CN203484166U - Microfluidic chip for environmental monitoring - Google Patents
Microfluidic chip for environmental monitoring Download PDFInfo
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- CN203484166U CN203484166U CN201320490996.XU CN201320490996U CN203484166U CN 203484166 U CN203484166 U CN 203484166U CN 201320490996 U CN201320490996 U CN 201320490996U CN 203484166 U CN203484166 U CN 203484166U
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Abstract
The utility model discloses a microfluidic chip for environmental monitoring. The microfluidic chip comprises a substrate, wherein a processor and two separately arranged sensing electrodes are arranged on the substrate, a testing electrode is arranged between the two sensing electrodes, and the testing electrode and the two sensing electrodes are respectively connected with the processor for transmitting detecting signals; and a water absorption film is arranged above the two sensing electrodes and the testing electrode. The microfluidic chip disclosed by the utility model is used for solving such problems of traditional instrument and equipment used for monitoring trace heavy metal elements in the environment as high manufacturing cost, incapability of detecting a complicated environmental sample and the like, and the microfluidic chip has the advantages of being simple in operation, accurate in detection result, reliable and completely automatic and the like, so that the microfluidic chip can be widely applied to quick qualitative and quantitative analysis of trace heavy metal elements in the environment to improve the real-time performance and rapidity of environmental monitoring, save the cost in the environmental monitoring process and improve the monitoring efficiency, and the microfluidic chip has a wide application space in such fields as onsite quick environmental monitoring, etc.
Description
Technical field
The utility model belongs to enviromental monitoring equipment technical field, specifically, relates to a kind of micro-fluidic chip technology for detection of trace heavy metal content in fluid sample.
Background technology
Along with China's expanding economy, problem of environmental pollution highlights day by day, has caused the country and people's extensive concern, and wherein, heavy metal is one of harmful pollution factor of tool potential threat.Because heavy metal pollution can not be decomposed by environmental microorganism, and microbial body has the ability of enriching heavy metal, so heavy metal also can produce bad consequence in the situation that of trace extremely, and the various ecosystems and the mankind's life security in serious threat.Given this, the quantitative analysis of trace heavy metal just becomes extremely important in the detection of the aspects such as ecology, food, environment.
At present, for detection of the means of trace heavy metals, be to rely on the large-scale instruments such as atomic absorption spectrophotometer, mass spectrum, neutron activation analysis to test, its shortcoming mainly: need large-scale, accurate instrument and equipment, longer analysis time, complicated sample pre-treatments and analytic process etc.A topmost problem is that these methods can only be carried out in laboratory, can not use at Test Field, all needing to deliver to laboratory after spot sampling analyzes, therefore, the cost of traditional detection method is high, efficiency is low, and owing to not detecting in time sample, the variation midway occurring and the pollution in sample transmitting procedure all can have influence on the objectivity of testing result.So, develop the simple and direct quick of heavy metal element, high sensitivity, the detection method of high selectivity and instrument, real-time, online, the original position that realize heavy metal detection have very important scientific meaning and using value.
Summary of the invention
The utility model for solve the existing instrument and equipment manufacturing cost for detection of trace heavy metals high, cannot carry out to sample the problem of direct-detection, a kind of environmental monitoring micro-fluidic chip simple in structure, with low cost has been proposed, to realize the fast qualitative of trace heavy metals in environmental sample, quantitative analysis, improve detection efficiency.
In order to solve the problems of the technologies described above, the utility model is achieved by the following technical solutions:
A kind of environmental monitoring micro-fluidic chip, comprise substrate, on substrate, be provided with processor and two induction electrodes of separately laying, between two induction electrodes, be provided with test electrode, described test electrode is connected respectively described processor with two induction electrodes, transmission detection signal; Above described two induction electrodes and test electrode, be provided with water-absorbing film.
Described water-absorbing film is disposable diaphragm, in order to be convenient for changing described water-absorbing film, preferably described water-absorbing film is directly overlayed to the top of described two induction electrodes and test electrode.
Preferably, described water-absorbing film preferably adopts the materials such as polyvinyl alcohol foam, polyacrylamide film or cotton fiber filter paper to be made.
In order to be convenient for changing test electrode, preferably described test electrode is arranged on to one independently on battery lead plate, on described substrate, be provided with the slot for battery lead plate described in plug-in mounting, in described slot, be provided with the metal contact piece being connected with described processor, described metal contact piece is after battery lead plate is inserted in slot, just contact with the metal contact piece of connecting test electrode on battery lead plate, and then realize the connecting communication of processor on test electrode and substrate.
Further, be provided with three test electrodes on described battery lead plate, one of them is reference electrode, and two other is working electrode, three test electrodes respectively with battery lead plate on three metal contact pieces laying connect one to one.
For fear of environment is caused to secondary pollution, described reference electrode preferably adopts silver/silver chloride electrode, and surface attachment has the silver electrode of silver chlorate; Two described working electrodes are selected respectively nanometer bismuth film electrode and graphite electrode, by adopting nanometer bismuth film electrode to replace traditional mercury electrode as polarography determination electrode, thereby can reduce environmental pollution.
Further, in described environmental monitoring with being also provided with cover plate in micro-fluidic chip, on cover plate, offer sample inlet and watch window, described sample inlet and watch window are just relative one by one with the position of described two induction electrodes after cover plate fastens with substrate.
In order to improve the sealing between cover plate and substrate, between described cover plate and substrate, be also provided with sealing strip.
In order to realize quick-detachment and the assembling of micro-fluidic chip, at the relative position place of described cover plate and substrate, also correspondence is provided with magnetic material and paramagnetic material respectively, utilizes the effect of magnetic material absorption, realizes automatic pick and the rapid-assembling of cover plate and substrate.
Preferably, described cover plate is preferably adopted made of plastic, to reduce the holistic cost of micro-fluidic chip.
In order to detect transfer of data to peripheral watch-dog, to realize analysis, the demonstration of testing result, on described substrate, be also provided with the peripheral interface for external data line, such as USB interface etc., connect described processor, the testing result of the detection data that collect in processor and generation is sent to outside watch-dog, to facilitate monitoring personnel observation.
Compared with prior art, advantage of the present utility model and good effect are: environmental monitoring micro-fluidic chip of the present utility model, solved tradition high for the instrument and equipment manufacturing cost of monitoring of environmental trace heavy metals, cannot carry out to complex environment sample the problems such as direct-detection, have simple to operate, testing result is accurate, the advantages such as reliable and full automation, can be widely used in fast qualitative and the quantitative analysis of Environmental Trace heavy metal element, to improve real-time and the rapidity of environmental monitoring, saved the cost in environmental monitoring process, improved monitoring efficiency, in fields such as the on-the-spot fast monitored of environment, there is wide application space.
Read by reference to the accompanying drawings after the detailed description of the utility model embodiment, other features of the present utility model and advantage will become clearer.
Accompanying drawing explanation
Fig. 1 is the decomposition texture schematic diagram of a kind of embodiment of micro-fluidic chip for the environmental monitoring that proposes of the utility model;
Fig. 2 is the structural representation that is laid in a kind of embodiment of the magnetic attracting device on Fig. 1 cover plate and substrate;
Fig. 3 is the structural representation after Fig. 1 cover plate and substrate adhesive;
Fig. 4 is the structure perspective view after the assembling of micro-fluidic chip shown in Fig. 1.
The specific embodiment
Below in conjunction with accompanying drawing, the specific embodiment of the present utility model is described in more detail.
The concept of micro-full analytical system proposed in early 1990s, was to integrate sample introduction, sample treatment, separated Highgrade integration, microminiaturization and the intelligentized analytical system detecting, and was called again chip lab.Groundwork principle is: the platform that constructs the functions such as preparation, mixing, extraction, reaction and detection that can complete sample in traditional experiment chamber on microchip, foundation is with the chemical analysis system of the multi-field intersections such as computer science, biology, realized from sample pretreating to the integral body detecting microminiaturized, integrated and portability, be technological core portable, automated analysis instrument.
The range of application of microflow control technique has comprised from printer ink sprinkler head, chemical reaction process, fuel cell until closely-related for fields such as POCT and the portable instrument of imitation biochemistry war, the automatic chemical feeding device of implanted and high-throughout drug screenings with bioscience.
Micro-fluidic chip appear at the limitation that has made up to a great extent traditional chemical laboratory, such as conventional analysis experiment, need the participation of larger lab space, multiple instrument, the operating personnel of specialty etc.And under the micro-fluidic chip operating condition complicated not needing specialty, can carry out real-time field monitoring to many fields, this lays a good foundation for the application of chemical analysis test technology.
Micro-fluidic chip adopts monocrystalline silicon as the material of processing microchip the earliest, but this material exists shortcomings such as expensive, opaque, frangible and poor insulativity.Afterwards, quartzy and glass relied on its good optical property, insulating properties and heat conductivility, gradually became one of preferred material of processing microchip.But quartzy and glass still exists the problems such as processing cost is high, easy cracked, therefore neither process the ideal material of micro-fluidic chip.Development along with new material, new technology, macromolecule polymer material because cost is low, of a great variety, good insulating and be easy to machine-shaping and the feature such as mass production, become the leading material of microchip processing, such as polymethyl methacrylate (claim not only acrylic, lucite) and dimethyl silicone polymer (but also claiming silicon rubber) etc.But, domesticly still lacking ripe process technology, processing and use cost are still higher, so only rest at present conceptual phase, are difficult to apply in actual testing process.
In view of above designing technique, mainly there is following problem in the micro-fluidic chip of present stage:
The one,, the more difficult microminiaturization of detecting instrument and portability that current micro-fluidic chip need to be used while working, trace it to its cause, and is mainly reflected in following two aspects:
1, for realizing abundant contact, the reaction or separated of sample and reagent, must have the effect of external force, this external force is generally the power that electric field force, normal pressure or negative pressure produce.The positive and negative pressure that the electric field force that the normal employing of people is produced by high voltage source or pump produce is as drive source, and wherein, dynamical system is all additionaling power source substantially, and for example electrophoretic techniques, needs the support of high voltage source; Micropump technology easily produces and pollutes sample, and fault rate is relatively high.And, in use procedure, needing power supply, carrier fluid, carrier gas and cleaner working environment, this has limited the Site Detection ability of micro-fluidic chip to sample under complex environment to a great extent.
In order to address this problem, the present embodiment utilizes capillary siphoning principle, adopt macromolecular material to manufacture water-absorbing film, without auxiliary power devices such as configuration pump, valves, can realize flowing of sample, from having facilitated in essence integrated to multiple functional hand-held analyzer device, contribute to the realization of field quick detection.
2, the most frequently used detection means is via Laser-induced Fluorescence Method at present, in addition, also has the methods such as electrochemistry, mass spectrum, ultraviolet, chemiluminescence and sensor detection.These detection methods all need to configure in addition the equipment such as Electrochemical Detection station, large-scale spectra collection instrument, cause thus the increase of whole detection system volume.
In order to address this problem, the present embodiment adopts data processing integrated technology, data processing section is integrated in micro-fluidic chip, and by using polarography determination technology, has significantly dwindled the overall dimensions of detection system, provides cost savings.
The 2nd,, current micro-fluidic chip longevity of service, sample all need to expend the longer time by micro-fluid chip surveyed area, and is difficult to guarantee that sample all passes through.For most of Electrochemical Detection means, authentic sample always needed it to carry out pretreatment before detecting, because being easy to be subject to the impact of physical factor (as bubble or pollutant etc.), the signal of electrode detection produces interference, and therefore cannot be by allowing the method that sample all flows through surveyed area carry out concentration determination.
The present embodiment adopts capillary siphoning principle, utilizes speciality polymer fibrous material to make water-absorbing film, and as the power source of sample, speed is quick.
The 3rd,, the process technology of micro-fluidic chip is still more complicated, and to having relatively high expectations of process equipment and rapidoprint, for example chemical etching technical matters is complicated, time-consuming, and to the having relatively high expectations of manufacturing environment, thereby cause manufacturing cost too high.Not outstanding in the research application of this problem in laboratory, but in the practical application of technical grade, can't reach the level of large-scale mass production, thereby can not meet to low-cost, process the wilderness demand of easy micro-fluidic chip technology, be not easy to produce in enormous quantities.
In order to address this problem, the present embodiment adopts the plastic moulds technology of technology maturation, select cheaply plastics (such as tetrafluoroethene, polyvinyl chloride etc.) to carry out injection moulding and produce micro-fluidic chip, thus can be in enormous quantities, low-cost production, easily realize industrialization.
The 4th,, existing polarography determination technology, generally adopts dropping-mercury electrode, thereby easily environment is caused to secondary pollution.
The present embodiment adopts bismuth film to replace mercury to make the test electrode in micro-fluidic chip, by using the content of beary metal in environment-friendly type polarography determination sample, thereby has solved problems.
The concrete structure design of micro-fluidic chip the present embodiment being proposed below in conjunction with Fig. 1-Fig. 4 is at length set forth.
Shown in Figure 1, in the micro-fluidic chip of the present embodiment, be mainly designed with substrate 1, described substrate 1 can directly be selected printed substrate, lays such as CPU or MCU etc. of processor 5(on it), induction electrode and test electrode.Wherein, induction electrode is provided with two, is respectively the induction electrode 2 that whether injects for detection of sample solution and for detection of the whether saturated induction electrode 3 of absorbent material.Two induction electrodes are opened and are laid on substrate 1 for 2,3 minutes, lay described test electrode therebetween, and the water-absorbing film 4 that setting is made by hydrophilic fiber material above described two induction electrodes 2,3 and test electrode, utilize water-absorbing film 4 as power source, the sample solution that is injected into induction electrode 2 is drained to another one induction electrode 3.At sample solution, from induction electrode 2, be drained to the process of induction electrode 3, sample solution inevitably can touch test electrode, thereby starts the testing process to trace heavy metals in sample solution.Described two induction electrodes 2,3 are connected to described processor 5 by the PCB cabling on substrate 1 respectively with test electrode, to realize automatically carrying out of testing process.
In the present embodiment, described water-absorbing film 4 preferably adopts the hydrophilic materials such as polyvinyl alcohol foam, polyacrylamide film or cotton fiber filter paper to be made, and it is that 1cm left and right, length are that 7cm left and right, thickness are in the scope of 0.2mm left and right that its size can be controlled at width.Described water-absorbing film 4 is as dynamical system and the filtration system of sample solution, its operation principle is: because the inside at water-absorbing film 4 exists a lot of capillaries, therefore can be without extrinsic motive, sample solution to be detected can flow on its surface by these siphonages capillaceous, reaches the object of sample solution automatic flowing.On the other hand, due to the surface of water-absorbing film 4 have countless apertures for liquid particle pass through, the solids that volume is larger can not pass through, therefore, can carry out pre-treatment to complicated sample solution, stop passing through of solid particle, bubble, not only avoided the pollution to test electrode, and can carry out enrichment and detection to the heavy metal ion in actual sample solution.
Due to after the testing process of sample solution finishes at every turn, all need to change water-absorbing film 4, to avoid the sample solution of last time to remain in the detection to next sample solution in water-absorbing film 4, impact, the present embodiment is designed to disposable diaphragm by described water-absorbing film 4, directly overlay two described induction electrodes 2, 3 and the top of test electrode, be water-absorbing film 4 and two induction electrodes 2, 3 and test electrode between without fixed connection structure, before starting, detection only water-absorbing film 4 directly need be put to two induction electrodes 2, 3 and test electrode directly over, to be convenient for changing water-absorbing film 4.
For test electrode, the present embodiment selects nanometer bismuth film electrode to replace traditional dropping-mercury electrode, as working electrode, for the test of sample solution, to avoid that environment is caused to secondary pollution.Because bismuth film can produce and consume in test process, must change in use for some time, therefore,, in order not destroy under the prerequisite of micro-fluidic chip, facilitate monitoring personnel to change test electrode, the present embodiment is preferably arranged on described test electrode separately one independently on substrate 10, for distinguish clear for the purpose of, hereinafter referred to as described substrate 10, be battery lead plate, shown in Figure 1, and the mode of adopt pegging graft, realize movable connection the between battery lead plate 10 and substrate 1.Specifically, can slot 11 be set on described substrate 1, metal contact piece 12 is set in slot 11.Test electrode is arranged on battery lead plate 10, by the PCB cabling on battery lead plate 10, connects and be laid in the metal contact piece 13 on battery lead plate 10.Battery lead plate 10 is inserted in the slot 11 on substrate 1, make metal contact piece 13 on battery lead plate 10 just with the corresponding contact of metal contact piece 12 of laying in slot 11, and then realize being electrically connected between each test electrode on battery lead plate 10 and processor 5 on substrate 1, meet the transmission requirement of detection signal.
The present embodiment adopts Stripping Voltammetry detection method to detect the trace heavy metals in sample solution.Based on this detection mode, the present embodiment is provided with three test electrodes on battery lead plate 10, as shown in Figure 1, comprises a reference electrode 14 and two working electrodes 15,16.Wherein, described reference electrode 14 is silver/silver chloride electrode, and surface attachment has the silver electrode of silver chlorate, can adopt silver electrode is immersed in hydrochloric acid, the mode that makes silver electrode surface form one deck silver chlorate film is made, and preferred cloth is located at the left side of battery lead plate 10; One of them working electrode is the preferred nanometer bismuth film electrode of bismuth film electrode 15(), be laid in the middle part of battery lead plate 10, another one working electrode 16 is graphite electrode 16, is laid in the right side of battery lead plate 10.Three electrode 14-16 connect one to one by three metal contact pieces 13 laying on three PCB cablings on battery lead plate 10 and battery lead plate 10 respectively; In the slot 11 of substrate 1, lay too three metal contact pieces 12, when battery lead plate 10 inserts, for three metal contact pieces 13 corresponding contact conducting one by one with on battery lead plate 10, transmit three road detection signals, via the PCB cabling on substrate 1, be sent to respectively three pins of processor 5.Processor 5 is according to the scope range of the fluctuation of voltage of the detection signal receiving, utilize its inner software program to complete chemical analysis and the detection of heavy metal element in sample solution, and generate testing result and transfer to the peripheral interface 17 being laid on substrate 1, shown in Fig. 4, and then by transferring to outside watch-dog with the mutually external data wire of described peripheral interface 17, such as computer etc., realize the output display of testing result, to offer tester's observation.
In the present embodiment, described peripheral interface 17 can be selected the general general-purpose interfaces such as USB at present, by the external computer of usb data line, shown in Figure 4, to complete the output display that micro-fluidic chip is detected to data.
Described battery lead plate 10 can adopt the mode of serigraphy to carry out large batch of production, and plated film and the manufacture method of electrode are a lot, as conventional methods such as flying print, vapour deposition, magnetron sputterings, at this, no longer describe in detail.
In the micro-fluidic chip of the present embodiment, be also provided with cover plate 7, shown in Figure 1, for the consideration that reduces micro-fluidic chip cost, preferably adopt the plastic materials such as polytetrafluoroethylene (PTFE), polyvinyl chloride, polyethers ether copper, make full use of plastic moulds technology and manufacture described cover plate 7, to realize the low cost fabrication in enormous quantities of micro-fluidic chip.
On described cover plate 7, offer two through holes 8,9, a through hole 8 is for injecting sample solution, be referred to as sample inlet, after cover plate 7 and substrate 1 fasten, just corresponding with the position that is laid in the induction electrode whether injecting for detection of sample solution 2 on substrate 1; Another through hole 9 is referred to as watch window, be used for observing sample solution and whether by water-absorbing film 4, drain into induction electrode 3 on substrate 1, it offers position with after fastening at cover plate 7 and substrate 1 on cover plate 7, described watch window 9 just with relative being as the criterion in position of induction electrode 3.
In order to improve the sealing of cover plate 7 and substrate 1, between described cover plate 7 and substrate 1, be also further provided with sealing strip 6, as shown in Figure 1, preferably adopt silica gel strip to be designed to C shape, opening part is towards laying the side of battery lead plate 10 with water-absorbing film 4, can directly silica gel strip 6 be sticked on to the bottom surface of cover plate 7, to guarantee combining closely of cover plate 7 and substrate 1.
In order to realize rapid-assembling and the dismounting of cover plate 7 and substrate 1, the present embodiment is also respectively at cover plate 7 and corresponding magnetic material 18 and the paramagnetic material 19 of arranging on substrate 1, shown in Figure 2, such as: at magnetic materials 18 such as the embedded magnet in the left and right sides of cover plate 7, magnet steel, at paramagnetic materials 19 such as the embedded steel discs of the left and right sides of substrate 1 correspondence position, utilize the suction-operated of magnetic material, realize the automatic pick of cover plate 7 and substrate 1, rapid-assembling.Micro-fluidic chip after assembling is shown in Figure 3, and water-absorbing film 4 can directly be clipped between cover plate 7 and substrate 1, and battery lead plate 10 can be inserted in slot 11 after chip assembling, to complete the integral body assembling of micro-fluidic chip.
Below in conjunction with Fig. 4, the method for testing of the present embodiment is at length set forth.
First, take off the cover plate 7 of micro-fluidic chip, pack battery lead plate 10 and disposable water-absorbing film 4 into; Then, then cover plate 7 is installed and gone back, again fasten (magnet attraction, Automatic-clamping) with substrate 1.Connection data line on peripheral interface 17, and by data wire, connect the watch-dogs such as computer.
The testing sample solution that splashes into 100 μ L left and right by sample inlet 8, testing sample solution is contact induction electrode 2 first, and induction electrode 2 produces detection signal because sensing splashing into of sample solution, feeds back to processor 5, starts to carry out timing.Meanwhile, the sample solution splashing into touches to be had after hydrophilic water-absorbing film 4, and the direction to induction electrode 3 under the siphonage in water-absorbing film 4 internal capillaries is flowed.Sample solution is in mobile process, and because of the filtration of hydrophilic fibers in water-absorbing film 4, the bulky grain thing in sample solution and bubble, by separated, have been realized the purified treatment to sample solution thus.
When sample solution arrives test electrode 14-16 by water-absorbing film 4, processor 5 starts to carry out the analysis of content of beary metal in sample solution, and transfers to acquisition workstation by data wire, such as watch-dogs such as computers.When sample solution arrives saturation induction electrode 3, processor 5 judges that water-absorbing film 4 is saturated, completes analysis and the data acquisition of sample solution, and points out monitoring personnel to change water-absorbing film 4, carries out the test job of next sample.
After ensuing repeatedly sample test work completes, the bismuth film on the nanometer bismuth film electrode 15 on battery lead plate 10 may run out of, and now needs to change battery lead plate 10, to guarantee that ensuing test job carries out smoothly.
The micro-fluidic chip of the present embodiment adopts hydrophilic water-absorbing film as dynamical system and filtration system, utilize capillary pipe structure that water-absorbing film inside is rich in as power source and the filtering bodies of sample, not only realized the direct injected of sample, and without sample is carried out to pre-treatment, the existing volumes of liquid-driving mode such as existing high-voltage power supply, pump class have been overcome thus large, the problems such as easily loss, pre-treatment time length.
Certainly; above-mentioned explanation is not to restriction of the present utility model; the utility model is also not limited in above-mentioned giving an example, and the variation that those skilled in the art make in essential scope of the present utility model, remodeling, interpolation or replacement, also should belong to protection domain of the present utility model.
Claims (10)
1. an environmental monitoring micro-fluidic chip, it is characterized in that: comprise substrate, on substrate, be provided with processor and two induction electrodes of separately laying, between two induction electrodes, be provided with test electrode, described test electrode is connected respectively described processor with two induction electrodes, transmission detection signal; Above described two induction electrodes and test electrode, be provided with water-absorbing film.
2. environmental monitoring micro-fluidic chip according to claim 1, is characterized in that: described water-absorbing film is disposable diaphragm, directly overlays the top of described two induction electrodes and test electrode.
3. environmental monitoring micro-fluidic chip according to claim 2, is characterized in that: described water-absorbing film is polyvinyl alcohol foam, polyacrylamide film or cotton fiber filter paper.
4. environmental monitoring micro-fluidic chip according to claim 1, it is characterized in that: described test electrode is arranged on one independently on battery lead plate, on described substrate, be provided with the slot for battery lead plate described in plug-in mounting, in described slot, be provided with the metal contact piece being connected with described processor, described metal contact piece, after battery lead plate is inserted in slot, just contacts with the metal contact piece of connecting test electrode on battery lead plate.
5. environmental monitoring micro-fluidic chip according to claim 4, it is characterized in that: on described battery lead plate, be provided with three test electrodes, one of them is reference electrode, two other is working electrode, and two working electrodes are respectively nanometer bismuth film electrode and graphite electrode, three test electrodes respectively with battery lead plate on three metal contact pieces laying connect one to one.
6. according to the environmental monitoring micro-fluidic chip described in any one in claim 1 to 5, it is characterized in that: in described environmental monitoring with being also provided with cover plate in micro-fluidic chip, on cover plate, offer sample inlet and watch window, described sample inlet and watch window are after cover plate and substrate fasten, just relative one by one with the position of described two induction electrodes.
7. environmental monitoring micro-fluidic chip according to claim 6, is characterized in that: between described cover plate and substrate, be provided with sealing strip.
8. environmental monitoring micro-fluidic chip according to claim 6, is characterized in that: at the relative position place of described cover plate and substrate, correspondence is provided with magnetic material and paramagnetic material respectively.
9. environmental monitoring micro-fluidic chip according to claim 6, is characterized in that: described cover plate is adopted made of plastic.
10. according to the environmental monitoring micro-fluidic chip described in any one in claim 1 to 5, it is characterized in that: on described substrate, be also provided with the peripheral interface for external data line, described peripheral interface connects described processor.
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| CN104267073A (en) * | 2014-08-11 | 2015-01-07 | 西安交通大学 | Method for detecting water pollutant biotoxicity by paper-based micro-fluidic chip anode current |
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