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CU22387A1 - Monoclonal anti-bodies which recognize the epidermal growth factor receptor. procedure for its preparation. - Google Patents

Monoclonal anti-bodies which recognize the epidermal growth factor receptor. procedure for its preparation.

Info

Publication number
CU22387A1
CU22387A1 CU1992100A CU1992100A CU22387A1 CU 22387 A1 CU22387 A1 CU 22387A1 CU 1992100 A CU1992100 A CU 1992100A CU 1992100 A CU1992100 A CU 1992100A CU 22387 A1 CU22387 A1 CU 22387A1
Authority
CU
Cuba
Prior art keywords
growth factor
cells
monoclonal antibodies
antibodies
recognize
Prior art date
Application number
CU1992100A
Other languages
Spanish (es)
Inventor
Ordonez Alicia Fernandez
De Acosta Del Rio Christina Maria Mateo
Abraham Amparo Emilia Macias
Moreno Alvaro Enrique Velandia
Bravo Blanca Rosa Tormo
Rodriguez Rolando Perez
Original Assignee
Centro Inmunologia Molecular
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centro Inmunologia Molecular filed Critical Centro Inmunologia Molecular
Priority to CU1992100A priority Critical patent/CU22387A1/en
Priority to DK93202428T priority patent/DK0586002T3/en
Priority to EP93202428A priority patent/EP0586002B1/en
Priority to DE69327620T priority patent/DE69327620T2/en
Priority to ES93202428T priority patent/ES2144440T3/en
Priority to PT93202428T priority patent/PT586002E/en
Priority to AT93202428T priority patent/ATE188874T1/en
Publication of CU22387A1 publication Critical patent/CU22387A1/en
Priority to GR20000400972T priority patent/GR3033290T3/en

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

LA PRESENTE INVENCION SE RELACIONA CON LA RAMA DE LOS ANTICUERPOS MONOCLONALES Y SU UTILIZACION CON FINES DIAGNOSTICO, TERAPEUTICO E INVESTIGATIVO. ESTA INVENCION PROPORCIONA ANTICUERPOS MONOCLONALES QUE RECONOCEN EL RECEPTOR DEL FACTOR DE CRECIMIENTO EPIDERMICO (R-EGF) DE PLACENTA HUMANA, CON ALTA AFINIDAD Y CON CAPACIDAD DE INHIBIR LA UNION DEL EGF AL RECEPTOR. ESTOS ANTICUERPOS MONOCLONALES POSEEN TAMBIEN LA PROPIEDAD DE INHIBIR EL CRECIMIENTO TUMORAL EN CIERTAS LINEAS CELULARES, LA CAPACIDAD DE PRODUCIR ACTIVIDAD CELULAR CITOTOXICA DEPENDIENTE DE ANTICUERPO Y LA CAPACIDAD DE DESARROLLAR ACTIVIDAD CITOTOXICA MEDIADA POR COMPLEMENTO. LA ESENCIA DE LA INVENCION CONSISTE EN INMUNIZAR RATONES CON UNA FRACCION PARCIALMENTE PURIFICADA DEL RECEPTOR DEL FACTOR DE CRECIMIENTO EPIDERMICO DE PLACENTA HUMANA; EXTIRPAR SUS BAZOS Y PREPARAR UNA SUSPENSION DE LAS CELULAS; FUSIONAR LAS CELULAS ESPLENICAS CON CELULAS DE MIELOMA, EN PRESENCIA DE UN PROMOTOR DE FUSION; DILUIR Y CULTIVAR LAS CELU LAS; EVALUAR EL LIQUIDO SOBRENADANTE QUE CONTIENE EL HIBRIDOMA; SELECCIONAR Y CLONAR LOS HIBRIDOMAS PRODUCTORES DE ANTICUERPOS QUE REUNAN SIMULTANEAMENTE LAS CARACTERISTICAS REFERIDAS ANTERIORMENTE; RECUPERAR LOS ANTICUERPOS DEL LIQUIDO SOBRENADANTE DE LOS CLONES Y RECOGER LAS ASCITIS MALIGNAS O EL SUERO QUE CONTIENE LOS ANTICUERPOS DESEADOS.\!THE PRESENT INVENTION IS RELATED TO THE BRANCH OF MONOCLONAL ANTIBODIES AND THEIR USE FOR DIAGNOSTIC, THERAPEUTIC AND INVESTIGATIVE PURPOSES. THIS INVENTION PROVIDES MONOCLONAL ANTIBODIES THAT RECOGNIZE THE RECEPTOR OF THE EPIDERMIC GROWTH FACTOR (R-EGF) OF HUMAN PLACENTA, WITH HIGH AFFINITY AND ABILITY TO INHIBIT THE BINDING OF THE EGF TO THE RECEIVER. THESE MONOCLONAL ANTIBODIES ALSO HAVE THE PROPERTY OF INHIBITING TUMOR GROWTH IN CERTAIN CELL LINES, THE CAPACITY TO PRODUCE ANTIBODY CELLULAR ACTIVITY AND THE CAPACITY TO DEVELOP MEDOTALLY ACTIVITY. THE ESSENCE OF THE INVENTION IS TO IMMUNIZE MICE WITH A PARTIALLY PURIFIED FRACTION OF THE RECEPTOR OF THE EPIDERMIC GROWTH FACTOR OF PLACENTA HUMANA; REMOVE YOUR SPLITS AND PREPARE A SUSPENSION OF THE CELLS; MERGING SPLENIC CELLS WITH MYELOMA CELLS, IN THE PRESENCE OF A FUSION PROMOTER; DILUTE AND CULTIVATE CELLS; EVALUATE THE OVERLAPPING LIQUID CONTAINED IN THE HYBRIDOMA; SELECT AND CLONE ANTIBODY-PRODUCING HYBRIDOMES THAT SIMULTANEOUSLY COLLECT THE PREVIOUSLY REFERRED CHARACTERISTICS; TO RECOVER THE ANTIBODIES OF THE OVERLAPPING LIQUID OF THE CLONES AND TO COLLECT THE MALIGNANT ASCITIS OR THE SERUM CONTAINING THE DESIRED ANTIBODIES. \!

CU1992100A 1992-08-18 1992-08-18 Monoclonal anti-bodies which recognize the epidermal growth factor receptor. procedure for its preparation. CU22387A1 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
CU1992100A CU22387A1 (en) 1992-08-18 1992-08-18 Monoclonal anti-bodies which recognize the epidermal growth factor receptor. procedure for its preparation.
DK93202428T DK0586002T3 (en) 1992-08-18 1993-08-18 Monoclonal antibodies that recognize the epidermal growth factor receptor, its cells and methods, and preparations thereof
EP93202428A EP0586002B1 (en) 1992-08-18 1993-08-18 Monoclonal antibodies recognizing the epidermal growth factor receptor, cells and methods for their production and compositions containing them
DE69327620T DE69327620T2 (en) 1992-08-18 1993-08-18 Monoclonal antibodies to the epidermal growth factor receptor, cells and methods for their preparation and compositions containing them
ES93202428T ES2144440T3 (en) 1992-08-18 1993-08-18 MONOCLONAL ANTIBODIES THAT RECOGNIZE THE RECEPTOR OF THE EPIDERMIC GROWTH FACTOR, CELLS AND METHODS FOR ITS PRODUCTION AND COMPOUNDS THAT CONTAIN THEM.
PT93202428T PT586002E (en) 1992-08-18 1993-08-18 MONOCLONAL ANTIBODIES RECOGNIZING THE EPIDERMIC GROWTH FACTOR RECEIVER, CELLS AND METHODS FOR ITS PRODUCTION AND COMPOSITIONS THAT INCLUDE THEM
AT93202428T ATE188874T1 (en) 1992-08-18 1993-08-18 MONOCLONAL ANTIBODIES AGAINST THE EPIDERMAL GROWTH FACTOR RECEPTOR, CELLS AND METHODS FOR THE PRODUCTION THEREOF AND COMPOSITIONS CONTAINING THEM
GR20000400972T GR3033290T3 (en) 1992-08-18 2000-04-20 Arylazo chromoionophores.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU1992100A CU22387A1 (en) 1992-08-18 1992-08-18 Monoclonal anti-bodies which recognize the epidermal growth factor receptor. procedure for its preparation.

Publications (1)

Publication Number Publication Date
CU22387A1 true CU22387A1 (en) 1996-01-31

Family

ID=5459357

Family Applications (1)

Application Number Title Priority Date Filing Date
CU1992100A CU22387A1 (en) 1992-08-18 1992-08-18 Monoclonal anti-bodies which recognize the epidermal growth factor receptor. procedure for its preparation.

Country Status (1)

Country Link
CU (1) CU22387A1 (en)

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