CN216899874U

CN216899874U - Micro-observation lining plate

Info

Publication number: CN216899874U
Application number: CN202122624843.7U
Authority: CN
Inventors: 楼伟; 夏颖伟
Original assignee: SHANGHAI ANJIU BIOTECHNOLOGY CO Ltd
Current assignee: SHANGHAI ANJIU BIOTECHNOLOGY CO Ltd
Priority date: 2021-10-29
Filing date: 2021-10-29
Publication date: 2022-07-05
Anticipated expiration: 2031-10-29

Abstract

The application discloses a microscopic observation lining plate, which comprises a culture dish placing area and a frozen biological sample carrier placing area, wherein the culture dish placing area is provided with one or more culture dish placing positions; the frozen biological sample carrier placing area is provided with one or more carrier placing positions; the height of the place where the culture dish is placed and the height of the place where the carrier is placed form the same focusing distance with respect to the microscope. This application makes the culture dish sample that needs to observe be raised to placing the same height in district with the carrier, easily uses same focus to observe, need not to observe and readjust microscope focus for different regions, is applicable to the examination of embryo cell in batches, labour saving and time saving.

Description

Micro-observation lining plate

Technical Field

The utility model relates to the field of biological sample freezing devices, in particular to a biological sample vitrification freezing carrier and a microscopic observation lining plate matched with the same.

Background

In the artificial assisted reproduction technology, the ovum or a plurality of embryos obtained after in vitro fertilization are put into liquid nitrogen with the temperature of 196 ℃ below zero for freezing storage for short-term or long-term phase-selective embryo transplantation, and the method becomes a common method for knowing the infertility. The clinical significance of the vitrified frozen biological sample is that the vitrified frozen biological sample can be stored in a coolant such as liquid nitrogen for a long time, embryos or cells frozen by the coolant stop developing and can be stored for decades, and secondly, the frozen biological sample can revive the embryos or cells in the biological significance after being rewarming.

When the normal temperature material is frozen, a Leidenfrost effect generated when the normal temperature material meets a cryogenic coolant such as liquid nitrogen can form a heat-conduction-blocking vapor film on the surface of the frozen material and slow down the cooling speed of the material to be frozen, and the formation of ice crystallization in a biological sample in the freezing process leads to the reduction of the revival rate of the recovered biological sample, so that a vitrification freezing technology pursuing rapid freezing is developed.

At present, in order to accelerate the freezing rate, the vitrification freezing carrier at home and abroad is generally in an open type, such as a thinning wheat tube method, a quartz capillary tube method, a Cryoloop method, a Cryotip method and the like, namely, the freezing carrier carrying the sample is directly immersed in liquid nitrogen. In 2005, Kuwayama proposed a new method of increasing the cooling rate by minimizing the volume of the solution-Cryotop method, etc., which involves minimizing the volume of cryoprotectant, i.e., immersing the sample-loaded frozen carrier directly into liquid nitrogen. But because the biological sample is in direct contact with liquid nitrogen, the sterile environment for storing the biological sample can not be provided, the cross contamination risk exists, and the direct toxic effect of the liquid nitrogen on cells is not predictable (Covo A, Domingo J, Perez S, et al. vision: an effective new approach to cell cloning and preservation in cancer tissues [ J ]. Clin Transl Oncol,2008,10(5): 268-273; closed straw vitrification freezing method is used for the observation of the freezing effect of the early embryo of human, Shandong medicine 2010, 50 th volume at the bottom of 2010; 15 th volume), more recent studies have shown that low molecular weight compounds In liquid nitrogen are toxic to biological samples (Y Panagitids, P Vanderzwelmen, YPrapas, E kasapi, et al, al Open top closed vision of blast from an on-home program: a productive random synthesized student: reproduction biomedical one, In Press, unordered project, Mar 2013). Patent application (200710192245.9) discloses a sealed vitrification freezing device for preventing contamination of a biological specimen with exogenous pathogenic substances, but despite a closed system for isolating the biological specimen from a coolant, the operation of this type of freezing carrier is complicated by various steps such as thermoplastic sealing.

Moreover, when the freezing device is placed under the microscope device and observed together with the culture dish, the freezing device cannot be kept on the same horizontal plane with the culture dish due to the height of the freezing device, so that an operator needs to continuously adjust the focal length when switching and observing the freezing device sample and the culture dish sample.

Therefore, there is an urgent need in the art to develop a closed type freezing device for biological samples, which has high vitrification freezing efficiency, convenient operation, safety, no toxicity, and as small a volume as possible.

SUMMERY OF THE UTILITY MODEL

The purpose of this application is providing a biological sample vitrification carrier, convenient operation to the volume is further miniaturized, and the heat capacity reduces, and freezing efficiency is higher.

In one aspect of the present application, a biological specimen vitrification freezing carrier is disclosed, comprising: the device comprises a body and a sealing cap, wherein a frozen sample placing area is arranged at the near end of the body, a cavity which is mutually sealed is formed at the bottom of the frozen sample placing area and the bottom of the sealing cap, and a frozen sample placing platform is arranged at the bottom of the frozen sample placing area and is sunken towards the inner side of the body;

the body is provided with a coolant flow channel, and the sealing cap is provided with a flow guide hole corresponding to the coolant flow channel, so that when the biological sample is frozen, the coolant flowing in the channel is in contact with the outer surface of the frozen sample placing area and cools the biological sample in the frozen sample placing area.

In a preferred embodiment, the upper edge of the frozen sample placement region has an annular outward projection that allows the frozen sample placement region to be secured to the top of the proximal end of the body and to mate with the body when the frozen sample placement region is inserted into the body.

In a preferred embodiment, the body further comprises an engagement portion located at the distal end of the body; the engagement portion is adapted to sealingly engage the sealing cap when the sealing cap is mated with the body and to locate the body entirely within a cavity formed by the sealing cap.

In a preferred embodiment, the passage includes a distal opening at the engaging portion and a side opening provided in the side wall of the body, and the distal opening and the side opening communicate with each other.

In a preferred embodiment, the inner side wall of the distal end further has an auxiliary tool, and the auxiliary tool is used for matching and fixing the body and an external display instrument component.

In a preferred embodiment, the biological sample platform has a diameter of 1-15mm, and/or

The depth of the depression of the frozen sample placing area is 0.5-5 mm.

In a preferred embodiment, the carrier has a freezing rate of at least 30000 ℃ per minute after placement of the biological sample.

In a preferred embodiment, the material of the body is selected from the group consisting of: titanium alloy, aluminum magnesium alloy, stainless steel; and/or

The material of the sealing cap is selected from high polymer materials.

In a preferable embodiment, the top of the sealing cap is provided with a holding part, the holding part is arranged on the same side of the flow guide hole of the sealing cap, and the height of the holding part is 4-10 mm.

In a preferred embodiment, the sealing cap further comprises a sample information marking area, wherein the sample information marking area at least comprises the top and one side wall of the sealing cap and is suitable for being pasted with a label with the size of a multiplied by b, wherein a is more than or equal to 6mm and less than or equal to 28mm, and b is more than or equal to 6mm and less than or equal to 10 mm.

In another aspect of the present application, a microscopic observation lining plate is disclosed, comprising a culture dish placing area and a frozen biological sample carrier placing area, wherein

The culture dish placing area is provided with one or more culture dish placing positions;

the frozen biological sample carrier placing area is provided with one or more carrier placing positions;

the height of the place where the culture dish is placed and the height of the place where the carrier is placed form the same focusing distance with respect to the microscope.

In a preferred embodiment, the dish placement station has a concave slot adapted for dish placement.

In a preferred embodiment, the culture dish placement position is hollow throughout.

In a preferred embodiment, the carrier placement site is a pillar comprising an upper portion and a lower portion, the pillar fitting into an opening of the vitrified frozen carrier body.

In a preferred embodiment, the upper part of the upright post is a cylinder, and the lower part of the upright post is a cubic cylinder, and the upright post is matched with an auxiliary tool of the vitrification freezing carrier body.

In a preferred embodiment, the culture dish placement area and the frozen biological sample carrier placement area are raised to different thicknesses, wherein

The thickness range of the culture dish placing area is 1-3 mm;

the thickness of the frozen biological sample carrier placing area is smaller than that of the culture dish placing area, and the thickness range is 0.08-0.20 mm.

In a preferred embodiment, the culture dish placing position and the carrier placing position are configured such that when the culture dish loaded with embryos and cells is placed on the concave groove position while the carrier is fitted on the upright, the tolerance of the culture dish and the frozen sample placing platform of the carrier in the vertical direction is less than 0.05 mm.

In a preferred example, the number of the culture dish placing positions and the number of the carrier placing positions are in a range of 1 to 12, respectively.

In a preferred embodiment, the diameter of the concave slot is in the range of 6cm to 12 cm.

In a preferred embodiment, the material of the microscopic observation lining plate is selected from high light-transmitting materials. The application has at least the following technical effects:

1. the biological sample is stored in a sealed way, and the biological sample is safe and non-toxic: the carrier is used for isolating and hermetically storing the biological sample and the freezing liquid, is safe and pollution-free, and reduces the potential toxicity risk of the freezing liquid to the biological sample.

2. The freezing speed is fast, and the high-efficiency vitrification freezing is achieved: the carrier can reach the freezing rate of 30000-100000 ℃/min, thereby playing the role of rapid vitrification freezing and avoiding the influence of ice crystal damage in the process of freezing the biological sample.

3. The operation is convenient: the carrier adopts sealing modes such as thread fastening or sleeving, buckling fastening and the like, and when in operation, the biological sample can be placed on the placing area platform and then the sealing cap is screwed, and then the biological sample can be placed in liquid nitrogen for storage, so that the complex procedure of the traditional thermoplastic sealing is replaced.

4. Safe and effective: due to the special structure and simple operation of the device, the sample is taken out after the temperature is raised and is convenient as the sample is loaded, and the biological sample is not easy to lose after the temperature is restored.

5. The sealing structure is further optimized: the utility model realizes complete sealing by depending on the frozen sample placing area and the sealing cap, and does not need to be further provided with sealing components such as a sealing ring and the like.

6. The volume is tiny: the body needs to be capable of being installed and matched with an external instrument or a microscopic instrument part, an auxiliary tool (namely a square hole) of the body of the carrier is arranged on the inner side wall of the far end of the body, the thickness of the body is further reduced by at least 5mm, the height of the body is reduced, the volume of the body is smaller, and compared with a previous generation product with a wrench tool arranged outside, the volume and the height of the body are respectively reduced by at least 40%, so that the heat capacity of the body is further reduced, and the freezing rate is improved.

7. Sample information marking area enlargement: the sample information marking area is positioned on the sealing cap, is staggered with the positions of the diversion hole and the holding part, comprises the top and at least one side wall of the sealing cap, can also comprise the top and two side walls, is further extended and can be used for sticking various labels from squares (such as 6mmX6mm two-dimensional codes) to long strips (28mmX6 mm).

8. The microscopic observation lining plate used with the carrier is provided, so that a culture dish sample needing to be observed is lifted to the height same as that of a carrier sample placing area, the same focal length is easy to use for observation, the focal length of the microscope does not need to be readjusted for observation in different areas, and time and labor are saved.

Drawings

FIG. 1 is a right side cross-sectional view of a carrier according to the present application;

FIG. 2 is a front cross-sectional view of a carrier according to the present application;

fig. 3 is a top view of a carrier according to the present application;

fig. 4 is a bottom view of a carrier according to the present application;

fig. 5 is a perspective isometric view of a carrier according to the present application;

FIG. 6 is a top view of a microscopic viewing liner according to one embodiment of the present application;

FIG. 7 is a cross-sectional view of a micro-scope liner according to one embodiment of the present application;

FIG. 8 is a perspective isometric view of a microscopic viewing liner according to one embodiment of the present application.

Description of reference numerals:

1-body

11-proximal end

110-frozen sample Placement area

111-frozen sample placement platform

112-convex part

12-distal end

121-engaging part

122-auxiliary tool

14-side hole

15-passage for frozen liquid

2-sealing cap

201-diversion hole

21-grip part

22-sample information marker region

81-Petri dish Placement area

811-Petri dish Placement

8110 concave slot

82-frozen biological sample carrier placement area

821-column

Detailed Description

The present inventors have conducted extensive and intensive studies to provide a carrier for hermetically cryopreservation of a biological sample. Tests prove that the designed coolant flow channel, the coolant flow guide hole and the biological sample placing platform of the carrier can achieve efficient vitrification freezing effect while hermetically isolating the biological sample from freezing liquid, and the embryo is high in survival rate after being reheated and simple to operate. On the basis of this, the present invention has been completed.

Frozen sample placement platform and placement area

The present invention is a carrier assembly comprising two components: the body and the sealing cap define one end of the body that contacts the sealing cap when assembled as a "proximal end" and the other end as a "distal end". The near end of the body is provided with a frozen sample placing area which is bowl-shaped, the upper edge of the frozen sample placing area is provided with an annular outward bulge, 2-4 bulges are arranged around the outer diameter edge of the platform and contact with the near-end channel opening of the body, and the bulge ensures that the frozen sample placing area can be fixed at the top of the near end of the body when being embedded into the body and is tightly matched with the body; the placing area is sunken towards the inner side of the body to form a cavity, the depth of the cavity is 2mm-10mm, and the bottom of the cavity is the frozen sample placing platform. The frozen sample placement area is removable and may be secured to the top surface of the proximal end of the body by any of a variety of conventional fastening means. The frozen sample placing area and the inner bottom plane of the sealing cap form a sealed space together, sealing can be achieved without sealing parts (such as sealing rings), the frozen sample placing area is guaranteed to effectively isolate the biological sample from being in contact with a coolant, and therefore cross contamination and toxicity of toxic substances such as unknown pathogens in the coolant to the biological sample are prevented.

The material of the frozen sample placing area is a medical high polymer material which is nontoxic and can resist the changes of shock cooling and shock heating temperatures. Typically, materials that can be used in the frozen sample placement platform of the present invention include PE, PET, PP.

The material of the body is selected from titanium alloy, aluminum magnesium alloy and stainless steel. The sealing cap is made of medical polymer material.

The diameter of the frozen sample placing platform is 1-15mm, preferably 3-12mm, and more preferably 4-10 mm; most preferably 6-8 mm; and/or the thickness of the carrier platform is 0.06-0.0.20mm, preferably 0.07-0.12mm, more preferably 0.08-0.10 mm; the height of the frozen sample placement area is 0.5-5mm, preferably 0.8-2 mm.

In addition, the dimensions of the biological specimen vitrification freezing medium of the present invention may vary depending on the particular size of the biological specimen to be frozen and the operation of removing excess cryoprotectant.

Refrigerating fluid flow channel

The coolant flow channels useful in the present invention include the following:

(a) a distal opening at the distal end of the body;

(b) a side opening at the proximal end of the body;

(c) and the flow guide hole is positioned on the side wall of the sealing cap and corresponds to the opening position of the near end side of the body.

The far-end opening and the side opening are communicated with each other through the center of the body and correspond to the flow guide holes in the side wall of the sealing cap. The side opening is positioned at the near end of the body, and preferably, the side opening is tightly attached to the frozen sample placing platform.

Typically, the number of side openings and flow directing holes is at least two, and more preferably four or more.

In the utility model, after the sealing cap and the body are mutually closed and sealed, the sealing cap is put into the freezing liquid, and the freezing liquid can be circularly communicated through the freezing liquid flowing channel from the far end opening to the side opening to the flow guide hole, so that the leidenfrost effect can be reduced by the cooling liquid flowing channel, and the tissue sample positioned on the freezing placement platform can be rapidly cooled.

In the utility model, the side opening of the body is an elliptical transverse hole with the diameter of 1-5mm or 3 multiplied by 5mm, and the guide hole of the sealing cap is an elliptical transverse hole with the diameter of 2-7mm or 3 multiplied by 6 mm.

The three-dimensional shape of the distal end opening or the body side opening or the flow guide hole which can be used in the present invention is not particularly limited, and may be any shape which is advantageous for the circulation of the refrigerant and is matched with each other, and generally, the three-dimensional shape of each opening or flow guide hole in the present invention is a stepped shape or a truncated cylindrical shape.

Meshing part and auxiliary tool

The body of the utility model is provided with a meshing part at the far end, the meshing part is an external thread, and the lower part of the sealing cap is provided with an internal thread matched with the external thread. In the present invention, the external thread of the engaging portion may have a nominal diameter of M4 to M12, preferably a T-shaped M8-M10 external thread. When the sealing cap is mated with the body, the engaging portion is used for sealing engagement with the sealing cap, and the body is completely positioned in the cavity formed by the sealing cap, namely the sealing cap can completely cover the body.

The inside wall of the distal end of body still has auxiliary fixtures. Specifically, the auxiliary tool is a regular polygonal hole groove which forms a part of the refrigerating fluid flow channel. Due to the through hole, light can pass through the auxiliary tool, and the auxiliary tool can be used with an external microscopic instrument or a matched component. For example, when a biological sample is contained in the carrier, the carrier is placed under a microscope for observation, and light can pass through the auxiliary tool for observation of the sample. In some embodiments, the auxiliary tool is matched with the shape of a microscopic observation lining plate, and the auxiliary tool is buckled on the upright post of the microscopic observation lining plate and can be fixed; then the sealing cap is screwed on the body, when the sealing cap is screwed on, the auxiliary tool fixes the position of the sealing cap without sliding or deviating, and the sealing cap can be sealed on the body to form a carrier. When the sealing cap is unscrewed, the auxiliary tool also fixes the position of the sealing cap, does not slide or deviate, and opens the sealing cap, so that the sealing is released. Preferably, the hole groove is a square hole, and the side of the cross section of the square hole is 2mm-8mm, preferably 6 mm.

Micro-observation lining board

In one embodiment, the device further comprises a microscopic observation lining plate. The lining plate comprises a culture dish placing area and a frozen biological sample carrier placing area. The thickness of the culture dish placing area is 1-3mm, and one or more culture dish placing positions are arranged. The culture dish placing position is provided with a concave groove position, the concave groove position is suitable for placing a culture dish with the diameter of 6-12cm and is hollow and penetrated, and the concave groove position is used for enabling the light of a microscope light source below to pass through. The thickness of the frozen biological sample carrier placing area is smaller than that of the culture dish placing area, and is generally 0.08mm-0.20 mm; one or more upright posts (i.e. carrier placement positions) are arranged on the support. The stand includes upper portion and lower part, and preferably upper portion is the cylinder, with the runner adaptation of the last indent of carrier body, the lower part is cubic cylinder, and with the supplementary frock adaptation of the inside circulation passageway of body and carry the function that the sealing cap seals or the deblocking. The side length of the square hole section of the cubic column is 2mm-8mm, preferably 6 mm. The height of the upright post is 5-8 mm. When the culture dish including the embryonic cells is placed on the concave groove position and the carrier is adapted to the upright column, the tolerance of the culture dish and the frozen sample placing platform of the carrier in the vertical direction is less than 0.05mm, namely, the two observation planes are positioned on the same plane. The culture dish sample and the frozen biological sample placed on the microscopic observation lining plate can be used for the same focal length observation of the microscope.

The number range of the culture dish placing positions and the number range of the carrier placing positions are respectively 1-12. Preferably, the number of the culture dish placing positions and the number of the carrier placing positions are respectively 4-6.

The microscopic viewing mount uses a highly transparent material, such as PS, PC, PMMA, to minimize loss of light from the source of the microscope.

Sealing cap and sample information marking area

The sealing cap of the present invention has a grip portion. The holding part is a holding rod protruding from the top of the sealing cap, is integrally formed on the sealing cap, and has a height of 4-10mm, preferably 5-8 mm; preferably on the opposite side of the deflector hole of the sealing cap, i.e. on the opposite side (non-adjacent side). The other two adjacent sides of the sealing cap are free. In a preferred embodiment, the sealing cap further comprises a sample information marking zone, which can cover a vacant part of the sealing cap, can be elongated, thereby at least comprising a top part and a side wall of the sealing cap, and is adapted to adhere a label with a size of 6mm × 6mm to 6mm × 28 mm.

Method for rapidly freezing biological sample

The method for rapidly freezing the biological sample comprises the following steps:

a) providing a biological sample vitrification freezing carrier, wherein the carrier comprises a body and a sealing cap, and the biological sample is placed on a frozen sample placing platform of the biological sample vitrification freezing carrier, and redundant freezing protection liquid is removed under microscopic observation, so that a carrier body containing the biological sample is formed;

b) engaging and screwing the biological sample-containing carrier body of step a) with the sealing cap, thereby forming a sealed carrier containing a biological sample;

c) marking the sample information on the sealed carrier containing the biological sample in the step b) in a marking area or sticking a label printed with the sample information to the marking area of the sealing cap;

d) and c) placing the sealed carrier marked with the biological sample information in the step c) into a cooling agent for preservation.

Generally, the method for hermetically vitrification freezing the biological sample further comprises labeling the sealed carrier containing the biological sample; and/or marking the frozen carrier, placing the specimen and sealing to obtain the marked sealed carrier.

The vitrification freezing carrier and the method for quickly freezing the biological sample can achieve the cooling rate of 30000-.

In addition, when the carrier is applied to experiments or diagnosis and treatment, the carrier needs to be used after being sterilized and disinfected, and is operated in a sterile environment.

Materials and appearance

The material used for preparing the vitrified frozen carrier for the biological sample is a high molecular material or metal which is nontoxic and can resist the temperature change of shock cooling and shock heating.

In another preferred embodiment, the polymer material includes PE (polyethylene), PP (polypropylene); PET (polyethylene terephthalate).

In another preferred example, the metal comprises stainless steel, titanium alloy and aluminum magnesium alloy.

In another preferred example, the polymer material or metal is a medical polymer material or metal.

In the utility model, the body and the sealing cap are mutually buckled and sealed in various movable sleeving ways such as mutual buckling or threaded rotary connection. Generally, the fastening or threaded rotary connection device can achieve the sealing effect through various conventional methods, such as changing the shape or angle of threads and the like, and ensure the sealing self-locking of the sealing device in the quenching and rapid heating process.

The shapes of the body and the sealing cap are not particularly limited, and can be any matched cylindrical shapes, such as a cylinder shape and a hexagonal cylinder shape, so that the body and the sealing cap are convenient for an operator to hold and stabilize the carrier when placing biological samples such as embryos, ova and the like. In addition, the body and the sealing cap of the utility model can adopt various colors to distinguish the carrier used for the cryopreservation identification of different biological samples, tissues, cells and the like.

The utility model has the beneficial effects that:

1. the biological sample is stored in a sealed way, and the biological sample is safe and non-toxic: the carrier and the method are used for isolating and hermetically storing the biological sample and the freezing liquid, are safe and pollution-free, and reduce the potential toxicity risk of the freezing liquid to the biological sample and the assumed cross contamination of pathogenic bacteria in liquid nitrogen.

2. The freezing speed is fast, and the high-efficiency vitrification freezing is achieved: the carrier and the method can achieve the freezing rate of 30000-. Thereby achieving rapid vitrification freezing and avoiding the biological sample from being damaged by ice crystals in the freezing process.

3. The operation is convenient: the carrier adopts sealing modes such as thread fastening or sleeving, buckling fastening and the like, and when in operation, the biological sample can be placed on the platform and then the sealing cap is screwed, and then the biological sample can be placed in liquid nitrogen for storage, so that the complex procedure of the traditional thermoplastic sealing is replaced.

4. Safe and effective: because the special structure and the operation of the device are simple and easy, the sample is taken out after being reheated and is convenient as the sample is loaded, and because the biological sample placing area is concave, the biological sample is not easy to lose in the whole temperature reduction and heating process.

Claims

1. A microscopic observation lining plate is characterized by comprising a culture dish placing area and a frozen biological sample carrier placing area, wherein

2. The microscopy observation liner according to claim 1, wherein the culture dish placement site has a concave slot adapted for culture dish placement.

3. The microscopy observation liner according to claim 1, wherein the culture dish placement site is hollow throughout.

4. The microscopy observation liner according to claim 1, wherein the carrier placement site is a column comprising an upper portion and a lower portion, the column fitting into the opening of the vitrified frozen carrier body.

5. The microscopy observation liner plate according to claim 4, wherein the upper part of the upright post is a cylinder, and the lower part of the upright post is a cubic cylinder, and the column is matched with an auxiliary tool of the vitrification freezing carrier body.

6. The microscopic observation liner of claim 1, wherein the culture dish placement region and the frozen biological sample carrier placement region have different raised thicknesses, and wherein

The thickness range of the culture dish placing area is 1-3 mm;

7. The microscopy observation liner according to claim 1, wherein the culture dish placement site and the carrier placement site are configured such that when the embryo-and cell-loaded culture dish is placed on the recessed slot while the carrier is fitted on the upright, the tolerance of the culture dish to the frozen sample placement platform of the carrier in the vertical direction is less than 0.05 mm.

8. The microscopy observation liner plate according to claim 1, wherein the number of the culture dish placing positions and the number of the carrier placing positions are respectively in the range of 1-12.

9. The microscopy observation liner according to claim 2, wherein the diameter of the concave groove is in the range of 6cm-12 cm.

10. The microscopy scope liner according to claim 1, wherein the material of the microscopy liner is selected from the group consisting of highly light transmissive materials.