CN206318974U - A kind of modularization microalgae culture system for the device that quickly spread cultivation with algae kind - Google Patents

Abstract

A modular microalgae cultivation system with an algae rapid expansion device, including five algae expansion devices, photobioreactors, medium addition systems, gas delivery systems, algae liquid delivery and supernatant return systems module; the algae expansion device is connected with the medium addition system; the photobioreactor is connected with the algae expansion device, the medium addition system, the gas delivery system, the algae liquid delivery and the supernatant return system, and the photobioreactor It is set obliquely, and the algae seed expander is installed on the backlight side of the photoreactor. The system adopts a modular design, which is convenient for installation and adjustment. All modules are manufactured in advance and assembled on site. The materials between the modules are transported through pipe fittings during operation, which greatly reduces the difficulty of installation, maintenance and management, and can be adjusted according to the working conditions at any time. Adjusting the number of components of each unit to maintain production under optimal conditions can greatly increase the production of microalgae.

Description

A modular microalgae cultivation system with a rapid expansion device for algae species

technical field

The utility model relates to a cultivation system for realizing large-scale high-density and high-yield cultivation of microalgae. The invention belongs to the technical field of microalgae bioengineering.

Background technique

Microalgae is a kind of organism with rapid growth and strong environmental adaptability. Various biochemical substances contained in its biomass, such as oil, protein, carbohydrate and pigment, have high economic value and have great development prospects. Especially in recent years, microalgae have been considered as one of the most promising raw materials for the production of biofuels.

Photoautotrophic cultivation in photobioreactors is the main mode of microalgae cultivation. When large-scale cultivation is carried out, a large number of algae species are required. Traditionally, the production of algae species is achieved by microalgae under photoautotrophic conditions by means of step-by-step expansion cultivation. There are problems such as the expansion period is too long and it is easy to cause expansion failure due to pollution, and the provided cell density is low. It has become the main problem hindering the large-scale photoautotrophic cultivation of microalgae. Compared to photoautotrophic cultures, heterotrophic cultures have the advantages of high growth rates, high biomass yields, and avoidance of contamination by wild algae species. For example, adding a set of closed reactors to the microalgae culture system, in which microalgae cells are expanded heterotrophically as algae species, will undoubtedly greatly reduce the risk of failure in the expansion of algae species, shorten the expansion period and improve the efficiency of the photoreactor. cell density, thereby greatly improving the yield of microalgae.

The outdoor cultivation of microalgae faces large fluctuations in climate and environmental conditions, and the actual operating conditions are changeable, requiring frequent adjustments to each unit of the cultivation system.

Therefore, it is necessary to provide a modular microalgae cultivation system with a rapid expansion device for algae to reduce the difficulty of arranging, maintaining and adjusting the large-scale production system of microalgae, and greatly improve the biomass of microalgae, especially oil. yield.

Utility model content

The purpose of the utility model is to overcome the shortcomings of conventional microalgae cultivation reactors, and provide a modular microalgae cultivation system with a rapid expansion device for algae species, which is easy to install and adjust, and can greatly increase the production of microalgae.

The utility model has a modularized microalgae cultivation system with a rapid expansion device for algae species, and adopts the following technical solutions:

The system includes five modules of algae expansion device, photobioreactor, medium addition system, gas delivery system, algae liquid delivery and supernatant return system; the photobioreactor is inclined and the algae expansion device is installed On the backlight side of the photoreactor;

The algae seed expander includes a cavity of the expander, an intermediate diversion plate is arranged between the upper part and the lower part of the cavity, medium input ports communicating with the upper part of the cavity are respectively provided at both ends of the cavity, and the cavity The upper part of the body is provided with a stirrer, and the lower part of the cavity is provided with an algae seed liquid output port, which is connected to a flow controller, and the algae seed liquid output port is connected to the algae seed liquid input port on the photobioreactor. A metering peristaltic pump is connected to the algae liquid delivery pipe;

The photobioreactor includes a reactor cavity, and a diversion plate is arranged in the reactor cavity, and the reactor cavity is divided into left and right sides connected up and down, and an aeration hole plate is arranged on the left side, and the aeration hole The air holes are evenly distributed on the board, and the bottom of the left side is provided with an air inlet; the bottom of the right side is provided with the input port of the algae seed liquid, the return supernatant and the inlet of the culture medium; The algae liquid delivery pipe is connected; the top of the reactor cavity is provided with an exhaust port;

The medium adding system includes a nutrient storage tank, an organic matter storage tank and a medium storage tank, and the nutrient storage tank, the organic matter storage tank and the medium storage tank pass through the feed pipe and the medium input port on the algae expander respectively Connection, the medium storage tank is connected with the return supernatant on the photobioreactor and the medium inlet through the feed pipe, and all the feed pipes are connected with metering peristaltic pumps;

The gas delivery system includes _CO gas cylinders, air compressors, gas mixers and gas delivery pipes, _CO gas cylinders and air compressors are equipped with gas flowmeters, and the gas flowmeters are connected to the gas mixer, and the gas The mixer is connected with the gas delivery pipe, the gas delivery pipe is connected with a gas mass flow controller, and the gas delivery pipe is connected with the air inlet on the photobioreactor;

The algae liquid delivery and supernatant return system includes a solid-liquid separator, an algae liquid delivery pipe and a supernatant return pipe, the algae liquid delivery pipe is connected to the solid-liquid separator, and one end of the supernatant return pipe is connected to the solid-liquid return pipe. The separator is connected, and the other end is connected with the reflux supernatant and the medium inlet of the photobioreactor.

The photobioreactor is installed on a support, and an angle adjuster is provided between the photobioreactor and the support.

The included angle between the photobioreactor and the horizontal plane is 50°-70°.

The outlet of the algae seed liquid in the algae seed expander faces to the side of the photoreactor.

An algae liquid drainage plate is arranged at the outlet of the algae liquid in the reactor cavity.

A liquid level sensor is installed in the upper part of the reactor cavity to prevent the liquid level from leaking through the exhaust port if the liquid level is too high.

The utility model can be used for the large-scale cultivation of microalgae and the production of related microalgae bioenergy; it can cultivate microalgae that can be cultured heterotrophically and grow rapidly, such as Chlorella, Scenedesmus tetracinus, Scenedesmus obliquus, Chrysophytes, Phaeodactylum tricornutum, diatoms, etc.; have the following characteristics:

1. Modular design is adopted, all modules are manufactured in advance, assembled on site, fixed with brackets, and the materials between modules are transported through pipe fittings during operation, which greatly reduces the difficulty of installation, maintenance and management, and can be adjusted according to working conditions at any time Adjust the number of components of each unit to maintain production under optimal conditions.

2. The modules are connected by pipe fittings, and the relative position freedom is large, which can make full use of the site space.

3. Using an independent heterotrophic algae expansion device, which runs simultaneously with the photobioreactor, greatly reduces the risk of algae expansion failure, shortens the expansion cycle and increases the cell density in the photoreactor to 8-10g L ^-1 , can increase the biomass yield by 4-6 times.

4. Heterotrophic/nitrogen-enriched and autotrophic/nitrogen-deficient cultures are used in the algae expander and photobioreactor respectively, which greatly increases the oil content, and the oil yield when cultivating Chlorella is 10 times that of ordinary photobioreactors about times.

5. Use the aeration device to circulate the algae liquid in the reactor, which can play a good role in mixing the algae liquid, supplementing the inorganic carbon source and removing the supersaturated oxygen, preventing the growth of algae cells attached to the wall, and reducing the supersaturated oxygen Toxic to algal cells.

6. Most of the supernatant after the harvest of microalgae is returned to the reactor, which saves the usage of water and various chemical reagents, and reduces the discharge of waste water; the liquid level sensor is installed inside the reactor to prevent the liquid level from being too high Vent leaks.

7 Both the metering pump and the gas flow meter can be controlled by a microcomputer, which can realize both manual control and automatic control.

Description of drawings

Fig. 1 is the planar structure schematic diagram of the present utility model,

Fig. 2 is a schematic side view of the utility model.

Fig. 3 is the structural representation of photobioreactor in the utility model,

Fig. 4 is a structural schematic diagram of the algae seed expanding device in the utility model.

Fig. 5 is a side sectional view of the algae seed expander in the utility model.

In the figure: 1. Algae solution delivery pipe; 2. Supernatant liquid return pipe; 3. Culture medium feeding pipe; 4. Photobioreactor; 5. Medium conveying pipeline; 6. Nitrate solution feeding pipe; 7. Glycerin solution feed pipe; 8. Algae seed expander; 9. Algae seed liquid delivery pipe; 10. Nitrate solution storage tank; 11. Glycerin solution storage tank; 12. Medium storage tank; 13. Support; 14. CO ₂ cylinder; 15. Gas mixer; 16. Air compressor; 17. Gas delivery pipeline; 18. Reactor cavity; 19. Exhaust port; 20. Liquid level sensor; Plate; 22. Algae solution drainage plate; 23. Algae solution output port; 24. Algae seed solution input port; 25. Backflow supernatant and culture medium inlet; 26. Aeration orifice plate; 27. Air inlet; 28. Culture medium input port; 29. expander cavity; 30. agitator; 31. intermediate diversion plate; 32. algae liquid (algae liquid containing algae cells) output port.

detailed description

As shown in Figure 1 and Figure 2, the modular microalgae cultivation of the present utility model. It includes five modules of an algae seed expander 8, a photobioreactor 4, a medium addition system, a gas delivery system, and an algae liquid delivery and supernatant return system. The photobioreactor 4 is installed on the bracket 13, and the bracket 13 has an angle adjuster for adjusting the inclination angle of the photobioreactor 4. The included angle between the photobioreactor 4 and the horizontal plane is 50-70°. The algal seed expander 8 is installed on the backlight side of the photoreactor 4 . All modules are pre-manufactured and assembled on site, and the substances between the modules are transported through pipe fittings during operation. Microalgae that can be cultured heterotrophically and have rapid biology can all be cultivated in the utility model, such as Chlorella, Scenedesmus tetrasaurus, Scenedesmus obliquus, Chrysophytes, Phaeodactylum tricornutum, diatoms, etc., which can be used for microalgae Large-scale cultivation and production of related microalgal bioenergy.

The structure of the algae expander 8 is shown in Fig. 4 and Fig. 5, which includes an expander cavity 29, and an intermediate diversion partition 31 is arranged between the upper part and the lower part of the cavity 29. Both ends of the cavity 29 are respectively provided with medium input ports 28 communicating with the upper part of the cavity, and the upper part of the cavity 29 is provided with multiple groups of agitators 30 arranged at intervals. The lower part of the cavity 29 is provided with a plurality of algal seed liquid output ports 32, and a flow controller is connected to the algal seed liquid output ports 32. The algae liquid output port 32 is towards the photoreactor 4 side, and an algae liquid output port 32 is connected with a photoreactor 4, and the algae liquid output port 32 is connected to the photobioreactor 4 through the algae liquid delivery pipe 9. The algae seed liquid input port 24 is connected (referring to Fig. 1), and the algae seed liquid conveying pipe 9 is connected with a metering peristaltic pump.

The structure of the photobioreactor 4 is shown in Figure 3, including the reactor chamber 18, which is made of light-transmitting plexiglass and has better performance. The middle of the cavity 18 is provided with a deflector baffle 21, which divides the cavity 18 into two parts, the left side and the right side, which communicate up and down. Air holes are evenly distributed on the plate 26 . There is a cavity between the aeration hole plate 26 and the cavity 18 . An air inlet 27 is provided at the left bottom of the cavity 18 . The bottom of the right side of the cavity 18 is provided with an input port 24 for the algae seed liquid and an inlet 25 for the return supernatant and the culture medium. The return supernatant and the culture medium inlet 25 are respectively connected with the supernatant return pipe 2 and the culture medium feed pipe 3 (see FIG. 1 ), and the culture medium feed pipe 3 is connected with a metering peristaltic pump. The right side of the cavity 18 is provided with an algae liquid outlet 23 , and an algae liquid drainage plate 22 is arranged at the algae liquid output port 23 in the cavity 18 . The algae liquid output port 23 is connected with the algae liquid delivery pipe 1 to be harvested (see FIG. 1 ). The top of the cavity 18 is provided with an exhaust port 19 , and the exhaust port 19 is obliquely opposite to the aeration hole plate 26 . A liquid level sensor 20 is installed on the upper part of the cavity 18 to prevent the liquid level from leaking through the exhaust port when the liquid level is too high.

The medium adding system includes a nitrate solution storage tank 10, a glycerol solution storage tank 11 and a medium storage tank 12, see FIG. 1 . The nitrate solution storage tank 10 is connected with the medium input port 28 on the algae expansion device 8 by the nitrate solution feed pipe 6 and the metering peristaltic pump, and the glycerin solution storage tank 11 is passed through the glycerol solution feed pipe 7 and the metering peristaltic pump It is connected with the medium input port 28 on the algal seed expander 8. The medium storage tank 12 is connected with the medium input port 28 on the algae seed expander 8 through the medium delivery pipe 5 and the metering peristaltic pump. Nitrate solution, glycerol solution and culture medium are mixed into the algae expanding device 8. At the same time, the medium storage tank 12 is connected with the reflux supernatant on the photobioreactor 4 and the medium inlet 25 through the medium feed pipe 3 . Nitrate (usually sodium nitrate solution) as a nutrient. Glycerin solution is input into the algae expanding device 8 as organic matter, and the input amount is controlled by a metering peristaltic pump.

The gas delivery system includes a _CO2 gas cylinder 14, an air compressor 16, a gas mixer 15 and a gas delivery tube 17, see FIG. 1 . _CO Gas cylinder 14 and air compressor 16 are equipped with gas flowmeters, and the gas flowmeters on _CO gas cylinder 14 and air compressor 16 are all connected to gas mixer 15, and gas mixer 15 is connected to gas delivery pipe 17 connect. A gas mass flow controller is connected to the gas delivery pipe 17 , and the gas delivery pipe 17 is connected to the air inlet 27 of the photobioreactor 4 . CO ₂ , sterilized air or a mixture of CO ₂ and sterilized air delivered by an air pump or a gas cylinder is passed into the photobioreactor 4 as a gas source.

The algae liquid delivery and supernatant return system includes a solid-liquid separator, an algae liquid delivery pipe 1 and a supernatant return pipe 2, and the solid-liquid separator is not shown in the figure. The algae liquor conveying pipe 1 is connected with the solid-liquid separator, and transports the algae liquor to the solid-liquid separation equipment. One end of the supernatant return pipe 2 is connected to the solid-liquid separator, and the other end is connected to the return supernatant of the photobioreactor 4 and the medium inlet 25, and most of the supernatant is returned to the photobioreactor 4.

A set of modular microalgae cultivation system can be equipped with a set of algae expanders 8, 1-2 sets of medium addition systems, a set of gas delivery systems and 1-10 sets of photobioreactors 4, each set of photobioreactors 4 Configure a set of algae liquid delivery and supernatant return system and a set of mounting brackets. The specific quantity and installation location of components are flexibly controlled by site conditions and production requirements.

The algae seed expander 8 is one of the main modules of the microalgae cultivation system. The expander cavity 29 is a biological reaction space for algae proliferation. The culture medium that has mixed the nitrate solution and the glycerin solution enters in the expander cavity 29 from the culture medium input port 28, mixes with the original algae seed liquid immediately after stirring in the agitator 30, and in the middle diversion plate 31 Under the guidance of the expander cavity 29, it continuously circulates and proliferates; part of the algae seed liquid is exported as algae seed from the algae seed liquid output port 32, and is transported by the algae seed liquid delivery pipe 9 and the metering peristaltic pump. The liquid input port 24 enters the photobioreactor 4 . In the utility model, the algae expansion device under heterotrophic conditions is used to provide algae species for the photobioreactor, which greatly reduces the risk of algae expansion failure, shortens the expansion period and increases the cell density in the photoreactor, thereby extremely Greatly increase the yield of microalgae.

The photobioreactor 4 is one of the main modules of the microalgae cultivation system. The cavity in the reactor cavity 18 is a biological reaction space for the growth of microalgae. The algae seed liquid enters the reactor chamber 18 from the algae seed liquid input port 24, and the backflow supernatant and the culture medium enter the reactor chamber 24 through the backflow supernatant and the culture medium inlet 8, and are immediately mixed with the original algae liquid. The mixed gas enters the reactor cavity 18 from the air inlet 27, forms fine bubbles through the aeration orifice 26, aerates and stirs the algae liquid, and forms a circulating flow under the guidance of the diversion plate 21, and rises to the liquid The gas on the surface is discharged through the exhaust pipe 19. The rate of intake and liquid intake is controlled by feedback from the liquid level sensor 20 . Part of the algae liquid circulated to the non-aeration side is discharged from the algae liquid output port 23 through the algae liquid delivery pipe 1 under the guidance of the algae liquid diversion plate 22, as the algae liquid to be harvested. Using the aeration hole plate instead of the quartz aeration head can effectively improve the uniformity of aeration, increase the utilization rate of microalgae to gas, and reduce the interference of turbulent flow on the growth of microalgae, thereby improving the growth efficiency of microalgae, thereby improving the efficiency of the reactor. The concentration of microalgae in the medium reduces algal adherent growth. The diversion partition 21 is set, on the one hand, the mechanical strength of the reactor is improved, and on the other hand, with the cooperation of one-sided aeration, the circulation of the algae liquid in the reactor is formed, which is beneficial to the contact between the algae cells and the gas and nutrients , Increased mass transfer efficiency and raw material utilization efficiency. Feedback information from the liquid level sensor 20 is used to automatically control the intake and liquid intake rate, stabilize the liquid level, and prevent unfavorable operations such as liquid flooding.

During operation, the CO ₂ in the CO ₂ gas cylinder 14 and the air output from the air compressor 16 are mixed in proportion by the gas flow meter and then mixed by the gas mixer 15 , and then input into the photobioreactor 4 through the gas delivery pipe 17 . The algal liquid to be harvested outputted by the photobioreactor 4 is sent to the algae liquid conveying pipe 1 for solid-liquid separation, and most of the supernatant after the solid-liquid separation flows back into the photobioreactor 4 .

Claims (6)

1. A modular microalgae cultivation system with a rapid expansion device for algae, characterized in that it includes an expansion device for algae, a photobioreactor, a medium addition system, a gas delivery system, and an algae liquid delivery and upper There are five modules in the clear liquid reflux system; the photobioreactor is installed obliquely, and the algae expansion device is installed on the backlight side of the photoreactor; The algae seed expander includes a cavity of the expander, an intermediate diversion plate is arranged between the upper part and the lower part of the cavity, medium input ports communicating with the upper part of the cavity are respectively provided at both ends of the cavity, and the cavity The upper part of the body is provided with a stirrer, and the lower part of the cavity is provided with an algae seed liquid output port, which is connected to a flow controller, and the algae seed liquid output port is connected to the algae seed liquid input port on the photobioreactor. A metering peristaltic pump is connected to the algae liquid delivery pipe; The photobioreactor includes a reactor cavity, and a diversion plate is arranged in the reactor cavity, and the reactor cavity is divided into left and right sides connected up and down, and an aeration hole plate is arranged on the left side, and the aeration hole The air holes are evenly distributed on the board, and the bottom of the left side is provided with an air inlet; the bottom of the right side is provided with the input port of the algae seed liquid, the return supernatant and the inlet of the culture medium; The algae liquid delivery pipe is connected; the top of the reactor cavity is provided with an exhaust port; The medium adding system includes a nutrient storage tank, an organic matter storage tank and a medium storage tank, and the nutrient storage tank, the organic matter storage tank and the medium storage tank pass through the feed pipe and the medium input port on the algae expander respectively Connection, the medium storage tank is connected with the return supernatant on the photobioreactor and the medium inlet through the feed pipe, and all the feed pipes are connected with metering peristaltic pumps; The gas delivery system includes _CO gas cylinders, air compressors, gas mixers and gas delivery pipes, _CO gas cylinders and air compressors are equipped with gas flowmeters, and the gas flowmeters are connected to the gas mixer, and the gas The mixer is connected with the gas delivery pipe, the gas delivery pipe is connected with a gas mass flow controller, and the gas delivery pipe is connected with the air inlet on the photobioreactor; The algae liquid delivery and supernatant return system includes a solid-liquid separator, an algae liquid delivery pipe and a supernatant return pipe, the algae liquid delivery pipe is connected to the solid-liquid separator, and one end of the supernatant return pipe is connected to the solid-liquid return pipe. The separator is connected, and the other end is connected with the reflux supernatant and the medium inlet of the photobioreactor. 2. The modularized microalgae cultivation system with the rapid expansion device for algae species according to claim 1, characterized in that, the photobioreactor is installed on the support, with a belt between the photobioreactor and the support Angle adjuster. 3. The modularized microalgae cultivation system with a rapid expansion device for algae species according to claim 1, wherein the angle between the photobioreactor and the horizontal plane is 50°-70°. 4 . The modularized microalgae cultivation system with a rapid expansion device for algae according to claim 1 , wherein the outlet of the liquid for algae in the expansion device for algae faces toward the side of the photoreactor. 5 . The modularized microalgae cultivation system with an algae rapid expansion device according to claim 1 , wherein an algae liquid drainage plate is arranged at the outlet of the algae liquid in the reactor cavity. 6 . 6 . The modularized microalgae cultivation system with an algae rapid expansion device according to claim 1 , wherein a liquid level sensor is installed on the upper part of the reactor cavity. 7 .