CN2350006Y - Laser microbeam apparatus for microsurgery - Google Patents
Laser microbeam apparatus for microsurgery Download PDFInfo
- Publication number
- CN2350006Y CN2350006Y CN98251839U CN98251839U CN2350006Y CN 2350006 Y CN2350006 Y CN 2350006Y CN 98251839 U CN98251839 U CN 98251839U CN 98251839 U CN98251839 U CN 98251839U CN 2350006 Y CN2350006 Y CN 2350006Y
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- Prior art keywords
- laser
- instrument
- diaphragm
- light path
- connects
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- Expired - Fee Related
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- 238000002406 microsurgery Methods 0.000 title claims abstract description 9
- 239000013078 crystal Substances 0.000 claims abstract description 8
- 239000007787 solid Substances 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 229910052724 xenon Inorganic materials 0.000 claims description 2
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 claims description 2
- 230000008033 biological extinction Effects 0.000 claims 1
- 230000006378 damage Effects 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 2
- 230000001105 regulatory effect Effects 0.000 abstract 2
- 238000001816 cooling Methods 0.000 abstract 1
- 230000008030 elimination Effects 0.000 abstract 1
- 238000003379 elimination reaction Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 10
- 230000003287 optical effect Effects 0.000 description 8
- 230000004075 alteration Effects 0.000 description 4
- 239000012472 biological sample Substances 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000031700 light absorption Effects 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007159 enucleation Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 210000004895 subcellular structure Anatomy 0.000 description 1
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Abstract
The utility model relates to a laser microbeam apparatus for microsurgery. The utility model comprises a solid laser (Nd: YAG), wherein the solid-state laser is connected with a laser power supply and is connected with a laser control panel, and a Q regulating crystal and a Q regulating circuit of the solid laser are connected; the head part of the solid-state laser exactly faces to a fully permeable film of 45 DEG in full reflection of 532 nm to 1064 nm, a lens with a long focus and a full reflecting film by a light path. A microscope is arranged on the light path of the full reflecting film, a light absorbing barrel is arranged on a reflecting light path of the fully permeable film, and the head of the solid-state laser is provided with a cooling device. The utility model has the advantages of small size, monochromaticity, color difference elimination and adjustable output energy and has high spatial resolution to not damage cells.
Description
This utility model relates to a kind of medical apparatus and instruments, or relates to the analysis apparatus of a kind of medical science and field of biology.
The way that present expeimental physics and experimental biology are relied on is to separate earlier or the change subsystem, studies the whole behavior that has become system then.If interest is at cell function, just need to get rid of the intracellular entity of size in micron, microsurgery cooperates suitable optical element with common electric filament lamp, the device of its composition not only heavily but also big, owing to the optics selection, the loss of intensity also is surprising.So far, microsurgery mainly comes from ultramicroscope about the understanding of biological cell structure, ultramicroscope has very high space resolution capability, it has been widely used in medical science and biosphere, its resolution capability can reach 1.0nm (10 ), but biological sample needs through dehydration during the ultramicroscope imaging, section, dyeing and fixing etc., the part-structure that causes in preparation process disintegrates and environmental change, the non-former state of sample, optical microscope can be observed biological sample alive, but its space resolution capability is lower, according to Rayleigh criterion, the explanation limit of optical microscope is ε=0.61 λ/A, wherein λ is an incident wavelength, and A is the numerical aperture of optical microscope, and obviously the resolution limit and lambda1-wavelength are directly proportional, the explanation limit of optical microscope is 200.0 μ m, can not be used to observe cell and the subcellular structure of size in micron.In addition, cell can't carry out optical treatment for moving rapidly, can't effectively control the photoirradiation dosage that biological sample absorbs, and electric filament lamp cooperates suitable optical element also to bring the chromatic aberration of instrument, seriously influenced optical processing and observation targeted biological specimen.
Goal of the invention of the present utility model provides a kind of high spatial resolution that has, and cell is without prejudice, and has monochromaticity-promptly the remove microsurgery laser microbeam instrument of chromatic aberration.
The solution that is implemented in goal of the invention of the present utility model is as follows, comprise solid state laser (Nd:YAG), this laser instrument connects with Laser Power Devices, connect with the laser instrument control panel again, adjusting Q crystal in the laser instrument connects with transferring the Q circuit, key is the laser instrument head through light path over against-45 ° of 1064nm 532nm full impregnated diaphragm that is all-trans, long-focus lens and total reflection diaphragm, be provided with microscope again on the total reflection diaphragm light path, be provided with a light absorption tube on the diaphragm-operated reflected light path of full impregnated, the laser instrument head is provided with cooling-cycle device.Related circuit in this utility model, as Laser Power Devices, the control circuit of power supply transfers Q circuit and control panel to be existing circuit engineering.
This utility model is owing to use laser instrument, and uses short pulse, and so pulse length≤10ns is can be with its microbeam irradiation cell rapidly that moves.Because lambda1-wavelength is 532nm, so the ability height is divided in the space.Can observe element distribute, in microsurgery laser microbeam instrument microscope part, the absorption of incident laser can be used as a kind of detection means, the absorption of every bit can be calculated by the absorptance of component in the sample, a kind of absorptance of element difference when photon energy is below or above the uptake zone is very big, because the just difference in brightness of the original of microscopic, if our observation is below or above the microscopic iage of uptake zone, just can obtain the difference of a special element, we can utilize different elements to receive the cross section has very big difference this point to strengthen difference between the different ingredients of biological cell.Because pulse brightness is minimum, can avoid the radiation damage of biological cell.The situation of contrast electric filament lamp, the laser microbeam instrument can control effectively to the photoirradiation dosage that biological sample absorbs, and simultaneously because electric filament lamp comprises a lot of wavelength, and one of characteristic of laser is coherence and monochromaticity.Therefore the light that uses specific wavelength can compare the result of the light of laser result and other wavelength mutually, and brought the monochromaticity benefit aspect microsurgery, has eliminated the chromatic aberration of instrument, avoid that chromatic aberration brings have a strong impact on.
Provide the structure of utility model in detail below in conjunction with accompanying drawing.
Fig. 1 is a structural representation of the present utility model.
Fig. 2 is the structural representation of embodiment of the present utility model.
Please join Fig. 1 and Fig. 2, the structure of embodiment of the present utility model is as follows, comprise solid state laser (Nd:YAG), this laser head partly adopts the planoconvex unsteady cavity, concrete structure is the 1064nm total reflection diaphragm 1 of φ 30 of arranging continuously on light path, its radius of curvature is 1 meter, and KDP adjusting Q crystal 2 also is connected the Nd of Brewster gusset plate 3, variable aperture aperture 4, φ 5 * 80mm with transferring Q circuit 11
3+: YAG rod 5, φ's 30 is 50% output film 5, KTP frequency-doubling crystal 7 and the xenon flash lamp of being located at the φ 6 * 80mm that swashs torch 5 one sides at the 1064nm reflectance, and wherein said accent Q circuit is for moving back pressure type crystal switch circuit.The laser that laser instrument produces enters the total reflection diaphragm 10 of the 532nm of the φ 30 long focus lens 9 of be all-trans 532nm full impregnated diaphragm 8, focal length 2m of 45 ° of 1064nm and φ 30 through light path, enter the microscope on the reflected light path of being located at total reflection diaphragm 10 at last, the reflected light path of full impregnated diaphragm 8 is provided with light absorption tube 13.
Microscopical structural order recited above is, comprise aperture-variable diaphragm 14, the dichroic reflector diaphragm 15 that the following visible light wave range of 6500 is all-trans, eyepiece 16, diameter are that the following visible light wave range transmitance of restriction medium holes 17,6500 of 50 μ m is 0.03% negative sense field lens diaphragm 18 and object lens 19, are provided with home-made TDJ on the reflected light path of dichroic reflector diaphragm 15
6The type internal focusing telescope, it is adjustable in 1 μ m-2 mu m range that the microbeam instrument focuses on the laser spot diameter of exporting like this, can export laser spot diameter by the aperture that changes aperture-variable diaphragm.
The Laser Power Devices of above-mentioned present technology directly are connected with laser instrument with control panel, for further improving effect of the present utility model, photoirradiation dosage is control effectively, and Laser Power Devices are connected with permutator with two shunt capacitances respectively, but permutator opening and closing two relay J
1And J
2The laser facula energy density level that can carry out reirradiation and selected output like this is at 0.01J/mm
2-20J/mm
2And 0.01J/mm
2-64J/mm
2Adjustable in the dose double scope, can select repetition rate as required, as 1-10 time/second or manual single, as the be unstained nuclear of cell of repetitive irradiation, can make cell be subjected to irreversible injury, can give the cell enucleation with laser effectively, even not discharge under the nuclear situation also passable.
Laser bar 5 both sides of described laser instrument are provided with cools off the device of hesitating, and this device comprises compressor 20, radiator 21 and refrigerator 22, and refrigerator 22 connects laser instrument, connects laser instrument through Laser Power Devices hydraulic pressure protection port 24 again through water pump 23 in addition.
Claims (5)
1, a kind of microsurgery laser microbeam instrument, comprise solid state laser (Nd:YAG), this laser instrument connects with Laser Power Devices and connects with the laser instrument control panel, adjusting Q crystal in the laser instrument connects with transferring the Q circuit, it is characterized in that the laser instrument head through light path over against one 45 ° of 1064nm 532nm full impregnated diaphragm that is all-trans, long-focus lens and total reflection film are provided with microscope on the total reflection diaphragm light path again, be provided with an extinction tube on the diaphragm-operated reflected light path of full impregnated, the laser instrument head is provided with cooling-cycle device.
2, by the described microbeam instrument of claim 1, it is characterized in that described laser head partly adopts the planoconvex unsteady cavity, concrete structure be on light path, arrange continuously φ 30 1064nm total reflection diaphragm, KDP adjusting Q crystal and with transfer that the Q circuit is connected, the Nd of Brewster gusset plate, variable aperture aperture, φ 5 * 80mm
3+: YAG rod, φ's 30 is 50% output film at the 1064nm reflectance, KTP frequency-doubling crystal and be located at the xenon flash lamp of the φ 6 * 80mm of laser bar one side.
3, by the described microbeam instrument of claim 1, it is characterized in that dichroic reflector diaphragm, eyepiece, diameter that described microscopical structural order comprises that aperture-variable diaphragm, the following visible light wave range of 6500 are all-trans are that the following visible light wave range transmitance of restriction medium holes, 6500 of 50 μ m is 0.03% negative sense field lens diaphragm and object lens, are provided with home-made TDJ on the diaphragm-operated reflected light path of dichroic reflector
6The type internal focusing telescope.
4, by the described microbeam instrument of claim 1, it is characterized in that Laser Power Devices are connected with permutator with two shunt capacitances respectively, but permutator opening and closing two relay J
1And J
2
5, by the described microbeam instrument of claim 1; the both sides that it is characterized in that described laser bar are provided with cooling-cycle device; this device comprises compressor, radiator and refrigerator, and refrigerator connects laser instrument, connects laser instrument through the hydraulic pressure protection port of Laser Power Devices again through water pump in addition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98251839U CN2350006Y (en) | 1998-12-18 | 1998-12-18 | Laser microbeam apparatus for microsurgery |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98251839U CN2350006Y (en) | 1998-12-18 | 1998-12-18 | Laser microbeam apparatus for microsurgery |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2350006Y true CN2350006Y (en) | 1999-11-24 |
Family
ID=33995667
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN98251839U Expired - Fee Related CN2350006Y (en) | 1998-12-18 | 1998-12-18 | Laser microbeam apparatus for microsurgery |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2350006Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1842724B (en) * | 2003-08-25 | 2010-04-28 | 皇家飞利浦电子股份有限公司 | Emission Apodization Control for Microbeamformers |
-
1998
- 1998-12-18 CN CN98251839U patent/CN2350006Y/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1842724B (en) * | 2003-08-25 | 2010-04-28 | 皇家飞利浦电子股份有限公司 | Emission Apodization Control for Microbeamformers |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| C53 | Correction of patent for invention or patent application | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: PATENTEE ADDRESS; FROM: 230031 HEFEI CITY, ANHUI PROVINCE 1125 MAILBOX TO: ROOM #, TIWAIFANBO, LUOYANGYIZHUANFUSHUYI YARD, LUOYANG CITY, HENAN PROVINCE, 471003 |
|
| CP03 | Change of name, title or address |
Address after: 471003 Henan city of Luoyang province Luoyang Medical College Affiliated Hospital of external counterpulsation room Guo Juhua Patentee after: Zhao Ke Address before: 1125 mailbox 230031, Anhui City, Hefei Province Patentee before: Zhao Ke |
|
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |