CN202351223U - Solid-phase extraction column used for measuring benzo[alpha]pyrene in cigarette smoke - Google Patents

Abstract

The utility model belongs to analytical chemistry facilities, in particular to a solid-phase extraction column used in the analysis of benzo[α]pyrene in cigarette smoke. The solid-phase extraction column includes a column cavity and a column cap. A sieve plate is arranged at the upper, middle and lower positions of the column cavity, which are divided into two packing chambers. One packing chamber is filled with anhydrous sodium sulfate packing, and the other Silica gel packing is installed in the lattice packing cavity. The utility model can achieve the effect of sample drying and purification by passing through the column once, and has the advantages of simplifying sample processing steps and saving sample processing time. The solid phase extraction column has the characteristics of simple structure, convenient use and low cost.

Description

A solid-phase extraction column for the determination of benzo[α]pyrene in cigarette smoke

technical field

The utility model belongs to analytical chemistry facilities, in particular to a solid-phase extraction column used in the analysis of benzo[α]pyrene in cigarette smoke.

Background technique

Benzo[α]pyrene is the most important carcinogen in fused-ring aromatic hydrocarbons; although the amount of benzo[α]pyrene in cigarette smoke is extremely small, its carcinogenic effect is significantly higher than that of other fused-ring aromatic hydrocarbons. [α]pyrene has been listed as one of the seven indicators for cigarette safety evaluation in my country. At present, the determination methods of benzo[α]pyrene include thin-layer chromatography (TLC), gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC), etc. It is meaningful to find a simple and rapid method for the determination of benzo[α]pyrene.

my country's tobacco industry standard uses GC-MS method to determine benzo[α]pyrene in cigarette smoke. The operation of the method is: use a smoking machine to collect the total particulate matter of the flue gas, then extract the benzo[α]pyrene in the total particulate matter with cyclohexane, dry the extract through anhydrous sodium sulfate, and use solid-phase extraction The column was cleaned up, and then determined by GC-MS. This method has many and complicated processing steps, and sample drying and sample purification need to be carried out separately, so the processing time is longer.

Contents of the invention

The purpose of the utility model is to provide a solid-phase extraction column for the deficiencies of the prior art. The solid-phase extraction column can achieve the effect of sample drying and purification by passing through the column once, and has the advantages of simplifying sample processing steps and saving sample processing. advantage of time.

The technical solution adopted to realize the above-mentioned purpose of the utility model is: the solid-phase extraction column includes a column cavity and a column cap, and a sieve plate is respectively arranged at the upper, middle and lower positions in the column cavity, which are separated into two packing chambers, one grid Anhydrous sodium sulfate filler is installed in the filler cavity, and silica gel filler is installed in the other filler cavity.

The two ports of the column cavity are threaded, and the column cap is a screw cap with corresponding threads, and the two are connected by threads to compress the filler in the column cavity.

The inner diameter of the column cavity is 1 cm, the length is 2 cm, and the volume occupied by the anhydrous sodium sulfate filler in the column cavity is 25% of that of the silica gel filler.

The beneficial effects of the utility model are: the column cavity is divided into two grids by a sieve plate, and two kinds of fillers can be loaded, and the effect of sample drying and purification can be achieved by passing through the column once, and the sample is simplified compared with the conventional solid phase extraction column. The processing steps save sample processing time; the column cavity and column cap adopt a movable structure of screw connection, which can be disassembled and refilled after use, which can save costs and reduce costs, and the solid phase extraction column is very convenient to fill; solid phase extraction The column material can be made of FEP material, which has good heat resistance, chemical resistance and solvent resistance, and also has excellent permeability resistance, which can effectively prevent the dissolution or aging of impurities in the solid phase extraction column during use , improving the safety and detection stability of the utility model product.

Description of drawings

Fig. 1 is the structural representation of the solid phase extraction column of the present invention.

Among them, 1-column cavity; 2-column cap; 3-sieve plate; 4-anhydrous sodium sulfate packing; 5-silica gel packing.

Detailed ways

See Figure 1, the material of this solid phase extraction column is all made of FEP, the diameter of the column is 1 cm, the length is 2 cm, the openings at both ends of the column cavity 1 are processed with threads, and the column cap 2 is processed with corresponding threads . The upper, middle and lower positions of the column cavity 1 are respectively provided with a sieve plate 3, which is divided into two grids by the sieve plate, and anhydrous sodium sulfate filler 4 is placed on the upper end of the column, and the top sieve plate 3 is installed after compaction. Tighten the column cap 2; then put the silica gel filler 5 into the lower port of the column cavity 1, compact it, install the sieve plates 3 at the upper and lower ports, tighten the column cap 2, and the solid phase extraction column is loaded. When the sample is extracted, the sample solution enters from the upper end (the end filled with anhydrous sodium sulfate), first passes through the anhydrous sodium sulfate layer to dry, and then flows through the silica gel layer for purification. The flow rate of the sample passing through the column can be 10-25mL/min. After passing through the column, the eluent also enters from the upper end for elution, and the elution flow rate can be 5-15mL/min.

Claims (3)

1. one kind is used for the solid-phase extraction column that cigarette smoke benzo [α] pyrene is measured; Comprise post cavity and cap; It is characterized in that: the position, upper, middle and lower respectively is provided with a sieve plate in the post cavity; Be separated into two lattice packing cavities, dress anhydrous sodium sulfate filler is adorned silica filler in another lattice packing cavity in the lattice packing cavity.

2. be used for the solid-phase extraction column that cigarette smoke benzo [α] pyrene is measured by claim 1 is described, it is characterized in that: the two-port of post cavity is threaded, and cap is the spiral cover with corresponding screw thread, the two through be threaded and the hold-down column cavity in filler.

3. by the described solid-phase extraction column that is used for cigarette smoke benzo [α] pyrene mensuration of claim 1, it is characterized in that: the internal diameter of post cavity is 1 cm, and length is 2 cm, and anhydrous sodium sulfate filler shared volume in the post cavity is 25% of a silica filler.

Images