CN2067424U - DNA augmentor for polymerase chain reaction - Google Patents
DNA augmentor for polymerase chain reaction Download PDFInfo
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- CN2067424U CN2067424U CN 90205989 CN90205989U CN2067424U CN 2067424 U CN2067424 U CN 2067424U CN 90205989 CN90205989 CN 90205989 CN 90205989 U CN90205989 U CN 90205989U CN 2067424 U CN2067424 U CN 2067424U
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- 238000003752 polymerase chain reaction Methods 0.000 title abstract description 3
- 239000007787 solid Substances 0.000 claims abstract description 14
- 238000010438 heat treatment Methods 0.000 claims abstract description 12
- 239000004065 semiconductor Substances 0.000 claims abstract description 6
- 229910000838 Al alloy Inorganic materials 0.000 claims abstract description 3
- 229910052751 metal Inorganic materials 0.000 claims description 7
- 239000002184 metal Substances 0.000 claims description 7
- 238000005057 refrigeration Methods 0.000 claims description 2
- 238000010367 cloning Methods 0.000 claims 1
- 230000000994 depressogenic effect Effects 0.000 claims 1
- 239000012472 biological sample Substances 0.000 abstract description 14
- 238000000034 method Methods 0.000 abstract description 6
- 238000001816 cooling Methods 0.000 abstract description 3
- 230000004544 DNA amplification Effects 0.000 abstract description 2
- 238000012423 maintenance Methods 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 4
- 102000053602 DNA Human genes 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 230000009347 mechanical transmission Effects 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
聚合酶链式反应DNA扩增仪，属于分析仪器类。
本实用新型包括一台单板计算机、电源装置和加热装置，外罩铝合金外壳，面板上有操作键盘和数码显示屏。在加热装置中，采用了由半导体温差致冷组件构成的固体加热器对在载物台上的生物样品不需要移动便可完成在三种不同的恒温下轮流加热的过程，所以结构特别简单，无噪音，耐振动，不需要经常维修。
A polymerase chain reaction DNA amplification instrument belongs to the category of analytical instruments.
The utility model comprises a single-board computer, a power supply device and a heating device, an outer cover of an aluminum alloy shell, and an operation keyboard and a digital display screen on the panel. In the heating device, a solid heater composed of a semiconductor thermo-cooling component is used to complete the process of heating the biological samples on the stage at three different constant temperatures in turn without moving, so the structure is particularly simple. No noise, vibration resistance, no need for frequent maintenance.
Description
本实用新型涉及一种在生物实验技术中使用的聚合酶链式反应DNA（脱氧核糖核酸）扩增仪，属于分析仪器类。The utility model relates to a polymerase chain reaction DNA (deoxyribonucleic acid) amplification instrument used in biological experiment technology, which belongs to the analysis instrument category.
一项从微量生物样品中采用体外扩增的方式获取大量特定遗传物质的技术，是利用DNA聚合酶依赖于DNA模板的特征，模拟体内的复制过程，在附加的一对引物之间诱发聚合反应。这个工作过程基于在三种不同温度下变性、退火、延伸循环完成的。这样，实验需要有三种不同温度的恒温装置。同一个生物样品分别在这三种恒温下按一定的次序轮流加热，而且在每一种恒温下仃留的时间不等，每轮流一回，为一次循环，有时在每次循环后，三种不同恒温的设定值还要递增，这样的循环要进行30－50次。若实验另外一种生物样品时，则三种恒温和在每种恒温下仃留的时间又与前一种有所不同。这样的操作目前是用由单板计算机控制的机械传动机构把同一个生物样品按一定次序轮流浸泡到水温不同的三个恒温水浴槽内，用水浴式加温法来完成。水浴式加温法有这样的问题，即水浴槽内的水因受到加热要蒸发一部分，特别是在接近沸腾时，蒸发更快，需要经常补充新水；生物样品在由一个水浴槽移到另一个水浴槽的过程中，可能把残留的水滴到仪器内；另外，机械传动部分是由电动机、凸轮体、摇杆和承物架组成的提取机构，它结构复杂、起动频繁、有噪音，长时间工作，容易出现机械故障。A technology for obtaining a large amount of specific genetic material from a small amount of biological samples by means of in vitro amplification, which uses the characteristics of DNA polymerase to rely on DNA templates, simulates the replication process in vivo, and induces a polymerization reaction between an additional pair of primers . This working process is based on three cycles of denaturation, annealing, and extension at three different temperatures. Thus, the experiment requires a thermostat with three different temperatures. The same biological sample is heated at these three constant temperatures in a certain order in turn, and the time of staying at each constant temperature is different. Each turn is a cycle, and sometimes after each cycle, the three The setting values of different constant temperatures will also be incremented, and such a cycle will be carried out 30-50 times. If another biological sample is tested, the three constant temperatures and the time of staying at each constant temperature are different from the previous one. Such an operation is currently done by using a mechanical transmission mechanism controlled by a single-board computer to soak the same biological sample in turn in three constant temperature water baths with different water temperatures in a certain order, and complete it with a water bath heating method. The water bath heating method has the problem that part of the water in the water bath will evaporate due to heating, especially when it is close to boiling, the evaporation is faster, and new water needs to be added frequently; biological samples are moved from one water bath to another. In the process of a water bath, the residual water may drop into the instrument; in addition, the mechanical transmission part is an extraction mechanism composed of a motor, a cam body, a rocker and a supporting frame. It has a complex structure, frequent starts, and noise. Working hours, prone to mechanical failure.
本实用新型的目的是要提供一种由半导体温差致冷组件构成的固体加热器，利用对其改变供电电流的大小和方向可使它产生加温或致冷效果的特性，对载物台上的生物样品分别在三种恒温下轮流加热。The purpose of this utility model is to provide a solid heater composed of semiconductor thermorefrigeration components, which can produce heating or cooling effects by changing the magnitude and direction of the power supply current to it. The biological samples were heated alternately at three constant temperatures.
本实用新型的目的是这样实现的：一个由半导体温差致冷组件构成的固体加热器由电源装置供电，用单板计算机自动控制电源装置供电电流的大小和方向，从而可改变固体加热器的温度。固体加热器被导热粘合剂粘接到由一块金属板制成的载物台的台底，在载物台上的竖向槽内装入放有生物样品的试管，被固体加热器加热。单板计算机根据予置的温度、时间、循环次数和载物台上温度传感器输入的温度数据来控制电源装置供电电流的大小、方向和时间，从而实现对生物样品在三种不同恒温下定时和轮流加热的目的。The purpose of this utility model is achieved in this way: a solid heater composed of a semiconductor thermoelectric refrigeration component is powered by a power supply, and the size and direction of the power supply current of the power supply are automatically controlled by a single-board computer, thereby changing the temperature of the solid heater . The solid heater is bonded to the bottom of the stage made of a metal plate by a heat-conducting adhesive, and the test tubes with biological samples are placed in the vertical slots on the stage and heated by the solid heater. The single-board computer controls the size, direction and time of the power supply current of the power supply device according to the preset temperature, time, number of cycles and the temperature data input by the temperature sensor on the stage, so as to realize the timing and processing of biological samples at three different constant temperatures. The purpose of heating in turn.
本实用新型由于采用了由半导体温差致冷组件构成的固体加热器，装有生物样品的试管放在载物台的槽内，不需要做任何移动便可接受固体加热器在不同的时间内提供的不同的恒定温度，所以结构特别简单，无噪音，耐振动，不需要经常维修。Because the utility model adopts a solid heater composed of a semiconductor thermorefrigeration component, the test tube containing the biological sample is placed in the groove of the stage, and can be provided by the solid heater at different times without any movement. Different constant temperature, so the structure is very simple, no noise, vibration resistance, no need for frequent maintenance.
本实用新型的结构及工作情况结合实施例及附图说明如下。Structure and working conditions of the present utility model are described as follows in conjunction with embodiment and accompanying drawing.
图1是本实用新型的剖面图；Fig. 1 is a sectional view of the utility model;
图2是本实用新型的单板计算机程序流程图；Fig. 2 is a single-board computer program flow chart of the present utility model;
本实用新型的一个实施例命名为PCR－90B型DNA扩增仪，它由单板计算机（1）、电源装置（2）和加热装置（9）构成，外罩铝合金外壳（4），顶部有保温罩（6），正面有操作键盘和数码显示屏（5）监控本扩增仪的工作情况。加热装置（9）是由半导体温差致冷组件构成的固体加热器（8）、金属散热片（3）和载物台（7）构成的；固体加热器（8）被导热粘合剂粘接到载物台（7）的台底，紧贴固体加热器（8）的底部固定安装散热片（3）；载物台（7）由一块金属板制成，在这块金属板上均匀分布20－80个或更多个底部呈半圆形凹陷的竖向槽（10），在这块金属板上还装有一支检测温度的温度传感器（11）。One embodiment of the utility model is named PCR-90B type DNA amplification instrument, which is composed of a single board computer (1), a power supply (2) and a heating device (9), with an aluminum alloy shell (4) on the outer cover, and a The heat preservation cover (6) has an operation keyboard and a digital display screen (5) on the front to monitor the working conditions of the amplification instrument. The heating device (9) is composed of a solid heater (8) composed of semiconductor thermoelectric cooling components, a metal heat sink (3) and a stage (7); the solid heater (8) is bonded by a thermally conductive adhesive to the bottom of the stage (7), and fix the heat sink (3) close to the bottom of the solid heater (8); the stage (7) is made of a metal plate, and the 20-80 or more vertical grooves (10) whose bottoms are semicircular depressions, and a temperature sensor (11) for detecting temperature is also housed on this metal plate.
实验时，先把装有生物样品的试管放在载物台（7）的每个槽（10）内，再用保温罩（6）盖在载物台（7）上。开机时，单板计算机（1）先清零；再予置三个不同的温度值A、B、C及在这三个温度下的仃留时间tA、tB、tC；予置循环次数计数器D；下一步是温度传感器（11）将载物台（7）的温度值输入单板计算机（1）与设定温度值C比较，不满足C值时，单板计算机（1）输出加电信号给 电源装置（2），电源装置（2）输出加电电流给 固体加热器（8），加电电流的大小和方向根据温度值C的大小，由单板计算机（1）进行调节，载物台（7）的温度达到C值，并由单板计算机（1）控制温度C值恒定，生物样品在载物台（7）上受温度C值的加热，直到仃留时间tC结束时，单板计算机（1）再调节电源装置（2）的加电电流，使载物台（7）的温度为A值，并由单板计算机（1）控制温度A值恒定，生物样品在载物台（7）的温度为B值，并由单板计算机（1）控制温度B值恒定，生物样品在载物台（7）上受温度B值的加热，直到仃留时间tB结束时，单板计算机（1）再调节电源装置（2）的加电电流，使载物台（7）上受温度A值的加热，直到仃留时间tA结束时，完成一次循环，计数器D减1，接着再进入下一次循环，直到计数器D为零时，实验结束。During experiment, earlier the test tube that biological sample is housed is placed in each groove (10) of stage (7), then covers on stage (7) with insulation cover (6). When starting up, the single board computer (1) is first cleared; then three different temperature values A, B, C and the dwell time t A , t B , t C at these three temperatures are preset; the preset cycle The number of times counter D; the next step is that the temperature sensor (11) inputs the temperature value of the stage (7) into the single-board computer (1) and compares it with the set temperature value C. When the value of C is not satisfied, the single-board computer (1) outputs The power-on signal is sent to the power supply unit (2), and the power supply unit (2) outputs the power-on current to the solid heater (8). The size and direction of the power-on current are adjusted by the single-board computer (1) according to the temperature value C , the temperature of the stage (7) reaches the C value, and the temperature C value is controlled by the single-board computer (1) to be constant, and the biological sample is heated by the temperature C value on the stage (7) until the residence time t C At the end, the single-board computer (1) adjusts the power supply current of the power supply unit (2), so that the temperature of the stage (7) is A value, and the temperature A value is controlled by the single-board computer (1). The biological sample The temperature on the stage (7) is B value, and the temperature B value is controlled by the single board computer (1), and the biological sample is heated by the temperature B value on the stage (7) until the residence time t B At the end, the single-board computer (1) adjusts the power supply current of the power supply unit (2), so that the stage (7) is heated by the temperature A value, until the dwell time t A ends, a cycle is completed, and the counter D minus 1, and then enter the next cycle, until the counter D is zero, the experiment ends.
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Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 90205989 CN2067424U (en) | 1990-05-11 | 1990-05-11 | DNA augmentor for polymerase chain reaction |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 90205989 CN2067424U (en) | 1990-05-11 | 1990-05-11 | DNA augmentor for polymerase chain reaction |
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| Publication Number | Publication Date |
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| CN2067424U true CN2067424U (en) | 1990-12-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 90205989 Expired - Lifetime CN2067424U (en) | 1990-05-11 | 1990-05-11 | DNA augmentor for polymerase chain reaction |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102911863A (en) * | 2011-08-03 | 2013-02-06 | 常州福生生物技术有限公司 | Electric sample feeding device |
| CN105642382A (en) * | 2016-04-01 | 2016-06-08 | 东莞威铁克自动化科技有限公司 | Medical heating table |
-
1990
- 1990-05-11 CN CN 90205989 patent/CN2067424U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102911863A (en) * | 2011-08-03 | 2013-02-06 | 常州福生生物技术有限公司 | Electric sample feeding device |
| CN105642382A (en) * | 2016-04-01 | 2016-06-08 | 东莞威铁克自动化科技有限公司 | Medical heating table |
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