CN211800903U - A centrifugal force microfluidic chip that can realize automatic sealing of liquid by balls - Google Patents

Abstract

The invention provides a centrifugal force microfluidic chip capable of automatically blocking liquid with balls, so as to solve the problem of whether the liquid channel is blocked by balls during sample detection. Existing in the liquid storage cavity, it is convenient to realize the biochemical reaction; when the balls do not block the liquid channel, the liquid storage cavity is communicated with the liquid channel, which is convenient to discharge the liquid in the liquid storage cavity by centrifugal force.

Description

A centrifugal force microfluidic chip that can realize automatic sealing of liquid by balls

Technical field:

The invention relates to the field of sample detection and the field of microfluidic chips, in particular to a spherical bead automatic plugging liquid structure used for sample detection in a microfluidic chip.

Background technique:

Lab-on-a-chip or microfluidic chip technology is a technology that integrates the processing, analysis and detection of chemical and biological samples into a micron-scale fluid chip, forming a network of microfluidic channels that can control the fluid throughout the entire system. Low-cost, fast, efficient, and high-throughput analysis and detection is an important information acquisition and processing platform for modern biological and chemical sciences. Microfluidic chips can not only complete automated operations, detection and analysis in traditional chemistry and biology, but also successfully implement certain experiments that are difficult or impossible to complete under traditional biological and chemical methods. Microfluidic chip technology has been widely used in pharmaceuticals, clinical diagnosis, food testing, environmental testing and other fields due to its advantages of flexible combination and scale integration of various unit technologies on an overall controllable micro-platform.

Invention content:

The invention provides a structure for realizing the automatic blocking of liquid by balls, so as to solve the problem of switching between the state of whether the liquid channel is blocked by the ball in the sample detection, and in the state of blocking the liquid channel by the ball, the reactant exists in the liquid storage cavity It is convenient to realize the biochemical reaction; in the state that the liquid channel is not blocked by the ball, the liquid storage cavity is communicated with the liquid channel, which is convenient to discharge the liquid in the liquid storage cavity by centrifugal force.

The centrifugal force microfluidic chip provided by the invention is mainly composed of a chip body, a rotating shaft and a ball, and a liquid storage cavity and a liquid channel are arranged on the chip body.

In the present invention, the rotating shaft and the chip body are fixed by the fixing device. In order to realize the switching of the ball blocking channel, the rotating shaft rotates clockwise, and the instantaneous acceleration makes the ball move to the position of the arc-shaped concave surface B in the liquid storage cavity. The channel is closed; the rotating shaft rotates counterclockwise, and the instantaneous acceleration causes the ball to move to the position of the arc-shaped concave surface A in the liquid storage chamber, and the channel is in an open state at this time; by switching the rotation direction of the rotating shaft, the ball is completed to block the liquid channel on and off.

The advantages of the present invention are that the production process of the present invention is relatively simple, the conversion speed is fast, and the portable control of the samples on the chip body can be realized.

Description of drawings:

The accompanying drawings are used to provide a further understanding of the present invention, and constitute a part of the specification, and are used to explain the present invention together with the embodiments of the present invention, and do not constitute a limitation to the present invention. In the attached image:

1. Figure 1 is a schematic plan view of the automatic blocking structure of balls. In the figure, 1 represents the rotating shaft, 2 represents the liquid storage chamber, the liquid storage chamber has two arc-shaped concave structures A and B, and 3 represents the ball used for blocking the liquid. , the ball is located in the liquid storage chamber, and the current ball is in the arc concave A position (channel closed).

2. Figure 2 is a schematic side view of the automatic blocking structure of the ball. In the figure, 1 represents the rotating shaft, 2 represents the liquid storage chamber and the two arc-shaped concave structures of A and B, and 3 represents the ball used for blocking the liquid. In the liquid storage chamber, the current ball is in the arc-shaped concave surface A position (the channel is closed), and 4 represents the liquid channel connected to the liquid storage chamber.

3. Figure 3 is a schematic diagram 1/2 of the application of the ball automatic plugging structure. In the figure, 1 represents the rotating shaft, 2 represents the liquid storage chamber, the liquid storage chamber has two arc-shaped concave structures A and B, and 3 represents the plugging liquid. The current ball is in the arc-shaped concave surface A position (the channel is closed), 4 represents the liquid channel connecting the liquid storage chamber and the detection chamber, and 5 represents the detection chamber. In this schematic state, the liquid in the liquid storage chamber cannot enter the detection chamber through the liquid channel by centrifugal force.

4. Figure 4 is a schematic diagram 2/2 of the application of the ball automatic blocking structure. The current ball is in the arc-shaped concave surface B position (the channel is open). In this schematic state, the liquid in the liquid storage cavity can be detected by centrifugal force through the liquid channel. cavity.

Detailed ways

The preferred embodiments of the present invention will be described below with reference to the accompanying drawings. It should be understood that the embodiments described herein are only used to illustrate and explain the present invention, but not to limit the present invention.

A simple liquid centrifugal force microfluidic chip structure is automatically blocked by balls, comprising a chip body, a chip rotating shaft and a ball, and a liquid storage cavity and a liquid channel are arranged on the chip body. The specific structure diagram is shown in FIG. 1 and FIG. 2 , and the specific implementation manner is shown in FIG. 3 and FIG. 4 . Structure 1 is a rotating shaft, which is connected with a motor to control the rotation speed and direction of the chip, and is fixed with the chip body. Structure 2 is a liquid storage cavity located on the chip body, with two arc-shaped concave structures A and B. The structure 3 is a ball used to block the liquid, which is stored in the liquid storage cavity, and the structure 4 is a liquid channel connecting the liquid storage cavity and the detection cavity. Structure 5 represents the detection chamber, which is connected to the liquid channel.

Concretely realize the operation process of ball-blocking liquid: it is assumed that there is reaction liquid in the liquid storage chamber.

1. When the liquid in the liquid storage chamber is incubating, a counterclockwise rotational acceleration is given to the chip body by rotating the rotating shaft, and the chip body rotates with the rotating shaft. At the same time, the beads enter the arc-shaped concave surface A area of the liquid storage chamber. , block the liquid channel connecting the liquid storage cavity and the detection cavity, and the liquid is limited in the liquid storage cavity at this time;

2. When the liquid in the liquid storage chamber needs to enter the detection chamber, change the direction of the acceleration of the rotating shaft, and give the chip body a clockwise rotational acceleration through the rotation of the rotating shaft. The chip body moves with the rotating shaft, and at the same time, the beads enter the liquid storage. The arc-shaped concave surface B area of the cavity is connected to the liquid channel connecting the liquid storage cavity and the detection cavity. At this time, the liquid will enter the detection cavity through the liquid channel after reaching a certain rotation speed with the increase of the rotation speed.

It can be seen from this that if you want to switch the liquid state of ball blocking in the liquid storage cavity, you only need to switch the rotation direction of the rotating shaft, which is convenient and quick, and can achieve multiple switching.

Claims (7)

1. The centrifugal force micro-fluidic chip capable of achieving automatic liquid plugging of the round beads comprises a chip body, a rotating shaft and the round beads, wherein a liquid storage cavity and a liquid channel are formed in the chip body, the rotating shaft is perpendicular to the plane of the chip body, and the centrifugal force micro-fluidic chip is characterized in that one or more automatic ball plugging structures are further arranged on the chip body and comprise the round beads and a 3-shaped structure, the 3-shaped structure is formed by 2 arcs in parallel and is in a cavity wall with the shape like the number 3, the opening side of the 3-shaped structure faces towards the rotating shaft of the chip to form a cavity wall on the far rotating shaft side of the liquid storage cavity, a small hole is formed in the arc top position of one arc concave surface A and communicated with the liquid channel, a small hole is not formed in the arc top position of the other arc concave surface B, and the round beads can be used for plugging the 2 arc concave surfaces A and A of the 3-, And B, moving, blocking the liquid channel when the ball is positioned in the arc-shaped concave surface A, and opening the liquid channel when the ball is positioned in the arc-shaped concave surface B.

2. The centrifugal force microfluidic chip capable of automatically plugging liquid by using the ball according to claim 1, wherein the surface of the ball is coated with a rubber material.

3. The centrifugal force microfluidic chip capable of automatically plugging liquid by using the ball according to claim 1, wherein the small hole arranged at the arc top position of the arc concave surface A is arranged in a funnel shape, and the ball enters the funnel-shaped structure to more tightly plug the liquid.

4. The centrifugal force microfluidic chip capable of achieving automatic liquid plugging by the ball according to claim 1, 2 or 3, wherein the ball is made of magnetic metal and can move in a 3-shaped structure under the control of a magnetic field.

5. The centrifugal force microfluidic chip capable of automatically plugging liquid by using the ball according to claim 4, wherein the ball is made of an iron alloy, iron, cobalt, nickel or gadolinium.

6. The centrifugal force microfluidic chip capable of achieving automatic ball plugging of liquid according to claim 4, wherein an independent magnet is arranged above or below the chip, and the ball is guided by the magnet to move, so that the ball automatic plugging structure is opened and closed.

7. The centrifugal force microfluidic chip capable of achieving automatic ball plugging of liquid according to claim 5, wherein an independent magnet is arranged above or below the chip, and the ball is guided by the magnet to move, so that the ball automatic plugging structure is opened and closed.

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