CN2336359Y - Self-supporting ceramic plate polyacrylamide gel electrophoresis apparatus - Google Patents
Self-supporting ceramic plate polyacrylamide gel electrophoresis apparatus Download PDFInfo
- Publication number
- CN2336359Y CN2336359Y CN 98224007 CN98224007U CN2336359Y CN 2336359 Y CN2336359 Y CN 2336359Y CN 98224007 CN98224007 CN 98224007 CN 98224007 U CN98224007 U CN 98224007U CN 2336359 Y CN2336359 Y CN 2336359Y
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- Prior art keywords
- groove
- glue loading
- porcelain plate
- self
- sample
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- Expired - Fee Related
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- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 title claims abstract description 16
- 239000000919 ceramic Substances 0.000 title abstract 6
- 239000011521 glass Substances 0.000 claims abstract description 30
- 229920001971 elastomer Polymers 0.000 claims abstract description 8
- 229910052573 porcelain Inorganic materials 0.000 claims description 61
- 239000003292 glue Substances 0.000 claims description 41
- 244000126211 Hericium coralloides Species 0.000 claims description 9
- 229910052571 earthenware Inorganic materials 0.000 claims description 9
- 210000001520 comb Anatomy 0.000 abstract 2
- 238000007789 sealing Methods 0.000 abstract 1
- 238000001962 electrophoresis Methods 0.000 description 8
- 238000010586 diagram Methods 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 239000004519 grease Substances 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 230000000750 progressive effect Effects 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 240000008790 Musa x paradisiaca Species 0.000 description 1
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000006664 bond formation reaction Methods 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000005755 formation reaction Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000010068 moulding (rubber) Methods 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000012882 sequential analysis Methods 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 239000004945 silicone rubber Substances 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000007693 zone electrophoresis Methods 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model provides a self-supporting ceramic plate polyacrylamide gel electrophoresis apparatus, comprising a lower groove and a self-supporting upper groove in the lower groove, wherein, the upper groove comprises front and back grooving gel-carrying ceramic plates; the vertical edges of the front and back grooving gel-carrying ceramic plates are provided with ceramic interlaced strips, then are covered with a front and a back glass plates, and are clamped to be fixed by a plurality of long tail clamps; the bottom of the utility model is sleeved and sealed by a bottom-sealing rubber sleeve, and is additionally provided with sample-groove combs. The utility model is characterized in that the front and back grooving gel-carrying ceramic plates are respectively provided with a flat-bottomed groove opening of which groove width accords with the front and back sample-groove combs; height difference exists in groove positions of the flat-bottomed groove openings; the groove openings or the glass plates are provided with permanent serial-number identification marks corresponding to the position of the sample-groove comb teeth.
Description
The utility model relates to a kind of improvement that is used for the electrophoretic apparatus of molecular biology, biological chemistry, specifically, is a kind of permanent marker parts porcelain plate gel electrophoresis apparatus that contains.
Polyacrylamide gel electrophoresis (PAGE) is the zone electrophoresis technology in a kind of Biochemistry and Molecular Biology field that grows up nineteen fifties.At protein, the purity of polypeptide (homogeneity), molecular weight, isoelectric point, multiple mensuration such as molecular radius, the two-phase electrophoresis is widely used in the sequential analysis of trace electrophoretic blotting and nucleic acid.Also can be used to prepare the PAGE of above-mentioned biomolecule, two kinds of tubulose and flat boards are arranged; The former comprises conventional and kapillary PAGE; The latter has level and vertical slab electrophoresis not.Not following ten kinds in vertical panel PAGE device both domestic and external with regard to material and structure, can be divided into following several with regard to its main member:
1, full bonding glass formula: removing the sample cell comb, outside electric component and the geometrical clamp, all is glass plate, and bar constitutes, and this is the most original.
2, plastics or organic glass are mixed two glass plates; Bar (spacer) between each embedding one rubber of its right and left or organic glass.
3, how not (Hoefer) purchased by peace Pharmacia Bioisystech Co., Ltd (Amersham pharmaciaBiotech).Their original small-sized (mini) plate electrophoresis device of making and Sigma (Sigma) company and Bole company (BioRad) similar; Product all is furnished with and similarly opens vat class porcelain plate, bar between rubber.Lean between class porcelain plate and the cathode can among silicone rubber tube (often wanting grease) Jie in case leakage; Whole device is arranged in pairs or groups in use: groove+porcelain plate on the plastics+bar+glass plate, fix with clip.
4, the product MV-1 of Dalian Jie Mai company transplants above-mentionedly 1,3, promptly replaces the class porcelain plate that improvement is not arranged as yet with fluting glass plate, and on the contrary, seeming regresses to some extent.
5, another kind of at last the device of the V16 sandwich of Shanghai lean factory (organic glass+rubber strip+two glass plate) electrophoresis tank with cooling characteristics.
6, Bole BioRad ProteanTM II (the transparent II type of protein nucleic acid); And mini Protean II (protein nucleic acid platelet II type) is relatively large and small-sized dull and stereotyped PAGE device.Said apparatus basic characteristics or advantage are: the assembling back is as one; Secondly, majority has cooling-part, and then result of use is fair.But they exist a bit following or some weak point respectively in various degree:
(1) assembling is loaded down with trivial details, aftertreatment waste time and energy (as above-mentioned 5,6).Even the glass plate of when assembling, easily crushing.
(2) the structure design is unreasonable, if select the veneer electrophoresis, needs to do anti-drain process (as 2).
(3) behind the glue, extract sample groove comb (comb) out before, majority need be done temporary marker (as 1-6) at the broach place.
(4) some will grease (as 3,4) or Ago-Gel (as 1,2,4 and 5) leakproof on parts.Increase the washing odd job.
(5) some reagent (above-mentioned 1-6) that take more.
(6) on flat board, be difficult to do small size gel electrophoresis (1-6).
(7) some device need be joined back cover leakproof glue in addition (as (1-5).
(8) tend to gappedly between hydrophobicity between bar and gel, easily cause both sides sample electrophoresis behavior deformity, to preparation with analyze neither profit (2,3,4,6).
(9) device that has (1,2) heat radiation is bad, impact effect and efficient.
(10) some need add encapsulating equipment (4); Without exception on the high side of above-mentioned 1-6 all will increase the experiment expenditure.
The purpose of this utility model is the shortcoming that overcomes prior art, and a kind of self-supporting earthenware slab polyacrylamide gel electrophoresis device is provided.
The utility model is to realize like this, it comprises that one has under the porcelain of electrical socket groove on the groove and a porcelain that has electrical socket that can stand in certainly in this time groove, before groove contains on this porcelain, back fluting glue loading porcelain plate, before this, the vertical edges of back fluting glue loading porcelain plate establish between porcelain bar again coated with this before, back fluting glue loading porcelain plate match before, back glass plate, and respectively by some long-tail folder clamping corresponding this year of offset plates, between bar and glass plate, their bottom is sealed by back cover rubber condom, other has sample groove comb and preceding fluting glue loading porcelain plate, the preceding sample groove comb that the front glass flat board matches and with back fluting glue loading porcelain plate, the rear sample groove comb that back glass plate matches, before it is characterized in that this, back fluting glue loading porcelain plate offer respectively with before, the corresponding to flat little notch of the groove width of rear sample groove comb, there is difference in height in the groove position between them.
Further, be provided with the corresponding to sulculus of broach number in the utility model in the notch of this forward and backward fluting glue loading porcelain plate with sample groove comb, and become indenting with the broach of sample groove comb, and there is difference in height in the groove position of the sulculus of the groove position of the sulculus of the glue loading porcelain plate of should precedingly slotting and back fluting glue loading porcelain plate; Or the sulculus on this forward and backward fluting glue loading porcelain plate is provided with the corresponding permanent sequence number distinguishing mark in sample groove comb tooth position or this forward and backward glass plate sulculus corresponding to this forward and backward fluting glue loading porcelain plate and is provided with and the corresponding permanent sequence number distinguishing mark in sample groove comb tooth position, or the notch appropriate location of this forward and backward fluting glue loading porcelain plate is provided with and the corresponding permanent sequence number distinguishing mark in sample groove comb tooth position; This forward and backward glass plate is provided with and the corresponding permanent sequence number distinguishing mark in sample groove comb tooth position corresponding to the notch place of this forward and backward fluting glue loading porcelain plate.
Progressive effect of the present utility model is:
1, assembling simple and convenient, general needed to finish in 2～5 minutes.
2, reasonable in design, forward and backward fluting glue loading porcelain plate both can be used as groove from staking, the groove position because of the fluting between them there are differences again, so help the user, can only need select the low glue loading porcelain plate in groove position for use when only needing single face (piece) plate electrophoresis.
3, be provided with and the corresponding permanent sequence number distinguishing mark in sample groove comb tooth position by forward and backward fluting glue loading porcelain plate or forward and backward glass plate, so, in case the encapsulating polymerization is finished, behind the extraction sample groove comb, make the user feel the convenience that application of sample is easy, bright, accurate, fast.
The utility model accompanying drawing is simply described as follows:
Fig. 1 is a three-dimensional external form synoptic diagram of the present utility model.
Fig. 2 is the exploded perspective synoptic diagram of a kind of embodiment of the present utility model.
Fig. 3 is the exploded perspective synoptic diagram of another kind of embodiment of the present utility model.
Fig. 4 is that schematic perspective view is shown in the decomposition of another embodiment in addition of the present utility model.
Below, we provide the utility model embodiment preferably according to Fig. 1～Fig. 4, so that architectural feature of the present utility model and progressive effect can be described better, this is for the utility model being described rather than limiting hold in range of the present utility model.
As depicted in figs. 1 and 2, the utility model comprises groove 1 down, with last groove 2, they have electrical socket 11 respectively, 21, should go up groove 2 from standing in this time groove 1, on the present embodiment, following groove 2,1 is made by pottery, please cooperate consult shown in Figure 2, last groove 2 comprises by preceding fluting glue loading porcelain plate 22, the ㄩ type porcelain piece 24 bonding formations of back fluting glue loading porcelain plate 23 and formation bottom land and left and right sides sidewall, in this example, the end of this ㄩ type porcelain piece 24, is rectangular, its long limit is approximately than before this, back fluting glue loading porcelain plate 22, little 8～the 10mm of 23 width, wide 2/3 of the length that is about, thick about 3mm, the top of two sides is isosceles trapezoid, a circular hole is respectively arranged on the center line of two trapezoidal faces, the center of this circular hole and its coboundary are apart from about 5mm, this circular hole is installed electrical socket 21 (black banana bin) and platinum filament, before being somebody's turn to do, back fluting glue loading porcelain plate 22,23 bottom form groove 2 from staking, before promptly being somebody's turn to do, back fluting glue loading porcelain plate 22,23 have support, the effect of heat radiation, the about 1mm of thickness, this porcelain plate 22, it is 10～15mm that 23 tops have a height (degree of depth), the U-shaped vat 221 of wide about 66mm, 231, and the groove position of vat 231 is than vat 221 height, general difference is greater than 1mm, be convenient to like this be used to use as single sided board, at this two porcelain plate 22, two vertical edges (left sides of 23, the right side) establishes bar 25 between porcelain, between this porcelain the length of bar 25 with should before, back fluting glue loading porcelain plate 22,23 long limit equates, width 10mm, thickness 1mm, usually thickness will determine the thickness of polyacrylamide gel (PAG), it also is into the part of rubber moulding tool, generally have bar 25 between 4, wherein two predetermined fixed (left side, 22 liang of edges of glue loading porcelain plate of before this, slotting, the right side) on, and the alignment of their bottom.Topped respectively going forward on the bar 25 between also having then, back glass plate 26,27, at last with before some the long-tail folder clampings, back fluting glue loading porcelain plate 22,23, between porcelain bar 25 and before, back glass plate 26,27, and by back cover rubber sleeve 28 their bottoms of cover envelope, before being somebody's turn to do, back glass plate 26,27 thickness 1mm, greatly, little and this porcelain plate 22,23 match, its function is to be used for irritating casting PAG and observation, monitoring electrophoresis overall process, by this porcelain plate 22,23, between bar 25, glass plate 26,27 and the empty part in centre that surrounds of back cover rubber sleeve 28, can hold the usefulness of pouring into gel.Also has sample groove comb 29.Again as seen, be provided with and the corresponding permanent sequence number distinguishing mark in sample groove comb 29 tooth positions in the appropriate location of the U-shaped vat 221,231 of forward and backward fluting glue loading porcelain plate 22,23: for example: ABCDEFGHIJK from Fig. 2; 0123456789.
See also Fig. 3 again, as shown in the figure, in the U-shaped vat 221 of this forward and backward fluting glue loading porcelain plate 22,23,23l, be provided with and the corresponding permanent mark piece in sample groove comb 29 tooth positions, for example offer well-regulated little groove 222.
Other sees also Fig. 4, as shown in the figure, is provided with on this forward and backward glass plate 26,27 and the corresponding permanent sequence number recognition device in the tooth position of sample groove comb 29, for example is provided with abcdefghijk; 01234567890.
Claims (7)
1, a kind of self-supporting earthenware slab polyacrylamide gel electrophoresis device, comprise that one has under the porcelain of electrical socket groove on the groove and a porcelain that has electrical socket that can stand in certainly in this time groove, before groove contains on this porcelain, back fluting glue loading porcelain plate, before this, the vertical edges of back fluting glue loading porcelain plate establish between porcelain bar again coated with this before, back fluting glue loading porcelain plate match before, back glass plate, and respectively by some long-tail folder clamping corresponding this year of offset plates, between bar and glass plate, their bottom is sealed by back cover rubber condom, other has sample groove comb and preceding fluting glue loading porcelain plate, the preceding sample groove comb that the front glass flat board matches and with back fluting glue loading porcelain plate, the rear sample groove comb that back glass plate matches, before it is characterized in that this, back fluting glue loading porcelain plate offer respectively with before, the corresponding to flat notch of the groove width of rear sample groove comb, there is difference in height in the groove position between them.
2, self-supporting earthenware slab polyacrylamide gel electrophoresis device according to claim 1 is characterized in that being provided with in the notch of this forward and backward fluting glue loading porcelain plate the corresponding to sulculus of broach number with sample groove comb, and becomes interlocking with the broach of sample comb groove.
3, self-supporting earthenware slab polyacrylamide gel electrophoresis device according to claim 2 is characterized in that this is preceding, there is difference in height in the groove position of the sulculus of fluting glue loading porcelain plate with the groove position of the sulculus of back fluting glue loading porcelain plate.
4, self-supporting earthenware slab polyacrylamide gel electrophoresis device according to claim 2 is characterized in that the sulculus on this forward and backward fluting glue loading porcelain plate is provided with and the corresponding permanent sequence number distinguishing mark in sample groove comb tooth position.
5, self-supporting earthenware slab polyacrylamide gel electrophoresis device according to claim 2 is characterized in that this forward and backward glass plate is provided with and the corresponding permanent sequence number distinguishing mark in sample groove comb tooth position corresponding to the sulculus of this forward and backward fluting glue loading porcelain plate.
6, self-supporting earthenware slab polyacrylamide gel electrophoresis device according to claim 1 is characterized in that the notch appropriate location of this forward and backward fluting glue loading porcelain plate is provided with and the corresponding permanent sequence number distinguishing mark in sample groove comb tooth position.
7, self-supporting earthenware slab polyacrylamide gel electrophoresis device according to claim 1 is characterized in that this forward and backward glass plate is provided with and the corresponding permanent sequence number distinguishing mark in sample groove comb tooth position corresponding to the notch place of this forward and backward fluting glue loading porcelain plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 98224007 CN2336359Y (en) | 1998-05-14 | 1998-05-14 | Self-supporting ceramic plate polyacrylamide gel electrophoresis apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 98224007 CN2336359Y (en) | 1998-05-14 | 1998-05-14 | Self-supporting ceramic plate polyacrylamide gel electrophoresis apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2336359Y true CN2336359Y (en) | 1999-09-01 |
Family
ID=33974969
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 98224007 Expired - Fee Related CN2336359Y (en) | 1998-05-14 | 1998-05-14 | Self-supporting ceramic plate polyacrylamide gel electrophoresis apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2336359Y (en) |
-
1998
- 1998-05-14 CN CN 98224007 patent/CN2336359Y/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |