CN207567235U - Random pcr system - Google Patents
Random pcr system Download PDFInfo
- Publication number
- CN207567235U CN207567235U CN201721466659.1U CN201721466659U CN207567235U CN 207567235 U CN207567235 U CN 207567235U CN 201721466659 U CN201721466659 U CN 201721466659U CN 207567235 U CN207567235 U CN 207567235U
- Authority
- CN
- China
- Prior art keywords
- optical
- light
- optical excitation
- pcr
- slot
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000003287 optical effect Effects 0.000 claims abstract description 64
- 230000005284 excitation Effects 0.000 claims abstract description 33
- 238000001514 detection method Methods 0.000 claims abstract description 29
- 238000006243 chemical reaction Methods 0.000 claims abstract description 20
- 239000013307 optical fiber Substances 0.000 claims abstract description 4
- 238000010438 heat treatment Methods 0.000 claims description 11
- 239000000919 ceramic Substances 0.000 claims description 3
- 229910052736 halogen Inorganic materials 0.000 claims description 3
- 150000002367 halogens Chemical class 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 claims description 3
- 229910052721 tungsten Inorganic materials 0.000 claims description 3
- 239000010937 tungsten Substances 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000010586 diagram Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 3
- 238000004891 communication Methods 0.000 description 2
- 238000001506 fluorescence spectroscopy Methods 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000003028 elevating effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The utility model discloses a kind of random PCR system, including electric-control system, PCR temperature control systems, optical excitation detecting system；PCR temperature control systems include multiple independent specimen cup reaction warehouses, and the specimen cup reaction warehouse is by being arranged on the indoor temperature control device in storehouse and the slot of PCR reaction vessels being received to form；Optical excitation detecting system is made of carrier, optical excitation device and optical detection apparatus；Carrier is made of price fixing and Moving plate, multiple light receiving units for being used to receive incident light and emergent light is provided on Moving plate, each light receiving unit passes through incident optical and the output optical fiber respectively and a corresponding slot forms input path and emitting light path；Optical excitation device and optical detection apparatus are arranged on price fixing.The utility model advantage is while flexibility is ensured, an optical excitation detecting system is greatly saved the manufacture cost of optical system.
Description
Technical field
The utility model is related to clinical PCR detection, more particularly, to random PCR system.
Background technology
PCR system, is a kind of in DNA amplification reaction, and each PCR is detected with fluorescent chemical
(PCR：Polymerase Chain Reaction) cycle after product total amount method.PCR system is then for providing not equality of temperature
The platform of degree condition and the fluorescence of monitoring cyclic process, is connected with real-time device and collects fluorescence data, fluorescence number by computer
It is graphically shown according to by the real-time analysis software of exploitation, raw fluorescence data is drawn into fluorescence intensity relative to following
The chart of number of rings is used to be analyzed.
With universal and PCR detection projects the expansion of round pcr clinically, the clinical flexibility to PCR detections is opened
There is certain demand in beginning.There are mainly two types of current PCR instruments, and one kind is porous（96 holes）PCR instrument, multiple sample slots share warm lifting
There is the defects of needing program consistent with an amplified reaction in system and optical excitation detecting system, can not flexible Application；Separately
One kind is single hole modularization PCR instrument, each sample slot has independent warm elevating control and optical excitation detecting system, has
Great flexibility, but it is configured an optical excitation and detecting system as well as each sample slot, and PCR instrument
Main manufacture cost is optical excitation and detecting system, this results in existing PCR instrument manufacture with high costs.
Invention content
The utility model aim is to provide a kind of random PCR system, realizes random detection and reduce manufacture cost.
To achieve the above object, the utility model takes following technical proposals：
Random PCR system described in the utility model, including electric-control system, PCR temperature control systems, optical excitation detection system
System；The PCR temperature control systems include multiple independent specimen cup reaction warehouses, and the specimen cup reaction warehouse is indoor by being arranged on storehouse
Temperature control device and the slot composition for receiving PCR reaction vessels；The optical excitation detecting system is by carrier, optical excitation device
It is formed with optical detection apparatus；The carrier is made of price fixing and Moving plate driven by stepper motors, is set on the price fixing
Have it is multiple for receiving the light receiving units of incident light and emergent light, each light receiving unit respectively by incident optical and
The output optical fiber forms input path and emitting light path with corresponding one slot；The optical excitation device and optical detection
Device is arranged on the Moving plate, and optical excitation device is by light source, the first lens of incident light, incident optical filter and incident light second
Lens are formed；Optical detection apparatus is by the first lens of emergent light, outgoing optical filter, the second lens of emergent light and optical detection elements
It forms.
The temperature control device is by being fixed on the indoor turbofan in the storehouse, the electrical heating being fixed on slot wall member
Part and temperature sensor composition.
The air outlet of the turbofan is set against the slot wall, and the electrical heating elements are fixed on the bottom of slot
Wall, the temperature sensor are fixed on the side wall of slot.
The electrical heating elements are ceramic heating flake, Peltier or spiral resistance wire.
The light source of the optical excitation device is LED, halogen tungsten lamp, laser or solid state light emitter.
The optical detection elements are photodiode, photomultiplier, CCD or the optical detection component based on CPM.
The utility model advantage is by the specimen cup reaction warehouse of multiple independent temperatures and an optical excitation detection system
System composition, therefore so that each specimen cup reaction warehouse can provide different temperature change environment, for different temperatures is needed to change
The sample of environment carries out amplification and provides condition, and while flexibility is ensured, an optical excitation detecting system is also great
Save the manufacture cost of optical system.
Description of the drawings
Fig. 1 is the structure diagram of the utility model.
Fig. 2 is the structure diagram of specimen cup reaction warehouse described in the utility model.
Fig. 3 is the structure diagram of optical excitation device described in the utility model.
Fig. 4 is the structure diagram of optical detection apparatus described in the utility model.
Fig. 5 is the optics schematic diagram of optical excitation detecting system described in the utility model.
Fig. 6 is the control circuit functional block diagram of the utility model.
Specific embodiment
Elaborate below in conjunction with the accompanying drawings to the embodiment of the present invention, the present embodiment using technical solution of the present invention before
It puts and is implemented, give detailed embodiment and specific operating process, but protection scope of the present invention is not limited to down
State embodiment.
As shown in Figs. 1-5, random PCR system described in the utility model, including electric-control system, PCR temperature control systems, optics
Excitation detection system；PCR temperature control systems include ten（Certainly, can increase as needed）Independent specimen cup reaction warehouse 1,
Specimen cup reaction warehouse 1 is made of the temperature control device and the slot 4 of receiving PCR reaction vessels 3 being arranged in bin 2；Temperature control device
By the turbofan 5 being fixed in bin 2, the ceramic heating flake 6 being fixed on 4 bottom wall of slot（Or Peltier, spiral resistance wire
Wait electrical heating elements）And the temperature sensor 7 being fixed on 4 side wall of slot forms, the air outlet of turbofan 5 is against slot
Wall is set.
As shown in Figure 2,3, 4, optical excitation detecting system is by 9 structure of carrier, optical excitation device 8 and optical detection apparatus
Into；Carrier is made of price fixing 10 and the Moving plate driven by stepper motor 11 12；Price fixing 10 and Moving plate 12 are coaxially set, price fixing
Be provided on 10 it is multiple for receive incident light and emergent light incident light receiving unit 13, outgoing light receiving unit 14, each
Incident light receiving unit 13 forms input path by incident optical 15 with a corresponding slot 4 respectively, and each emergent light connects
It receives unit 14 and emitting light path is formed with a corresponding slot 4 by the output optical fiber 16 respectively.
Optical excitation device 8 and optical detection apparatus 9 are arranged on Moving plate 12, as shown in Fig. 3,4,5, optical excitation device
8 are made of light source 17, the first convex lens of incident light 18, incident optical filter 19 and the second convex lens of incident light 20；Optical detection fills
9 are put to be made of the first convex lens of emergent light 21, outgoing optical filter 22, the second convex lens of emergent light 23 and optical detection elements 24.
Light source 17 selects LED, and halogen tungsten lamp, laser or solid state light emitter also may be selected；The color of light source 17 is optional as needed
It selects a variety of；Optical detection elements 24 select photodiode, photomultiplier, CCD or the optical detection component based on CPM.
The optical excitation device 8 and optical detection apparatus 9 being arranged on Moving plate 12 can set multipair, every a pair of optics
17 color of light source of excitation apparatus 8 is different, for example, six pairs, in this way, when needing different colored light sources, you can easily select
The optical excitation device 8 of 17 color of light source needed for satisfaction.
As shown in fig. 6, the control circuit principles illustrated of the utility model is as follows：
The power supply of MCU is provided by 24V by conversion circuit；CAN transceiver arrives CAN interface again by CAN level conversions, bears
Duty carries out CAN communication with host；UART arrives RS232 interface again by serial ports conversion chip, is responsible for the serial communication with host；Two
Road temperature sensor interface, a temperature sensor for connecing load blocks, one connects environment temperature sensor；IO output controls,
Exported using PWM, carry out the control of motor-driven duty cycle of switching, and with electric current commutation control, load voltage it is defeated
Inbound port range is 12 ~ 24V, output termination heating/refrigerating load.
Claims (6)
1. a kind of random PCR system, including electric-control system, PCR temperature control systems, optical excitation detecting system；It is characterized in that：Institute
It states PCR temperature control systems and includes multiple independent specimen cup reaction warehouses, the specimen cup reaction warehouse is by being arranged on the indoor temperature control in storehouse
Device and the slot composition for receiving PCR reaction vessels；The optical excitation detecting system is by carrier, optical excitation device and light
Detection device is learned to form；The carrier is made of price fixing and Moving plate driven by stepper motors, is provided on the price fixing more
A light receiving unit for being used to receive incident light and emergent light, each light receiving unit pass through incident optical and outgoing respectively
Optical fiber forms input path and emitting light path with corresponding one slot；The optical excitation device and optical detection apparatus
It is arranged on the Moving plate, optical excitation device is by light source, the first lens of incident light, the second lens of incident optical filter and incident light
It forms；Optical detection apparatus is by the first lens of emergent light, outgoing optical filter, the second lens of emergent light and optical detection elements structure
Into.
2. random PCR system according to claim 1, it is characterised in that：The temperature control device is by being fixed on the bin
Interior turbofan, the electrical heating elements being fixed on the slot wall and temperature sensor composition.
3. random PCR system according to claim 2, it is characterised in that：The air outlet of the turbofan is described in
Slot wall is set, and the electrical heating elements are fixed on the bottom wall of slot, and the temperature sensor is fixed on the side wall of slot.
4. random PCR system according to claim 3, it is characterised in that：The electrical heating elements are ceramic heating flake, pa
You paste or spiral resistance wire.
5. random PCR system according to claim 1, it is characterised in that：The light source of the optical excitation device for LED,
Halogen tungsten lamp, laser or solid state light emitter.
6. random PCR system according to claim 1, it is characterised in that：The optical detection elements for photodiode,
Photomultiplier, CCD or the optical detection component based on CPM.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721466659.1U CN207567235U (en) | 2017-11-07 | 2017-11-07 | Random pcr system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721466659.1U CN207567235U (en) | 2017-11-07 | 2017-11-07 | Random pcr system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207567235U true CN207567235U (en) | 2018-07-03 |
Family
ID=62693829
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721466659.1U Active CN207567235U (en) | 2017-11-07 | 2017-11-07 | Random pcr system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207567235U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107653187A (en) * | 2017-11-07 | 2018-02-02 | 安图实验仪器(郑州)有限公司 | Random pcr system |
-
2017
- 2017-11-07 CN CN201721466659.1U patent/CN207567235U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107653187A (en) * | 2017-11-07 | 2018-02-02 | 安图实验仪器(郑州)有限公司 | Random pcr system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107653187A (en) | Random pcr system | |
| CN111344552B (en) | Multiplexed Bioassay Device with Electronic Readout | |
| EP2876432A1 (en) | Light measurement apparatus for reaction vessel, and light measurement method | |
| EP1866631B1 (en) | Multiplex fluorescence detection device having fiber bundle coupling multiple optical modules to a common detector | |
| US20100105035A1 (en) | Electroluminescent-based fluorescence detection device | |
| CN105802848A (en) | Recombinase-mediated isothermal nucleic acid amplification reaction real-time detection apparatus | |
| CN110791423A (en) | Nucleic acid detection device, method and system | |
| CN1245449A (en) | Reaction vessels | |
| JP2016515207A (en) | Apparatus for detecting signal emission from multiple fluorescent sources | |
| CN101932927A (en) | Integrated device for carrying out and monitoring chemical reactions | |
| CN106929388A (en) | A kind of real-time fluorescence quantitative PCR instrument | |
| CN207567235U (en) | Random pcr system | |
| CN212581920U (en) | Detection system for detection of alcohol metabolism-related genes | |
| CN104120078A (en) | Real-time fluorescence PCR (Polymerase Chain Reaction) amplification instrument | |
| CN211847961U (en) | Nucleic acid detection device and system | |
| JP2023532054A (en) | Ultra-fast PCR thermal cycler | |
| CN212581870U (en) | Nucleic acid extraction purification and amplification detection system | |
| CN110361552B (en) | Centrifugal portable microfluidic analysis device and application method thereof | |
| CN107543806A (en) | Multi-channel fluorescence detection system and method thereof | |
| CN102586098B (en) | Real-time fluorescent PCR (polymerase chain reaction) working system of space-oriented micro-volume unit | |
| CN106497777A (en) | A kind of self-checking system of real-time fluorescence quantitative PCR instrument and its self checking method | |
| CN213475927U (en) | Real-time fluorescence PCR detection device with double modules operating independently | |
| CN107976402A (en) | It is a kind of based on the modularization bio-analysis system of droplet array chip and its application | |
| CN219455949U (en) | A Fluorescent Detection Mechanism for Molecular Diagnostic Devices | |
| CN113549698A (en) | Primer group, probe group, detection method and system for detecting alcohol metabolism related gene |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |