CN211339466U - A New Type of PCR Amplifier - Google Patents

Abstract

The utility model discloses a novel PCR amplification instrument, comprising an amplification instrument body, the bottom surface of the shell of the amplification instrument body is a support table and an air inlet area, the support table is fixedly connected with a pillar, and the lower end of the pillar is fixed A foot pad is connected, the air inlet area is provided with an elliptical air inlet, the back of the casing is provided with a pyramidal air outlet, the surface of the casing is covered with a thermal insulation layer, and the rear side wall of the amplification instrument body is provided. With test tube rack. The utility model fixes the foot pad on the workbench, so that the amplification instrument body is fixed on the workbench, and the stability of the instrument is enhanced; the pillar supports the bottom surface of the instrument, so that the air intake volume is larger, and the air outlet has a pyramid-shaped structure. Make the air outlet faster, so that the annealing efficiency of the instrument is better; the thermal insulation layer of the outer shell effectively makes the instrument not affected by the ambient temperature, making the internal temperature control of the instrument more accurate; the test tube rack on the side wall of the amplification instrument body can be easily taken Take test tubes to improve work efficiency.

Description

A New Type of PCR Amplifier

technical field

The utility model relates to an experimental instrument in the field of biochemistry, in particular to a novel PCR amplification instrument.

Background technique

PCR (Polymerase Chain Reaction) (also known as: polymerase chain reaction) PCR is a method of enzymatic synthesis of specific DNA fragments in vitro. The reaction consists of a cycle, and the cycle is carried out, so that the target DNA can be rapidly amplified, and has the characteristics of strong specificity, high sensitivity, simple operation, and time saving. It can be used not only for basic research such as gene isolation, cloning and nucleic acid sequence analysis, but also for the diagnosis of diseases or anywhere there is DNA, RNA. PCR is also known as cell-free molecular cloning or in vitro primer-directed enzymatic amplification of specific DNA sequences.

During the PCR reaction, the module containing the sample needs to be rapidly heated and cooled, so as to achieve the purpose of denaturing the sample at high temperature and annealing at low temperature. In the process of low-temperature annealing, it is necessary to introduce external wind to cool the module. The traditional instrument is equipped with an air inlet at the bottom, but the installation of the foot pad is too high, which will cause the instability of the center of gravity of the gene amplification instrument, and the installation of the foot pad is too low, which will cause the air inlet to enter. If the air volume is too small, it is difficult to ensure the rapid cooling function of the gene amplification instrument. In addition, the existing instruments are generally placed on the workbench, which is easily affected by the external temperature. In addition, without safety protection, the accidental collision of the instrument during operation will affect the quality of the chain reaction; the test tube needs to be used or replaced during the test. When the test tube is not on the side, it is more troublesome to take.

Utility model content

In order to solve the above problems, the utility model proposes a new type of PCR amplifying instrument with anti-collision, good shock absorption effect, little influence by external temperature, rapid annealing and cooling, and convenient taking of test tubes.

The utility model adopts the following technical solutions: a novel PCR amplification instrument, which is characterized in that it comprises an amplification instrument body, the two ends of the bottom surface of the outer shell of the amplification instrument body are support platforms, and the middle of the bottom surface of the outer shell is an air inlet area , the support table is fixedly connected to the upper end of the pillar, the lower end of the pillar is fixedly connected with a foot pad, the foot pad is fixedly connected to the workbench, the air inlet area is provided with an air inlet, and the back of the shell is provided with an air outlet , the surface of the shell is covered with a thermal insulation layer, and a test tube rack is fixedly connected to the back end sidewall of the amplification instrument body.

Further, the support table has a rectangular structure, which makes the bottom of the instrument more stable when fixed on the workbench, and the air intake area is an arch bridge structure, which can make it easier for wind to enter the inside of the instrument.

Further, the pillars have a cylindrical structure, and there are four pillars, which are respectively located at both ends of the support table, and the pillars support the bottom surface, which can effectively increase the air intake volume.

Further, the foot pads are circular, there are positioning holes on the foot pads, the foot pads are fixedly connected to the workbench by screws through the positioning holes, and there are washers between the foot pads and the workbench. It mainly plays the role of fixing the instrument and the workbench.

Further, the positioning holes are circular, and there are four positioning holes.

Further, the washer is made of rubber material, and the washer has four holes corresponding to the positioning holes. The washer can reduce the friction between the foot pad and the workbench, and has a certain anti-vibration effect.

Further, the air inlet is located in the middle of the air inlet area, and the air inlet has an elliptical structure.

Further, the air outlet is a pyramid-shaped structure, and the pyramid-shaped outlet makes the air after passing through the instrument go out faster, increasing the internal heat exchange and cooling rate. Effectively reduce the influence of external temperature on the inside of the instrument.

Further, the test tube rack includes an upper test tube rack and a lower test tube rack, the upper test tube rack is located above the back-end sidewall of the amplification instrument body, and the lower test-tube rack is located below the back-end sidewall of the amplification instrument.

Further, the test tube rack has holes, the holes of the upper test tube rack are larger than the holes of the lower test tube rack, and the test tubes used for the reaction can be placed on the test tube rack, which is convenient to take and improves the work efficiency.

The beneficial effects of the present utility model are:

1. The utility model fixes the foot pad on the workbench through the positioning hole through the screw, so that the PCR amplifier is fixed on the workbench, which enhances the stability of the instrument and prevents the instrument from affecting the polymerization under the condition of accidental collision. The effect of the enzyme chain reaction.

2. The bottom surface of the instrument is supported by the pillars, which makes the air intake larger under the condition of ensuring the stable center of gravity of the instrument, and the pyramid-shaped structure of the air outlet makes the air out faster, so that the annealing efficiency of the instrument is better.

3. The thermal insulation layer of the shell can effectively make the instrument not affected by the ambient temperature, and make the internal temperature control system of the instrument work more efficiently.

4. The test tube rack on the side wall of the amplification instrument body can easily take the test tube and improve the work efficiency.

Description of drawings

Figure 1 is a schematic structural diagram of the utility model.

Figure 2 is a schematic diagram of the structure of the air inlet of the present invention.

FIG. 3 is a schematic diagram of the structure of the foot pad of the present invention.

FIG. 4 is a schematic diagram of the structure of the air outlet of the present invention.

Description of reference numbers:

10 Workbench, 20 Thermal expansion instrument body, 21 Shell, 22 Support table, 221 Pillar, 222 Foot pad, 2221 Positioning hole, 223 Washer, 23 Air inlet area, 231 Air inlet, 24 Test tube rack, 241 Upper test tube rack, 242 Lower the test tube rack, 25 air outlets.

Detailed ways

The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, but not all of them. example. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present invention.

Example 1

A novel PCR amplification instrument is characterized in that it includes an amplification instrument body 20, the two ends of the bottom surface of the outer shell 21 of the amplification instrument body 20 are support tables 22, and the middle of the bottom surface of the outer shell 21 is an air inlet area 23, so the The support table 22 is fixedly connected to the upper end of the pillar 221, the lower end of the pillar 221 is fixedly connected with a foot pad 222, the foot pad 222 is fixedly connected to the workbench 10, the air inlet area 23 is provided with an air inlet 231, the An air outlet 25 is provided on the back of the outer casing 21 , the surface of the outer casing 21 is covered with a heat insulating layer, and a test tube rack 24 is fixedly connected to the rear side wall of the amplification instrument body 20 .

The support table 22 is a rectangular structure, which makes the bottom of the instrument more stable when fixed on the workbench 10; the air inlet area 23 is an arch bridge structure, and the arch bridge structure can make it easier for the wind to enter the inside of the instrument; the pillar 221 is a cylindrical structure, and the pillar 221 There are four, which are located at both ends of the support table 22 respectively. The bottom surface is supported by the pillars 221, which can effectively increase the air intake. The foot pads 222 are circular, and the foot pads 222 are provided with positioning holes 2221. The foot pads 222 are positioned through screws. The hole 2221 is fixedly connected with the workbench 10, there is a washer 223 between the foot pad 222 and the workbench 10, the foot pad 222 mainly plays the role of fixing the instrument and the workbench 10; the positioning hole 2221 is circular, and there are four positioning holes 2221 The gasket 223 is a rubber material, and there are four holes corresponding to the positioning holes 2221 on the gasket 223. The gasket 223 can reduce the friction between the foot pad 222 and the workbench 10, and has a certain anti-vibration effect; the air inlet 231 is located in the air inlet area 23 In the middle, the air inlet 231 is an oval structure; the air outlet 25 is a pyramid-shaped structure, and the pyramid-shaped outlet makes the wind after passing through the instrument go out faster, increasing the internal heat exchange and cooling rate; the heat insulation layer on the surface of the shell 21 is silica gel The material, the heat insulation layer effectively reduces the influence of the external temperature on the inside of the amplification instrument body 20; Above, the lower test tube rack 242 is located below the back end side wall of the amplification instrument body 20; the test tube rack 24 has holes, the holes of the upper test tube rack 241 are larger than the holes of the lower test tube rack 242, and the test tubes for the reaction can be placed in the test tube rack 24, easy to take and improve work efficiency.

Example 2

The height of the pillar 221 is 2-3 cm, and the radius is 1.5 cm, which ensures that the bottom surface has a sufficient height from the workbench 10 and the bearing capacity of the pillar 221 for the instrument. The contact area of the table 10 is large enough, and the connection is stable enough when using screws. Another effect of using screws is that when the table 10 needs to be replaced together, the screws can be removed to fix the instrument to another table 10; 21 The thermal insulation layer covered on the surface can also be made of rubber material; when the instrument is working, if there is an accidental collision, the new model has good stability compared to the instrument not fixed on the workbench 10; in different seasons or day and night temperature differences In large cases, the effect of the thermal insulation layer can reduce the influence of the external temperature on the internal temperature control system and improve the working efficiency of the internal temperature control system; the pyramid-shaped structure of the air outlet 25 is more efficient than the square structure. On the premise of sufficient air intake, the annealing efficiency can be effectively improved; the diameter of the holes of the upper test tube rack 241 is 10 mm, and the diameter of the holes of the lower test tube rack 242 is 5 mm, and the diameter of the holes is larger than that of most test tubes. It is slightly larger, so it is suitable for the placement of most test tubes and has high versatility.

Claims (10)

1. The utility model provides a novel PCR amplifys appearance, a serial communication port, including augmenting appearance body (20), shell (21) bottom surface both ends of augmenting appearance body (20) are brace table (22), be air inlet district (23) in the middle of shell (21) bottom surface, brace table (22) and pillar (221) upper end fixed connection, pillar (221) lower extreme fixedly connected with callus on the sole (222), callus on the sole (222) fixed connection is on workstation (10), air inlet district (23) are equipped with air intake (231), shell (21) back is equipped with air outlet (25), shell (21) surface covers has the insulating layer, augment appearance body (20) rear end lateral wall fixedly connected with test-tube rack (24).

2. The PCR amplification apparatus of claim 1, wherein the support platform (22) is rectangular and the air inlet area (23) is arched.

3. The PCR amplification apparatus of claim 1, wherein the pillars (221) are cylindrical, and four of the pillars (221) are respectively located at two ends of the support platform (22).

4. The PCR amplification instrument of claim 1, wherein the foot pad (222) is circular, the foot pad (222) is provided with a positioning hole (2221), the foot pad (222) is fixedly connected with the worktable (10) through the positioning hole (2221) by a screw, and a gasket (223) is arranged between the foot pad (222) and the worktable (10).

5. The PCR amplification apparatus of claim 4, wherein the positioning holes (2221) are circular, and the number of the positioning holes (2221) is four.

6. The PCR amplification apparatus of claim 4, wherein the gasket (223) is made of rubber, and the gasket (223) has four holes corresponding to the positioning holes (2221).

7. The novel PCR amplification instrument as claimed in claim 1, wherein the air inlet (231) is located in the middle of the air inlet area (23), and the air inlet (231) has an oval structure.

8. The PCR amplification apparatus as claimed in claim 1, wherein the air outlet (25) has a pyramid structure, and the heat insulation layer on the surface of the housing (21) is made of silica gel.

9. The PCR amplification apparatus as claimed in claim 1, wherein the test tube rack (24) comprises an upper test tube rack (241) and a lower test tube rack (242), the upper test tube rack (241) is located above the rear end side wall of the amplification apparatus body (20), and the lower test tube rack (242) is located below the rear end side wall of the amplification apparatus body (20).

10. The PCR amplification apparatus of claim 9, wherein the test tube rack (24) has holes, and the holes of the upper test tube rack (241) are larger than the holes of the lower test tube rack (242).

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