[go: up one dir, main page]

CY1110695T1 - Μεθοδοι για την δημιουργια υψηλου τιτλου ανευ-βοηθου παρασκευασματων απελευθερωμενων ανασυνδυασμενων aav φορεων - Google Patents

Μεθοδοι για την δημιουργια υψηλου τιτλου ανευ-βοηθου παρασκευασματων απελευθερωμενων ανασυνδυασμενων aav φορεων

Info

Publication number
CY1110695T1
CY1110695T1 CY20091100126T CY091100126T CY1110695T1 CY 1110695 T1 CY1110695 T1 CY 1110695T1 CY 20091100126 T CY20091100126 T CY 20091100126T CY 091100126 T CY091100126 T CY 091100126T CY 1110695 T1 CY1110695 T1 CY 1110695T1
Authority
CY
Cyprus
Prior art keywords
headlift
preparatory
formats
aid
creating
Prior art date
Application number
CY20091100126T
Other languages
English (en)
Inventor
Edward Morrow Atkinson
Victor P Fung
Perry C Wilkins
Ryan K Takeya
Thomas C Reynolds
Original Assignee
Targeted Genetics Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Targeted Genetics Corporation filed Critical Targeted Genetics Corporation
Publication of CY1110695T1 publication Critical patent/CY1110695T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C12N2750/14152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Η εφεύρεση που περιγράφεται εδώ παρέχει μεθόδους παραγωγής rAAV σωματιδίων με καλλιέργεια κυττάρων παραγωγών υπό συνθήκες, όπως θερμοκρασία και pΗ, που προάγουν απελευθέρωση ιού.
CY20091100126T 1998-09-04 2009-02-04 Μεθοδοι για την δημιουργια υψηλου τιτλου ανευ-βοηθου παρασκευασματων απελευθερωμενων ανασυνδυασμενων aav φορεων CY1110695T1 (el)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14247498A 1998-09-04 1998-09-04
US12368599P 1999-03-10 1999-03-10
EP99968678A EP1109892B1 (en) 1998-09-04 1999-09-07 Methods for generating high titer helper-free preparations of released recombinant aav vectors

Publications (1)

Publication Number Publication Date
CY1110695T1 true CY1110695T1 (el) 2015-06-10

Family

ID=26821785

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20091100126T CY1110695T1 (el) 1998-09-04 2009-02-04 Μεθοδοι για την δημιουργια υψηλου τιτλου ανευ-βοηθου παρασκευασματων απελευθερωμενων ανασυνδυασμενων aav φορεων

Country Status (10)

Country Link
EP (1) EP1109892B1 (el)
JP (1) JP4472182B2 (el)
AT (1) ATE414146T1 (el)
CA (1) CA2342849C (el)
CY (1) CY1110695T1 (el)
DE (1) DE69939903D1 (el)
DK (1) DK1109892T3 (el)
ES (2) ES2541540T3 (el)
PT (2) PT1109892E (el)
WO (1) WO2000014205A2 (el)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6924128B2 (en) 1994-12-06 2005-08-02 Targeted Genetics Corporation Packaging cell lines for generation of high titers of recombinant AAV vectors
US6995006B2 (en) 1997-09-05 2006-02-07 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of released recombinant AAV vectors
US6566118B1 (en) 1997-09-05 2003-05-20 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of released recombinant AAV vectors
CA2682108C (en) * 1997-09-05 2013-12-24 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of recombinant aav vectors
US6346415B1 (en) 1997-10-21 2002-02-12 Targeted Genetics Corporation Transcriptionally-activated AAV inverted terminal repeats (ITRS) for use with recombinant AAV vectors
US6893865B1 (en) 1999-04-28 2005-05-17 Targeted Genetics Corporation Methods, compositions, and cells for encapsidating recombinant vectors in AAV particles
DK1180159T3 (da) 1999-05-28 2008-11-17 Targeted Genetics Corp Fremgangsmåder og sammensætninger til at sænke niveauet af Tumor-Nekrose-Faktor (TNF) i TNF-associerede lidelser
EP1939300A1 (en) 1999-05-28 2008-07-02 Targeted Genetics Corporation Methods and compositions for lowering the level of tumor necrosis factor (TNF) in TNF-associated disorders
JP4827353B2 (ja) 1999-08-09 2011-11-30 ターゲティッド ジェネティクス コーポレイション 鎖内塩基対を形成するような配列の設計による、組換えウイルスベクターからの一本鎖の異種ヌクレオチド配列の発現の増大
US7115391B1 (en) 1999-10-01 2006-10-03 Genovo, Inc. Production of recombinant AAV using adenovirus comprising AAV rep/cap genes
CN101065479A (zh) * 2004-10-15 2007-10-31 蒙奈尔化学感觉中心 培养哺乳动物味细胞的方法
AU2005304866B2 (en) * 2004-11-05 2010-04-01 Wellstat Biologics Corporation Stable and filterable enveloped virus formulations
EP3988660B1 (en) 2009-05-02 2025-05-14 Genzyme Corporation Gene therapy for neurodegenerative disorders
WO2012122625A1 (en) * 2011-03-14 2012-09-20 National Research Council Of Canada Method of viral production in cells
JP5861001B2 (ja) 2013-07-11 2016-02-16 タカラバイオ株式会社 非エンベロープウイルスの製造方法
US10023846B2 (en) 2014-07-10 2018-07-17 Takara Bio Inc. Production method for non-enveloped virus particles
KR102493350B1 (ko) 2015-01-09 2023-01-27 다카라 바이오 가부시키가이샤 비엔벨로프 바이러스 입자의 제조방법
KR102450255B1 (ko) * 2016-05-25 2022-10-04 론자 휴스턴 아이엔씨. 폴리디알릴 디알킬암모늄 염을 이용한 아데노-관련 바이러스의 분리방법
US11795473B2 (en) 2016-10-14 2023-10-24 Ultragenyx Pharmaceutical Inc. Use of tonicifying agents to enhance recombinant adeno-associated virus yield
WO2023114901A2 (en) * 2021-12-15 2023-06-22 Oxford Biomedica Solutions Llc Methods and compositions for the production of adeno-associated virus
KR20250162543A (ko) 2023-03-21 2025-11-18 에프. 호프만-라 로슈 아게 재조합 aav 입자 제제 생산 방법
WO2025168663A1 (en) 2024-02-09 2025-08-14 F. Hoffmann-La Roche Ag Method for producing recombinant adeno-associated viral particles

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6686200B1 (en) * 1993-08-31 2004-02-03 Uab Research Foundation Methods and compositions for the large scale production of recombinant adeno-associated virus
WO1997008298A1 (en) * 1995-08-30 1997-03-06 Genzyme Corporation Chromatographic purification of adenovirus and aav
US5910434A (en) * 1995-12-15 1999-06-08 Systemix, Inc. Method for obtaining retroviral packaging cell lines producing high transducing efficiency retroviral supernatant
FR2750433B1 (fr) * 1996-07-01 1998-08-14 Rhone Poulenc Rorer Sa Procede de production d'adenovirus recombinants
CA2682108C (en) * 1997-09-05 2013-12-24 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of recombinant aav vectors

Also Published As

Publication number Publication date
CA2342849C (en) 2013-01-29
JP2002524070A (ja) 2002-08-06
ES2317709T3 (es) 2009-04-16
EP1109892B1 (en) 2008-11-12
PT1109892E (pt) 2009-02-17
PT1930418E (pt) 2015-08-26
DE69939903D1 (de) 2008-12-24
EP1109892A2 (en) 2001-06-27
WO2000014205A3 (en) 2000-05-25
ES2541540T3 (es) 2015-07-21
DK1109892T3 (da) 2009-02-09
WO2000014205A2 (en) 2000-03-16
CA2342849A1 (en) 2000-03-16
ATE414146T1 (de) 2008-11-15
JP4472182B2 (ja) 2010-06-02
WO2000014205A9 (en) 2000-09-08

Similar Documents

Publication Publication Date Title
CY1110695T1 (el) Μεθοδοι για την δημιουργια υψηλου τιτλου ανευ-βοηθου παρασκευασματων απελευθερωμενων ανασυνδυασμενων aav φορεων
AU2002255511A1 (en) Charge tags and separation of nucleic acid molecules
DE69934242D1 (de) Polyketid synthase enzyme und rekombinante dna konstrukte dafür, zur herstellung von mit fk-506 und fk-520 verwandten verbindungen
TWI315740B (el)
NZ532665A (en) Neisseria meningitidis antigens and compositions
EP1126872A4 (en) IMMUNOTHERAPY AND DIAGNOSIS OF CANCER USING UNIVERSAL ANTIGENS ASSOCIATED WITH TUMORS SUCH AS HTERT
PL344475A1 (en) Bridged indenopyrrolocarbazoles
YU5800A (sh) Univerzalno primenljiva krvna plazma
GB0031527D0 (en) New use
WO2001092473A3 (de) Verfahren zur kultivierung von knorpelersatz und biomatrix nach diesem verfahren hergestellt
EA200600459A1 (ru) Способ получения цитотоксических лимфоцитов
ATE555194T1 (de) Methode zur herstellung von proliferierenden und differenzierenden zelllinien
ATE286073T1 (de) Antikörper gegen spaltprodukte von vimentin
DK0896627T3 (da) Indvinding af natamycin
DK0787124T3 (da) Fremgangsmåde til fremstilling af substituerede 3-aminobenzonitriler
CY1116418T1 (el) Διεργασια για την παραγωγη r-(+)-6-καρβοξαμιδο-3-ν-μεθυλαμινο-1,2,3,4-τετραϋδροκαρβαζολιου
NO970030L (no) Isolert nukleinsyresekvens som koder for et tumor-rejeksjonsantigen presentert av HLA-A2
DK0931161T3 (da) Fremgangsmåde til biotransformation af colchicinoid-forbindelser til de tilsvarende 3-glycosylderivater
AU2001278700A1 (en) Method of culturing human chondrocytes
AR022150A1 (es) Acidos nucleicos derivados de monocitos y composiciones y metodos relacionados
AU3189199A (en) Antisense modulation of lfa-3
DK1086114T3 (da) Morfin-6-glucuronidsyntese
YU1500A (sh) Postupak za dobijanje aminskih derivata alkiloksifuranona, dobijena jedinjenja i korišćenje ovih jedinjenja
CY1116753T1 (el) Μεθοδοι για την παραγωγη παρασκευασματων υψηλου τιτλου χωρις βοηθους των αποδεσμευμενων ανασυνδυασμενων φορεων aav
ATE522604T1 (de) Verfahren zur herstellung von proteinen durch elektropulsierung