CN206161536U - Quick detect reagent box of grain in storage for years - Google Patents

Abstract

The utility model discloses a quick detection kit for aged grain, which belongs to the technical field of food safety detection kits. The key points of the technical solution of the utility model are: a rapid detection kit for aged grain, including a box body and a mold with a groove inside the box body, wherein the mold is provided with a concave bottle position for eluent and a display indicator Downward concave bottle position, centrifuge tube groove, suction pipe groove, sampling spoon groove and colorimetric card groove, the eluent downward concave bottle position, indicator indicator downward concave bottle position, centrifuge tube groove, suction pipe groove, sampling spoon groove and comparison card groove The eluent bottle, display indicator bottle, centrifuge tube, straw, sampling spoon and color card are placed in the color card slot. Compared with other detection methods, the utility model has simple operation and low price, and can be quickly, conveniently, sensitively and accurately used for qualitative detection of the freshness of aged grains in food.

Description

A rapid detection kit for aged grain

technical field

The utility model belongs to the technical field of food safety detection kits, in particular to a rapid detection kit for aged grains.

Background technique

Aged grain refers to the grain that has been stored for a long time, and its Aspergillus flavus bacteria exceeds the standard, and it can no longer be directly used as ration grain. The state stipulates that aged grains can only be sold to specific feed processing enterprises and brewing companies through auctions, and used in strict accordance with the regulations. Reselling and changing purposes without authorization are illegal. Aged grains are different from what people call "second-year grains", "every year grains" and "thread rice". Aged grains need to undergo strict testing and identification before they can be identified as aged grains.

Symptoms such as vomiting, fever, abdominal pain, and loss of appetite may occur after eating aged food products that are seriously polluted by aflatoxins, etc. In severe cases, hepatosplenomegaly, pain in the liver area, yellow staining of skin and mucous membranes, and Accumulation of ascites, lower extremity edema, abnormal liver function and other symptoms of poisoning may also occur, such as cardiac expansion, pulmonary edema, convulsions and coma. In 2004, in some grain and oil wholesale markets across the country, a kind of rice called "migrant workers' rice" appeared one after another, which is exactly the aged grain that is strictly prohibited by the state. The harm of aged grain to citizens is not only the aflatoxin and its metabolites contained in it itself, but also because illegal personnel add carcinogen "hangbaikuai" to improve the color of inferior rice noodles, which increases the risk of cancer for the public .

The main detection indicators to detect whether the rice is "aged" are: fatty acid value, viscosity, taste score value and color and smell. Among them, if one of the three indicators of fatty acid value, viscosity, and taste score value meets the "aging" requirement, it is deemed It is "Chen Hualiang". Scholars at home and abroad have done a lot of research on the detection method of rice freshness, the purpose is to guide the storage of rice (storage quality judgment), the regulation of storage conditions and the quality inspection of import and export. At present, the detection methods of rice freshness mainly include: guaiacol reaction method, guaiacol-p-phenylenediamine combined method, falling number determination method, fatty acid value determination method, gelatinization viscosity determination method and acidity indicator Among them, the spectrophotometric method of acid indicator and the determination method of fatty acid value are more accurate, and the comprehensive application of pH measurement method, spectrophotometry method of acid indicator and fatty acid value determination method can obtain a more comprehensive evaluation of rice freshness. evaluation of.

The latest national standard GB/T 15684-2015 is the national standard of the People's Republic of China. It has formulated the method for the determination of fatty acid value in grain and oil crops, and specified the operation steps and scope of application in detail. This method is accurate and comprehensive, and can determine the residual content of fatty acid, but It requires certain instruments and equipment, cumbersome and complicated operating procedures, high costs and long inspection time (about 4 hours). National standards cannot be operated on site, and it is difficult to meet actual monitoring needs. Therefore, it is of great significance to develop a fast, sensitive and efficient aged grain detection kit to ensure food safety.

Contents of the invention

The technical problem solved by the utility model is to provide a small and portable aging grain rapid detection kit, which is easy to operate, fast in detection, low in cost and good in stability, and can carry out continuous measurement of multiple samples at one time, reducing the the time required to test the sample.

In order to solve the above technical problems, the utility model adopts the following technical scheme, a rapid detection kit for aged grain, which is characterized in that it includes a box body and a mold with a groove inside the box body, wherein the mold is provided with an eluent bottom Concave bottle position, display indicator concave bottle position, centrifuge tube slot, suction pipe slot, sampling spoon slot and colorimetric card slot, the eluent concave bottle position, display indicator concave bottle position, centrifuge tube slot, straw The eluent bottle, display indicator bottle, centrifuge tube, straw, sampling spoon and color comparison card are correspondingly placed in the groove, sampling spoon groove and color comparison card groove.

Further preferably, the box body is made of cardboard or hard plastic, and its exterior is covered by an adhesive film, with a length of 20-25 cm, a width of 12-16 cm, and a height of 12-15 cm.

Further preferably, the two sides of the mold are respectively provided with a concave bottle position for rice eluent, a concave bottle position for wheat eluent, a concave bottle position for rice flour eluent and a concave bottle position for rice cake eluent, wherein the rice The concave bottle position of the eluent and the concave bottle position of the wheat eluent are set symmetrically with the concave bottle position of the rice flour eluent and the concave bottle position of the rice cake eluent, and the concave bottle position of the rice eluent and the wheat eluent Rice eluent bottle, wheat eluent bottle, rice flour eluent bottle and rice cake eluent bottle are respectively placed in the concave bottle position, rice flour eluent concave bottle position and rice cake eluent concave bottle position.

Further preferably, the bottle body and bottle cap of the rice eluent bottle are both black, the bottle body and bottle cap of the wheat eluent bottle are both red, and the bottle body and bottle cap of the rice flour eluent bottle are blue The body and cap of the rice cake eluent bottle are brown.

Further preferably, the middle part of the mold is provided with a display indicator concave bottle position, and a 100mL display indicator bottle is placed in the display indicator concave bottle position, and the bottle body of the display indicator bottle is a dark purple plastic bottle. The bottle cap is purple, indicating that the indicator is recessed and equipped with 2 indicator burettes.

Further preferably, 100 disposable plastic centrifuge tubes are arranged in the centrifuge tube groove, the centrifuge tube cover is one-piece or twisted, the bottom of the centrifuge tube is flat, round or pointed, and the volume of the centrifuge tube is 3mL, 4mL, 5mL or 6mL.

Further preferably, the said color comparison card slot is provided with 5 color comparison cards encapsulated by film and film, and the color comparison cards are provided with a color band gradually changing from dark green to dark red.

The utility model uses plastic bottles of different sizes and colors to contain reagents and fixes them in foam plastic molds, and is packaged in a cardboard box together with centrifuge tubes, sampling spoons, straws, color cards and instructions, so as to be easy to carry and transport . The reagents in each kit are enough to carry out 100 determinations, which can be used for continuous detection of multiple samples at one time, and can also be used in batches for multiple times. During detection, the eluent extracts the active ingredients in the sample, and then adds a color indicator. According to the amount of active ingredient extract in the sample, the eluent will show different colors, and then compare it with the color comparison card. Easily judge the freshness of aged grain (current year, one year, two years, three years and above) or the condition of aging.

Compared with other detection methods, the utility model has simple operation and low price. The beneficial effect of the utility model is that it can be quickly, conveniently, sensitively and accurately used for qualitative detection of the freshness of aged grains in food.

Description of drawings

Fig. 1 is a structural representation of the utility model;

Fig. 2 is the structural representation of mold in the utility model;

Fig. 3 is the structural representation of rice eluent bottle in the utility model;

Fig. 4 is the structural representation of the color comparison card in the utility model.

In the figure: 1. Box body, 2. Mold, 3. The concave bottle position of rice eluent, 4. The concave bottle position of wheat eluent, 5. The concave bottle position of rice flour eluent, 6. Rice cake elution Concave bottle position under liquid, 7. Concave bottle position for display indicator, 8. Centrifuge tube groove, 9. Suction pipe groove, 10. Sampling spoon groove, 11. Colorimetric card groove, 12. Bottle body, 13. Bottle cap, 14. Color comparison card.

detailed description

The specific content of the utility model is described in detail in conjunction with the accompanying drawings. A rapid detection kit for aged grains, comprising a box body 1 and a mold 2 with grooves arranged inside the box body 1, wherein the mold 2 is provided with a concave bottle position for eluent and a concave bottle position for display indicator 7 , centrifuge tube groove 8, suction pipe groove 9, sampling spoon groove 10 and colorimetric card groove 11, the concave bottle position of the eluent, the concave bottle position 7 of the display indicator, the centrifuge tube groove 8, the suction pipe groove 9, the sampling spoon An eluent bottle, a display indicator bottle, a centrifuge tube, a straw, a sampling spoon and a color comparison card 14 are correspondingly placed in the slot 10 and the color comparison card slot 11 .

Described box body 1 is made by cardboard or hard plastic, and its exterior is covered by film, and length is 22cm, and width is 15cm, and height is 12cm.

Both sides of the mold 2 are respectively provided with a concave bottle position 3 for rice eluent, a concave bottle position 4 for wheat eluent, a concave bottle position 5 for rice flour eluent, and a concave bottle position 6 for rice cake eluent. Wherein the concave bottle position 3 of the rice eluent and the concave bottle position 4 of the wheat eluent are arranged symmetrically with the concave bottle position 5 of the rice flour eluent and the concave bottle position 6 of the rice cake eluent, and the concave bottle position of the rice eluent Position 3, the concave bottle position 4 of wheat eluent, the concave bottle position 5 of rice flour eluent, and the concave bottle position 6 of rice cake eluent are respectively placed with rice eluent bottle, wheat eluent bottle, and rice flour washing liquid. Eluent bottle and rice cake eluent bottle.

The bottle body 12 and bottle cap 13 of the rice eluent bottle are black, the bottle body and bottle cap of the wheat eluent bottle are red, and the bottle body and bottle cap of the rice flour eluent bottle are blue , the body and cap of the rice cake eluent bottle are brown.

The middle part of the mold 2 is provided with a display indicator concave bottle position 7, and a 100mL display indicator bottle is placed in the display indicator concave bottle position 7, and the bottle body of the display indicator bottle is a deep purple plastic bottle body. The bottle cap is purple, showing that the indicator is recessed. The bottle position 7 is also equipped with 2 indicator burettes.

Described centrifuge tube tank 8 is provided with 100 disposable plastic centrifuge tubes, the centrifuge tube cover is one-piece or screw type, the bottom of the centrifuge tube is flat bottom, round bottom or pointed bottom, and the volume of the centrifuge tube is 3mL, 4mL, 5mL or 6mL.

The color comparison card slot 11 is provided with 5 color comparison cards 14 encapsulated by plastic film, and the color comparison cards 14 are provided with a color band gradually changing from dark green to dark red, showing a gradual change trend.

Example 1

Detection of aged rice grains: 1. The sampling spoon collects about 20 rice grains to be inspected, and puts them into the bottom of the centrifuge tube; 2. Takes about 1.5mL of rice eluent through the straw, and adds it to the centrifuge tube; 3. Tighten the lid, shake vigorously up and down, left and right 0.5min; 4. After the eluate is allowed to stand still, add 2 drops of color indicator with a dropper; 5. After standing still for 0.5min, compare the color of the eluate in the centrifuge tube with the color comparison card to judge whether the rice is new or old.

Example 2

For the detection of aged wheat grains, 1. The sampling spoon collects about 15 wheat grains to be inspected, and puts them into the bottom of the centrifuge tube; 2. Draws about 1.5mL of wheat eluent through the straw, and adds it to the centrifuge tube; 3. Tighten the lid, and shake vigorously up and down, left and right 0.5min; 4. After the eluate is allowed to stand still, add 2 drops of color indicator with a dropper; 5. After standing still for 0.5min, compare the color of the eluate in the centrifuge tube with the color comparison card to judge whether the wheat is new or old.

Example 3

For the detection of rice flour aged grains, 1. The sampling spoon collects about 500 mg of rice flour to be tested, and puts it into the bottom of the centrifuge tube; 2. Takes about 2 mL of the rice flour eluent through the straw, and adds it to the centrifuge tube; 3. Tighten the lid, and shake vigorously up and down, left and right for 0.5 minutes; 4. After the eluate is left to stand, add 3 drops of color indicator with a dropper; 5. After standing for 0.5 minutes, compare the color of the eluate in the centrifuge tube with the color chart to judge the degree of rice noodles.

Example 4

For the detection of rice cake aged grains, 1. Collect about 500 mg of rice cake to be tested with a sampling spoon, and put it into the bottom of the centrifuge tube; 2. Take about 2 mL of rice cake eluent with a straw, and add it to the centrifuge tube; 3. Tighten the lid, shake vigorously up and down, left and right for 0.5 minutes; 4. After the eluate is left to stand, add 3 drops of color indicator with a dropper; 5. After standing for 0.5 minutes, compare the color of the eluate in the centrifuge tube with the color chart to judge the aging degree of the rice cake raw material.

Precautions:

1. After the reagent is used, the cap of the eluent bottle and the cap of the indicator bottle must be tightened;

2. The test kit is stored at room temperature, and the shelf life is one year;

3. Centrifuge tubes, sampling spoons and straws are disposable tools and cannot be reused;

4. When collecting and testing samples, it is best to measure the accuracy in triplicate;

5. This kit is a qualitative test, and the determination of the residual fatty acid content of aged grains needs to be verified by the national standard method.

The basic principles, main features and advantages of the present utility model have been shown and described above. On the premise of not departing from the spirit and scope of the present utility model, the present utility model also has various changes and improvements, and these changes and improvements all fall into the claims. The scope of the utility model.

Claims (7)

1. A rapid detection kit for aged grains, characterized in that it comprises a box body and a mold with grooves arranged inside the box body, wherein the mold is provided with a concave bottle position for eluent and a concave bottle position for display indicator , centrifuge tube groove, suction pipe groove, sampling spoon groove and colorimetric card groove, the concave bottle position of the eluent, the concave bottle position of the display indicator, centrifuge tube groove, suction pipe groove, sampling spoon groove and colorimetric card groove Correspondingly, there are eluent bottles, display indicator bottles, centrifuge tubes, straws, sampling spoons and color comparison cards. 2. The aged grain rapid detection kit according to claim 1, characterized in that: the box body is made of cardboard or hard plastic, the outside is covered by a film, the length is 20-25cm, and the width is 20-25cm. It is 12-16cm and the height is 12-15cm. 3. The aging grain rapid detection kit according to claim 1, characterized in that: the two sides of the mold are respectively provided with a concave bottle position for rice eluent, a concave bottle position for wheat eluent, and a rice flour washing liquid. The concave bottle position of the dehydration liquid and the concave bottle position of the rice cake eluent, the concave bottle position of the rice eluent and the concave bottle position of the wheat eluent and the concave bottle position of the rice flour eluent and the concave bottle of the rice cake eluent The bottle positions are arranged symmetrically, and rice eluent bottles, rice eluent bottles, Wheat wash bottle, rice flour wash bottle, and rice cake wash bottle. 4. Aged grain quick detection kit according to claim 3, is characterized in that: the bottle body and bottle cap of described rice eluent bottle are black, and the bottle body and bottle cap of wheat eluent bottle Both are red, the bottle body and bottle cap of the rice flour eluent bottle are blue, and the bottle body and bottle cap of the rice cake eluent bottle are brown. 5. The aging grain rapid detection kit according to claim 1, characterized in that: the middle part of the mold is provided with a concave bottle position of a display indicator, and a 100mL display indicator is placed in the concave bottle position of the display indicator Indicator bottle, showing that the bottle body of the indicator bottle is a deep purple plastic bottle body, the bottle cap is purple, and there are two indicator burettes on the concave bottle position showing the indicator. 6. The aging grain rapid detection kit according to claim 1, characterized in that: 100 disposable plastic centrifuge tubes are arranged in the centrifuge tube tank, and the centrifuge tube covers are one-piece or twisted, The bottom of the centrifuge tube is flat bottom, round bottom or pointed bottom, and the volume of the centrifuge tube is 3mL, 4mL, 5mL or 6mL. 7. The aging grain rapid detection kit according to claim 1, characterized in that: the colorimetric card slot is provided with 5 colorimetric cards packaged in plastic film, and the colorimetric card is provided with A color band that gradually changes from dark green to dark red.