[go: up one dir, main page]

CY1110438T1 - Χιμαιρικες (πρωτεϊνες συντηξης) gfp-ακουορινης ως βιοφωταυγεις αναφορεις ca++ στο επιπεδο του ενος κυτταρου - Google Patents

Χιμαιρικες (πρωτεϊνες συντηξης) gfp-ακουορινης ως βιοφωταυγεις αναφορεις ca++ στο επιπεδο του ενος κυτταρου

Info

Publication number
CY1110438T1
CY1110438T1 CY20081101381T CY081101381T CY1110438T1 CY 1110438 T1 CY1110438 T1 CY 1110438T1 CY 20081101381 T CY20081101381 T CY 20081101381T CY 081101381 T CY081101381 T CY 081101381T CY 1110438 T1 CY1110438 T1 CY 1110438T1
Authority
CY
Cyprus
Prior art keywords
bioslighters
aquorine
gfp
references
chemical
Prior art date
Application number
CY20081101381T
Other languages
English (en)
Inventor
Valérie Baubet
Mouellic Hervé Le
Philippe Brulet
Original Assignee
Institut Pasteur
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Pasteur filed Critical Institut Pasteur
Publication of CY1110438T1 publication Critical patent/CY1110438T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00—Peptide or protein sequence
    • Y10S930/01—Peptide or protein sequence
    • Y10S930/31—Linker sequence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Ένα τροποποιημένο βιοφωταυγές σύστημα που περιέχει ένα φθορίζον μόριο (που είναι) συνδεμένο ομοιοπολικά με μία φωτοπρωτεΐνη, όπου η εν λόγω σύνδεση μεταξύ των δύο πρωτεϊνών έχει τη λειτουργία να σταθεροποιεί το τροποποιημένο βιοφωταυγές σύστημα και επιτρέποντας τη μεταφορά της ενέργειας μέσω Μεταφοράς Ενέργειας Συντονισμού Χημειοφωταύγειας (CRET).
CY20081101381T 2000-06-01 2008-12-02 Χιμαιρικες (πρωτεϊνες συντηξης) gfp-ακουορινης ως βιοφωταυγεις αναφορεις ca++ στο επιπεδο του ενος κυτταρου CY1110438T1 (el)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US20831400P 2000-06-01 2000-06-01
US21052600P 2000-06-09 2000-06-09
US25511100P 2000-12-14 2000-12-14
EP01949426A EP1292613B9 (en) 2000-06-01 2001-06-01 Chimeric GPF-Aequorin as bioluminiscent Ca++ reporters at the single cell level

Publications (1)

Publication Number Publication Date
CY1110438T1 true CY1110438T1 (el) 2015-04-29

Family

ID=27395180

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20081101381T CY1110438T1 (el) 2000-06-01 2008-12-02 Χιμαιρικες (πρωτεϊνες συντηξης) gfp-ακουορινης ως βιοφωταυγεις αναφορεις ca++ στο επιπεδο του ενος κυτταρου

Country Status (13)

Country Link
US (3) US6936475B2 (el)
EP (1) EP1292613B9 (el)
JP (1) JP5389307B2 (el)
CN (1) CN100467604C (el)
AT (1) ATE407142T1 (el)
AU (1) AU784887B2 (el)
CA (1) CA2411304C (el)
CY (1) CY1110438T1 (el)
DE (1) DE60135652D1 (el)
DK (1) DK1292613T3 (el)
ES (1) ES2312454T3 (el)
PT (1) PT1292613E (el)
WO (1) WO2001092300A2 (el)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU784887B2 (en) * 2000-06-01 2006-07-20 Centre National De La Recherche Scientifique Chimeric GFP-aequorin as bioluminescent CA++ reporters at the single cell level
JP2004508042A (ja) * 2000-09-07 2004-03-18 オウマン，クリスター，エス．，オー． 細胞表面受容体−リガンド結合のためのレポーターシステム
DE03779067T1 (de) * 2002-11-12 2006-06-22 Zakrytoe Aktsionernoe Obschestvo "Evrogen" Fluoreszenzproteine und chromoproteine aus nicht-aequorea-hydrozoa-spezies sowie verfahren zur verwendung davon
CN1304571C (zh) * 2003-10-16 2007-03-14 清华大学 一种培育转基因斑马鱼的方法及其专用质粒
US8263821B2 (en) 2004-02-12 2012-09-11 Institut Pasteur Non-invasive real-time in vivo bioluminescence imaging of local CaÂ2+ dynamics in living organisms
GB0625678D0 (en) * 2006-12-21 2007-01-31 Lux Biotechnology Ltd Composition and method for detection of demineralisation
ES2427854B1 (es) * 2012-03-29 2014-09-15 Universidad De Valladolid Sensores de calcio y métodos para la detección de calcio libre intracelular
ES2452485B1 (es) * 2012-07-13 2015-03-09 Univ Valladolid Mutantes de apoacuorina y metodos para su uso
ES2445018B1 (es) * 2012-07-27 2014-12-12 Universidad De Castilla La Mancha VARIANTES DE LA PROTEÍNA DE FUSIÓN SENSIBLE AL CALCIO tdTOMATO-AEQUORINA
FR2996225B1 (fr) 2012-10-03 2014-10-03 Centre Nat Rech Scient Analogues de la coelenterazine emettant une lumiere decalee vers le rouge visant l'imagerie in vivo
GB201700317D0 (en) 2017-01-09 2017-02-22 Calcivis Ltd Detection device
US11013411B1 (en) 2018-06-13 2021-05-25 The Research Foundation For The State University Of New York Chromatic bioluminescence as a cellular level readout system of neural activity
USD1042180S1 (en) 2020-05-28 2024-09-17 Calcivis Limited Dental detection device

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8916806D0 (en) * 1989-07-22 1989-09-06 Univ Wales Medicine Modified proteins
US5498521A (en) * 1990-01-24 1996-03-12 President And Fellows Of Harvard College Diagnosis of hereditary retinal degenerative diseases
US5614184A (en) * 1992-07-28 1997-03-25 New England Deaconess Hospital Recombinant human erythropoietin mutants and therapeutic methods employing them
US5714666A (en) * 1993-02-09 1998-02-03 Children's Hospital Of Philadelphia Measurement of intracellular calcium using bioluminescent apoaequorin expressed in mammalian cells
US6818439B1 (en) * 1994-12-30 2004-11-16 Chiron Corporation Methods for administration of recombinant gene delivery vehicles for treatment of hemophilia and other disorders
US5744326A (en) * 1996-03-11 1998-04-28 The Immune Response Corporation Use of viral CIS-acting post-transcriptional regulatory sequences to increase expression of intronless genes containing near-consensus splice sites
DE69938293T2 (de) * 1998-03-27 2009-03-12 Bruce J. Beverly Hills Bryan Luciferase, gfp fluoreszenzproteine, kodierende nukleinsaüre und ihre verwendung in der diagnose
ATE248226T1 (de) * 1998-04-17 2003-09-15 Rigel Pharmaceuticals Inc Multiparameter facs test zum ermitteln von änderungen in den zellularen parametern
US6800492B2 (en) 2000-06-01 2004-10-05 Institute Pasteur Chimeric GFP-aequorin as bioluminescent Ca++ reporters at the single cell level
AU784887B2 (en) * 2000-06-01 2006-07-20 Centre National De La Recherche Scientifique Chimeric GFP-aequorin as bioluminescent CA++ reporters at the single cell level

Also Published As

Publication number Publication date
AU7058301A (en) 2001-12-11
PT1292613E (pt) 2008-11-10
WO2001092300A9 (en) 2009-01-22
JP2004532001A (ja) 2004-10-21
ATE407142T1 (de) 2008-09-15
US6936475B2 (en) 2005-08-30
US20080213879A1 (en) 2008-09-04
JP5389307B2 (ja) 2014-01-15
CN1468312A (zh) 2004-01-14
ES2312454T3 (es) 2009-03-01
CN100467604C (zh) 2009-03-11
EP1292613A2 (en) 2003-03-19
WO2001092300A2 (en) 2001-12-06
US20100330667A1 (en) 2010-12-30
WO2001092300A3 (en) 2002-11-07
EP1292613B1 (en) 2008-09-03
DK1292613T3 (da) 2008-11-24
AU784887B2 (en) 2006-07-20
CA2411304C (en) 2014-07-29
EP1292613B9 (en) 2009-04-01
US20030175807A1 (en) 2003-09-18
CA2411304A1 (en) 2001-12-06
DE60135652D1 (de) 2008-10-16
US7763443B2 (en) 2010-07-27
HK1060370A1 (zh) 2004-08-06

Similar Documents

Publication Publication Date Title
CY1110438T1 (el) Χιμαιρικες (πρωτεϊνες συντηξης) gfp-ακουορινης ως βιοφωταυγεις αναφορεις ca++ στο επιπεδο του ενος κυτταρου
ES2167307T3 (es) Proteinas bioluminiscentes modificadas y su uso.
EA200100773A1 (ru) Замещенные 1-гетероциклические диариламины
ATE301122T1 (de) Kernerweiterte perylenbisimide und ihre verwendung als fluoreszenzfarbstoffe
DE69534235D1 (de) Cytokin lerk-7
DE69837838D1 (de) Organische, elektrolytische batterie
BR0114199A (pt) Sequências de nucleotìdeos que medeiam a fertilidade masculina e métodos de uso das mesmas
DE60228152D1 (de) Brennstoffzellensystem
CO5550468A2 (es) Inmunoconjugados del anticuerpo cd44 citotoxico
ATE541857T1 (de) Optimierte fc-varianten und herstellungsverfahren dafür
ES2130232T3 (es) N,n'-diacilhidrazinas n' sustituidas como insecticidas.
WO2004019861A3 (en) Stable ph optimized formulation of a modified antibody
DE58907885D1 (de) Benzimidazol-2-carbonsäureanilide, ihre Verwendung als Lichtschutzmittel für organisches Material und mit diesen Aniliden stabilisiertes organisches Material.
NL1021701A1 (nl) PEM-brandstofcel.
ATE284407T1 (de) Pyrimidinderivate als markierte bindungspartner
BR0115121A (pt) Compostos n-((1,2,4)triazoloazinil)tiofenossulfonamida como herbicidas
FI961581L (fi) Elektroditon lamppu, jonka tehokkuutta on parannettu
EA200200091A1 (ru) Содержащая амброксол пастилка
BR0007922A (pt) Compostos calcilìticos
SG155923A1 (en) Fluorescent dye compounds, conjugates and uses thereof
SE8604991D0 (sv) Snake venom growth arresting peptide
DE50114447D1 (de) Brennstoffzellensystem mit zugeordnetem Gaserzeugungssystem
EA200200798A1 (ru) Кальцилитические соединения
ES2188273T3 (es) Farmacos anticarcinogenos a base de antraquinona.
AR020759A1 (es) Proceso de oxidacion selectiva y catalizador para el mismo