CO6511252A2 - Sistema de extraccion de metales pesados - Google Patents
Sistema de extraccion de metales pesadosInfo
- Publication number
- CO6511252A2 CO6511252A2 CO12046735A CO12046735A CO6511252A2 CO 6511252 A2 CO6511252 A2 CO 6511252A2 CO 12046735 A CO12046735 A CO 12046735A CO 12046735 A CO12046735 A CO 12046735A CO 6511252 A2 CO6511252 A2 CO 6511252A2
- Authority
- CO
- Colombia
- Prior art keywords
- heavy metal
- bacteria
- extraction system
- provides
- gene expression
- Prior art date
Links
- 229910001385 heavy metal Inorganic materials 0.000 title abstract 5
- 238000000605 extraction Methods 0.000 title 1
- 241000894006 Bacteria Species 0.000 abstract 4
- 230000014509 gene expression Effects 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 abstract 1
- 229910052793 cadmium Inorganic materials 0.000 abstract 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 abstract 1
- 239000000356 contaminant Substances 0.000 abstract 1
- 101150066555 lacZ gene Proteins 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 abstract 1
- 229910052753 mercury Inorganic materials 0.000 abstract 1
- 210000002706 plastid Anatomy 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 230000009919 sequestration Effects 0.000 abstract 1
- 229910052725 zinc Inorganic materials 0.000 abstract 1
- 239000011701 zinc Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
- G01N33/521—Single-layer analytical elements
- G01N33/523—Single-layer analytical elements the element being adapted for a specific analyte
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Water Supply & Treatment (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Biodiversity & Conservation Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Inorganic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Processing Of Solid Wastes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
La invención proporciona un sistema de secuestro de metal pesado mediante bacterias. La bacteria expresa los genes ppk, mt, y/o P-galactosidasa (lacZ) y puede tolerar por lo menos 25 µM de mercurio, 1,000 µM de zinc, 250 µM de cadmio, y 3,000 µM de Pb. El sistema permite la determinación fácil de la presencia de contaminantes de metal pesado en un líquido y la recolección fácil de la bacteria que ha secuestrado grandes cantidades de metal pesado. Se proporciona adicionalmente un sistema de expresión de gen en bacterias que comprende elementos de expresión de gen de fago y plástido y suministra un nivel de expresión de proteína particularmente alto y resistencia al metal pesado.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23562409P | 2009-08-20 | 2009-08-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CO6511252A2 true CO6511252A2 (es) | 2012-08-31 |
Family
ID=43607596
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CO12046735A CO6511252A2 (es) | 2009-08-20 | 2012-03-20 | Sistema de extraccion de metales pesados |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US8785174B2 (es) |
| EP (1) | EP2467486B1 (es) |
| JP (1) | JP2013502223A (es) |
| KR (1) | KR101812629B1 (es) |
| CN (2) | CN102753691B (es) |
| AU (1) | AU2010284144B2 (es) |
| BR (1) | BR112012003726A2 (es) |
| CA (1) | CA2771528C (es) |
| CL (1) | CL2012000436A1 (es) |
| CO (1) | CO6511252A2 (es) |
| IL (1) | IL218196A (es) |
| NZ (1) | NZ598789A (es) |
| RU (1) | RU2571222C2 (es) |
| WO (1) | WO2011022590A2 (es) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011125619A1 (ja) * | 2010-04-08 | 2011-10-13 | 国立大学法人旭川医科大学 | 腸管保護剤 |
| CN103525849B (zh) * | 2013-10-24 | 2016-01-20 | 舒海燕 | 一种用于清除工业废水汞污染的重组质粒、构建方法、重组工程菌及应用 |
| CN104445845B (zh) * | 2014-10-31 | 2016-05-25 | 杭州师范大学 | 一种快速缓解重金属对厌氧氨氧化污泥活性抑制的方法 |
| CN106630493A (zh) * | 2015-07-26 | 2017-05-10 | 李娜 | 一种去除含镉污水污染物的方法 |
| US20170157013A1 (en) * | 2015-12-07 | 2017-06-08 | Colgate-Palmolive Company | Metal Amino Acid Complexes for Bacterial Aggregation |
| CN107916243A (zh) * | 2016-10-10 | 2018-04-17 | 中国科学院微生物研究所 | 用于处理重金属污染的微生物细胞和蛋白、相应的处理方法和试剂盒 |
| US10759683B2 (en) * | 2018-07-10 | 2020-09-01 | University Of Ottawa | Methods for removing mercury contaminant from aqueous solutions, and bioreactors therefor |
| CN109943571B (zh) * | 2019-04-11 | 2022-09-02 | 中国科学院遗传与发育生物学研究所农业资源研究中心 | 一种金属硫蛋白基因mt20、其编码得到的金属硫蛋白及其表达和应用 |
| CN110819551B (zh) * | 2019-07-02 | 2023-04-21 | 南阳师范学院 | 一株可阻控叶菜类蔬菜富集Cd和Pb的布甘氏肠杆菌CH6及其用途 |
| US11794893B2 (en) | 2020-09-08 | 2023-10-24 | Frederick William MacDougall | Transportation system for transporting organic payloads |
| EP4210860A4 (en) | 2020-09-08 | 2025-05-21 | MacDougall, Frederick William | CARBONIFICATION AND CARBON SEQUESTRATION USING DEEP-SEA HYDROTHERMAL BOREHOLE AIRHOLES |
| CN112961794B (zh) * | 2021-01-19 | 2023-01-03 | 西安中地环境科技有限公司 | 一种吸附汞的复合菌制剂及应用 |
| CN114181841A (zh) * | 2021-11-24 | 2022-03-15 | 浙江大学 | 治理汞污染的真菌、生物菌剂及应用和除汞方法以及鉴定治理汞污染能力真菌的方法 |
| CN114231459B (zh) * | 2021-12-27 | 2023-05-26 | 中南大学 | 一种大肠埃希氏菌、微生物菌剂、组合物及应用 |
| CN114152601B (zh) * | 2021-12-28 | 2023-09-15 | 军事科学院军事医学研究院环境医学与作业医学研究所 | 一种现场快速检测水中汞离子的方法、试剂盒及其应用 |
| PL443378A1 (pl) * | 2022-12-31 | 2024-07-01 | Instytut Biotechnologii I Medycyny Molekularnej | 7 aminokwasowe sekwencje oraz klony fagowe wiążące selektywnie jony niklu |
| CN116656586A (zh) * | 2023-03-17 | 2023-08-29 | 成都中医药大学 | 金属硫蛋白表面展示菌在川芎镉污染防治中的应用的方法 |
| CN118792323B (zh) * | 2024-08-21 | 2025-10-21 | 上海市农业科学院 | 一种在植物中串联表达的基因组合及其在提高植物耐镉性能中的应用 |
| CN119082152B (zh) * | 2024-08-21 | 2025-09-16 | 上海市农业科学院 | 提高植物富集镉和耐镉性能的基因组合、多基因表达盒与应用 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003011487A1 (en) * | 2001-07-27 | 2003-02-13 | Global Biosciences, Inc. | Remediation of metal contaminants with hydrocarbon-utilizing bacteria |
| US8440791B2 (en) * | 2001-09-06 | 2013-05-14 | Mgp Biotechnologies, Llc | Thimerosal removal device |
| RU2224604C1 (ru) * | 2002-06-27 | 2004-02-27 | Шулаева Раиса Васильевна | Способ ремедиации нефтезагрязненных грунтов |
| CA2631240A1 (en) * | 2004-12-03 | 2006-06-08 | Xoma Technology Ltd. | Methods and materials for expression of a recombinant protein |
| US20120124701A1 (en) * | 2008-02-01 | 2012-05-17 | Ceres, Inc. | Promoter, promoter control elements, and combinations, and use thereof |
-
2010
- 2010-08-19 US US12/859,590 patent/US8785174B2/en not_active Expired - Fee Related
- 2010-08-20 EP EP10810626.1A patent/EP2467486B1/en not_active Not-in-force
- 2010-08-20 WO PCT/US2010/046084 patent/WO2011022590A2/en not_active Ceased
- 2010-08-20 CA CA2771528A patent/CA2771528C/en active Active
- 2010-08-20 BR BR112012003726A patent/BR112012003726A2/pt not_active IP Right Cessation
- 2010-08-20 CN CN201080045997.2A patent/CN102753691B/zh not_active Expired - Fee Related
- 2010-08-20 RU RU2012110039/10A patent/RU2571222C2/ru active
- 2010-08-20 CN CN201410165439.XA patent/CN103992980A/zh active Pending
- 2010-08-20 AU AU2010284144A patent/AU2010284144B2/en not_active Ceased
- 2010-08-20 KR KR1020127007234A patent/KR101812629B1/ko not_active Expired - Fee Related
- 2010-08-20 NZ NZ598789A patent/NZ598789A/en not_active IP Right Cessation
- 2010-08-20 JP JP2012525707A patent/JP2013502223A/ja active Pending
-
2012
- 2012-02-19 IL IL218196A patent/IL218196A/en active IP Right Grant
- 2012-02-20 CL CL2012000436A patent/CL2012000436A1/es unknown
- 2012-03-20 CO CO12046735A patent/CO6511252A2/es not_active Application Discontinuation
-
2014
- 2014-05-30 US US14/292,668 patent/US9719096B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US9719096B2 (en) | 2017-08-01 |
| CL2012000436A1 (es) | 2013-03-08 |
| CN103992980A (zh) | 2014-08-20 |
| KR20120064078A (ko) | 2012-06-18 |
| JP2013502223A (ja) | 2013-01-24 |
| AU2010284144A1 (en) | 2012-04-12 |
| AU2010284144B2 (en) | 2015-07-23 |
| US8785174B2 (en) | 2014-07-22 |
| US20110220570A1 (en) | 2011-09-15 |
| EP2467486B1 (en) | 2016-04-06 |
| BR112012003726A2 (pt) | 2017-07-04 |
| CN102753691B (zh) | 2014-05-14 |
| KR101812629B1 (ko) | 2017-12-27 |
| WO2011022590A2 (en) | 2011-02-24 |
| NZ598789A (en) | 2013-12-20 |
| WO2011022590A3 (en) | 2011-07-28 |
| US20140273161A1 (en) | 2014-09-18 |
| EP2467486A4 (en) | 2013-06-12 |
| IL218196A0 (en) | 2012-04-30 |
| CN102753691A (zh) | 2012-10-24 |
| CA2771528C (en) | 2018-10-30 |
| IL218196A (en) | 2016-05-31 |
| RU2012110039A (ru) | 2013-09-27 |
| CA2771528A1 (en) | 2011-02-24 |
| RU2571222C2 (ru) | 2015-12-20 |
| EP2467486A2 (en) | 2012-06-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FC | Application refused |