CN210736776U - a culture detector - Google Patents

Abstract

The utility model relates to the technical field of detectors, in particular to a culture detector, which comprises a protective shell, a cylinder, a manipulator, an incubator, a control system and a fluorescence detection system, wherein the incubator is provided with a plurality of accommodating cavities, culture dishes are arranged in the accommodating cavities, compared with the traditional detector, the incubator is added, the culture dishes can be placed in the accommodating cavities during the culture period through the manipulator, the utility model can accommodate a plurality of culture dishes, the fluorescence detection system can detect the strains with 6PD-DPAN fluorescence probes in the culture dishes, and send the detected data to the control system for processing, the control system can also control the manipulator and the incubator, the utility model can dynamically track and monitor the dynamic process of the combination of the 6PD-DPAN fluorescence probes and the strains in real time, the method realizes rapid and accurate detection of the bacteria medicament and has high practicability.

Description

a culture detector

technical field

The utility model relates to the technical field of detectors, in particular to a culture detector.

Background technique

Antimicrobial susceptibility testing (AST) is an important means to provide information on the in vitro susceptibility of clinical pathogens to different antibacterial drugs. However, the traditional bacterial susceptibility testing (AST) is a growth-based susceptibility testing method, which relies on the growth of microorganisms. Therefore, it takes 24-48 hours or longer to reach the level that can be distinguished by visual inspection or instruments. However, it is difficult for strains to correctly and reproducibly determine the growth focus. In addition, the results are highly subjective, the MIC value cannot be measured (disc diffusion method), and the applicable drugs and strains are limited, which cannot bring timely clinical results. The feedback from the results not only affects the precise medication of critically ill patients, but also greatly affects the clinical value of drug susceptibility testing.

The existing detection system has a complex structure and can only detect one petri dish. It takes a long time to detect multiple petri dishes. In addition, the existing detector has no incubator, and the detection process requires manual intervention, which cannot be realized. Automated detection.

SUMMARY OF THE INVENTION

Aiming at the problems of the prior art, the utility model provides a culture detector with an incubator that can be automatically detected.

In order to solve the above-mentioned technical problems, the utility model adopts the following technical solutions:

A culture detector provided by the utility model comprises a protective shell, a cylinder, a manipulator, an incubator, a control system and a fluorescence detection system. One end of the protective shell is provided with a fixed shaft, and the manipulator is slidably arranged on the fixed shaft. On the shaft, one end of the manipulator is connected to the cylinder, the incubator is installed on the other end of the protective shell, the incubator is provided with several accommodating cavities, and a culture dish is installed in the accommodating cavity, and the The fluorescence detection system is arranged above the manipulator, the control system is electrically connected with the cylinder and the fluorescence detection system respectively, and an observation window is arranged at the front end of the protective shell.

Preferably, the culture detector further includes a temperature controller and a humidity controller, and both the temperature controller and the humidity controller are electrically connected to the control system.

Preferably, the culture detector further includes a display screen and an indicator light, the indicator light and the display screen are both installed outside the protective shell, and the indicator light and the display screen are both electrically connected to the control system. sexual connection.

Preferably, the control system includes a signal receiving unit, an amplifier, a processor, a data processing unit and a data sending unit, the signal receiving unit is electrically connected to the processor through the amplifier, and the processor is connected to the The data processing unit is electrically connected, and the data processing unit is electrically connected with the data sending unit.

Preferably, the fluorescence detection system includes a light source emitter for emitting a detection light source, an excitation light filter for filtering out the stray light of the excitation light source emitted by the light source emitter, and an excitation light filter for filtering out the emission of the sample to be tested. A fluorescent filter for stray light in a fluorescent light source, a detector for detecting the intensity of the fluorescent light source, a controller for regulating detection conditions and controlling the work of the light source emitter and the detector.

Preferably, a plurality of accommodating cavities are arranged in a row, and a partition plate is arranged between two adjacent accommodating cavities, and the partition plate is provided with a space-apart portion.

Preferably, the culture detector further includes a sterilization system, and the sterilization system is electrically connected to the control system.

Preferably, the upper part of the culture dish has a sterile 96-well plate with multiple rows of concave holes.

The beneficial effects of the present utility model:

A culture detector provided by the utility model comprises a protective shell, a cylinder, a manipulator, an incubator, a control system and a fluorescence detection system. One end of the protective shell is provided with a fixed shaft, and the manipulator is slidably arranged on the fixed shaft. On the shaft, one end of the manipulator is connected to the cylinder, the incubator is installed on the other end of the protective shell, the incubator is provided with several accommodating cavities, and a culture dish is installed in the accommodating cavity, and the The fluorescence detection system is arranged above the manipulator, the control system is electrically connected with the cylinder and the fluorescence detection system respectively, and the front end of the protective shell is provided with an observation window. Compared with the traditional detector, the utility model has An incubator is added, and the petri dish can be placed in the accommodating cavity during the incubation period through a manipulator. The utility model can accommodate multiple petri dishes, and the fluorescence detection system of the present invention can monitor the 6PD-DPAN fluorescent probe in the petri dish. The strain of the needle is detected, and the detected data is sent to the control system for processing. The control system can also control the manipulator and the incubator. The utility model can dynamically track and monitor the combination of the 6PD-DPAN fluorescent probe and the strain in real time. In addition, the utility model is provided with an observation window, through which the user can observe the running process of the culture detector in real time.

Description of drawings

Figure 1 is a schematic structural diagram of the utility model.

Figure 2 is a cross-sectional view of the utility model.

FIG. 3 is a schematic diagram of the petri dish of the present invention.

FIG. 4 is a frame diagram of the control principle of the present invention.

The reference numbers are:

Protective shell--1, cylinder--2, manipulator--3, incubator--4, control system--5, fluorescence detection system--6, observation window--7, temperature controller--8, humidity control device--9, display screen--10, indicator light--11, signal receiving unit--12, amplifier--13, processor--14, data processing unit--15, data sending unit--16, light source Emitter--17, Excitation Filter--18, Fluorescence Filter--19, Detector--20, Controller--21, Sterilization System--22, Petri Dish--23, Separator- -twenty four.

Detailed ways

In order to facilitate the understanding of those skilled in the art, the present invention will be further described below with reference to the embodiments and the accompanying drawings, and the contents mentioned in the embodiments are not intended to limit the present invention. The present utility model will be described in detail below with reference to the accompanying drawings.

As shown in Figures 1-4, a culture detector provided by the present invention includes a protective shell 1, a cylinder 2, a manipulator 3, an incubator 4, a control system 5 and a fluorescence detection system 6. One end is provided with a fixed shaft, the manipulator 3 is slidably arranged on the fixed shaft, one end of the manipulator 3 is connected with the cylinder 2, the incubator 4 is installed on the other end of the protective shell 1, and the cultivation The container 4 is provided with a number of accommodating cavities, in which a culture dish 23 is installed, the fluorescence detection system 6 is arranged above the manipulator 3, and the control system 5 is respectively connected with the cylinder 2 and the fluorescence detection system. The system 6 is electrically connected, and the front end of the protective shell 1 is provided with an observation window 7. Compared with the traditional detector, the present invention adds an incubator 4, and the petri dish 23 can be placed in the accommodating cavity during the incubation period through the manipulator 3 , the present invention can accommodate multiple petri dishes 23, and the fluorescence detection system 6 of the present invention can detect the strains with monitoring 6PD-DPAN fluorescent probes in the petri dish 23, and send the detected data to the control The system 5 performs processing, and the control system 5 can also control the manipulator 3 and the incubator 4. The utility model can dynamically track and monitor the kinetic process of the combination of the 6PD-DPAN fluorescent probe and the strain in real time, so as to realize fast and accurate bacterial medicine. The utility model is provided with an observation window 7, and the user can observe the operation process of the culture detector in real time through the observation window 7.

Specifically, during detection, the manipulator 3 can take out the petri dish 23 and place it under the fluorescence detection system 6 for detection.

In this embodiment, the culture detector further includes a temperature controller 8 and a humidity controller 9. Both the temperature controller 8 and the humidity controller 9 are electrically connected to the control system 5. The temperature controller is used for Detect and control the temperature in the protective shell 1, and the humidity controller is used to detect and control the humidity in the protective shell 1. Specifically, a temperature controller and a humidity controller can be used to detect the temperature and humidity. The temperature and humidity inside the protective case 1 are adjusted.

In this embodiment, the culture detector further includes a display screen 10 and an indicator light 11, the indicator light 11 and the display screen 10 are both installed outside the protective shell 1, the indicator light 11, the display The screens 10 are all electrically connected to the control system 5 .

In this embodiment, the control system 5 includes a signal receiving unit 12 , an amplifier 13 , a processor 14 , a data processing unit 15 and a data sending unit 16 , and the signal receiving unit 12 communicates with the processor 14 through the amplifier 13 . For electrical connection, the processor 14 is electrically connected to the data processing unit 15 , and the data processing unit 15 is electrically connected to the data sending unit 16 .

In this embodiment, the fluorescence detection system 6 includes a light source emitter 17 for emitting a detection light source, an excitation light filter 18 for filtering out the stray light of the excitation light source emitted by the light source emitter 17, and an excitation light filter 18 for filtering The fluorescent filter 19 for removing stray light in the fluorescent light source emitted by the sample to be tested, the detector 20 for detecting the intensity of the fluorescent light source, the controller 21 for regulating the detection conditions and controlling the work of the light source emitter 17 and the detector 20, Its working process is as follows: first, several strains with 6PD-DPAN fluorescent probes are placed on several petri dishes 23, and several petri dishes 23 are placed in the accommodating cavity of the incubator 4 by the manipulator 3, and then the control system 5 Adjust the detection conditions, start the light source emitter 17 to emit a laser light source, and the laser light source enters the sample to be tested containing the 6PD-DPAN fluorescent probe after passing through the excitation light filter 18. Under the irradiation of the excitation light, the 6PD-DPAN fluorescent probe The fluorescent light source is emitted, and the emitted fluorescent light source is filtered by the fluorescent filter 19 to filter out the stray light in the fluorescent light source, and then enters the detector 20 to detect the intensity of the fluorescent light source, and the detector 20 outputs the signal to control the signal of the system 5 The receiving unit 12 receives the signal output by the detector, and the received signal is amplified by the amplifier 13 and then sent to the processor 14. The processor 14 sends the received signal to the data processing unit 15, and the data processing unit 15 analyzes and analyzes the data. After processing, the analyzed and processed signal is sent to the display screen 10 through the data sending unit 16. The entire detection process is convenient and fast, with high detection sensitivity and high accuracy, and the 6PD-DPAN fluorescent probe can be monitored in real time through changes in fluorescence intensity. The kinetic process of strain binding, and the process of action on the strain.

In this embodiment, several accommodating cavities are arranged in a row, and a partition 24 is arranged between two adjacent accommodating cavities. The partition 24 is provided with a space-apart portion, and the partition 24 is used to place the culture dish 23 to allow The position is convenient for the manipulator 3 to grip the culture dish 23 .

In this embodiment, the culture detector further includes a disinfection system 22, and the disinfection system 22 is electrically connected to the control system 5. After the detection incubator is used up, the control system 5 can control the disinfection system 22 to perform the detection The incubator is sterilized, and the sterilization system 22 may be ultraviolet sterilization and high temperature sterilization.

In this embodiment, the upper part of the culture dish 23 has a sterile 96-well plate with multiple rows of concave holes. The sterile 96-well plate can accommodate multiple strains at the same time, and can quickly cultivate multiple strains. and detection.

In this embodiment, the strain to be tested with 6PD-DPAN fluorescent probe is prepared by the following steps:

Step (1): the strain to be tested is configured to a bacterial suspension with a McFarland turbidity of 0.5 with physiological saline;

Step (2): Dilute 1 mL of 6PD-DPAN fluorescent probe and 10 μL of the bacterial suspension in step (1) into 10 mL of MOPS buffer, shake well to prepare a mixed solution;

Step (3): Add 50 μL of the mixture prepared in step (2) to each well of the sterile disposable 96-well plate, and then add 50 μL of the mixture to the first well of the sterile disposable 96-well plate. MOPS buffer was used to prepare a control sample, and 50 μL of different concentrations of antibacterial drug solutions were added to each well except the first well to prepare several samples to be tested, and the plate was sealed with a transparent film to detect and record 6PD - The initial fluorescence intensity value of the DPAN fluorescent probe combined with the strain in the mixture;

Step (4): The above-mentioned disposable sterile 96-well plate with the sample to be tested is incubated in an incubation device with a constant temperature of 35°C, and every 15 minutes of incubation, the disposable sterile 96-well plate is taken out of the incubation device and placed in the incubation device. Place it in a fluorescence detection device to detect and record the fluorescence intensity value of the sample to be tested.

The above are only preferred embodiments of the present invention, and are not intended to limit the present invention in any form. Although the present invention is disclosed as a preferred embodiment, it is not intended to limit the present Professional and technical personnel, within the scope of the technical solution of the present invention, make some changes or modifications to the equivalent embodiments of the equivalent changes by using the technical content disclosed above, provided that the content of the technical solution of the present invention is not departed from, according to the present invention. The utility model technology refers to any simple modification, equivalent change and modification made to the above embodiments, which all belong to the scope of the technical solution of the present utility model.

Claims (8)

1. A culture detector is characterized in that: including protective housing, cylinder, manipulator, incubator, control system and fluorescence detection system, the inside one end of protective housing is provided with the fixed axle, the manipulator slide set up in on the fixed axle, manipulator one end with the cylinder is connected, the incubator install in the other end of protective housing, the incubator is provided with a plurality of and holds the chamber, it installs the culture dish to hold the intracavity, fluorescence detection system set up in the manipulator, control system respectively with the cylinder fluorescence detection system electric connection, the protective housing front end is provided with the observation window.

2. The culture detector of claim 1, wherein: the cultivation detector further comprises a temperature controller and a humidity controller, and the temperature controller and the humidity controller are electrically connected with the control system.

3. The culture detector of claim 1, wherein: cultivate the detector still includes display screen and pilot lamp, the pilot lamp the display screen all install in the protective housing is outside, the pilot lamp the display screen all with control system electric connection.

4. The culture detector of claim 1, wherein: the control system comprises a signal receiving unit, an amplifier, a processor, a data processing unit and a data sending unit, wherein the signal receiving unit is electrically connected with the processor through the amplifier, the processor is electrically connected with the data processing unit, and the data processing unit is electrically connected with the data sending unit.

5. The culture detector of claim 1, wherein: the fluorescence detection system comprises a light source emitter for emitting a detection light source, an exciting light filter for filtering stray light of an exciting light source emitted by the light source emitter, a fluorescence filter for filtering stray light in a fluorescence light source emitted by a sample to be detected, a detector for detecting the intensity of the fluorescence light source, and a controller for regulating and controlling detection conditions and controlling the light source emitter and the detector to work.

6. The culture detector of claim 1, wherein: the plurality of holds the chamber and is listed as the distribution, and adjacent two are held and are provided with the baffle between the chamber, be equipped with on the baffle and let the position portion.

7. The culture detector of claim 1, wherein: the culture detector further comprises a disinfection system, and the disinfection system is electrically connected with the control system.

8. The culture detector of claim 1, wherein: the upper part of the culture dish is provided with a sterile 96-well plate with a plurality of rows of concave holes.

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