[go: up one dir, main page]

CN201605273U - 半贴壁细胞培养皿 - Google Patents

半贴壁细胞培养皿 Download PDF

Info

Publication number
CN201605273U
CN201605273U CN 200920352216 CN200920352216U CN201605273U CN 201605273 U CN201605273 U CN 201605273U CN 200920352216 CN200920352216 CN 200920352216 CN 200920352216 U CN200920352216 U CN 200920352216U CN 201605273 U CN201605273 U CN 201605273U
Authority
CN
China
Prior art keywords
dish
ware
liquid
cell culture
semi
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN 200920352216
Other languages
English (en)
Inventor
邹绚
沈荐
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN 200920352216 priority Critical patent/CN201605273U/zh
Application granted granted Critical
Publication of CN201605273U publication Critical patent/CN201605273U/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/18—Flow directing inserts
    • C12M27/22—Perforated plates, discs or walls

Landscapes

  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

本实用新型涉及一种适于半贴壁细胞培养，减少换液过程中细胞流失的细胞培养装置。其由皿盖、内皿和外皿组成，皿盖直径大于外皿直径大于内皿直径，内皿的底壁有两个通水孔，可使液体在内皿和外皿间流动。进行细胞换液时，缓慢提起附有半贴壁细胞的内皿，内皿中的液体会自通水孔流入到外皿中。当外皿更换液体后，重新缓慢放回内皿，外皿中的液体会自通水孔进入内皿。此装置使半贴壁细胞的换液过程更简单、便捷，不会造成大的震动和水流冲击，有效避免了半贴壁细胞在换液过程中的流失。

Description

半贴壁细胞培养皿
一、技术领域
本实用新型涉及一种细胞培养装置，尤其是适于半贴壁细胞培养，减少换液过程中细胞流失的半贴壁细胞培养皿。
二、背景技术
目前，公知的细胞培养皿，都是底面积不同的圆柱状容器。对细胞进行换液时，需通过直接倾倒或用移液器抽吸的方法更换皿中液体。在此过程中，水流会对皿底有一定的冲击，这对一般的贴壁细胞不会造成影响。但半贴壁细胞的贴壁附着能力较差，轻微的震动和水流的冲击都会使其离壁悬浮，随换液过程而流失，最终导致实验出现误差。
三、发明内容
为了克服现有培养皿在换液过程中易造成半贴壁细胞流失的不足，本实用新型提供一种半贴壁细胞培养皿，该培养皿使半贴壁细胞的换液过程更简便，且不易造成细胞的流失。
本实用新型解决其技术问题所采用的技术方案是：半贴壁细胞培养皿由皿盖、内皿和外皿组成，其材料可选用玻璃或聚苯乙烯等。皿盖直径大于外皿直径大于内皿直径，内皿直径可为35毫米、60毫米、100毫米、150毫米等多种规格。内皿的两侧各有一弧形支架，支架的底边略低于内皿的底壁，即单独放置内皿时，支架可以托起内皿，使其底壁不接触桌面。在支架的外侧各有一扶手，可供手抓提起内皿用。在内皿的底壁有两个通水孔，可使液体在内皿和外皿间流动。在每个通水孔的边缘有高约2毫米的孔壁，可防止通水孔周边的细胞在生长过程中沿通水孔掉出内皿。
换液时缓慢提起附有半贴壁细胞的内皿，内皿中的液体会自通水孔流入到外皿中，移开内皿，倾倒出外皿内的液体，再向外皿中加入新的液体，重新缓慢放回内皿，外皿中的液体会自通水孔进入内皿。
本实用新型的有益效果是：使半贴壁细胞的换液过程更简单、便捷，不会造成大的震动和水流冲击，有效避免了半贴壁细胞在换液过程中的流失。
四、附图说明
下面结合附图和实施例对本实用新型进一步说明。
图1是本实用新型的立体图。
图2是本实用新型组合后的纵剖面构造图。
图中1.皿盖；
2.内皿，其上有：2-1.支架，2-2.扶手，2-3.通水孔，2-4.孔壁；
3.外皿。
五、具体实施方式
向半贴壁细胞培养皿中接种细胞时，掀开皿盖(1)，把含有半贴壁细胞的悬浮液滴入到套有外皿(3)的内皿(2)中，然后盖回皿盖(1)，放在适宜条件下培养。一段时间后，大部分细胞会自然沉降黏附于内皿(2)的底壁上。内皿(2)底壁的通水孔(2-3)的边缘有高约2毫米的孔壁(2-4)，其可以有效防止通水孔(2-3)周边已贴壁生长的细胞因增殖沿通水孔(2-3)掉出内皿(2)。
待细胞贴壁培养一段时间需要换液时，不必掀开皿盖(1)，而直接用手扶住内皿(2)上的扶手(2-2)，上提取出盖有皿盖(1)的内皿(2)，放置于桌面上，因支架(2-1)的底边略低于内皿(2)的底壁，故内皿(2)的底壁不会接触到桌面而污染。倒空外皿(3)中的液体，加入新的平衡液或培养基，重新放回盖有皿盖(1)的内皿(2)。
在换液过程中，液体可以通过通水孔(2-3)平稳的在内皿(2)和外皿(3)中流动，使半贴壁细胞免受冲击而脱落。

Claims (2)

1.一种半贴壁细胞培养皿，其特征在于：由皿盖、内皿和外皿组成，皿盖直径大于外皿直径大于内皿直径。
2.根据权利要求1所述的半贴壁细胞培养皿，其特征在于：内皿的两侧各有一弧形支架，支架的外侧各有一扶手；内皿的底壁有两个通水孔，每个通水孔的边缘均有高约2毫米的孔壁。
CN 200920352216 2009-12-31 2009-12-31 半贴壁细胞培养皿 Expired - Fee Related CN201605273U (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200920352216 CN201605273U (zh) 2009-12-31 2009-12-31 半贴壁细胞培养皿

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 200920352216 CN201605273U (zh) 2009-12-31 2009-12-31 半贴壁细胞培养皿

Publications (1)

Publication Number Publication Date
CN201605273U true CN201605273U (zh) 2010-10-13

Family

ID=42949981

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 200920352216 Expired - Fee Related CN201605273U (zh) 2009-12-31 2009-12-31 半贴壁细胞培养皿

Country Status (1)

Country Link
CN (1) CN201605273U (zh)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103409319A (zh) * 2013-07-24 2013-11-27 中国人民解放军第三军医大学第一附属医院 肿瘤细胞失巢培养器
CN109642200A (zh) * 2016-08-18 2019-04-16 普兰德有限两合公司 细胞培养插入皿和用于培养细胞的装置

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103409319A (zh) * 2013-07-24 2013-11-27 中国人民解放军第三军医大学第一附属医院 肿瘤细胞失巢培养器
CN109642200A (zh) * 2016-08-18 2019-04-16 普兰德有限两合公司 细胞培养插入皿和用于培养细胞的装置
US11566216B2 (en) 2016-08-18 2023-01-31 Brand Gmbh + Co Kg Cell culture insert and device for cultivating cells

Similar Documents

Publication Publication Date Title
CN202450098U (zh) 一种用于组织块培养细胞的培养瓶
CN201695031U (zh) 分格玻璃底培养皿
WO2008123741A1 (en) Cell culture dish for the embryoid body formation from embryonic stem cells
CN201605273U (zh) 半贴壁细胞培养皿
CN201793567U (zh) 一种微生物培养装置
CN104450505B (zh) 一种组织块法培养皿
CN204939492U (zh) 一种多功能多孔细胞培养皿
CN217025936U (zh) 一种细胞培养瓶
CN206266633U (zh) 一种细胞间接共培养支架
CN205774572U (zh) 一种用于成熟脂肪细胞天花板培养的培养皿
CN203048938U (zh) 新型培养皿
CN202671544U (zh) 减震培养皿
CN203563488U (zh) 一种植物液体培养承载架
CN202415564U (zh) 双面细胞培养瓶
CN207685256U (zh) 一种具有良好密封作用的微生物检测用培养皿
CN202017021U (zh) 一种微生物培养装置
CN208869583U (zh) 一种培养皿的固定装置
CN202912968U (zh) 医用细胞培养皿
CN203187677U (zh) 一种可使培养基厚度一致的培养皿
CN210560492U (zh) 一种干细胞分层共培养的装置
CN202773641U (zh) 水培植物容器
CN202465708U (zh) 一种改进型细胞培养皿
CN211771363U (zh) 一种细胞培养和检测用培养板
CN203238274U (zh) 组织块培养皿
CN204676100U (zh) 适用于细胞、组织和生物支架共培养的六孔共培养板

Legal Events

Date Code Title Description
C14 Grant of patent or utility model
GR01 Patent grant
C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20101013

Termination date: 20101231