CN2488066Y - Chromatographic column for separating and purifying natural product - Google Patents
Chromatographic column for separating and purifying natural product Download PDFInfo
- Publication number
- CN2488066Y CN2488066Y CN 01244876 CN01244876U CN2488066Y CN 2488066 Y CN2488066 Y CN 2488066Y CN 01244876 CN01244876 CN 01244876 CN 01244876 U CN01244876 U CN 01244876U CN 2488066 Y CN2488066 Y CN 2488066Y
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- China
- Prior art keywords
- discharging
- charging
- separation
- natural products
- filtrator
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 229930014626 natural product Natural products 0.000 title claims abstract description 17
- 238000007599 discharging Methods 0.000 claims abstract description 56
- 239000000463 material Substances 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 37
- 238000000926 separation method Methods 0.000 claims description 24
- 239000012528 membrane Substances 0.000 claims description 15
- 239000000945 filler Substances 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 3
- 238000005265 energy consumption Methods 0.000 abstract 1
- 238000009776 industrial production Methods 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 abstract 1
- 238000000746 purification Methods 0.000 description 13
- 238000010586 diagram Methods 0.000 description 5
- 239000002904 solvent Substances 0.000 description 5
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 3
- JEVVKJMRZMXFBT-XWDZUXABSA-N Lycophyll Natural products OC/C(=C/CC/C(=C\C=C\C(=C/C=C/C(=C\C=C\C=C(/C=C/C=C(\C=C\C=C(/CC/C=C(/CO)\C)\C)/C)\C)/C)\C)/C)/C JEVVKJMRZMXFBT-XWDZUXABSA-N 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- OAIJSZIZWZSQBC-GYZMGTAESA-N lycopene Chemical compound CC(C)=CCC\C(C)=C\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C=C(/C)CCC=C(C)C OAIJSZIZWZSQBC-GYZMGTAESA-N 0.000 description 3
- 229960004999 lycopene Drugs 0.000 description 3
- 235000012661 lycopene Nutrition 0.000 description 3
- 239000001751 lycopene Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- ZCIHMQAPACOQHT-ZGMPDRQDSA-N trans-isorenieratene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/c1c(C)ccc(C)c1C)C=CC=C(/C)C=Cc2c(C)ccc(C)c2C ZCIHMQAPACOQHT-ZGMPDRQDSA-N 0.000 description 3
- 238000007598 dipping method Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
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- 239000004615 ingredient Substances 0.000 description 2
- 229920002477 rna polymer Polymers 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
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- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- YKPUWZUDDOIDPM-SOFGYWHQSA-N capsaicin Chemical compound COC1=CC(CNC(=O)CCCC\C=C\C(C)C)=CC=C1O YKPUWZUDDOIDPM-SOFGYWHQSA-N 0.000 description 1
- 229960002504 capsaicin Drugs 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
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- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
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- 239000000126 substance Substances 0.000 description 1
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- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
A chromatographic column for separating and purifying equipment of natural product comprises a column body, wherein both ends of the column body are respectively provided with a feeding cover and a discharging cover of a feeding port and a discharging port; the inside of the column body is a filler column made from adsorption material; the center of the filler column is provided with a central through hole; a feeding filter and a discharging filter are respectively arranged between the feeding cover and the filler column as well as the discharging cover and the filler column; in addition, the central through hole is respectively communicated with the feeding port and the discharging port through the feeding filter and the discharging filter. The utility model possesses better applying effect and can meet the requirements of industrial production; meanwhile, with high production efficiency and low energy consumption, the utility model can effectively reduce cost, thereby having better social and economic benefits.
Description
Technical field
The utility model relates to a kind of biologic and extracts the separation equipment field, particularly relates to being applicable to the device that pluck organ, natural plants, sea-plant, Chinese crude drug effective ingredient are carried out separation and purification.
Background technology
Biotechnology is for solving the new way that human food that is faced and significant problems such as nutrition, resource and the energy, environment and health are opened up, by the separation and purification of biotechnology to the effective ingredient of product, obtain various effective monomer gene outcomes, be the effective product in the genetic engineering such as RNA (ribonucleic acid), interferon, protide and monoclonal anti voxel etc.; Glycosides displayed in the Chinese crude drug, alkaloid, microbiotic etc.; Lycopene in natural colouring matter in the biochemical product, chlorophyll etc. and the food, capsaicine, natural VE, phosphatide and carbohydrate etc.
Yet one of gordian technique of biotechnology is the separation of product and purifying, legacy equipment is still continued to use in present separation, be that single jar of dipping extraction of single-stage and dispersion contact digester carry out separation and purification to product, not only production efficiency is low, energy resource consumption is big, solvent consumption is serious, and is difficult to satisfy the separation and purification of high performance dielectric.
Summary of the invention
The purpose of this utility model is a kind of production efficiency height of design, simple in structure, with low cost and can satisfy the Separation of Natural Products purifying chromatographic column of high performance dielectric separation and purification.
The technical scheme that realizes above-mentioned purpose is as follows: Separation of Natural Products equipment chromatographic column, has a cylinder, its two ends are feed cap and the discharging lid that is respectively arranged with charging aperture and discharging opening, filled column for making in the cylinder by sorbing material, filled column central authorities have central through hole, be respectively arranged with charging filtrator and discharging filtrator between feed cap, discharging lid and filled column, central through hole communicates with discharging opening with charging aperture respectively by charging filtrator, discharging filtrator.
Further technical measures according to aforementioned schemes, described charging filtrator has back up pad, the charging fair water fin that is fixed on the feed cap and is in filter membrane between the above two, the discharging filtrator has back up pad, the discharging fair water fin that the discharging of being fixed on covers and is between the two filter membrane, have uniform support plate hole on the back up pad, filter membrane is made up of lattice-shaped filter membrane hole.
By aforementioned, on the described discharging fair water fin, have some roads circumference diversion trench concentric with its center of circle, have on each diversion trench and run through discharging fair water fin water conservancy diversion slotted eye.
By aforementioned, have the charging water conservancy diversion plate hole corresponding on the described charging fair water fin with aforementioned support plate hole, with some roads circumference of charging fair water fin concentric on, have uniform colored core groove.
Further again, described feed cap and discharging lid are for the internal thread lid, with the cylinder thread connection.
By aforesaid another technical measures, described with some roads circumference of charging fair water fin concentric on, have uniform colored core groove.
According to another technical measures of aforementioned techniques scheme, described cylinder is " U " shape post, and its filled column, central through hole also are " U " shape.
Further, described feed cap, discharging lid and cylinder pass through Bolt Connection.
According to aforesaid further technical measures, described cylinder has multi-segmental, and is fixedly connected by combination sub between each sections.
Of the present utility model have an effect preferably, can satisfy industrialization production, can be as required, only need the conversion solvent, can separation and purification go out the multiple components in the natural products, so production efficiency height, energy resource consumption are low, can effectively reduce cost, have good social and economic benefit.
Description of drawings
Fig. 1 is the structural representation of embodiment one;
Fig. 2 is a charging fair water fin synoptic diagram among Fig. 1;
Fig. 3 is a filter membrane synoptic diagram among Fig. 1;
Fig. 4 is a back up pad synoptic diagram among Fig. 1;
Fig. 5 is a discharging fair water fin synoptic diagram among Fig. 1;
Fig. 6 is the structural representation of embodiment two;
Fig. 7 is a charging fair water fin synoptic diagram among Fig. 6;
Fig. 8 is the structural representation of embodiment three;
The 1-cylinder; The 2-feed cap; 3-discharging lid; The 4-filled column; 5-charging filtrator; 6-discharging filtrator; The 11-combination sub; The 21-charging aperture; The 31-discharging opening; The 41-central through hole; 51-charging fair water fin; 61-discharging fair water fin; The 7-filter membrane; The 8-back up pad; 511-charging water conservancy diversion plate hole; 512-flower core groove; 71-filter membrane hole; 81-supports plate hole; 611-circumference diversion trench; 612-water conservancy diversion slotted eye; The 9-bolt.Need to prove that among embodiment one and the embodiment two, three, it is obviously different that feed cap and the structure of discharging lid, charging fair water fin have, feed cap, discharging lid are also different with the connecting mode of cylinder, all the other component structural all similar.
Embodiment
Below in conjunction with description of drawings embodiment of the present utility model:
Embodiment one: be a kind of structure that comparatively generally adopts in the utility model.Cylinder 1 three sections, fixedly connected between each sections by combination sub 11, cylinder 1 two ends are covered 3 thread connection with the feed cap 2 that is respectively arranged with charging aperture 21 and discharging opening 31 and has an internal thread with discharging, filled column 4 for making in the cylinder 1 by sorbing material, filled column 4 central authorities have central through hole 41, feed cap 2, discharging lid 3 and 4 of filled columns are respectively arranged with charging filtrator 5 and discharging filtrator 6, and central through hole 41 communicates with discharging opening 31 with charging aperture 21 respectively by charging filtrator 5, discharging filtrator 6.Charging filtrator 5 has back up pad 8, the charging fair water fin 51 that is fixed on the feed cap 2 and is in filter membrane 7 between the above two, discharging filtrator 6 has back up pad 8, the discharging fair water fin 61 and the filter membrane 7 that is between the two on the discharging of being fixed on lid 3, have uniform support plate hole 81 on the back up pad 8, filter membrane 7 is made up of lattice-shaped filter membrane hole 71.On the discharging fair water fin 61, have some roads circumference diversion trench 611 concentric, have on each diversion trench 611 and run through discharging fair water fin water conservancy diversion slotted eye 612 with its center of circle.Have the charging water conservancy diversion plate hole 511 corresponding on the charging fair water fin 51 with supporting plate hole 81, with some roads circumference of charging fair water fin 51 concentrics on, have uniform colored core groove 512.
Embodiment two: the chromatographic column that is a kind of ultrashort type in the utility model.Be with the difference of embodiment one: cylinder has only a sections, and feed cap connects with cylinder by bolt 9 with the discharging lid, and in addition, charging fair water fin 51 and embodiment one difference are not have charging water conservancy diversion plate hole 511 thereon, and colored core groove 512 is only arranged.The miscellaneous part structure is similar to embodiment.
Embodiment three: be another chromatographic column of the present utility model.Cylinder 1 is " U " shape post, and its filled column 4, central through hole 41 also are " U " shape.Its charging aperture 21 and discharging opening 31 on same surface level, respectively have a joint straight section and the curved section of a joint in use about its cylinder, each is intersegmental also by combination sub 11 connections.Remaining part is similar to embodiment two.
For ease of understanding the utility model, sketch principle of work (principle of work of above-mentioned three embodiment is similar) below with reference to each parts:
Material liquid (natural products is through squeezing the concentrate after broken) is entered the charging filtrator through charging aperture, effect because of the charging fair water fin, material liquid enters in the filler of filled column, and can not directly enter central through hole, the filler of filled column is to decide according to the material of chromatographic column desire separation and purification, it should adsorb the material that needs purification, and allows the material that need not purify flow into central through hole with liquid, comes out to reclaim from discharging opening through the discharging filtrator again.And then from charging aperture feeding solvent, this solvent can dissolve the material (desire purification thing) that is adsorbed by filled column, this process claims " wash-out " on industry, solution behind the dissolving purification thing flows out from discharging opening through the discharging filtrator, like this, as long as this solution is carried out can obtaining after solvent evaporation, the solute drying purifying substances of based on very high purity.The utility model can be widely used in the purification of natural products on the biotechnology, as carry lycopene in the tomato of to purify, the finished product lycopene is very expensive, price is closed 500,000 yuan of Renminbi per kilograms in the world, and purification originally is by single jar of dipping extraction of single-stage and disperse the contact digester that product is carried out separation and purification, and output is very low, and purity is difficult to reach requirement, use the separation equipment of chromatographic column of the present utility model to purify, output is multiplied, and purity improves greatly.What deserves to be mentioned is in addition, after the material that is not adsorbed in aforementioned reclaims, owing to wherein also have other valuable materials, if required, can select appropriate filler and eluting solvent, just other useful material can be purified again, so just improve the value of raw material thing greatly.Along with continually developing of the continuous development of life science, biotechnology, the utility model will obtain utilization more and more widely.
Claims (9)
1, Separation of Natural Products equipment chromatographic column, has a cylinder, it is characterized in that: its two ends are feed cap and the discharging lid that is respectively arranged with charging aperture and discharging opening, filled column for making in the cylinder by sorbing material, filled column central authorities have central through hole, be respectively arranged with charging filtrator and discharging filtrator between feed cap, discharging lid and filled column, central through hole communicates with discharging opening with charging aperture respectively by charging filtrator, discharging filtrator.
2, Separation of Natural Products equipment chromatographic column according to claim 1, it is characterized in that: described charging filtrator has back up pad, the charging fair water fin that is fixed on the feed cap and is in filter membrane between the above two, the discharging filtrator has back up pad, the discharging fair water fin that the discharging of being fixed on covers and is between the two filter membrane, have uniform support plate hole on the back up pad, filter membrane is made up of lattice-shaped filter membrane hole.
3, Separation of Natural Products equipment chromatographic column according to claim 2 is characterized in that: on the described discharging fair water fin, have some roads circumference diversion trench concentric with its center of circle, have on each diversion trench and run through discharging fair water fin water conservancy diversion slotted eye.
4, according to claim 2 or 3 described Separation of Natural Products equipment chromatographic columns, it is characterized in that: have the charging water conservancy diversion plate hole corresponding on the described charging fair water fin with aforementioned support plate hole, with some roads circumference of charging fair water fin concentric on, have uniform colored core groove.
5, Separation of Natural Products equipment chromatographic column according to claim 4 is characterized in that: described feed cap and discharging lid are for the internal thread lid, with the cylinder thread connection.
6, according to claim 2 or 3 described Separation of Natural Products equipment chromatographic columns, it is characterized in that: described with some roads circumference of charging fair water fin concentric on, have uniform colored core groove.
7, Separation of Natural Products equipment chromatographic column according to claim 6, it is characterized in that: described cylinder is " U " shape post, its filled column, central through hole also are " U " shape.
8, Separation of Natural Products equipment chromatographic column according to claim 7, it is characterized in that: described feed cap, discharging lid and cylinder pass through Bolt Connection.
9, according to claim 1 or 2 or 3 described Separation of Natural Products equipment chromatographic columns, it is characterized in that: described cylinder has multi-segmental, and is fixedly connected by combination sub between each sections.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01244876 CN2488066Y (en) | 2001-07-16 | 2001-07-16 | Chromatographic column for separating and purifying natural product |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01244876 CN2488066Y (en) | 2001-07-16 | 2001-07-16 | Chromatographic column for separating and purifying natural product |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2488066Y true CN2488066Y (en) | 2002-04-24 |
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ID=33656221
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01244876 Expired - Fee Related CN2488066Y (en) | 2001-07-16 | 2001-07-16 | Chromatographic column for separating and purifying natural product |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2488066Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100350245C (en) * | 2002-08-02 | 2007-11-21 | 比奥尼西斯股份有限公司 | Device for separating sample components by liquid chromatography under pressure |
| CN102422156A (en) * | 2009-05-15 | 2012-04-18 | 通用电气健康护理生物科学股份公司 | Filter holder in a chromatography column |
| CN106474769A (en) * | 2016-12-30 | 2017-03-08 | 北京创新通恒科技有限公司 | A kind of discharge member and chromatographic column |
-
2001
- 2001-07-16 CN CN 01244876 patent/CN2488066Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100350245C (en) * | 2002-08-02 | 2007-11-21 | 比奥尼西斯股份有限公司 | Device for separating sample components by liquid chromatography under pressure |
| CN102422156A (en) * | 2009-05-15 | 2012-04-18 | 通用电气健康护理生物科学股份公司 | Filter holder in a chromatography column |
| CN102422156B (en) * | 2009-05-15 | 2014-04-16 | 通用电气健康护理生物科学股份公司 | Filter holder in a chromatography column |
| CN106474769A (en) * | 2016-12-30 | 2017-03-08 | 北京创新通恒科技有限公司 | A kind of discharge member and chromatographic column |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |