary to reduce the height of the lid above the container, to fix the lid to the container as much as possible while preventing evaporation of solvents and / or mixtures from the collecting containers is an embodiment where the lid is made in substantially two hollow cylinders of similar height, the two hollow cylinders having one common base. The inner shell of the inner hollow cylinder is provided in at least one portion thereof with a thread compatible with the thread on the outer shell of the sample portion. The base is preferably a hollow cylinder or a system of interconnected two or more hollow cylinders of different heights with a common axis of symmetry and a common center of the base that is farther from the threaded lid portion.

In these cases, the inner diameter of the outer hollow cylinder under the base is always greater than the outer diameter of the container neck, including any thread, and the outer diameter of the inner hollow cylinder under the base is lower than the inner diameter of the container neck. The ceiling of the space between these hollow cylinders formed by their common base then together with their walls fix the lid on the neck of the container. The described embodiment is advantageous, for example, in the case of the use of commercial 50 ml tubes, which in the predominant part have the shape of a hollow cylinder and differ according to the manufacturer by a thread with similar inner and outer diameters.

The space between the hollow cylinders under the base may be provided with a seal in the ceiling area. The seal is advantageous both in cases where it is necessary to minimize evaporation during centrifugation or washing by gravity, and when the cap serves to connect the pump by means of other elements, such as vessels with a substantially cylindrical hollow neck. This cap design allows the use of one cap for test tubes from different manufacturers, providing high evaporation protection while allowing rapid removal of the device from the container. Furthermore, the cap can be provided on the inner shell of the outer hollow cylinder under the base with a complementary thread to the thread that is fitted with the vessel / test tube. Such a solution is advantageous in situations where it is absolutely necessary to prevent evaporation from the container.

For ease of handling, including tightening, it is advantageous to provide the cap in at least some area of the outer surface of the serration or with any roughening. This is especially the case when the outer shape of the lid is essentially a circle in all its parts.

In particular, the vessel with a substantially hollow cylindrical neck may be a conventional or centrifuge tube. Preferably, the containers are made of materials resistant to the solvents and / or mixtures used and at the same time resistant to centrifugal forces, for example thermoplastics, preferably thermoplastic.

polyethylene and polypropylene, or glass. Single-wall thick-walled vessels with a substantially hollow cylindrical neck with a pump connection valve are suitable for use in vacuum pumps. These containers do not serve for collecting the filtrate, which is sucked away from the containers. Generally, however, the device does not only require connection to the vacuum pumps by vessels. It is also possible to connect it with other elements such as hoses or threads and a compatible threaded adapter.

In one embodiment, the kit of the present invention further comprises a stand comprising a base and a tm that is adapted to be inserted into an opening at the bottom of the sample portion of the holder, optionally through an opening in the collection portion of the holder. The stand makes handling the device easier. The height of darkness is selected such that upon insertion into the opening in the bottom of the holder, at least a partial ejection of the parts which are inserted into the sample portion of the holder is thereby facilitated by the user.

It is also an object of the present invention to use the apparatus and / or kit of the present invention for filtering solvents and / or mixtures, sedimenting particles, washing particles, washing samples adhered to substrates and / or carriers, in particular by centrifugation and vacuum pump.

Filtration

Basic filtration methods:

1) The device is gradually assembled from below from a holder, a washer, a filter with an opening, an insert, optionally a seal, and a lid and placed in the neck of the container. Alternatively, the device is connected to the pump by other elements such as a hose or thread and a compatible threaded adapter. When using vacuum pump filtration, if the liner is made removably from the inner and outer portions of the liner, a seal is preferably inserted between the inner and outer portions of the liner. A mixture is applied to the device. The mixture is then centrifuged or aspirated by means of a vacuum pump. Filtration takes place in the filter area between the pad and the pad. The rest of the mixture remains on the support.

2) The apparatus is gradually assembled from below from a holder, a filter without an opening, an insert, optionally a seal, and a lid and placed in the container neck. Alternatively, the device is connected to the pump by other elements such as a hose or thread and a compatible threaded adapter. When using vacuum pump filtration, if the liner is made removably from the inner and outer portions of the liner, preferably a seal is inserted between the inner and outer portions of the liner. A mixture is applied to the device. The mixture is then centrifuged or aspirated by means of a vacuum pump. The filtration takes place in the area of the filter defined by the opening of the inner part of the insert. The rest of the mixture remains on the filter.

3) The apparatus is successively assembled from below from a holder, a washer, a seal with an opening, a separating piece, a filter with an opening, an insert, optionally a seal, and a lid, and placed in the neck of the container. Alternatively, it is

- 12the device is connected to the pump by other elements such as a hose or thread and an adapter provided with a compatible thread. When using vacuum pump filtration, if the liner is made removably from the inner and outer portions of the liner, a seal is preferably inserted between the inner and outer portions of the liner. A mixture containing particles is applied to the device. The mixture is then centrifuged or aspirated by means of a vacuum pump. Filtration takes place in the filter area between the pad and the pad. The remainder of the mixture remains on the substrate and in the space defined by the gasket and separation member.

Sedimentation

Basic methods of sedimentation:

1) The apparatus is successively assembled from below from a holder, a support that can support the sample carrier or is itself a carrier, or serves as a retaining portion, seal, liner, optionally seal, and cap, and placed in the container neck. A mixture is applied to the device. The mixture is then centrifuged preferably using a swing-rotor centrifuge. The mixture remains in the apparatus during the entire rotation.

2) The apparatus is successively assembled from below from a holder, a pad that can support the sample carrier or is itself a carrier, or serves as a retaining portion, seal, optional separator, aperture filter, liner, optional seal, and cap and placed in the container neck. A mixture is applied to the device. The mixture is subsequently centrifuged, preferably using a pivot rotor centrifuge. The mixture partially remains in the apparatus. The filtrate is collected in a container. The described arrangement is advantageous for preventing sediment from drying out and it is not necessary to use pads provided with recesses.

3) The apparatus is successively assembled from below from a holder, a support that can carry the sample carrier or is itself a carrier, or serves as a retainer, a filter with an opening, inserts, optionally a seal, and a lid and placed in the neck of the container. A mixture is applied to the device. The mixture is then centrifuged preferably using a swing-rotor centrifuge. The filtrate is collected in a container. Usually only a small part of the mixture remains in the form of sediment in the plant. The amount of mixture trapped in the machine depends on the design of the pad. If the pad is provided with a sufficiently large recess, this arrangement also prevents the sediment from drying out.

Repeated incubation and washing of specimens retained on carriers or particles in the specimens

Basic methods for repeated incubation and washing of specimens retained on carriers or particles in specimens:

1) The apparatus is successively assembled from below from a holder, a sample carrier or a pad first, followed by a sample carrier, a seal, optionally a separator, a filter, a liner, optionally a seal, and a lid and placed in the neck of the container. Alternatively, the device is connected to the pump by other elements such as a hose or thread and a compatible threaded adapter. While using • · " ·

13 washing by means of a vacuum pump, in the case where the insert is made removably from the inner and outer parts of the insert, preferably a seal is inserted between the inner and outer parts of the insert. A solvent and / or mixture is applied to the apparatus. The solvent and / or mixture is then centrifuged or aspirated by means of a vacuum pump. The solvent and / or mixture partially remains in the apparatus. This step can be repeated. If high-pass filters are used or omitted or replaced, for example, by roughening the bottom of the liner, it is possible to work without the use of a centrifuge or vacuum pump using only the force of gravity. The described arrangement is advantageous for preventing the sample from drying out.

2) The apparatus is successively assembled from below from a holder, a sample carrier or a substrate, followed by a sample carrier, a filter, an insert, optionally a seal, and a lid and placed in the container neck. Alternatively, the device is connected to the pump by other elements such as a hose or thread and a compatible threaded adapter. When using a vacuum pump, when the liner is made removably from the inner and outer portions of the liner, a seal is preferably inserted between the inner and outer portions of the liner. A solvent and / or mixture is applied to the apparatus. The solvent and / or mixture is then centrifuged or aspirated by means of a vacuum pump. The described arrangement allows highly efficient washing, but does not prevent drying of the sample.

3) The apparatus is assembled from below from a holder, a recess pad, a filter, an insert, optionally a seal, and a lid, and placed in the container neck. Alternatively, the device is connected to the pump by other elements such as a hose or thread and a compatible threaded adapter. When using a vacuum pump, when the liner is made removably from the inner and outer portions of the liner, a seal is preferably inserted between the inner and outer portions of the liner. A solvent and / or mixture is applied to the apparatus. The solvent and / or mixture is then centrifuged or aspirated by means of a vacuum pump. The solvent and / or mixture partially remains in the apparatus. This step can be repeated. The described arrangement is suitable for washing particles obtained directly from the sample in the form of a suspension thereof in the recess of the support.

The device of the present invention is a multifunctional device that, among other things, fully replaces conventional cytocentrifugation approaches. The device is adapted for use with rotors of conventional centrifuges, therefore does not require any special cytocentrifuges, allows subsequent processing of the thus obtained preparations, eg staining with histochemical reagents, immunocytochemical detection of proteins or detection of replication activity by "click chemistry". lines. In addition to commonly used cytocentrifugation methods based on "semi-dry" cytocentrifugation, the device also allows to operate in a completely "wet" way, which is advantageous, for example, if necessary to obtain formaldehyde-fixed preparations. Depending on the shape and size of the recess of the washer, it is possible to work with different sizes of sample carriers, eg glass panes and hence with a different size on which t

- 14— processing of preparations. In the most preferred embodiment for attaching the cells to the support by the "semi-dry" method, the device comprises a holder in the sample portion of which a recessed support is placed in which the specimen support is located, preferably a cover glass over which a filter is pressed against the support. a plurality of inner and outer liners, the neck of the inner liner being threaded through a cap screwed to the holder by means of a thread. The device is preferably intended for centrifuge tubes with a neck having an inner diameter greater than 26 mm and an outer diameter including an optional thread less than 33 mm, which satisfies most 50 ml plastic centrifuge tubes. The inserts and filters have an inside diameter of 5 to 12 mm. In the case of the insert, this is the end diameter. The diameter of the filters and inserts defines the size of the processing area.

The apparatus allows post-treatment of samples, eg washing, incubation in various solvents and / or mixtures etc., not only by centrifugation, but also by suction forces mediated by the vacuum pump.

In the case of using a "wet" processing path, a seal with the required internal diameter, which should correspond to the diameter of the filter and insert used, is placed on a support to capture or process samples such as cells. Preferably, a separating piece of the same diameter as the diameter of the gasket and the diameter of the filter and the insert is then placed thereon. A filter follows. The next assembly procedure is the same as for the semi-dry road construction. The assembled device is suitable for most subsequent applications as it ensures that the solvent and / or mixture is trapped above the formulation and prevents it from drying out. The described assembly is generally suitable for the preparation of formaldehyde-fixed cells. For successful cell capture, it is preferable to work with glass coated cell adsorption enhancers. Examples are poly-D-lysine coated glasses. In this case, it is advantageous to remove the protein sample by repeated washing before application.

All parts outside the filter are preferably made of white Teflon. The device itself (without filter) is made of Teflon resistant to most solvents, acidic and alkaline environment even at higher temperatures and has a heat resistance from -250 to + 250 ° C. Recommended use is at temperatures from -50 to 200 ° C. The device itself can be autoclaved repeatedly (121 ° C, 15 PSI, 30 minutes).

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Brief description of the illustrations

Giant. 1 is a 3D illustration of a threadless cap of Example 1

Giant. 2 is a 3D illustration of a threadless cap of Example 2

Giant. 3 is a 3D illustration of a threadless cap of Example 3

Giant. 4 is a 3D illustration of a threadless cap of Example 4

Giant. 5 is a 3D illustration of a threadless cap of Example 5

Giant. 6 A 3D illustration of the lid of Example 6

Giant. 7 is a 3D illustration of a sample portion without the thread of Example 7

Giant. 8 3D illustration of the sample without thread from example 8 • ·

FIG. 9 is a 3D illustration of a sample portion without the thread of Example 9

Giant. 10 is a 3D illustration of a sample portion without the thread of Example 10

Giant. 11 is a 3D illustration of a sample portion without the thread of Example 11

Giant. 12 is a 3D illustration of a sample portion without the thread of Example 12

Giant. 13 is a 3D illustration of a sample portion without the thread of Example 13

Giant. 14 is a 3D illustration of a sample portion without the thread of Example 14

Giant. 15 is a 3D illustration of a sample portion without the thread of Example 15

Giant. 16 is a 3D illustration of a sample portion without the thread of Example 16

Giant. 17 is a 3D illustration of a sample portion without the thread of Example 17

Giant. 18 is a 3D illustration of a sample portion without the thread of Example 18

Giant. 19 is a 3D illustration of a sample portion without the thread of Example 19

Giant. 20 is a 3D illustration of the collection portion of Example 20 including the bottom area of the sample portion provided with an opening

Giant. 21 is a 3D illustration of the collecting portion S of Example 21 including the bottom area of the sample portion Vz having an opening

Giant. 22 is a 3D illustration of the collection portion of Example 22 including the bottom portion of the sample portion provided with an opening

Giant. 23 is a 3D illustration of the holder of Example 23 composed of the sample portion Vz and the collector portion S. In the left and right sections are two views of the same holder.

Giant. 24 3D illustration of the insert from example 24

Giant. 25 A 3D illustration of the insert of Example 25

Giant. 26 3D illustration of the insert from example 26

Giant. 27 3D illustration of the insert from example 27

Giant. 28 A 3D illustration of the insert of Example 28

Giant. 29 is a 3D illustration of the outer portion of the insert of Example 29

Giant. 30 is a 3D illustration of the inner liner of Example 30

Giant. 31 is a 3D illustration of the inner liner of Example 31

Giant. 32 is a 3D illustration of the inner portion of the insert of Example 32

Giant. 33 A 3D illustration of the pad of Example 33

Giant. 34 A schematic arrangement of a device suitable for trapping particles on the carrier (No) of Example 35. The assembly including carrier (No) and containers (N) comprises a cap (V), a hollow cylinder seal (T), an outer liner (VV), the inner part of the insert (4), the hollow cylinder filter (F), the washer (P) with a recess on the upper side for the carrier (No), as well as a cross recess on the lower side for draining fabrics and a holder (D).

Giant. 35 3D illustration of stand from example 38 • ·

DETAILED DESCRIPTION OF THE INVENTION

Example 1 Cap Design

An exemplary embodiment of a lid of a device wherein the lid has the shape of a cylinder with a cylindrical recess in its lower part and a circular opening in the middle of the upper part of the cylinder (Fig. 1). The diameter of the cylinder is greater than the inner diameter of the container neck. The thread is placed in a cylindrical recess having a diameter smaller than the inner diameter of the container. The lid rests on the top edge of the container and is completely above the container. K. Lateral fixation of the cap occurs only after attachment of the holder that extends into the container.

Example 2 Cap Design

An exemplary embodiment of a lid of a device, wherein the upper part of the lid is in the form of a cylinder with a circular hole in its center (Fig. 2). The upper portion has a larger diameter than the inner diameter of the container. The lower part is in the form of a hollow cylinder. The hollow cylinder has a smaller outer diameter than the diameter of the upper portion and at the same time this diameter is smaller than the inner diameter of the container neck. In this embodiment, the hollow cylinder extends into the container. The lid rests on the upper edge of the container neck. The thread is provided with part or all of the inner shell of the hollow cylinder.

Example 3 Cap Design

An exemplary embodiment of a lid of a device wherein the lid additionally comprises a flange over the lid of Example 2, which together with the hollow cylinder defines a space for the neck of the vessel (Fig. 3). The inner diameter of the skirt is larger than the outer diameter of the container. If the container is provided with an external thread, the flange is larger than the diameter including the thread. The receptacle neck fits into the space between the hollow cylinder and the skirt, the lid rests on the upper edge of the receptacle neck and a recess extends into the receptacle. In some embodiments, the lid of Figure 3 is additionally provided with a thread on the inside of the skirt that is complementary to the thread of the container.

Example 4 Cap Design

An exemplary embodiment of a lid of a device, wherein the lid is distinguished from the lid of Example 3 in that it additionally has a longer flange, allowing a higher fixation of the device to the neck of the container (Fig. 4). Thus, the lid is in the form of substantially two hollow cylinders of the same or different heights, the two hollow cylinders having one common base in the form of a substantially low cylinder comprising an opening, preferably a circular opening, preferably located in the middle of the common base. the inner shell of the inner hollow cylinder is provided in at least one part thereof with a thread compatible with the thread on the outer shell of the sample portion of the holder. In another embodiment, the cap comprises a seal in the space between the inner and outer hollow cylinders. In another embodiment, the base is a set of two low interconnected hollow cylinders of different heights with a common axis of symmetry and a center of the base that is farther from the threaded lid portion. In another embodiment, the opening in the center of the lid is flanked by a hollow cylinder, which may be the outer portion of the liner, the separating member and / or the gasket.

Example 5 Cap Design

An exemplary embodiment of a lid of a device wherein the lid has a regular octagonal prism-shaped upper part compared to the lid of Example 2 (Fig. 5). The diagonal of the octagon is larger than the inner diameter of the container neck.

Example 6 Cap Design

An exemplary embodiment of a lid of a device, wherein the lid is provided with a roughening on the outer surface as compared to Example 4 to facilitate handling (Fig. 6).

Example 7 Sample Part

An exemplary embodiment of a sample portion of the holder, wherein the sample portion has a cylindrical shape at the top with a cylindrical recess (Fig. 7). The bottom is provided with an opening. The outer surface of the cylinder is provided with a thread at its upper edge which can extend to the bottom area with the hole. In some embodiments, the outer casing of the sample portion at the lower edge is knurled or otherwise roughened. In other embodiments, the sample portion on the outer shell is provided with a ring, which may be roughened on its surface, for example in the form of a vertical indentation. Roughening makes handling the device easier. In other embodiments, the bottom of the sample holder portion has protrusions or drain grooves associated with the drain hole.

Example 8 Sample Part

An exemplary embodiment of a sample portion of the holder, wherein the sample portion comprises a recess in the shape of a perpendicular quadrilateral prism at the top of the sample portion of Example 7 (Fig. 8).

Example 9 Sample Part

An exemplary embodiment of a sample portion of the holder, wherein the sample portion includes a recess in the shape of a perpendicular hexagonal prism at the top of the sample portion of Example 7 (Fig. 9).

Example 10 'Sample Part'

ΛΖ

An exemplary embodiment of a sample portion of the holder, wherein the sample portion includes a recess in the upper portion of the trimmed cylinder relative to the sample portion of Example 7 at the top (Fig. 10).

Example 11 Sample Part

An exemplary embodiment of a sample portion of the holder, wherein the sample portion comprises multiple openings in the bottom of the sample portion of Example 7 (Fig. 11).

Example 12 Sample Part

An exemplary embodiment of a sample portion of the holder, wherein the sample portion comprises openings above the bottom (Fig. 12) as opposed to the sample portion of Example 11.

• · · · · ««

- 18 Example 13 Sample Part

An exemplary embodiment of a sample portion of the holder, wherein the sample portion comprises, compared to the sample portion of Example 7, a substantially prism-shaped groove whose height is parallel to the thread axis. The groove facilitates handling of the devices which are inserted into the holder, while at the same time it can serve to prevent the devices from twisting when tightening the lid (Fig. 13).

Example 14 Sample Part

An exemplary embodiment of a sample portion of the holder, wherein the sample portion includes an additional groove compared to the sample portion of Example 13, but does not extend through the entire wall of the sample portion of the holder and together with the first groove serves to prevent the devices inserted into the holder from twisting. ). The grooves in some embodiments extend along the entire length of the recess of the sample portion of the holder.

Example 15 Sample Part

An exemplary embodiment of a sample portion of the holder, wherein the sample portion includes a protrusion over the sample portion of Example 13, which together with the groove serves as a prevention against rotation of the parts inserted into the holder when tightening the lid (Fig. 15).

Example 16 Sample Part

An exemplary embodiment of a sample portion of the holder, wherein the sample portion includes two protrusions, as opposed to the sample portion of Example 13, which serves to prevent the parts inserted into the holder from rotating when the lid is tightened (Fig. 16).

Example 17 Sample Part

An exemplary embodiment of a sample portion of the holder, wherein the sample portion includes a groove relative to the sample portion of Example 8, which facilitates handling of the parts that are inserted into the holder (Fig. 17).

Example 18 Sample Part

An exemplary embodiment of a sample portion of the holder, wherein the sample portion includes a groove relative to the sample portion of Example 9, which facilitates handling of the parts that are inserted into the holder (Fig. 18).

Example 19 Sample Part

An exemplary embodiment of a sample portion of the holder, wherein the sample portion includes a groove relative to the sample portion of Example 10, which facilitates handling of the parts that are inserted into the holder (Fig. 19).

Example 20 Collection Part

An exemplary embodiment of a collection portion of the holder, wherein the collection portion is in the form of a hollow cylinder whose outer diameter is equal to or greater than the diameter of the sample portion of the holder in the bottom region and the inner diameter is greater or equal. t

• * · ¹ • t * such as the diameter of the area where there are holes for draining the solution from the sample portion of the holder. Figure 20 shows a case where the outer diameter is the same as the diameter of the sample portion of the holder in the bottom region.

Example 21 Collection Part

An exemplary embodiment of a collection portion S of a holder, wherein the collection portion S is in the form of a hollow cylinder, the inner diameter of which is greater than or equal to the diameter of the area where the drain holes are at the bottom of the sample portion of the holder Vz and its outer diameter is smaller than the diameter of the sample. parts of the Vz-bracket in the bottom area. Fig. 21 shows the case where the inner diameter of the collecting portion S is the same as the diameter of the drain hole in the bottom of the sample portion of the holder Vz.

Example 22 Collection Part

An exemplary embodiment of a collection portion of the holder wherein the collection portion is in the form of two hollow cylinders. The larger of the rollers has an outer diameter similar to the diameter of the sample portion of the holder in the bottom region. The smaller of the cylinders has an inside diameter equal to or greater than the diameter of the area with drain holes in the bottom of the sample portion. Figure 22 illustrates the case where the outer diameter of the larger of the hollow cylinders is the same as the diameter of the sample portion of the holder in the bottom region and the smaller of the hollow cylinders has an inner diameter equal to the diameter of the drain hole in the bottom of the sample portion of the holder.

Example 23 Holder

An exemplary embodiment of a bracket wherein the bracket is threaded and includes both the sample Vz and the collecting portion S (Fig. 23). The holder also includes a ring positioned around the collecting portion S, which is provided with a roughening for easier handling. The sample portion of the holder Vz corresponds to that shown in FIG. 14, thus comprising two grooves, one of which is through the entire wall of the sample portion Vz and the other which does not extend through the entire wall of the sample portion Vz. Both grooves serve to prevent the devices inserted into the holder from turning when the lid is tightened. The collecting part S is in the form of two hollow cylinders. The outer hollow cylinder has an outer diameter equal to the diameter of the sample portion Vz in the bottom region. The inner hollow cylinder has an inside diameter equal to the diameter of the opening in the bottom of the sample portion Vz.

Example 24 Insert

An embodiment of a liner of a single piece of material, wherein the liner is made in the form of two closely adjacent cylinders through which the opening or openings pass. Fig. 24 shows an insert with a circular hole. The smaller cylinder forms the throat. Alternatively, the insert is formed by only one orifice cylinder or holes, or the lower cylinder is replaced by a plan view in the shape of a polygon, ellipse or circle, which is at least one side delimited by a cutting section. The plate is provided with an opening or openings which form a continuation of the opening in the throat. In some embodiments, the throat is missing and the insert is formed only by a plate having an opening or apertures. In other embodiments, the plate is positioned between the bases of the larger of the rollers. In an alternative embodiment, the liner of Figure 24 is provided on the side of the base of the larger of the rollers, which is:.

directly connects to the smaller cylinder by a hollow cylinder-shaped gasket, through which the neck of the smaller cylinder passes. In another embodiment, the insert of Figure 24 is provided with a hollow-cylinder filter glued from the bottom of the larger base of the larger cylinder. In other embodiments, the insert of Figure 24 is provided with both a gasket and a glue. Alternatively, the orifice (s) in the liner were shaped as a substantially ellipsoidal cylinder, prism or truncated cone or truncated pyramid, or a combination of the shapes, wherein these shapes repeated differently. In other embodiments, the aperture or apertures have been formed substantially as self-joining prisms, truncated cones or truncated pyramids or cylinders of different diameters or cylinders with an ellipse-shaped base of different diameters. In some embodiments, the apertures were formed by a network of tubes connecting the bases of the inner liner. In another embodiment, the inserts described are obtained by gluing the inner and outer portions of the insert.

Example 25 Insert

An exemplary embodiment of a single piece liner, wherein the liner, compared to the liner of Example 24, includes two protrusions (Fig. 25) that engage a groove in the sample portion of the holder of Figs. 14. In another embodiment, the disclosed liner has been obtained by gluing the inner and outer portions of the liner.

Example 26 Insert

An exemplary embodiment of a single piece liner, wherein the liner includes a lower cylinder (Fig. 26) cut from the side opposite the liner of Figure 24. In another embodiment, the disclosed liner has been obtained by gluing the inner and outer portions of the liner.

Example 27 Insert

An exemplary embodiment of a single piece liner, wherein the liner includes two grooves (Fig. 27) relative to the liner of Fig. 24, in which the protrusions in the sample portion of the holder of Figs. 16. In another embodiment, the disclosed liner has been obtained by gluing the inner and outer portions of the liner.

Example 28 Insert

An exemplary embodiment of an insert, wherein the insert includes a groove and a protrusion (Fig. 28) that is compatible with the specimen portion of the holder in Fig. 24. 15. Alternatively, the insert is provided with various combinations of grooves and projections. The grooves are preferably guided along the length of the insert. In another embodiment, the disclosed liner has been obtained by gluing the inner and outer portions of the liner.

Example 29 Outer part of the insert

Example of an outer part of the insert, which is made in the form of a hollow cylinder with two projections (Fig.

29). Alternatively, the projections do not include or comprise grooves or a combination of grooves and projections or the outer portion of the insert is made in the shape of the sides of the lower cylinder of Fig. 26 having an opening for the inner portion of the insert or its planar outer shape.

- 21 - polygon or ellipse. The outer portion of the insert always includes an opening for the inner portion of the insert. Alternatively, the outer part of the insert is a fixed part of the lid. In this embodiment, it is preferably made in the form of a hollow cylinder that flanges the opening in the cap and points vertically to the threaded portion of the cap. The inner and outer parts of the liner may be connected to one another in a non-detachable manner (e.g., made of one piece of material) or in a detachable manner (modularly interlocked when assembling the device). Alternatively, the opening in the outer portion of the liner was essentially a cylinder with an ellipse base, prism or truncated cone or truncated pyramid, or a combination of said shapes. In other embodiments, the opening in the outer portion of the liner has been in the form of substantially adjacent prisms, truncated cones or truncated pyramids or cylinders of different diameters or cylinders with an ellipse-shaped base of different diameters. In other embodiments, the outer portion of the liner of Figure 29 has hollow-cylindrical hollow cylindrical gaskets of the same outer and inner diameters as the diameters of the bases or holes in the bases, respectively, on one or both of the bases.

Example 30 Inner part of the insert

An exemplary embodiment of an inner liner is made in the form of two adjacent cylinders of different diameters. The larger diameter cylinder serves as a thrust element against which the outer portion of the liner of Figure 29 is pressed. The bore extends through the two cylinders. The opening may have different shapes. Figure 30 shows an example where the aperture is cylindrical. Alternatively, the opening or apertures in the inner portion of the liner have been shaped as a substantially ellipsoidal cylinder, prism or truncated cone or truncated pyramid, or a combination of the shapes, wherein these shapes have been repeated in different ways. In other embodiments, the orifice (s) has been shaped substantially as annealing prisms, truncated cones or truncated pyramids or cylinders of different diameters or cylinders with an ellipse-shaped base of different diameters. In some embodiments, the apertures were formed by a network of tubes connecting the bases of the inner liner. Alternatively, the lower cylinder is replaced by a plan in the shape of a polygon, ellipse or circle, which is at least one side bounded by a mowing section. In other embodiments, the lower roller is provided with grooves or projections or a combination thereof. The inner and outer parts of the liner may be connected to one another in a non-detachable manner (e.g., made of one piece of material) or in a detachable manner (modularly interlocked when assembling the device). In other embodiments, the outer shape of the inner portion of the liner is a truncated cone or truncated pyramid or a combination thereof with prisms and cylinders or cylinders with an ellipse-shaped base. Alternatively, the outer shape is a combination of different prisms or cylinders of different diameters, or a combination of cylinders and prisms, or a combination of cylinders, truncated pyramids, truncated cones and prisms with shapes having a circular section or ellipse in plan view. In some embodiments, a hollow cylinder seal or filter having the same external and internal diameters as the diameter of the base and the diameter of the hole in the base has been glued to the base of the inner liner facing the bottom of the sample portion of the holder. In another embodiment, a ring-shaped seal with a diameter of the base was glued onto the base, alternatively the glued seal contained several holes. In other embodiments, the smaller cylinder of Figure 30 was provided with a narrow ring recess at the top or a roughening or thread for

- 22stroke. In another embodiment, the inner portion of the liner was provided with a lid that was detachably squeezed into the opening of the smaller of the cylinders of Figure 30.

Example 31 Inner part of the insert

An exemplary embodiment of an inner liner portion that includes a roughening at the bottom of the liner of Example 30 at the bottom (Fig. 31).

Example 32 Inner part of the insert

An exemplary embodiment of an inner liner portion having four openings as opposed to the inner liner portion of Example 30.

Example 33 Pads

An embodiment of a support, wherein the support is made in the form of a cylinder, which comprises at the top a recess for a sample carrier, such as glass. The recess for the sample carrier is preferably deeper than the thickness of the sample carrier, which avoids cracking of the sample carriers when screwing the device due to pressure of the insert and possibly aids to the support with the sample carrier. The recess is adapted to the shape of the sample carrier, in this embodiment it is cylindrical, because the sample carrier (cover glass) is circular in shape. The recess for the sample carrier may be of any shape, preferably a cylinder or a prism.

At the edge of the recesses for the sample carrier there is a further recess, preferably cylindrical, of smaller diameter than the recess for the sample carrier, which is deeper (extending below the bottom) than the recess for the sample carrier. This smaller recess facilitates removal of the sample carrier from the support. The pad is further provided with a recess for the removal of solvents and / or mixtures, preferably in the form of a cross, which ensures the removal of solvents and / or mixtures. This recess is located on a surface opposite to the surface in which it is recessed for the sample carrier.

In other embodiments, the pad does not include a recess for the sample carrier. In other embodiments, the recess for the sample carrier is substantially cylindrical or frustoconical and may also serve for collecting samples. In other embodiments, the recess for removal of solvents and / or mixtures is provided as a roughening of the underside (opposite to the area in which the recess for the specimen carrier) of the support is provided or the support is provided with ribs in the form of a cross or star-shaped. In other embodiments, the substrate is in the shape of a polygon, ellipse, or circular segment, which ensures compatibility with the internal shape of the sample portion of the holder.

Example 34 Aids - separation parts, filters, seals

Example of separating parts, filters and seals. The filters, seals and separating parts are made in the form of essentially cylinders. In other embodiments, the devices are in the shape of a polygonal, ellipse or circle-shaped plan that is bounded at least on one side by a mower. This ensures their compatibility with the internal shape of the sample holder portion. The filters and seals may be provided with an aperture or apertures that connect their bases. Through the opening or openings that connect their bases,

are always equipped with separation parts. In some embodiments, the separation members are provided with roughening on one or both of the bases.

Example 35 Arrangement of a particle retention device

An exemplary embodiment of an arrangement for trapping particles onto a carrier. The whole assembly consists of the holder D, where the substrate P is located at the bottom of the sample part. The particle support No is placed on the substrate P, on which the filter F_s is placed with a hole of diameter equal to the opening On the filter F is placed the inner part of the VN insert, on which the outer part of the VV insert is slid. Optionally a gasket T is placed on the upper part of the outer part of the VV insert. All aids are placed in the sample part of the holder D and closed with the lid V (Fig. 34). Figure 34 shows a diagram suitable for trapping particles onto a carrier No, eg, round glass. In some embodiments, seal T is used instead of filter F. In replacement of filter F with seal T, centrifugation does not drain solvents and / or mixtures into vessel N. In other embodiments, washer P_a carrier No is omitted, instead of filter F with an aperture F without hole. This filter F is then used to trap particles.

Example 36 Arrangement of a device for washing samples captured on a support

An exemplary embodiment of an apparatus for washing samples captured by carriers. It is possible to use the same scheme as in Fig. 34, or it is possible to insert a seal under the filter, eventually gradually from below the seal and a separating part with an opening. These arrangements provide protection against drying. Another possibility is to use a roughened separating part on the upper side, which ensures faster removal of solvents and / or mixtures. The filter can be completely removed to further speed up the washing.

Example 36 Arrangement of a Filtration Device

An exemplary embodiment of a filtering device arrangement. In contrast to the arrangement of the device of Figure 34, either a filter without an aperture is placed directly on the bottom of the sample portion of the holder, or up to the separation portion, followed by the inner portion of the insert.

Example 37 Arrangement of a device for collecting suspended particles

An exemplary embodiment of an arrangement for collecting suspended particles. In contrast to the arrangement of the apparatus of Figure 34, the pad is provided with a recess for retaining the particles and the particle carrier is not incorporated into the assembly.

Example 38 Stand

Example of stand construction. The stand is made in the form of a base and darkness (Fig. 35) in the shape of a substantially cylinder having a smaller diameter than the hole diameter in the sample portion of the holder. The insertion of the device according to the invention onto the stand facilitates the removal of the insert and possible aids from the holder.

• · · · · · · · · · · · · · · · · · · · · · ·

t t t t t

Example 39 An exemplary embodiment of the apparatus substantially according to the arrangement of Figure 34 for containers having an inner neck diameter of 22 mm to 44 mm

An embodiment of the apparatus of the present invention essentially according to the arrangement of Figure 34 and other variants thereof.

The lid was made in an arrangement that substantially corresponded to that of FIG. 6. The lid was made of polytetrafluoroethylene. The overall lid height was alternatively 10, 20, 30, 40 or 60 mm. The height of the two hollow cylinders was alternatively 5, 15, 18, 35 or 55 mm. Alternatively, a cap with a hollow cylinder arrangement was made which substantially corresponded to that of FIG. In this case, the height of the inner hollow cylinder was alternatively 5, 15, 18, 35 or 55 mm and the height of the outer hollow cylinder was alternatively 5, 15, 35 or 55 mm. The inner diameter of the inner hollow cylinder was alternatively 18, 21, 22, 24 or 32 mm. In the outer hollow cylinder, the inner diameter was alternatively 30, 32, 33, 34, 38 or 44 mm. The outer diameter of the inner hollow cylinder was alternatively 22, 26, 26.5, 29 or 37 mm, the outer diameter of the outer hollow cylinder was alternatively 33, 38, 47 or 57 mm. Alternatively, the lid was manufactured without the outer cylinder substantially in the configuration of Fig. 2. In this case, the portion of the lid forming the base of the hollow cylinder with the central hole in the middle had an alternatively 33, 38, 47 or 57 mm diameter. Alternatively, the circular aperture in the lid of all arrangements had a diameter of 4.2, 7.4, 12.5, 12.7, 12.8, 14, or 23 mm. Depending on the arrangement, the internal hollow cylinder or the hollow cylinder was threaded around the hole. In some embodiments, the outer hollow cylinder has also been threaded. The thread depth was alternatively 0.2, 1, 2 or 3 mm. The thread was either only in part or along the entire inner shell of the threaded hollow cylinders.

The holder substantially in the configuration of FIG. 23 was made of polytetrafluoroethylene. The total length of the holder was alternatively 11, 42, 53.5, 55 or 92 mm. The recess depth of the sample holder portion was alternatively 6,

12.5, 13, 14, 16, 36 or 82mm. The diameter of the recess of the sample portion was alternatively 11, 18 or 24 mm. The wall groove width was alternatively 1, 2, 4 or 6 or 10 mm. The grooves extended to close proximity to the bottom, or were alternatively terminated at 0.5, 1, 1.5, 2, 4 or 5 mm above the bottom. The diameter of the hole in the bottom of the sample portion of the holder was alternatively 2, 10, 15 or 22 mm. Alternatively, 4, 8 or 12 holes were made in the bottom, alternatively 1 or 2 mm in diameter. Brackets have also been made where the apertures have been placed exclusively in the recess wall above the bottom substantially according to the arrangement of FIG. 12. The height of the bottom edge of the holes above the bottom of the sample portion of the holder was alternatively 2, 10, 15 or 20 mm above the bottom. The ring diameter was alternatively 20.1, 25.5, 28.1 or 35 mm. Some of the holders did not contain grooves or contained only one groove. Others contained 3 or 4 grooves.

The outer part of the insert was made essentially in the configuration of Fig. 29 from polytetrafluoroethylene. If the outer portion of the insert was provided with protrusions, its outer diameter was alternatively 0.05, 0.1, 0.2, 0.4 or 0.8 mm smaller than the diameter of the cylindrical recess in the sample portion of the holder. The hole in the outer part of the insert was alternatively 8, 12 or 20 mm in diameter. The length of the outer part of the insert was alternatively 5 (2,

8.5, 12.5, 15, 32.5 or 80 mm. In some cases, the prisms in the form of essentially prisms were guided only over part of the sheath or were completely missing.

• · · ·

The inner portion of the liner substantially in the configuration of Fig. 30 was made of polytetrafluoroethylene. The overall height of the inner liner was alternatively 9, 16, 21, 39 or 90 mm. The diameter of the larger diameter cylinder was the same as the outer diameter of the liner. Alternatively, the height of the larger diameter cylinder was 0.5, 2, 4.2, 10, or 30 mm. Alternatively, the orifice was formed in a shape with a portion formed by a cylinder and a portion by a truncated cone. The larger base of the cone fully overlapped with the base of the cylinder, resulting in a continuous narrowing of the opening. The truncated cone resulted in a side of the inner part of the liner which was made in the form of a cylinder of larger diameter. This orifice had alternatively a diameter of 2, 4.2, 7.5, 9 or 14 mm. In another variation, the hole was made in the form of a cylinder with a diameter of 7.5 mm on the side of the larger cylinder, followed by a cylinder with a diameter of 0.05 or 0.1 mm, followed by a truncated cone whose smaller base had a diameter as smaller the cylinder and the top base had a diameter of 10 mm. The inner portions of the insert having several holes were also made. Alternatively, inner liners were provided that were provided with 1, 2, 3 or 4 protrusions on the larger diameter cylinder shell.

The pad substantially according to the arrangement of FIG. 33 was made of polytetrafluoroethylene. Its diameter was the same as the diameter of the cylinder with the larger diameter of the inner liner. The depth of the larger recess was alternatively 0.1, 0.2, 0.3, 0.4, 0.9 or 5 mm. Alternatively, the depth of the smaller recess was 0.1, 0.2, 0.3, 0.4, or 0.5 mm larger than the depth of the larger recess. The diameter of the larger recess was alternatively 5.5, 10.5,

12.5 or 14.5 mm. The drain channels alternatively had a depth of 0.1, 0.5, 1, or 1.5 mm.

Alternatively, the apparatus comprised a low roller support made of 0.3 mm high paper having a diameter equal to the diameter of the drain channel support.

The filter was made as an annulus of filter paper. Its outer diameter was the same as that of the washer, and the inner diameter was the same as the diameter of the orifice in the inner portion of the liner in the higher diameter cylinder. A filter without a hole was also made.

The gasket located above the outer liner that enclosed the neck of the inner liner was made in the form of a hollow cylinder of silicone rubber and had a maximum outer diameter of 0.05, 0.1, 0.2, 0.5, 1 or 3 mm smaller than the diameter of the cylindrical recess of the sample holder portion and the inner diameter alternatively 0.05, 0.1, 0.2, or 0.4 mm larger than the outer diameter of the smaller cylinder of the inner liner. The seal height was alternatively 0.5, 3, 10 or 20 mm.

In some cases, the apparatus has included a separation piece or several separation pieces in the form of hollow cylinders made of polytetrafluoroethylene. Their outside diameter was the same as that of the washer. The inner diameter was the same as the diameter of the orifice in the cylinder of the larger diameter inner liner. Their height was alternatively 0.2, 0.5, 3, 5, 10 or 20 mm.

The device in some embodiments included another seal, which was again made in the form of a hollow cylinder of silicone rubber. Its outside diameter was the same as that of the washer. The inner diameter was the same as the diameter of the orifice in the inner portion of the liner in a larger diameter cylinder. Its height was alternatively 0.2, 0.5, 3, 5, 10 or 20 mm. This seal served either as a separator with a sealing function when it was inserted between the sample carrier and the filter or was used between the separator and the sample carrier.

«·

- 26 Another seal has been manufactured for situations where the equipment has been used for vacuum pump processing. This seal was inserted between the inner and outer part of the insert. Its outer diameter was equal to or greater than the outer diameter of the liner. Alternatively, its inner diameter was 0.05, 0.1, 0.2, or 0.4 mm larger than the outer diameter of the smaller cylinder of the inner liner.

In some cases, the device included a truncated cone-shaped pad. The height and other characteristics were the same as the two-cylinder recess.

In some embodiments, the lid around the opening from the side facing the threaded lid portion has been provided with a hollow cylinder having dimensions that were the same as the cylindrical portions of the outer portion of the liner or separator member. In these cases, the recess in the sample portion of the holder had the shape of a cylinder with a larger diameter than the diameter of the hollow cylinder around the opening in the lid.

The devices made were suitable for containers with inner neck diameters greater than 22 mm and outer neck diameters less than 44 mm. Polytetrafluoroethylene has been replaced in some embodiments by polyethylene, or polyoxymethylene, or metal alloys. The filters were alternatively made of nitrocellulose, nylon, polyvinylidene difluoride, glass microfibers, polypropylene, polysulfone, cellulose acetate or polytetrafluoroethylene. Depending on their configuration, the devices produced are suitable both for sedimentation or filtration, and for the processing of samples on supports by centrifugal or suction forces.

Industrial applicability

The device allows for a firm grip in vessels with a hollow-cylindrical neck and, in conjunction with aids, allows various solvent and mixture operations and sample operations on carriers mainly by centrifugal or gravity force or by using suction force by means of vacuum pumps. Solvents and / or mixtures may be simultaneously collected in containers. Examples of operations are filtration of solvents and / or mixtures, sedimentation of particles contained in solvents and / or mixtures, simultaneous filtration and sedimentation, washing of sample particles or samples adhered to substrates.

Claims (17)

A device for facilitating the handling of solvents, mixtures and samples on carriers, characterized in that it comprises a holder, an insert, a lid and at least one aid, wherein: the holder comprises a sample portion, substantially cylindrically provided with a recess for receiving the insert, the lower portion of the recess having at least one opening, and wherein the outer shell of the sample portion of the holder is threaded in at least one portion thereof, the outer shape of the holder substantially a cylinder; the liner consists of an outer portion and an inner portion, the outer portion of the liner having an opening for the inner portion of the liner connecting opposing bases of the outer portion of the liner, the inner portion of the liner having at least one opening connecting its opposing bases; the size and shape of the recess of the sample holder portion and the outer shape of the inner liner are at least in part of the inner liner adapted to the opening in the outer liner and at the same time at least in the inner liner larger than the narrowest point of the opening in the outer liner; - the lid comprises a substantially cylindrical recess, the inner lid of the lid recess having at least one portion thereof having a thread compatible with the thread on the outer shell of the sample portion of the holder, and wherein the lid portion opposite the lid portion containing the recess is provided with at least one hole; preferably circular; wherein the portion of the cap around the aperture or apertures in the cap in the region facing the recess of the cap is at least in some of its part larger than the plan view of the outer portion of the insert and / or the devices disposed in the device; the device is shaped and sized to be inserted into a sample portion of the holder, and is selected from the group consisting of filters, washers, seals, separators, sample carriers, or combinations thereof. Device according to claim 1, characterized in that the inner and outer parts of the insert are connected to one another in a non-detachable or detachable manner. The apparatus of claim 1 or 2, wherein the holder further comprises a collecting portion having the shape of a hollow cylinder or two interposed hollow cylinders, wherein the hollow cylinder or the inner of the two interposed hollow cylinders has the same or larger diameter than the diameter of the opening in the base of the sample portion of the holder, preferably this diameter is the same as the diameter of the opening in the base of the sample portion of the holder, the collecting portion of the holder being connected to the lower base of the sample portion of the holder. Apparatus according to any one of the preceding claims, characterized in that the lid is in the form of substantially two hollow cylinders of the same or different heights, the two hollow cylinders being provided with one common base comprising an opening, preferably a circular opening, preferably located. in the middle of the common base, and wherein the inner shell of the inner hollow cylinder is provided with at least one of its parts with a thread compatible with the thread on the outer shell of the sample portion of the holder. 4 · Device according to any one of the preceding claims, characterized in that the outer surface of the lid and / or the outer surface of the holder are provided with roughening. Device according to any one of the preceding claims, characterized in that the recess of the sample portion of the holder has a substantially shape selected from the group comprising a cylinder, a prism, preferably a perpendicular prism, an elliptical cylinder, a shape derived from a cylinder whose plan view is a circular section. Device according to any one of the preceding claims, characterized in that the inner wall of the sample portion of the holder is provided with at least one groove and / or projection, preferably in the form of a substantially prism or prism with rounded edges with a height parallel to the thread axis. Device according to claim 7, characterized in that the groove extends through the entire wall of the sample portion of the holder. Apparatus according to any one of the preceding claims, characterized in that the outer diameter of the inner liner is less than the diameter of the opening in the lid, wherein the height of the inner liner passing through the opening of the outer liner is greater than the height of the opening in the outer liner. Device according to any one of the preceding claims, characterized in that it comprises a holder in the sample portion of which a recess with a recess for the sample carrier or a recess without a recess is located, a sample carrier which is preferably glass over which a filter is pressed. of the holder above the filter placed by the insert and optionally above the gasket, the neck of the inner part of the insert being threaded through a hole in the cap screwed to the holder by means of a thread. 11. Apparatus according to claim 10, further comprising a seal situated between the sample carrier and the filter, optionally a seal and a separating member between the sample carrier and the filter, and preferably a seal is inserted between the inner and outer portions of the insert. Device according to any one of the preceding claims, characterized in that the at least one device is permanently attached to the insert, preferably the device being a seal and / or a filter. Apparatus according to any one of the preceding claims, characterized in that the at least one device is releasably connected to the lid, preferably the device is a seal and / or a separating member. Device according to any one of the preceding claims, characterized in that the outer part of the insert and the lid are non-detachably connected. · « - 29 - A kit for handling solvents, mixtures and samples on carriers, characterized in that it comprises a device for facilitating handling of solvents, mixtures and samples on carriers according to any one of the preceding claims, and a container, preferably a container with a hollow-cylinder neck. 16. The kit of claim 15, further comprising a pedestal including a base and a mandrel adapted to be inserted into an opening at the bottom of the sample portion of the holder, optionally through an opening in the collection portion of the holder. Use of a device for facilitating the handling of solvents, mixtures and samples on carriers according to any one of claims 1 to 14 and / or a kit according to claims 15 or 16 for filtering mixtures, sedimenting particles, washing particles and samples on carriers, in particular using centrifugal forces for centrifugation and / or suction forces mediated by vacuum pumps. • · Annotation — Title — Device for facilitating the handling of samples and samples on carriers. The present invention provides a device for facilitating the handling of solvents, compositions and samples on carriers. The article includes a holder, a lid, an insert, and at least one a device, where: the holder comprises a sample portion, substantially cylindrically provided with a recess for receiving the insert, the lower portion of the recess having at least one opening, and wherein the outer shell of the sample portion of the holder is threaded in at least one portion thereof, the outer shape of the holder substantially a cylinder; the liner consists of an outer portion and an inner portion, the outer portion of the liner having an opening for the inner portion of the liner connecting opposing bases of the outer portion of the liner, the inner portion of the liner having at least one opening connecting its opposing bases; the size and shape of the recess of the sample holder portion and the outer shape of the inner liner are at least in part of the inner liner adapted to the opening in the outer liner and at the same time at least in the inner liner larger than the narrowest point of the opening in the outer liner; - the lid comprises a substantially cylindrical recess, the inner lid of the lid recess having at least one portion thereof having a thread compatible with the thread on the outer shell of the sample portion of the holder, and wherein the lid portion opposite the lid portion containing the recess is provided with at least one hole; preferably circular; wherein the portion of the cap around the aperture or apertures in the cap in the region facing the recess of the cap is at least in some of its part larger than the plan view of the outer portion of the insert and / or the devices disposed in the device; the device is shaped and sized to be inserted into a sample portion of the holder, and is selected from the group consisting of filters, washers, seals, separators, sample carriers, or combinations thereof. The present invention relates to a kit for the use of solvents and mixtures thereof in carriers and their use.